MtDNA was successfully extracted from ten individual bones (femurs) in the tombs of ancient Jushi in Turfan basin, dated back to the year about 3 000-2 500 years ago. By means of four overlapping primers, we got nucl...MtDNA was successfully extracted from ten individual bones (femurs) in the tombs of ancient Jushi in Turfan basin, dated back to the year about 3 000-2 500 years ago. By means of four overlapping primers, we got nucleotide sequence of the 218bp length. Ancient mtDNA was analyzed by the sequencing of hypervariable region Ⅰ of the mtDNA control region. The result shows that 9 haplotypes with 24 polymorphic sites were obtained. The phylogenetic analysis indicated that Mongolians and Altai are the population genetically closest to the Jushi groups and Jushi mtDNA pool being an admixture of eastern Asian and European lineages. So our preliminary data imply that an ancient mingling of Euro-Asian population had existed in Turfan basin prior to the early Iron Age.展开更多
Ancient DNA was extracted from 13 skeletal remains from the burial groups of Khitan nobles, which were excavated in northeast China. The hypervariable segment Ⅰ sequences （ HVS Ⅰ ） of the mitochondrial DNA control...Ancient DNA was extracted from 13 skeletal remains from the burial groups of Khitan nobles, which were excavated in northeast China. The hypervariable segment Ⅰ sequences （ HVS Ⅰ ） of the mitochondrial DNA control region, in the 13 individuals, were used as genetic markers to determine the genetic relationships between the individuals and the genetic affinity to other interrelated populations by using the known database of mtDNA. Based on the phylogenetic analysis of these ancient DNA sequences, the genetic structures of two Khitan noble kindreds were obtained, including the Yel Yuzhi＇s kindred and the Xiao He＇s kindred. Furthermore, the relationships between the Khitan nobles and some modern interrelated populations were analyzed. On the basis of the result of the analysis, the gene flows of the ancient Khitans and their demographic expansion in history was deduced.展开更多
Fibroblast growth factor（FGF） signal transduction pathway is correlated with many diseases, such as cancer, skeletal dysplasias, olfactory syndromes and so on. P（VYMSPF）, a small peptide, is an antagonist of aFGF....Fibroblast growth factor（FGF） signal transduction pathway is correlated with many diseases, such as cancer, skeletal dysplasias, olfactory syndromes and so on. P（VYMSPF）, a small peptide, is an antagonist of aFGF. In order to investigate the impact of the peptide P on aFGF signal transduction pathway, the proteomes of NIH3T3 cell stimulated under different conditions（aFGF＋heparin sodium and aFGF＋heparin sodium＋P） were extracted and analyzed by 2DE, five differentially expressed protein spots were selected and identified via ESI-MS/MS. The result indicates that the peptide P has a great influence on the function of aFGF such as metabolism, protein translation and cytoskeleton formation; the target signal pathway of the peptide P may be MAPK pathway and the peptide P might also affect the PLC7 pathway. The article provides a new insight into the peptide P as a potential anticancer drug.展开更多
基金Supported by Fund for the Important Project of the Science and Technology DepartmentMinistry of Education
文摘MtDNA was successfully extracted from ten individual bones (femurs) in the tombs of ancient Jushi in Turfan basin, dated back to the year about 3 000-2 500 years ago. By means of four overlapping primers, we got nucleotide sequence of the 218bp length. Ancient mtDNA was analyzed by the sequencing of hypervariable region Ⅰ of the mtDNA control region. The result shows that 9 haplotypes with 24 polymorphic sites were obtained. The phylogenetic analysis indicated that Mongolians and Altai are the population genetically closest to the Jushi groups and Jushi mtDNA pool being an admixture of eastern Asian and European lineages. So our preliminary data imply that an ancient mingling of Euro-Asian population had existed in Turfan basin prior to the early Iron Age.
文摘Ancient DNA was extracted from 13 skeletal remains from the burial groups of Khitan nobles, which were excavated in northeast China. The hypervariable segment Ⅰ sequences （ HVS Ⅰ ） of the mitochondrial DNA control region, in the 13 individuals, were used as genetic markers to determine the genetic relationships between the individuals and the genetic affinity to other interrelated populations by using the known database of mtDNA. Based on the phylogenetic analysis of these ancient DNA sequences, the genetic structures of two Khitan noble kindreds were obtained, including the Yel Yuzhi＇s kindred and the Xiao He＇s kindred. Furthermore, the relationships between the Khitan nobles and some modern interrelated populations were analyzed. On the basis of the result of the analysis, the gene flows of the ancient Khitans and their demographic expansion in history was deduced.
基金Supported by the Science and Technology Development Project of Jilin Province,China(No.20040411-3)
文摘Fibroblast growth factor（FGF） signal transduction pathway is correlated with many diseases, such as cancer, skeletal dysplasias, olfactory syndromes and so on. P（VYMSPF）, a small peptide, is an antagonist of aFGF. In order to investigate the impact of the peptide P on aFGF signal transduction pathway, the proteomes of NIH3T3 cell stimulated under different conditions（aFGF＋heparin sodium and aFGF＋heparin sodium＋P） were extracted and analyzed by 2DE, five differentially expressed protein spots were selected and identified via ESI-MS/MS. The result indicates that the peptide P has a great influence on the function of aFGF such as metabolism, protein translation and cytoskeleton formation; the target signal pathway of the peptide P may be MAPK pathway and the peptide P might also affect the PLC7 pathway. The article provides a new insight into the peptide P as a potential anticancer drug.
