用于检测痕量铅离子的功能化反射结构光纤干涉传感器

铅离子(Pb^(2+))是日常生活中常接触的有毒重金属污染物之一。本研究开发了一种新型反射式光纤干涉传感器,用于检测痕量铅离子。该传感器结构由单模光纤、无芯光纤和细芯光纤(TCF)依次拼接而成。TCF的包层被氢氟酸部分腐蚀并涂覆功能化的水凝胶传感膜。该传感膜选用甲基丙烯酸2-羟基乙酯(2-HEMA)作为识别单体。2-HEMA中的氧原子能与Pb^(2+)发生配体-受体相互作用,形成“-O-Pb-O-”交联结构,从而改变TCF的新包层有效折射率。因此,可以通过观察反射光谱中光信号的变化来检测水溶液中Pb^(2+)的浓度。所提出的传感器具有很高的检测灵敏度(1.926×10^(9)nm·mol^(-1)·L),其检测极限为4.14 ppt(1 ng·L^(-1)=1 ppt),比世界卫生组织(WHO)规定的饮用水中Pb^(2+)(10 ppb,1μg·L^(-1)=1 ppb)浓度低1000倍。此外,利用一个方程组实现了该传感器的温度自校准功能,成功地消除了环境温度的干扰。由于该传感器良好的特异性、稳定性以及反射式结构,非常便于实时远程检测,为环境和人类健康监测提供了广阔的前景。

Contactless vital signs monitoring based on few‐mode and multi‐core fibers

Few-mode and multi-core fibers are proposed and demonstrated for contactless vital signs monitoring in this paper.In-line optical fiber interferometers using few-mode and multi-core fibers are designed and offset splicing is utilized for mode excitation.Extinction ratio and insertion loss are analyzed experimentally under different offset distances.The fabricated in-line interferometers are packaged under the mattress to realize contactless vital signs signals collection.By using filtering techniques,both respiration and heartbeat signals can be recovered successfully,and respiration as well as heartbeat ratio are obtained.Mode excitation and interference are theoretically analyzed in few-mode fiber while curvature sensing experiments using multi-core fiber interferometer are performed to verify its excellent performance on vital signs monitoring.The successful demonstration on contactless vital signs monitoring makes few-mode and multi-core fibers promising candidates for healthcare applications.

Preparation of Titanium-based Polyoxometalatxiue-ionic Liquid Catalysts and Application in the Synthesis of Degradable Polyester

Three kinds of Keggin-type titanium-based polyoxometalate-ionic liquid catalysts were prepared,and the chemical structure,crystal structure and thermal stability of the three catalysts were analyzed and tested by infrared spectrometer,ultraviolet-visible spectrometer,X-ray diffractometer,and thermogravimetric analyzer,respectively.Afterwards,their performance in catalyzing the coupling reaction of ethylene carbonate(EC)and dimethyl succinate(DMSu)to synthesize polyethylene succinate(PES)was studied,and the effects of different metals on the catalytic performance and the mechanism of action were discussed.The optimal process parameters for the coupling reaction were explored.Finally,the chemical structure of PES was characterized by infrared spectrometer.The results show that under the same process parameters,the polyoxometalate-ionic liquid catalyst Bmim_(5)[Ti(H_(2)O)TiMo_(11)O_(39)]had the best catalytic effect.Under the optimal process parameters,Bmim_(5)[Ti(H_(2)O)TiMo_(11)O_(39)]had obvious inhibitory effect on side reactions,and the yield and selectivity of PES prepolymer were up to 72.23%and 88.64%.

肿瘤浸润淋巴细胞在实体肿瘤中作用的研究进展

肿瘤是21世纪威胁人类健康的主要疾患之一。临床上,实体瘤治疗仍以手术切除、放化疗和靶向治疗为主,但这些方法往往不能根除肿瘤病灶,易导致肿瘤复发和进展。肿瘤免疫治疗是利用人体的免疫系统,通过增强或恢复抗肿瘤免疫力实现控制和杀伤肿瘤的一种新的治疗模式。肿瘤免疫治疗能够在众多患者中产生持久反应,过继性免疫治疗和免疫检查点阻断剂治疗均可产生显著的抗原特异性免疫反应。肿瘤浸润淋巴细胞(TILs)是一种存在于肿瘤组织内部具有高度异质性的淋巴细胞,在宿主抗原特异性肿瘤免疫应答中发挥关键作用。最新研究表明,在肿瘤发生和治疗过程中,TILs的亚群组成和数量与患者预后密切相关;抗肿瘤的TILs介导的过继性免疫治疗方法已在多种实体瘤中取得了良好的疗效。文中就实体肿瘤中TILs的研究进展作一综述。

CRISPR/Cas9系统在疾病研究和治疗中的应用

基因组编辑技术(Genomeeditingtechnology)是一种通过人工手段在基因组水平对DNA序列进行改造的遗传操作技术,包括特定DNA片段的插入、敲除、替换和点突变。其中,依赖核酸酶的基因组编辑技术的基本原理是在基因组的特定位置产生双链DNA断裂(Double-strandedbreak,DSB)后通过非同源末端连接(Non-homologous end joining,NHEJ)或同源重组(Homologous recombination,HR)的方式进行修复。随着对核酸酶更深入的研究,基因组编辑技术也得到了快速发展,其中最常使用的核酸酶主要包括巨型核酸酶、锌指核酸酶、转录激活因子样效应物核酸酶以及成簇的规律间隔的短回文重复序列相关蛋白(Clusteredregularly interspaced short palindromic repeats(CRISPR)-associated proteins(Cas)。文中在介绍上述基因组编辑技术的发展及作用原理的基础上,主要综述了CRISPR/Cas9系统在基因功能鉴定、疾病模型建立、基因治疗和免疫治疗等应用领域的研究进展,并对其未来发展进行了展望。

双特异性抗体构建在肿瘤临床治疗中的应用

双特异性抗体(Bispecific antibody,BsAb)是具有两个不同抗原结合位点的抗体,可分为含Fc段和不含Fc段的BsAb,不同结构的BsAb具有不同的特点和应用领域。相比于传统的单克隆抗体,BsAb的灵敏度和特异性更高。更重要的是,BsAb具有募集免疫细胞、双重阻断信号通路等功能,在免疫诊断和治疗中扮演重要角色。随着全球环境的恶化以及人们生活习惯的不规律,肿瘤的发病率越来越高,成为仅次于心脑血管疾病的全球第二大致死疾病,全球每年有1200万新发癌症病例。肿瘤的治疗手段包括手术切除、放化疗和靶向治疗等。肿瘤免疫疗法是近几年新兴的治疗方法,其通过激发自身免疫系统的能力来清除肿瘤细胞。传统的单抗药物虽在肿瘤靶向治疗和免疫治疗中取得了一定的疗效,但肿瘤具有高度的异质性和可塑性,常常引发肿瘤耐药性的出现。双特异性抗体能够同时靶向多个靶点,目前已用于肿瘤的临床治疗,并取得了一定的治疗效果。文中就双特异性抗体在肿瘤临床治疗中的研究进展和应用作一综述。

三维基因组学研究进展

三维基因组学是一门研究基因组三维空间结构与功能的新兴学科,主要研究基因组序列在细胞核内的三维空间构象,及其对DNA复制、DNA重组、基因表达调控等生物过程的生物学效应。自染色质构象捕获技术(3C)出现后,三维基因组学相关研究领域飞速发展。借助于3C及其衍生技术、Hi-C和ChIA-PET等技术,科学家能对各类物种的三维基因组进行更为深入的研究,从而揭示微生物、植物和动物基因组的空间构象、染色质的相互作用模式、转录调控以及不同生物学性状的形成机制;挖掘与生命活动和疾病相关的关键基因和信号通路;推动农业科学、生命科学和医学等领域的快速发展。文中就三维基因组学研究进展作一综述,主要阐述三维基因组学的概念和研究技术的发展及其在农业科学、生命科学和医学等领域的应用,尤其是肿瘤领域所取得的阶段性研究成果。

Massive individual orbital angular momentum channels for multiplexing enabled by Dammann gratings

Data transmission rates in optical communication systems are approaching the limits of conventional multiplexing methods.Orbital angular momentum(OAM)in optical vortex beams offers a new degree of freedom and the potential to increase the capacity of free-space optical communication systems,with OAM beams acting as information carriers for OAM division multiplexing(OAM-DM).We demonstrate independent collinear OAM channel generation,transmission and simultaneous detection using Dammann optical vortex gratings(DOVGs).We achieve 80/160 Tbit s^(-1) capacity with uniform power distributions along all channels,with 1600 individually modulated quadrature phase-shift keying(QPSK)/16-QAM data channels multiplexed by 10 OAM states,80 wavelengths and two polarizations.DOVG-enabled OAM multiplexing technology removes the bottleneck of massive OAM state parallel detection and offers an opportunity to raise optical communication systems capacity to Pbit s^(-1) level.

Joint timing and frequency synchronization in coherent optical OFDM systems

In this paper,we review our joint timing and frequency synchronization algorithms in coherent optical orthogonal frequency division multiplexing(CO-OFDM)systems.We first present a timing estimation method by designing the pattern of the training symbol,whose timing metric has a sharp and clear peak,to ensure accurate timing offset(TO)estimation performance.Then we provide both data-aided(DA)and blind(BL)approaches to estimate the carrier frequency offset(CFO).For the DA algorithm,we utilize the same training symbol structure as the timing estimation does,while for the BL algorithm,we utilize the zero-subcarrier power(ZSP)to achieve full-range CFO estimation.Note that our joint timing and frequency synchronization approaches require only one OFDM symbol,which ensure not only the data transmission efficiency,but also the TO and CFO estimation performance.A modified BL ZSP algorithm is proposed to further improve the CFO estimation performance by taking the power average over a series of OFDM symbols.The effectiveness of the TO estimation algorithm,and both the DA and BL CFO estimation algorithms are verified and demonstrated in both simulations and experiments.

Plasmonic bound states in the continuum for unpolarized weak spatially coherent light

Plasmonic resonances empowered by bound states in the continuum(BICs) offer unprecedented opportunities to tailor light–matter interaction. However, excitation of high quality-factor(Q-factor) quasi-BICs is often limited to collimated light at specific polarization and incident directions, rendering challenges for unpolarized focused light. The major hurdle is the lack of robustness against weak spatial coherence and poor polarization of incident light. Here, addressing this limitation, we demonstrate sharp resonances in symmetric plasmonic metasurfaces by exploiting BICs in the parameter space, offering ultraweak angular dispersion effect and polarization-independent performance. Specifically, a high-Q(≈71) resonance with near-perfect absorption(>90%) is obtained for the input of unpolarized focused light covering wide incident angles(from 0° to 30°). Also, giant electric and magnetic field enhancement simultaneously occurs in quasi-BICs. These results provide a way to achieve efficient near-field enhancement using focused light produced by high numerical aperture objectives.

Phyllotaxis-inspired nanosieves with multiplexed orbital angular momentum

Nanophotonic platforms such as metasurfaces,achieving arbitrary phase profiles within ultrathin thickness,emerge as miniaturized,ultracompact and kaleidoscopic optical vortex generators.However,it is often required to segment or interleave independent sub-array metasurfaces to multiplex optical vortices in a single nano-device,which in turn affects the device’s compactness and channel capacity.Here,inspired by phyllotaxis patterns in pine cones and sunflowers,we theoretically prove and experimentally report that multiple optical vortices can be produced in a single compact phyllotaxis nanosieve,both in free space and on a chip,where one meta-atom may contribute to many vortices simultaneously.The time-resolved dynamics of on-chip interference wavefronts between multiple plasmonic vortices was revealed by ultrafast time-resolved photoemission electron microscopy.Our nature-inspired optical vortex generator would facilitate various vortex-related optical applications,including structured wavefront shaping,free-space and plasmonic vortices,and high-capacity information metaphotonics.

Broadband instantaneous multi-frequency measurement based on chirped pulse compression

A broadband instantaneous multi-frequency measurement system based on chirped pulse compression,which potentially has a sub-megahertz(MHz)accuracy and a hundred-gigahertz(GHz)measurement range,is demonstrated.A signalunder-test(SUT)is converted into a carrier-suppressed double-sideband(CS-DSB)signal,which is then combined with an optical linearly frequency-modulated signal having the sweeping range covering the+1 st-order sideband of the CSDSB signal.With photodetection,low-pass filtering,and pulse compression,accurate frequencies of the SUT are obtained via locating the correlation peaks.In the experiment,single-and multi-frequency measurements with a measurement range from 3 to 18 GHz and a measurement accuracy of<±100 MHz are achieved.

Carrier recovery in coherent receiver of optical orthogonal frequency division multiplexing system

In this paper, we reviewed our common phase error （CPE） and intercarrier interference （ICI） compensation methods for coherent optical orthogonal frequency division multiplexing （CO-OFDM） system. We first presented a unified CPE estimation framework combining decision-aided （DA）, pilot-aided （PA） and decision feed- back （DF） algorithms. The DA method is used to estimate the CPE of the current OFDM symbol based on the decision statistics of the previous symbol. DA ＋ PA helps increase the phase noise tolerance of DA and reduce the overhead of PA, while DA ＋ DF reduces the overhead to zero, achieving best performance with one more step of estimation, compensation and demodulation. We also described a modified time-domain blind intercarrier interference （BL-ICI） mitigation algorithm over non- constant amplitude formats. The new algorithm is derived from the BL-ICI algorithm over constant amplitude format for wireless networks. A new power estimation scheme was proposed for the BL-ICI algorithm to adapt to non- constant amplitude format. It has the same order of complexity with frequency domain decision-aided ICI （DA-ICI） compensation method and does not suffer from symbol decision errors. The effectiveness of both CPE and ICI compensation algorithms were demonstrated in a simulated 56-Gbit/s CO-OFDM system with various modulation formats.

Real-time full-color meta fiber endoscopy

The remarkable capacity of metasurfaces to exert precise control over the propagation of light has ushered in a realm of unprecedented possibilities for"Lab-on-fiber",and in this work,showcased real-time full-color imaging in a meta-optica l fiber endoscope.

Polymorphism analysis and supertype definition of swine leukocyte antigen class I molecules in three-way crossbred(Landrace,Duroc,and Yorkshire)pigs:implications for the vaccine development of African swine fever virus

Dear Editor,Swine major histocompatibility complex(MHC)is a highly polymorphic gene in pigs and is also called swine leukocyte antigen(SLA)(Fan et al.,2018).SLA is divided into three major categories,SLA I(SLA-1,-2,-3),SLA II,and SLA III(Smith et al.,2005).SLA I plays an important role in cellular immunity which can eliminate viruses and other foreign antigens in pigs(Macdonald et al.,2010).SLA-1,SLA-2 and SLA-3 are important SLA I alleles and are highly polymorphic.Polymorphism causes extreme difficulties in screening broadly protective cytotoxic lymphocyte(CTL)epitope antigens.The supertype which is built based on peptide-binding specificity of each SLA I can overcome this difficulty to some extent(Thomsen et al.,2013).

Performance comparison among three different Stokes vector direct-detection receivers

Stokes vectors direct detection(SV-DD)is an effective solution for short-reach optical communications.In this Letter,we investigate two-dimensional-modulation direct-detection systems based on three Stokes vector receivers(SVRs).The influences of three key factors including the states-of-polarization(SOP),the splitting ratio of the coupler,and the excess loss(EL)are studied in detail.It is shown that the splitting ratio for achieving optimum performance will be changed with SOP and EL conditions.Among these SVRs,the3×3 coupler-based receiver with its optimal splitting ratio shows the best bit error rate performance and stability against the change of SOP.