Wampee(Clausena lansium)is an important evergreen fruit tree native to southern China that has a long history of use for medicinal purposes.Here,a chromosome-level genome of C.lansium was constructed with a genome siz...Wampee(Clausena lansium)is an important evergreen fruit tree native to southern China that has a long history of use for medicinal purposes.Here,a chromosome-level genome of C.lansium was constructed with a genome size of 282.9 Mb and scaffold N50 of 30.75 Mb.The assembled genome contains 48.70%repetitive elements and 24,381 protein-coding genes.Comparative genomic analysis showed that C.lansium diverged from Aurantioideae 15.91-24.95 million years ago.Additionally,some expansive and specific gene families related to methyltransferase activity and S-adenosylmethionine-dependent methyltransferase activity were also identified.Further analysis indicated that N-methyltransferase(NMT)is mainly involved in alkaloid biosynthesis and O-methyltransferase(OMT)participates in the regulation of coumarin accumulation in wampee.This suggested that wampee's richness in alkaloids and coumarins might be due to the gene expansions of NMT and OMT.The tandem repeat event was one of the major reasons for the NMT expansion.Hence,the reference genome of C.lansium will facilitate the identification of some useful medicinal compounds from wampee resources and reveal their biosynthetic pathways.展开更多
[ Objective] This study aimed to investigate the ehangos of aroma components in mango fruit at different post-harvest stages. [ Method] The aroma com- ponents in ＇ Keitt＇ mango fruit at 3,6, 9 and 13 d postharvest w...[ Objective] This study aimed to investigate the ehangos of aroma components in mango fruit at different post-harvest stages. [ Method] The aroma com- ponents in ＇ Keitt＇ mango fruit at 3,6, 9 and 13 d postharvest were extracted using heM-space solid phase mieroextraction （HS-SPME） and analyzed by gas chre- matograph-rnass speetrephotometer （GC-MS）. [ Result] The results showed that a total of 42 aroma compounds were identified from the mango samples at four sta- ges. To be specific, monoterpene and sesquiterpene were the major components; at 3, 6, 9 and 13 d postharvest, the relative contents of terpene were 94.24%, 98.91%, 55.32% and 95.97%, respectively. [ Conclusion] IS-（ ＋ ）-3-earene is the characteristic aroma component in ripe fruit of ＇ Keitt＇ mango. The aroma of mango fruit is also affected by ester, alcohol and aldehyde compounds.展开更多
基金supported by the Central Public-interest Scientific Institution Basal Research Fund for the Chinese Academy of Tropical Agricultural Sciences(1630062019010 and 1630062020010)the Fund of Protection of Species Resources for the Ministry of Agriculture and Rural Affairs of China(125A0605)。
文摘Wampee(Clausena lansium)is an important evergreen fruit tree native to southern China that has a long history of use for medicinal purposes.Here,a chromosome-level genome of C.lansium was constructed with a genome size of 282.9 Mb and scaffold N50 of 30.75 Mb.The assembled genome contains 48.70%repetitive elements and 24,381 protein-coding genes.Comparative genomic analysis showed that C.lansium diverged from Aurantioideae 15.91-24.95 million years ago.Additionally,some expansive and specific gene families related to methyltransferase activity and S-adenosylmethionine-dependent methyltransferase activity were also identified.Further analysis indicated that N-methyltransferase(NMT)is mainly involved in alkaloid biosynthesis and O-methyltransferase(OMT)participates in the regulation of coumarin accumulation in wampee.This suggested that wampee's richness in alkaloids and coumarins might be due to the gene expansions of NMT and OMT.The tandem repeat event was one of the major reasons for the NMT expansion.Hence,the reference genome of C.lansium will facilitate the identification of some useful medicinal compounds from wampee resources and reveal their biosynthetic pathways.
基金Supported by Central Nonprofit Basic Scientific Research Project for the Scientific Research Institutes of China(No.1630062012003)
文摘[ Objective] This study aimed to investigate the ehangos of aroma components in mango fruit at different post-harvest stages. [ Method] The aroma com- ponents in ＇ Keitt＇ mango fruit at 3,6, 9 and 13 d postharvest were extracted using heM-space solid phase mieroextraction （HS-SPME） and analyzed by gas chre- matograph-rnass speetrephotometer （GC-MS）. [ Result] The results showed that a total of 42 aroma compounds were identified from the mango samples at four sta- ges. To be specific, monoterpene and sesquiterpene were the major components; at 3, 6, 9 and 13 d postharvest, the relative contents of terpene were 94.24%, 98.91%, 55.32% and 95.97%, respectively. [ Conclusion] IS-（ ＋ ）-3-earene is the characteristic aroma component in ripe fruit of ＇ Keitt＇ mango. The aroma of mango fruit is also affected by ester, alcohol and aldehyde compounds.
