AIM To explore the expression of perform andgranzyme B genes mRNA to judge the effect ofimmunosuppression in acute rejection of livertransplantation.METHODS The expression of perform andgranzyme B genes mRNA was exami...AIM To explore the expression of perform andgranzyme B genes mRNA to judge the effect ofimmunosuppression in acute rejection of livertransplantation.METHODS The expression of perform andgranzyme B genes mRNA was examined byreverse transcriptase-polymerase chain reaction(RT--PCR) in hamster to rat liver grafts under theimmunosuppression of cyclosporine or/andsplenectomy. Histological findings were studiedcomparatively.RESULTS Cyclosporine could obviouslydecrease the cellular infiltration, and completelyrepress the expression of mRNA for perform andgranzyme B, but could not change severehepatocyte necrosis and hemorrhage.SPlenectomy could significantly lightenhepatocyte necrosis, and completely eliminatehemorrhage, but not atfect the cellularinfiltration and the expression of perform andgranzyme B genes mRNA. Cyclosporine orsplenectomy alone could not prolong theSurvival time, however, their combination couldcompletely repress the rejection of liver grafts.The survival time of animals were significantlyprolonged (37.1 days ). The architecture ofhepatic lobules was preserved. There was slightcellular infiltration in the portal tracts and noexpression of perform and granzyme B genesmRNA could be seen in three weeks aftertransplantation.CONCLUSION Perform and granzyme B genesare valuable in judging the effect ofimmunosuppression in liver transplantation.展开更多
该研究纳入参加随机对照试验——＂控制糖尿病患者心血管风险行动＂试验中的4331例糖尿病患者,比较强化治疗（收缩压〈120 mm Hg,1 mm Hg=0.133kPa）与标准治疗（收缩压〈140 mm Hg）的作用,探讨降压治疗对左心室肥厚（left ventricular...该研究纳入参加随机对照试验——＂控制糖尿病患者心血管风险行动＂试验中的4331例糖尿病患者,比较强化治疗（收缩压〈120 mm Hg,1 mm Hg=0.133kPa）与标准治疗（收缩压〈140 mm Hg）的作用,探讨降压治疗对左心室肥厚（left ventricular hypertrophy,LVH）风险的影响。采用Cornell电压（二分类变量）及平均Cornell指数（连续变量）评估心电图LVH。基线时强化治疗组（n=2154）与标准治疗组（n=2177）的LVH发生率（5.3%比5.4%,展开更多
基金Project supported by the National Natural Science Foundation of China,No.39500152.
文摘AIM To explore the expression of perform andgranzyme B genes mRNA to judge the effect ofimmunosuppression in acute rejection of livertransplantation.METHODS The expression of perform andgranzyme B genes mRNA was examined byreverse transcriptase-polymerase chain reaction(RT--PCR) in hamster to rat liver grafts under theimmunosuppression of cyclosporine or/andsplenectomy. Histological findings were studiedcomparatively.RESULTS Cyclosporine could obviouslydecrease the cellular infiltration, and completelyrepress the expression of mRNA for perform andgranzyme B, but could not change severehepatocyte necrosis and hemorrhage.SPlenectomy could significantly lightenhepatocyte necrosis, and completely eliminatehemorrhage, but not atfect the cellularinfiltration and the expression of perform andgranzyme B genes mRNA. Cyclosporine orsplenectomy alone could not prolong theSurvival time, however, their combination couldcompletely repress the rejection of liver grafts.The survival time of animals were significantlyprolonged (37.1 days ). The architecture ofhepatic lobules was preserved. There was slightcellular infiltration in the portal tracts and noexpression of perform and granzyme B genesmRNA could be seen in three weeks aftertransplantation.CONCLUSION Perform and granzyme B genesare valuable in judging the effect ofimmunosuppression in liver transplantation.
文摘该研究纳入参加随机对照试验——＂控制糖尿病患者心血管风险行动＂试验中的4331例糖尿病患者,比较强化治疗（收缩压〈120 mm Hg,1 mm Hg=0.133kPa）与标准治疗（收缩压〈140 mm Hg）的作用,探讨降压治疗对左心室肥厚（left ventricular hypertrophy,LVH）风险的影响。采用Cornell电压（二分类变量）及平均Cornell指数（连续变量）评估心电图LVH。基线时强化治疗组（n=2154）与标准治疗组（n=2177）的LVH发生率（5.3%比5.4%,
