Knockdown of circular RNA (CircRNA)_001896 inhibits cervical cancer proliferation and stemness in vivo and in vitro

Objective:Previous studies indicated that aberrant circular RNA(circRNA)expression affects gene expression regulatory networks,leading to the aberrant activation of tumor pathways and promoting tumor cell growth.However,the expression,clinical significance,and effects on cell propagation,invasion,and dissemination of circRNA_001896 in cervical cancer(CC)tissues remain unclear.Methods:The Gene Expression Omnibus(GEO)datasets(GSE113696 and GSE102686)were used to examine differential circRNA expression in CC and adjacent tissues.The expression of circRNA_001896 was detected in 72 CC patients usingfluorescence quantitative PCR.Correlation analysis with clinical pathological features was performed through COX multivariate and univariate analysis.The effect of circRNA_001896 downregulation on CC cell propagation was examined using the cell counting kit-8(CCK-8)test,clonogenic,3D sphere formation,and in vivo tumorigenesis assays.Results:Intersection of the GSE113696 and GSE102686 datasets revealed an increased expression of four circRNAs,including circRNA_001896,in CC tissues.Fluorescence quantitative PCR confirmed circRNA_001896 as a circular RNA.High expression of circRNA_001896 was considerably associated with lymph node metastasis,International Federation of Gynecologists and Obstetricians(FIGO)stage,tumor diameter,and survival period in CC patients.Proportional hazards model(COX)univariate and multivariate analyses revealed that circRNA_001896 expressions are a distinct risk factor affecting CC patients’prognosis.Cellular functional experiments showed that downregulating circRNA_001896 substantially suppressed CC cell growth,colony formation,and 3D sphere-forming ability.In vivo,tumorigenesis analysis in nude mice demonstrated that downregulating circRNA_001896 remarkably reduced the in vivo proliferation capacity of CC cells.Conclusion:CircRNA_001896 is highly expressed in CC tissues and is substantially related to lymph node metastasis,FIGO stage,tumor size,and survival period in patients.Moreover,downregulating circRNA_001896 significantly inhibits both in vivo and in vitro propagation of CC cells.Therefore,circRNA_001896 might be used as a biomarker for targeted therapy in cervical cancer.

Knockdown Annexin A8 inhibits the proliferation and invasion of cervical cancer cells

This study aimed to explore the expression,function,and molecular mechanism of ANXA8,the gene for annexin 8,in cervical cancer.Methods:The gene expression of the ANX family members in cervical cancer tissues was classified via The Cancer Genome Atlas(TCGA)database.The expression of ANXA8 in paracancerous tissues,cervical cancer tissues,and cell lines was identified by fluorescence quantitative polymerase chain reaction(PCR)and immunohistochemistry.The effects of ANXA8 knockdown on the cellular growth and cell invasion of cervical cancer were examined by MTT,clone-formation assay,scratch test,and Transwell assay.The effect of ANXA8 knockdown on the proliferative potency of cervical cancer cells was assessed through an in vivo nude-mouse tumor-formation test.The gene expression levels of Ki-67 and ANXA8 in tumor-bearing tissues were measured through Immunohistochemical analysis.Results:TCGA data analysis and fluorescence quantitative PCR exhibited significantly increased expression of ANXA8 in cervical cancer tissues.Further examination exhibited that ANXA8 was expressed exceedingly in cervical cancer tissue,and it was associated with lymph node metastasis,FIGO stage,degree of differentiation,and infiltration depth of cancer patients.Cell-function assays revealed that knocking down ANXA8 may substantially suppress the propagation,colony formation,invasion,and migration of cervical cancer cells.In vivo experiments demonstrated that ANXA8 knockdown had a substantial inhibitory effect on the propagation of cervical cancer cells in nude mice and inhibited the expression of Ki-67.Conclusion:ANXA8 is specifically and significantly upregulated within cervical cancer tissues.The knockdown of this gene showed remarkable outcomes,as evidenced by the substantial inhibition of cervical cancer cell proliferation in in vitro and in vivo experimentations.Therefore,ANXA8 is a potential target or a marker that can serve as a therapeutic or diagnostic tool for cervical cancer.

Role of cyclic guanosine monophosphate-adenosine monophosphate synthase-stimulator of interferon genes pathway in diabetes and its complications

Diabetes mellitus(DM)is one of the major causes of mortality worldwide,with inflammation being an important factor in its onset and development.This review summarizes the specific mechanisms of the cyclic guanosine monophosphate-adenosine monophosphate synthase(cGAS)-stimulator of interferon genes(STING)pathway in mediating inflammatory responses.Furthermore,it compre-hensively presents related research progress and the subsequent involvement of this pathway in the pathogenesis of early-stage DM,diabetic gastroenteropathy,diabetic cardiomyopathy,non-alcoholic fatty liver disease,and other complic-ations.Additionally,the role of cGAS-STING in autonomic dysfunction and intes-tinal dysregulation,which can lead to digestive complications,has been discuss-ed.Altogether,this study provides a comprehensive analysis of the research advances regarding the cGAS-STING pathway-targeted therapeutic agents and the prospects for their application in the precision treatment of DM.

Sleep as a window to understand and regulate Alzheimer's disease:emerging roles of thalamic reticular nucleus

Introduction:Alzheimer 's disease(AD) is a common neurodegenerative disorder and the primary cause of dementia. Considerable evidence supports the “amyloid hypothesis,” stating that the pathogenesis of AD is primarily caused by the deposition of amyloid-β(Aβ), which drives tau phosphorylation, neuroinflammation, and neurodegeneration in the brain. The amyloid hypothesis is strengthened by the significant and moderate benefit of lecanemab, a humanized antibody through an anti-amyloid mechanism,showing slowed clinical decline(van Dyck et al.,2023). The recent positive results of anti-amyloid trials have brought back focus on the amyloid hypothesis through biochemical, genetic, and pharmacological approaches(Zhang, 2023).

A new species in the genus Diaonidia Takahashi(Hemiptera:Coccomorpha:Diaspididae)from China

A new armoured scale,Diaonidia litsea sp.nov.,is described and illustrated.It was found on Litsea monopetala(Roxb.)Pers.in Hainan,China.This new species can be easily distinguished by:the anterior spiracles each with 2–3 discoidal glands;pygidium with 2 pairs of well-developed lobes,and the area around mouthpart strongly sclerotized.An identification key to the adult females in the genus Diaonidia is also provided.

Full-duplex prototype based on joint passive and digital cancellation method

Recent research shows that it is possible to achieve the full-duplex system by cancelling strong self-interference signals, which can be divided into three classes respectively, i.e., passive cancellation, active cancellation and digital cancellation. This pa- per tries to achieve the full-duplex system without using active cancellation, thus a full-duplex system using a joint mechanism based on a novel passive cancellation method and a novel digital cancellation method is proposed. Therein, a good antenna place- ment guided by the theory of the antenna electromagnetic field for the passive cancellation is presented. For the proposed digital can- cellation method, unlike previous separate mechanisms, it is de- signed by using the recursive least square （RLS） algorithm jointly with passive cancellation. The self-interference channel state in- formation （CSI） is transferred as the input of digital cancellation to balance the performance and the complexity. Experimental results show that the proposed self-interference cancellation mechanism can achieve about 85 dB which is better than the previous re- search. Meanwhile, this design provides a better performance compared with half-duplex with both line-of-sight channel and non- line-of-sight channel.

Discovery of Kaempferol,a Novel ADAM10 Inhibitor,as a Potential Treatment for Staphylococcus aureus Infection

Host-directed therapy(HDT)is an emerging novel approach for treating multidrug-resistant Staphylococcus aureus(S.aureus)infection.Functioning as the indispensable specific cellular receptor for a-toxin(Hla),a-disintegrin and metalloproteinase 10(ADAM10)is exploited to accelerate S.aureus infection through diverse mechanisms.The extraordinary contribution of ADAM10 to S.aureus pathogenesis renders it an attractive HDT target for combating S.aureus infection.Our study is the first to demonstrate the indispensable role of ADAM10 in S.aureus-induced necroptosis,and it enhances our knowledge of the role of ADAM10 in S.aureus infection.Using a fluorogenic substrate assay,we further identified kaempferol as a potent ADAM10 inhibitor that effectively protected mice from S.aureus infection by suppressing Hla-mediated barrier disruption and necroptosis.Collectively,our work presents a novel hostdirected therapeutic strategy for using the promising candidate kaempferol to treat S.aureus infection and other diseases relevant to the disordered upregulation of ADAM10.

Effects of diquafosol sodium in povidone iodine-induced dry eye model

AIM:To investigate the effects of diquafosol sodium(DQS)for dry eye model induced with povidone-iodine(PI)solution.METHODS:Ten Sprague Dawley rats as the control group.Thirty Sprague Dawley rats were used to establish the dry eye model with stimulation of 10 g/L PI for 14d,then divided rats into three groups:dry eye group with no treatment(DED group,n=10);phosphate buffer saline treated group(PBS group,n=10);diquafosol treated group(DQS group,n=10).Clinical changes were observed by tear production test,fluorescein staining,tear breakup time(TBUT)test,corneal confocal microscope and ocular surface comprehensive analyzer.Eyeballs were collected on day 10 of treatment for hematoxylin-eosin(HE),periodic acid-Schiff(PAS),and alcian blue staining.TUNEL assay,polymorphonuclear(PMN)and mucin 1(MUC1)immunofluorescence were performed and corneal ultrastructural changes were detected by electron microscopy.RESULTS:Compared with DED and PBS groups,tear production(7.26±0.440 vs 4.07±0.474 mm;7.26±0.440 vs 3.74±0.280 mm;all P<0.01)and TBUT(7.37±0.383s vs 1.49±0.260s;7.37±0.383s vs 1.42±0.437s;all P<0.01)were significantly increased in DQS group.HE,PAS,and alcian blue staining and MUC1 immunofluorescence showed mucins and conjunctival goblet cells density(8.45±0.718 vs 5.21±0.813 cells/0.1 mm^(2);8.45±0.718 vs 5.36±0.615 cells/0.1 mm^(2);all P<0.01)increased in DQS group.Confocal microscopy,PMN immunofluorescence and TUNEL staining showed inflammatory infiltration and corneal epithelial cells apoptosis decreased in DQS group.The increased number of microvilli in corneal epithelial and the recovered cell junction were observed in DQS group.CONCLUSION:PI instillation can induce goblet cells and mucin loss,epithelial cell apoptosis and inflammation,which are consistent with the pathological manifestations of dry eye.Diquafosol can repair the ocular surface damage caused by PI,reduce corneal inflammation,inhibit corneal epithelial cell apoptosis,promote mucin secretion and maintain tear film stability.

Atomic Valley Filter Effect Induced by an Individual Flower Defect in Graphene

Owing to the bipartite nature of honeycomb lattice,the electrons in graphene host valley degree of freedom,which gives rise to a rich set of unique physical phenomena including chiral tunneling,Klein paradox,and quantum Hall ferromagnetism.Atomic defects in graphene can efficiently break the local sublattice symmetry,and hence,have significant effects on the valley-based electronic behaviors.Here we demonstrate that an individual flower defect in graphene has the ability of valley filter at the atomic scale.With the combination of scanning tunneling microscopy and Landau level measurements,we observe two valley-polarized density-of-states peaks near the outside of the flower defects,implying the symmetry breaking of the K and K′valleys in graphene.Moreover,the electrons in the K valley can highly penetrate inside the flower defects.In contrast,the electrons in the K′valley cannot directly penetrate,instead,they should be assisted by the valley switch from the K′to K.Our results demonstrate that an individual flower defect in graphene can be regarded as a nanoscale valley filter,providing insight into the practical valleytronics.

Integrative transcriptome analysis identifies MYBL2 as a poor prognosis marker for osteosarcoma and a pan-cancer marker of immune infiltration

MYBL2(MYB proto-oncogene like 2)is an emerging prognostic marker for malignant tumors,and its potential role in osteosarcoma and its relationship with immune infiltration in pan-cancer is yet to be elucidated.We constructed a transcription factor activity profile of os-teosarcoma using the single-cell regulatory network inference algorithm based on single-cell RNA sequencing data obtained from the Gene Expression Omnibus.Subsequently,we calcu-lated the extent of MYBL2 activation in malignant proliferative osteoblasts.We also explored the association between MYBL2 and chemotherapy resistance in osteosarcoma.Furthermore,we systematically correlated MYBL2 with immunological signatures in the tumor microenviron-ment in pan-cancer,including immune cell infiltration,immune checkpoints,and tumor immu-notherapy prognosis.Finally,we developed and validated a risk score(MRGS),derived an osteosarcoma risk score nomogram based on MRGS,and tested its ability to predict prognosis.MYBL2 and gene enrichment analyses in osteosarcoma and pan-cancer revealed that MYBL2 was positively correlated with cell proliferation and tumor immune pathways.MYBL2 expres-sion positively correlated with SLC19A1 in pan-cancer and osteosarcoma cell lines.Pan-cancer immune infiltration analysis revealed that MYBL2 was correlated with myeloid-derived sup-pressor cells,Th2 cell infiltration,CD276,RELT gene expression,and tumor mutation burden.

3D microstructures of nuclear graphite:IG-110,NBG-18 and NG-CT-10

Molten salt is used as primary coolant flowing through graphite moderator channel of a molten salt reactor.Working at high temperature under radiation environment,the pore network structure of nuclear graphite should be well understood.In this paper,X-ray tomography is employed to study the 3D pore structure characteristics of nuclear grades graphite of IG-110,NBG-18 and NG-CT-10,and permeability simulation through geometries are performed.The porosity,number of pores and throats,coordination number and pore surface are obtained.NGCT-10 is of similar microstructure to IG-110,but differs significantly from NBG-18.The absolute permeabilities of IG-110,NG-CT-10 and NBG-18 are 0.064,0.090 and0.106 mD,respectively.This study provides basis for future research on graphite infiltration experiment.

“内外联动,三点一线”,助力复养工作

中国是世界养猪大国,同时也是全世界第一猪肉消费大国。非洲猪瘟的暴发给中国的生猪养殖行业带来了巨大冲击。生猪存栏数量下降,散户大部分退出,使得市场上的猪肉供给受到一定程度的影响。依靠从国外进口猪肉来解决国内的消费需求能够起到一定的缓冲作用。但从长远考虑,如何防治非洲猪瘟,抓住“复养”环节所需,才是治本之道。文章借鉴已有研究成果,从政策背景出发,对复养环节的问题和注意事项进行论述,结合四川省和江苏省的成功案例,旨在为生猪养殖业的复养工作提供参考和建议。

减抗、限抗对生猪养殖行业的影响分析

2018年4月20日,农业农村部出台了《兽用抗菌药使用减量化行动试点工作方案(2018-2021年)》(以下简称《方案》)。从国家层面来看,《方案》旨在通过推进养殖业的绿色发展进程,从而进一步实现振兴乡村这一伟大目标。对于畜牧业而言,国家的此项举措无疑是为了促进畜牧行业的长远发展,但是在实施过程中,势必会与当下的养殖现状产生一些冲突。对于这些冲突,我们需要分析其成因及影响,从而为保证方案的顺利实施提供可供参考的措施。文章以生猪养殖为例,结合《方案》出台的背景,从可能出现的问题以及应对措施等方面进行分析、论述,从而为养猪人应对减抗、限抗提供参考依据。

TGF-β1受体ALK1和ALK5在翼状胬肉和正常结膜组织中的差异表达

目的:研究不同种类的转化生长因子β-1(TGF-β1)激活素受体样激酶(ALK)在翼状胬肉和正常结膜组织中的表达及其意义。方法:前瞻性研究。选取2020-06/12在徐州医科大学附属医院眼科就诊的40例手术中切除的翼状胬肉标本和40例白内障手术获取的正常结膜标本纳入本次研究。使用ALK1、ALK5免疫组织化学染色观察TGF-β1受体在胬肉和正常结膜组织中的表达定位,并统计染色阳性细胞比例;使用RT-PCR检测ALK1、ALK5 mRNA在胬肉和结膜组织中的表达;并以Western Blot检测ALK1、ALK5蛋白的表达。结果:根据免疫组织化学染色结果,ALK1在翼状胬肉组中的表达水平较正常结膜组明显升高,并且在整个翼状胬肉上皮细胞中均有表达,而在正常结膜组织中仅在上皮细胞的基底层中有表达;两组上皮细胞基底层均检测到ALK5,而翼状胬肉组ALK5水平较正常结膜组下降。两组间ALK1、ALK5阳性细胞比例有显著的差异(均P<0.05)。RT-PCR结果显示,与结膜组织相比,ALK1 mRNA在翼状胬肉中的表达明显增强,ALK5的表达明显减弱,两组间有显著差异(均P<0.05),Western Blot结果显示ALK1、ALK5蛋白的表达差异与RT-PCR结果基本一致。结论:翼状胬肉和正常结膜ALK的表达谱有明显的差异。与正常结膜组织相比,翼状胬肉组织的ALK1表达增强,ALK5表达减弱,表明TGF-β信号通路处于不同的活化状态,对于进一步研究翼状胬肉的发病机制提供实验依据。

Behaviors of fine(IG-110)and ultra-fine(HPG-510)grain graphite irradiated by 7 MeV Xe^26+ions

Developing a molten salt reactor needs molten salt-impermeable nuclear graphite. Ultra-fine grain graphite is a good choice as it is better in permeability than fine grain graphite. In this paper, ultra-fine grain graphite(HPG-510) and fine grain graphite(IG-110) samples are irradiated at room temperature by 7 MeV Xe ions to doses of 1 × 10^(14)-5 × 10^(15) ions/cm^2. Scanning electron microscopy, transmission electron microscopy(TEM), Raman spectroscopy and nano-indentation are used to study the radiation-induced changes. After irradiation of different doses, all the HPG-510 samples show less surface fragment than the IG-110 samples. The TEM and Raman spectra,and the hardness and modulus characterized by nano-indentation, also indicate that HPG-510 is more resistant to irradiation.

Reliability of Chinese web-based ocular surface disease index questionnaire in dry eye patients:a randomized,crossover study

AIM:To assess the reliability of web-based version of ocular surface disease index in Chinese(C-OSDI)on clinically diagnosed dry eye disease(DE)patients.METHODS:A total of 254 Chinese participants(51%male,129/254;mean age:27.90±9.06 y)with DED completed paper-and web-based versions of C-OSDI questionnaires in a randomized crossover design.Ophthalmology examination and DED diagnosis were performed prior to the participants being invited to join the study.Participants were randomly designated to either group A(paper-based first and webbased second)or group B(web-based first and paper-based second).Final data analysis included participants that had successfully completed both versions of the C-OSDI.Demographic characteristics,test-retest reliability,and agreement of individual items,subscales,and total score were evaluated with intraclass correlation coefficients(ICC),Spearman rank correlation,Wilcoxon test and Rasch analysis.RESULTS:Reliability indexes were adequate,Pearson correlation was greater than 0.8 and ICCs range was 0.827 to 0.982;total C-OSDI score was not statistically different between the two versions.The values of mean-squares fit statistics were very low compared to 1,indicating that the responses to the items by the model had a high degree of predictability.While comparing the favorability 72%(182/254)of the participants preferred web-based assessment.CONCLUSION:Web-based C-OSDI is reliable in assessing DED and correlation with the paper-based version is significant in all subscales and overall total score.Webbased C-OSDI can be administered to assess individuals with DED as participants predominantly favored online assessment.

Epiphyseal distraction and hybrid reconstruction using polymethyl methacrylate construct combined with free non-vascularized fibular graft in pediatric patients with osteosarcoma around knee: A case report

BACKGROUND In children with osteosarcoma around the knee joint without epiphysis involvement,joint-sparing surgery seems to be an ideal way to retain knee joint function.However,there are two points of debate with regard to the technique:How to accurately achieve a safe surgical margin,and how to achieve intercalary reconstruction of the massive bone defect following resection of the tumor.CASE SUMMARY We present the case of an 8-year-old girl with osteosarcoma of the distal femur without involvement of the epiphysis.Epiphyseal distraction was applied to separate the epiphysis and metaphysis,and this provided a safe surgical margin.The massive bone defect was reconstructed with a custom-made antibioticloaded polymethyl methacrylate(PMMA)construct combined with a free nonvascularized fibular graft.Six months after surgery,bone union between the autograft and host bone was confirmed in both the proximal and distal femur by computer tomography(CT)examination.Moreover,considerable callus formation was found around the PMMA construct.After 28 mo of follow-up,there was no sign of recurrence or metastasis.The patient could walk without any aid and carry out her daily life activities satisfactorily.CONCLUSION In cases of osteosarcoma without epiphysis involvement,epiphyseal distraction can be easily applied to obtain a safe margin.Hybrid reconstruction with an antibiotic-loaded PMMA construct combined with a free non-vascularized fibular graft has the advantages of being easy to manufacture,less time-consuming to place,and less likely to get infected,while also ensuring bone union.Our case provides an alternative technique for biological reconstruction after joint-sparing surgery in patients with osteosarcoma around the knee without epiphyseal involvement.

Power Quality Assessment Based on Rough AHP and Extension Analysis

Due to the increasing power consumption of whole society and widely using of new non-linear and asymmetric electrical equipment,power quality assessment problem in the new period has attracted more and more attention.The mathematical essence of comprehensive assessment of power quality is a multiattribute optimal decision-making problem.In order to solve the key problem of determining the indicator weight in the process of power quality assessment,a rough analytic hierarchy process(AHP)is proposed to aggregate the judgment opinions of multiple experts and eliminate the subjective effects of single expert judgment.Based on the advantage of extension analysis for solving the incompatibility problem,extension analysis method is adopted to assess the power quality.The assessment grades of both total power quality and each assessment indicator are obtained by correlation function.Through a case of 110 kV bus of a converting station in a wind farm of China,the feasibility and effectiveness of the propose method are demonstrated.The result shows that the proposed method can determine the overall power quality of power grid,as well as compare the differences among the performance of assessment indicators and provide the basis for further improving of power quality.

A Weighted Combination Forecasting Model for Power Load Based on Forecasting Model Selection and Fuzzy Scale Joint Evaluation

To solve the medium and long term power load forecasting problem,the combination forecasting method is further expanded and a weighted combination forecasting model for power load is put forward.This model is divided into two stages which are forecasting model selection and weighted combination forecasting.Based on Markov chain conversion and cloud model,the forecasting model selection is implanted and several outstanding models are selected for the combination forecasting.For the weighted combination forecasting,a fuzzy scale joint evaluation method is proposed to determine the weight of selected forecasting model.The percentage error and mean absolute percentage error of weighted combination forecasting result of the power consumption in a certain area of China are 0.7439%and 0.3198%,respectively,while the maximum values of these two indexes of single forecasting models are 5.2278%and 1.9497%.It shows that the forecasting indexes of proposed model are improved significantly compared with the single forecasting models.

A Wireless Intelligent Business Laundry Service System

For the business washing machine industry, most are currently using traditional manual service (the way cost is high, inefficient, low degree of intelligence) of the status quo. The wireless intelligent business laundry service system based on ESP8266 communication module is developed, which has the Wechat payment function and realizes the automatic control service of the washing machine. Firstly, the laundry wireless terminal will send a laundry request to the laundry control center when the customer is ready to wash. Secondly, after receiving the laundry request, control center will send WeChat Public Accounts, idle washing machine number and charges, as well as laundry service time and other information back to the wireless terminal to generate payment QR code. And then the customer scans QR code, when the control center receives the information that payment is successful, the system will automatically control the washing machine to complete the laundry service work. After testing, the system has the advantages of accurate control, low energy consumption, strong stability, accurate data transmission, low cost, strong practicability, and is conducive to promoting the development of the business laundry industry.