Chenghua(CH)pig and Qingyu(QY)pig are typical Chinese native fatty breeds.CH pig is mainly distributed in Chengdu Plain,while QY pig is widely distributed throughout the mountain areas around the Sichuan Basin.There a...Chenghua(CH)pig and Qingyu(QY)pig are typical Chinese native fatty breeds.CH pig is mainly distributed in Chengdu Plain,while QY pig is widely distributed throughout the mountain areas around the Sichuan Basin.There are significant differences in their phenotypic traits,including body image,growth performance,and meat quality.This study compared several meat quality traits of CH and QY pigs and conducted a genome-wide DNA methylation analysis using reduced representation bisulfite sequencing(RRBS).It was observed that the pH at 45 min(pH_(45min),P=5.22e-13),lightness at 45 min(L^(*)_(45min),P=4.85e-5),and lightness at 24 h(L^(*)_(24h),P=3.57e-5)of CH pigs were higher than those of QY pigs.We detected 10699 differentially methylated cytosines(DMCs)and 2760 differentially methylated genes(DMGs)associated with these DMCs.Functional analysis showed that these DMGs were mainly enriched in the AMPK signaling pathway,Type II diabetes mellitus,Insulin signaling pathway,mTOR signaling pathway,and Insulin resistance.Furthermore,15 DMGs were associated with fat metabolism(ACACA,CAB39,CRADD,CRTC2,FASN,and GCK),muscle development(HK2,IKBKB,MTOR,PIK3CD,PPARGC1A,and RPTOR),or meat quality traits(PCK1,PRKAG2,and SLC2A4).The findings may help to understand further the epigenetic regulation mechanisms of meat quality traits in pigs and provide new basic data for the study of local pigs.展开更多
文章探讨了锻炼态度对体育学习投入的影响以及自我效能感的中介作用.采用体育锻炼态度量表、学习投入量表和自我效能量表,选取甘肃省4所高职院校784名大学生进行问卷调查.结果表明,锻炼态度对体育学习投入呈显著正相关(β=0.994, p <...文章探讨了锻炼态度对体育学习投入的影响以及自我效能感的中介作用.采用体育锻炼态度量表、学习投入量表和自我效能量表,选取甘肃省4所高职院校784名大学生进行问卷调查.结果表明,锻炼态度对体育学习投入呈显著正相关(β=0.994, p <0.001);自我效能感对体育学习投入呈显著正相关(β=1.078, p <0.001);自我效能感在两者之间具有完全中介作用,且自我效能感的效应占比达84%.由此得出结论:锻炼态度、自我效能感和体育学习投入三者间具有明显正相关性;通过提高锻炼态度可以增强体育学习投入的积极性,也可以通过提高自我效能感间接提高体育学习投入.展开更多
基金supported by the grants from the Sichuan Science and Technology Program, China (2020YFN0024)the China Agriculture Research System of MOF and MARA (CARS-35-01A)+3 种基金the National Key R&D Program of China (2018YFD0501204)the National Natural Science Foundation of China (C170102)the Sichuan Innovation Team of Pig, China (sccxtd-2021-08)supported by the High-performance Computing Platform of Sichuan Agricultural University, China
文摘Chenghua(CH)pig and Qingyu(QY)pig are typical Chinese native fatty breeds.CH pig is mainly distributed in Chengdu Plain,while QY pig is widely distributed throughout the mountain areas around the Sichuan Basin.There are significant differences in their phenotypic traits,including body image,growth performance,and meat quality.This study compared several meat quality traits of CH and QY pigs and conducted a genome-wide DNA methylation analysis using reduced representation bisulfite sequencing(RRBS).It was observed that the pH at 45 min(pH_(45min),P=5.22e-13),lightness at 45 min(L^(*)_(45min),P=4.85e-5),and lightness at 24 h(L^(*)_(24h),P=3.57e-5)of CH pigs were higher than those of QY pigs.We detected 10699 differentially methylated cytosines(DMCs)and 2760 differentially methylated genes(DMGs)associated with these DMCs.Functional analysis showed that these DMGs were mainly enriched in the AMPK signaling pathway,Type II diabetes mellitus,Insulin signaling pathway,mTOR signaling pathway,and Insulin resistance.Furthermore,15 DMGs were associated with fat metabolism(ACACA,CAB39,CRADD,CRTC2,FASN,and GCK),muscle development(HK2,IKBKB,MTOR,PIK3CD,PPARGC1A,and RPTOR),or meat quality traits(PCK1,PRKAG2,and SLC2A4).The findings may help to understand further the epigenetic regulation mechanisms of meat quality traits in pigs and provide new basic data for the study of local pigs.
文摘文章探讨了锻炼态度对体育学习投入的影响以及自我效能感的中介作用.采用体育锻炼态度量表、学习投入量表和自我效能量表,选取甘肃省4所高职院校784名大学生进行问卷调查.结果表明,锻炼态度对体育学习投入呈显著正相关(β=0.994, p <0.001);自我效能感对体育学习投入呈显著正相关(β=1.078, p <0.001);自我效能感在两者之间具有完全中介作用,且自我效能感的效应占比达84%.由此得出结论:锻炼态度、自我效能感和体育学习投入三者间具有明显正相关性;通过提高锻炼态度可以增强体育学习投入的积极性,也可以通过提高自我效能感间接提高体育学习投入.
