Mitochondria undergo morphological changes during spermatogenesis in some animals.The mechanism and role of mitochondrial morphology regulation,however,remain somewhat unclear.In this study,we analyzed the molecular c...Mitochondria undergo morphological changes during spermatogenesis in some animals.The mechanism and role of mitochondrial morphology regulation,however,remain somewhat unclear.In this study,we analyzed the molecular characteristics,expression dynamics and subcellular localization of optic atrophy protein 1(OPA1),a mitochondrial fusion and cristae maintenance-related protein,to reveal the possible regulatory mechanisms underlying mitochondrial morphology in Phascolosoma esculenta spermiogenesis.The full-length cDNA of the P.esculenta opa1 gene(Pe-opa1)is 3743 bp in length and encodes 975 amino acids.The Pe-OPA1 protein is highly conservative and includes a transmembrane domain,a GTPase domain,two helical bundle domains,and a lipid-interacting stalk.Gene and protein expression was higher in the coelomic fluid(a site of spermatid development)of male P.esculenta and increased first and then decreased from March to December.Moreover,their expression during the breeding stage was significantly higher than during the non-breeding stage,suggesting that Pe-OPA1 is involved in P.esculenta reproduction.The Pe-OPA1 protein was more abundant in components consisting of many spermatids than in components without,indicating that Pe-OPA1 mainly plays a role in the spermatid in coelomic fluid.Moreover,Pe-OPA1 was mainly detected in the spermatid mitochondria.Immunofluorescence experiments showed that the Pe-OPA1 are constitutively expressed and co-localized with mitochondria during spermiogenesis,suggesting its involvement in P.esculenta spermiogenesis.These results provide evidence for Pe-OPA1's involvement in the regulation of mitochondrial morphology during spermiogenesis.展开更多
针对当前拖拉机检测系统功能集成度低、检测参数不全面、传输距离有限的问题,开发了拖拉机田间作业参数无线检测系统。该系统由传感器、数据采集仪及上位机软件监测平台3部分组成,能够实现PTO转矩及转速、油耗、发动机转速、悬挂提升力...针对当前拖拉机检测系统功能集成度低、检测参数不全面、传输距离有限的问题,开发了拖拉机田间作业参数无线检测系统。该系统由传感器、数据采集仪及上位机软件监测平台3部分组成,能够实现PTO转矩及转速、油耗、发动机转速、悬挂提升力、力位调节加载力、加载角度、行驶速度、车轮转速、牵引力等多种参数的采集、无线发送与存储。系统工作时,数据采集仪中的车载检测仪将采集的传感器数据发送至无线数据接收器,无线数据接收器通过串口将数据传输至上位机软件监测平台,实现对各类试验参数的实时监测与数据处理。为验证检测系统的可行性与稳定性,对系统进行了采集通道的计量,结果显示模拟信号通道绝对误差绝对值最大为0.003 V,引用误差最大为0.03%,频率信号通道检测绝对误差最大为2 Hz,引用误差最大为0.013%,满足对拖拉机作业参数的采集需求。在此基础上,进行了PTO转矩参数及拖拉机无负载行驶速度采集试验。试验结果表明,检测系统可以实现转矩参数的稳定采集及数据的无线传输;在5、8、14 km/h 3挡车速匀速行驶下,拖拉机车轮转速与实际行驶速度基本一致,最大相对误差分别为2.0%、1.2%及0.7%。本系统可满足对拖拉机工作性能参数的无线检测需求,数据采集稳定且采集精度较高,为拖拉机多作业参数的无线采集提供有效手段。展开更多
基金the Ningbo Science and Technology Plan Projects(Nos.2019B10016,2016C10004)the Major Science and Technology Projects in Zhejiang Province(No.2011C12013)+1 种基金the Natural Science Foundation of Zhejiang Province(No.LY18C190007)the Collaborative Innovation Center for Zhejiang Marine High-efficiency and Healthy Aquaculture,the K.C.Wong Magna Fund in Ningbo University。
文摘Mitochondria undergo morphological changes during spermatogenesis in some animals.The mechanism and role of mitochondrial morphology regulation,however,remain somewhat unclear.In this study,we analyzed the molecular characteristics,expression dynamics and subcellular localization of optic atrophy protein 1(OPA1),a mitochondrial fusion and cristae maintenance-related protein,to reveal the possible regulatory mechanisms underlying mitochondrial morphology in Phascolosoma esculenta spermiogenesis.The full-length cDNA of the P.esculenta opa1 gene(Pe-opa1)is 3743 bp in length and encodes 975 amino acids.The Pe-OPA1 protein is highly conservative and includes a transmembrane domain,a GTPase domain,two helical bundle domains,and a lipid-interacting stalk.Gene and protein expression was higher in the coelomic fluid(a site of spermatid development)of male P.esculenta and increased first and then decreased from March to December.Moreover,their expression during the breeding stage was significantly higher than during the non-breeding stage,suggesting that Pe-OPA1 is involved in P.esculenta reproduction.The Pe-OPA1 protein was more abundant in components consisting of many spermatids than in components without,indicating that Pe-OPA1 mainly plays a role in the spermatid in coelomic fluid.Moreover,Pe-OPA1 was mainly detected in the spermatid mitochondria.Immunofluorescence experiments showed that the Pe-OPA1 are constitutively expressed and co-localized with mitochondria during spermiogenesis,suggesting its involvement in P.esculenta spermiogenesis.These results provide evidence for Pe-OPA1's involvement in the regulation of mitochondrial morphology during spermiogenesis.
文摘针对当前拖拉机检测系统功能集成度低、检测参数不全面、传输距离有限的问题,开发了拖拉机田间作业参数无线检测系统。该系统由传感器、数据采集仪及上位机软件监测平台3部分组成,能够实现PTO转矩及转速、油耗、发动机转速、悬挂提升力、力位调节加载力、加载角度、行驶速度、车轮转速、牵引力等多种参数的采集、无线发送与存储。系统工作时,数据采集仪中的车载检测仪将采集的传感器数据发送至无线数据接收器,无线数据接收器通过串口将数据传输至上位机软件监测平台,实现对各类试验参数的实时监测与数据处理。为验证检测系统的可行性与稳定性,对系统进行了采集通道的计量,结果显示模拟信号通道绝对误差绝对值最大为0.003 V,引用误差最大为0.03%,频率信号通道检测绝对误差最大为2 Hz,引用误差最大为0.013%,满足对拖拉机作业参数的采集需求。在此基础上,进行了PTO转矩参数及拖拉机无负载行驶速度采集试验。试验结果表明,检测系统可以实现转矩参数的稳定采集及数据的无线传输;在5、8、14 km/h 3挡车速匀速行驶下,拖拉机车轮转速与实际行驶速度基本一致,最大相对误差分别为2.0%、1.2%及0.7%。本系统可满足对拖拉机工作性能参数的无线检测需求,数据采集稳定且采集精度较高,为拖拉机多作业参数的无线采集提供有效手段。
