Antioxidant and Hypoglycemic Ability of Ardisia gigantifolia Stapf Parts

[Objectives]To study the antioxidant and hypoglycemic effects of different parts of Ardisia gigantifolia Stapf.[Methods]The hydroxyl radical scavenging activity,DPPH radical scavenging activity and total antioxidant capacity of ABTS of 75%ethanol extract of A.gigantifolia Stapf and the petroleum ether,ethyl acetate,n-butanol,chloroform and aqueous extract were measured with Vc as positive control.At the same time,acarbose was used as reference substance to determine the inhibitory effect of each polar site onα-glucosidase.[Results]All parts of A.gigantifolia Stapf had antioxidant activity,among which ethyl acetate had the strongest antioxidant activity,and the scavenging rate of hydroxyl radical and DPPH radical was higher than that of positive control.The results showed that petroleum ether,ethyl acetate and chloroform had a good inhibitory effect onα-glucosidase(better than acarbose).[Conclusions]The ethyl acetate part of A.gigantifolia Stapf had the best antioxidant activity and inhibitory effect onα-glucosidase.It provides a basis for further research and development of A.gigantifolia Stapf.

Optimization of Solid-state Fermentation Conditions of Sophora japonica cv.jinhuai by Response Surface Methodology

[Objectives]To optimize the solid-state fermentation process of Flos Sophorae Immaturus by Penicillium with Sophora japonica cv.jinhuai as raw material.[Methods]The fermentation conditions were optimized by single factor experiment and response surface methodology with quercetin content as the dependent variable.[Results]According to the established model,the optimal fermentation process of Flos Sophorae Immaturus was obtained as follows:temperature 29.97℃,time 6.88 d,rotation speed 180.86 rpm,inoculation amount 3.93 mL,and the expected content of quercetin was 34.8053 mg/g.Based on this,the fermentation parameters were adjusted,and the actual content was 33.67 mg/g,which was close to the predicted value.[Conclusions]The optimization of fermentation process of Flos Sophorae Immaturus by response surface methodology provides a reference for the development and utilization of this medicinal material.

Effects of Polysaccharide from Polygala fallax on Apoptosis of B16 Melanoma

[Objectives]The paper was to study the effects of polysaccharide from Polygala fallax on apoptosis of B16 melanoma and its mechanism.[Methods]P.fallax polysaccharide was prepared by water extraction and alcohol precipitation method combined with Sevag method.The effects of different concentrations of P.fallax polysaccharide on B16 cell proliferation were detected by MTT assay.Apoptosis was detected by Hoechst 33258 staining assay and flow cytometry.The expressions of Bcl-2,Bax,Cleaved Caspase-3 mRNA were detected by Real-time PCR assay.The expressions of Bcl-2,Bax,Caspase-3,Caspase-8,Caspase-9,Cleaved Caspase-3,Cleaved Caspase-9,ERK,p-ERK,JNK,p-JNK proteins were detected by Western Blot assay.[Results]P.fallax polysaccharide inhibited the cell proliferation of B16 melanoma in a concentration-dependent manner(24 h IC 50=0.2133 mg/L;48 h IC 50=0.08489μg/mL).P.fallax polysaccharide up-regulated the expressions of Bax,Cleaved Caspase-3 mRNA and protein,down-regulated the expressions of Bcl-2 mRNA and protein,activated Caspase-3,Caspase-8 and Caspase-9,and decreased the levels of p-ERK/ERK and p-JNK/JNK.[Conclusions]P.fallax polysaccharide can inhibit the cell proliferation of B16 melanoma and induce their apoptosis,probably attributed to the regulation of MAPK,Bcl-2 and Caspase family signaling pathways.