p-Hydrazinobenzenesulfonic Acid Derivatives of Carbohydrates andTheir Capillary Zone Electrophoresis

p-Hydrazinobenzenesulfonic acid is explored as a novel ultraviolet labeling reagent for capillary electrophoresis (CE) of mono- and disaccharides. The labeling reaction takes less than 10 minutes and introduces both of absorption and charge groups into the sugars.

Research on Characteristics of Density Current Under the Action of Waves

In this paper, the characteristics of density current under the action of waves are described with the help of flume experiment and theoretical analysis. The study shows that turbid water under the action of the waves can present three types of motion, i. e. significant stratification, fragile stratification and strong mixing. The motion gf turbid;,ater presents significant stratification when (H/D)/root Delta rho/rho less than or equal to 4.5, generally this state is known as density current. The formulas of motion velocity, thickness, and discharge of density current moving on horizontal bottom are derived by use of basic equations such as momemtum equation, equation of energy conservation and continuity equation of fluid. The time-average velocity and the thickness of density current under the action of waves have a relationship with such parameters as relative density (Delta rho/rho), wave height (H), and water depth (D). When these parameters are determined, the time-average thickness and motion velocity of density current are also determined. The relative thickness of density current (D-t/D) decreases with the increase of Delta rho/rho and increases with the increase of H/D. On the other hand, the motion velocity of density current increases with the increase of Delta rho/rho and decreases with the increase of the relative thickness (D-t/D) of density current. It is shown that the calculated results are in agreement with those of the flume experiment.

Capillary Electrophoretic Separation of Monosialogangliosides by using cyclodextrin and borax as selectors

f The CE separation of monosialogangliosids (MSGLS) is generally difficult owing to theirmixed micelle formation. The addition of high concentration of borax to the buffer containing α-Cyclodextrin allowed for improvement of selectivity of separation. Under the optimum conditions,several forms of MSGLS were successfully separated and the commercial standard GM1 gave twopeaks, suggesting that the standard may contain two different types of species.

STUDIES OF THE INTERACTION BETWEEN METALLOPORPHYRINS OF TMPYP－3 AND SINGLE－STRANDED DNA ON NC MEMBRANE

We report here a fast staining method for single-stranded DNA fixed on nitrocellulose membrane using M-meso-letra(3-N-methylpyridyl)-porphrin (M-TMPyP, M: Mn3, Fe3, Pt3). The optimal staining solution consists of 54-108 μg/ml M-TMPyP in 25～200 mmol/l. phosphate buffer solution (pH 5.0～8.0).When DNA dot on NC membrane is treated with the staining solution at room temperature for 1～2 minutes with continuous stirring, it turns to brown-yellow.10pg per dot of single-stranded DNA is visible. It is a useful tool to sludy the DNA blotting, and for recognition of the special bands of DNA in a complete electrophoretogram of DNA blot. The staining mechanism is also discussed.The staining techniques of DNA play a important role in DNA blotting,display of DNA elcctophoretogram and location of the special DNA band from it.Since R.J. Fiel and co-workers[1] reported that meso-tetra(4-N-methylpyridyl) porphyrin can intercalale in calf thymus DNA. the intercalation between TMPyP-4 and DNA in solution has been intensively investigated by several groups[2～8],but none of them concerned with the interaction of metalloporphyrin and filterbound DNA. In this report, the use of M-TMPyP as a fast and sensitive staining reagent for single-strand DNA on NC membrane is described.

Fractal Growth and Form by Molecular Dynamics

The fractal growth and form is considered by molecular dynamics and simulated using computer.

HIGH－PERFORMANCE CAPILLARY ELECTROPHORESIS OF REDUCING OLIGOSACCHARIDES AS THEIR ETHYL P－AMINOBENZOATE DERIVATIVES

A modified method for the separation of oligosaccharides derivatized with p-aminoben-zoate ethyl ester by high performance capillary electrophoresis (HPCE) is described. The isomers of larger oligosaccharides up to 10-mers were separated by HPCE with borax as running buffer.

CAPILLARY ELECTROPHORESIS DETERMINATION OF INOSINE IN SOMEDRUG FORMULAE USING HYPOXANTHINE AS INTERNAL STANDARD

A new rapid and accurate method for determining inosine by capillary zone electrophoresis(CZE) is presented. The electrophoresis separation was performed at 24℃ in a fused-silica capillary(51.2cm×50 μmi.d., 38.5cm to UV detector)using 20mmol/L borax-20mmol/L H3PO4(pH 8.5) as the background electrolyte at 24KV applied voltage. Inosine samples were led into the capillary electrokinetically at 10KV for 15 seconds. In the range of 20μg/mL to 300μg/mL, inosine can be quantitatively determined with a deduction limit of 2.1μg/mL and a RSD of 2.92%. Hypoxanthine was used as the internal standard.

Numerical Simulation of Wave Field at the Kemena River Mouth in Kalimantan Island

By use of the parabolic equation of numerical simulation of wave which is suitable for large-angle propagation anti Crank-Nicolson differential method, the wave field at the Kemema River mouth has been studied for analysis of sediment movement in the area. In order to reflect wave energy loss accurately, the Bretchneider-Reid formula is quoted and the friction coefficient in the formula is discussed in this paper. The calculation results indicate that the wave becomes a little damped at the mouth of Kemena River influenced by the topography and bottom friction, and the wave at the east beach is higher than that at the west beach, because the east beach extends out.

A New Annonaceous Acetogenin from Goniothalamus Gardneri

A new monotetrahydrofuran Annonaceous acetogenin, gardnerin (1) was isolated from the roots of Goniothalamus gardneri Hook. f. et. Thoms. This compound possesses five hydroxyl groups, with two hydroxyl groups at C-8 and C-10 positions. The absolute stereochemistry at 8-OH and 10-OH positions Nas determined by converting 1 to its ketolactone isomer, 2,4-cis/trans gardnerin. Bioactivities of this new compound against human tumor cell lines are reported. Five known acetogenin compounds gigantetrocin A and B, annonacin, goniothalamicin, isoannonacin were also found.

Absolute Configurations of Tonkinin A and B: Two New Acetogenins from Uvaria Tonkinensis

The absolute stereochemistries of Annonaceous acetogenins, tonkinin A and B, have been established by the use of the convenient conversion to their formaldehyde acetal derivatives and the subsequent application of the Mosher ester methodology.

A new type of oceanic crust and its dynamical significance

THE oceanic lithosphere is composed of oceanic crust and upper mantle. It is widely accepted that the Troodos ophiolitic section represents the oceanic crust section, which consists of ultramafic cumulates in the bottom, and grades upward into cumulative gabbros, isotropic gabbro, sheeted dikes and pillow lavas, covered by abyssal sediments. According to the marine geophysical investigation, the oceanic crust can be divided into three layers which from top to bot-

Inheritance of callus induction ability in rice anther culture

DURING the last two decades, anther culture technique has been widely used in plant improve-ment including rice, wheat and maize. Recently, doubled haploid (DH) progenies derivedfrom anther culture have also been used in molecular mapping projects as permanent popula-tions. Some studies have revealed that the rice culturability is controlled by a few genetic fac-tors. Xue suggested that it was an inheritable trait with additive gene effect. Wu

SUPERCONVERGENCE OF DISCONTINUOUS GALERKIN METHOD FOR NONSTATIONARY HYPERBOLIC EQUATION

For the first order nonstationary hyperbolic equation taking the piecewise linear discontinuous Galerkin solver, we prove that under the uniform rectangular partition, such a discontinuous solver, after postprossesing, can have two and half approximative order which is half order higher than the optimal estimate by Lesaint and Raviart under the rectangular partition.

A chemical index of weather-ing without effect of wind sorting: Fe/Mg ratios in the acid-insoluble phases of loess deposits

Chemical analysis of acid-insoluble fractions in loess and paleosols shows that concentrations of Fe and Mg were under control of wind sorting and post-depositional weathering-pedogenesis. The former caused Fe and Mg concentrated in the finer grain-size fractions, displaying synchronous variations, while the latter made Fe and Mg separated, leading to Fe retained in the weathered section and Mg leached out. Therefore, Fe/Mg ratios in the acid insoluble fraction of loess and paleosols can eliminate the effect of wind sorting and serve as an excellent proxy record on intensity of weathering-pedogenesis. Based on calculation, leaching percentage of Mg in the paleosol S1 from the Luochuan, Xifeng and Huanxian sections is 15%, 11% and 2%, respectively, and on average 9% for the paleosols S2—S14 from the Luochuan section, with the highest value amounting to 22% in S5-1, suggesting the strongest weathering-pedogenesis.

Mechanism of voltage tunable electroluminescence of porous silicon

ALTHOUGH electroluminescence(EL)from silicon anodes in contact with various electrolyteswas first reported by Gee in 1960,it did not attract much attention until Canham in 1990obtained highly efficient visible photoluminescence(PL)on porous silicon(PS)at room tem-perature.This is important for both fundamental interest in nanocrystalline