Clinical Effect of Yinhuang Qingfei Capsules in Treatment of Asymptomatic and Mild/Common Severe Acute Respiratory Syndrome Coronavirus 2 Infection:An Analysis of 242 Cases

[Objectives]To investigate the clinical effect of Yinhuang Qingfei capsules in the treatment of asymptomatic and mild/common severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)infection.[Methods]A total of 362 patients with SARS-CoV-2 infection were divided into the treatment group with 242 patients and control group with 120 patients according to their treatment regimen.The patients in the control group were given standard treatment regimen and those in the treatment group were given Yinhuang Qingfei capsules in addition to the treatment in the control group.The two groups were observed in terms of average length of hospital stay,mean time for nucleic acid clearance,TCM syndrome score,and progression to severe/critical illness,and clinical outcome was compared between the two groups.[Results]There was a significant difference in the overall response rate between the treatment group and the control group[97.52%(236/242)vs 95.00%(114/120),P<0.05].Compared with the control group,the treatment group had significantly shorter length of hospital stay and time for nucleic acid clearance(P<0.05).After 7 days of treatment,both groups had a significant change in TCM syndrome score,and there was a significant difference in TCM syndrome score between the two groups(P<0.05);after 15 days of treatment,both groups had a TCM syndrome score of 0.Progression to severe/critical illness was not observed in either group.[Conclusions]Compared with the standard treatment regimen alone,standard treatment regimen combined with Yinhuang Qingfei capsules can effectively shorten the length of hospital stay and time for nucleic acid clearance and improve TCM symptoms in patients with asymptomatic and mild/common SARS-CoV-2 infection.

Assisting Bi_(2)MoO_(6) microspheres with phenolic resin-based ACSs as attractive tailor-made supporter for highly-efficient photocatalytic CO_(2) reduction

It is rather essential to design glorious system with high CO_(2) adsorption capacity and electron migration efficiency for improving selective and effective CO_(2) reduction into solar fuels.Here,as-synthesized phenolic resin spheres via suspension polymerization were carbonized and activated by water vapor to obtain activated carbon spheres(ACSs).Subsequently,Bi_(2)MoO_(6)/ACSs were prepared via hydrothermal-impregnated method.The systematical characterizations of samples,including XRD,XPS,SEM,EDX,DRS,BET,PL,CO_(2) adsorption isotherm,EIS and transient photocurrent,were analyzed.The results clearly demonstrated that Bi_(2)MoO_(6) with suitable oxidation reduction potentials and bandgap and ACSs with admirable CO_(2) adsorption and electrical conductivity not only enhanced separation efficiency of photoindued electron-hole pair,but also displayed as 1.8 times CO_(2) reduction activity to CO as single Bi_(2)MoO_(6) sample under Xe-lamp irradiation.Finally,a concerned photocatalytic CO_(2) reduction mechanism was proposed and investigated.Our findings should provide innovative guidance for designing a series of photocatalytic CO_(2) reduction materials with highly efficient and selective ability.

Supplemental N-acyl homoserine lactonase alleviates intestinal disruption and improves gut microbiota in broilers challenged by Salmonella Typhimurium

Background Salmonella Typhimurium challenge causes a huge detriment to chicken production.N-acyl homoserine lactonase(AHLase),a quorum quenching enzyme,potentially inhibits the growth and virulence of Gram-negative bacteria.However,it is unknown whether AHLase can protect chickens against S.Typhimurium challenge.This study aimed to evaluate the effects of AHLase on growth performance and intestinal health in broilers challenged by S.Typhimurium.A total of 240 one-day-old female crossbred broilers(817C)were randomly divided into 5 groups(6 replicates/group):negative control(NC),positive control(PC),and PC group supplemented with 5,10 or 20 U/g AHLase.All birds except those in NC were challenged with S.Typhimurium from 7 to 9 days of age.All parameters related to growth and intestinal health were determined on d 10 and 14.Results The reductions(P<0.05)in body weight(BW)and average daily gain(ADG)in challenged birds were alleviated by AHLase addition especially at 10 U/g.Thus,samples from NC,PC and PC plus 10 U/g AHLase group were selected for further analysis.S.Typhimurium challenge impaired(P<0.05)intestinal morphology,elevated(P<0.05)ileal inflammatory cytokines(IL-1βand IL-8)expression,and increased(P<0.05)serum diamine oxidase(DAO)activity on d 10.However,AHLase addition normalized these changes.Gut microbiota analysis on d 10 showed that AHLase reversed the reductions(P<0.05)in several beneficial bacteria(e.g.Bacilli,Bacillales and Lactobacillales),along with increases(P<0.05)in certain harmful bacteria(e.g.Proteobacteria,Gammaproteobacteria,Enterobacteriaceae and Escherichia/Shigel a)in PC group.Furthermore,AHLase-induced increased beneficial bacteria and decreased harmful bacteria were basically negatively correlated(P<0.05)with the reductions of ileal IL-1βand IL-8 expression and serum DAO activity,but positively correlated(P<0.05)with the increased BW and ADG.Functional prediction revealed that AHLase abolished S.Typhimurium-induced upregulations(P<0.05)of certain pathogenicity-related pathways such as lipopolysaccharide biosynthesis,shigellosis,bacterial invasion of epithelial cells and pathogenic Escherichia coli infection of gut microbiota.Conclusions Supplemental AHLase attenuated S.Typhimurium-induced growth retardation and intestinal disruption in broilers,which could be associated with the observed recovery of gut microbiota dysbiosis.

地铁出入段线复杂基坑支护优化对基坑变形的影响

为了探究复杂地质条件下基坑支护优化后对基坑整体的影响,结合经济成本和施工工期等因素,将部分基坑支护结构中SMW工法桩优化为拉森钢板桩。通过电子水平仪和测斜仪对基坑的水平位移和地表沉降量进行监测,结果表明:在复杂地质条件下,基坑支护的优化可以大量节省施工时间以及工程成本,基坑支护优化后的基坑监测结果均满足设计要求,达到较为理想的效果。基坑支护优化后的沉降量改变不是很大,地表沉降量和地表沉降平均速率略有增加,但是单月沉降量最大值有所减小;基坑支护优化对于基坑整体的水平位移影响较大,增加了水平位移的总量以及单日水平位移速率。基坑支护形式改变对于钢支撑的轴力变化影响较大,但依旧处于安全范围内。

Determination of Compositions in Cosmetics by Multiple-Instrument

An analysis method based on multi-instruments analysis technique coupled solvent extraction to determine compositions of unknown cosmetic were established. The multi-instruments involve high-performance liquid chromatography (HPLC), X-ray diffraction (XRD) and Fourier transform infrared (FTIR). The cosmetic sample was separated and enriched into 4 fractions by the solvent extraction. The compounds were defined as mercuric ammonium chloride, liquid paraffin, etc.

Determination of Sedative Component in Chinese Medicines by High Resolution Chromatography-Electron Impact-Mass Spectrometry

The sedative compounds in two traditional Chinese medicines were studied. These compounds are present in the medicinal herbs “Zao Ren” and “Lu Lu Tong”. The samples were first extracted by ethanol and pre-treated by ultrasonic wave, and then they were separated and qualitatively analyzed by high-resolution GC-EI-MS and High Performance Liquid Chromatography (HPLC) systems. We have found that the Chinese herb medicines “Zao Ren” contained clonazepam (120 ppm), whereas “Lu Lu Tong” Chinese drug contained estazolam (270 ppm). The Analytical measurements were done by using three different diagnostic indices using the GC-retention time of the GC-EI-MS total –ion–chromatogram;single stage EI-MS analysis identification and from the measurements of the retention time in the HPLC.

N-acyl homoserine lactonase attenuates the virulence of Salmonella typhimurium and its induction of intestinal damages in broilers

This study aimed to investigate the potential mitigating effects of N-acyl homoserine lactonase(AHLase)on the virulence of Salmonella typhimurium and its induction of intestinal damages in broilers.In vitro study was firstly conducted to examine if AHLase treatment could attenuate the virulence of S.typhimurium.Then,an in vivo experiment was performed by allocating 240 broiler chicks at 1 d old into 3 groups(8 replicates per group):negative control(NC),positive control(PC),and PC supplemented with 10,000 U/kg AHLase.All chicks except those in NC were orally challenged by S.typhimurium from 8to 10 d of age.Parameters were measured on d 11 and 21.The results showed that treatment with 1 U/mL AHLase suppressed the biofilm-forming ability(including biofilm biomass,extracellular DNA secretion and biofilm formation-related gene expression),together with swarming motility and adhesive capacity of S.typhimurium.Supplemental 10,000 U/kg AHLase counteracted S.typhimurium-induced impairments(P<0.05)in broiler growth performance(including final body weight,average daily gain and average daily feed intake)during either 1-11 d or 12-21 d,and increases(P<0.05)in the indexes of liver,spleen and bursa of Fabricius on d 11,together with reductions(P<0.05)in ileal villus height and its ratio to crypt depth on both d 11 and 21.AHLase addition also normalized the increased(P<0.05)m RNA expression of ileal occludin on both d 11 and 21 in S.typhimurium-challenged broilers.However,neither S.typhimurium challenge nor AHLase addition altered(P>0.05)serum diamine oxidase activity of broilers.Noticeably,S.typhimurium challenge caused little change in the mRNA expression of ileal inflammatory cytokines except for an increase(P<0.05)in interleukin-8 expression on d 11,whereas AHLase addition normalized(P<0.05)this change.In conclusion,AHLase treatment could attenuate the virulence and pathogenicity of S.typhimurium,thus contributing to alleviate S.typhimurium-induced growth retardation and intestinal damages in broilers.

Research on digital transformation of China's traditional manufacturing industry enabled by digital economy based on system dynamics

The digital transformation of traditional manufacturing industry is an inevitable trend and inherent development requirement of China’s digital economy.This paper systematically researches the digital transformation mechanism of traditional manufacturing industry enabled by digital economy from the social,technical,innovative environmental and organizational sub-systems based on the system dynamics(SD)and the social shaping of technology theory(SST).The simulated experiments predicate the different digital transformation paths of the labor-intensive,capital-intensive and technology-intensive traditional manufacturing enterprises under the guidance of Industry 4.0 or"Industry of the future"policy by constructing the system dynamics model of causality,stock flow process,which has vital significance for China’s manufacturing industry to transform from a giant towards a manufacturing power.