The P-type plasma membrane(PM)H^(+)-ATPases(HAs)are crucial for plant development,growth,and defense.The HAs have been thoroughly characterized in many different plants.However,despite their importance,the functions o...The P-type plasma membrane(PM)H^(+)-ATPases(HAs)are crucial for plant development,growth,and defense.The HAs have been thoroughly characterized in many different plants.However,despite their importance,the functions of HAs in germination and seed dormancy(SD)have not been validated in wheat.Here,we identified 28 TaHA genes(TaHA1-28)in common wheat,which were divided into five subfamilies.An examination of gene expression in strong-and weak-SD wheat varieties led to the discovery of six candidate genes(TaHA7/-12/-14/-16/-18/-20).Based on a single nucleotide polymorphism(SNP)mutation(C/T)in the TaHA7 coding region,a CAPS marker(HA7)was developed and validated in 168 wheat varieties and 171 Chinese mini-core collections that exhibit diverse germination and SD phenotypes.We further verified the roles of the two allelic variations of TaHA7 in germination and SD using wheat mutants mutagenized with ethyl methane sulphonate(EMS)in‘Jimai 22’and‘Jing 411’backgrounds,and in transgenic Arabidopsis lines.TaHA7 appears to regulate germination and SD by mediating gibberellic acid(GA)and abscisic acid(ABA)signaling,metabolism,and biosynthesis.The results presented here will enable future research regarding the TaHAs in wheat.展开更多
The heat transfer and stability of methane hydrate in reservoirs have a direct impact on the drilling and production efficiency of hydrate resources,especially in complex stress environments caused by formation subsid...The heat transfer and stability of methane hydrate in reservoirs have a direct impact on the drilling and production efficiency of hydrate resources,especially in complex stress environments caused by formation subsidence.In this study,we investigated the thermal transport and structural stability of methane hydrate under triaxial compression using molecular dynamics simulations.The results suggest that the thermal conductivity of methane hydrate increases with increasing compression strain.Two phonon transport mechanisms were identified as factors enhancing thermal conductivity.At low compressive strains,a low-frequency phonon transport channel was established due to the overlap of phonon vibration peaks between methane and water molecules.At high compressive strains,the filling of larger phonon bandgaps facilitated the opening of more phonon transport channels.Additionally,we found that a strain of0.04 is a watershed point,where methane hydrate transitions from stable to unstable.Furthermore,a strain of0.06 marks the threshold at which the diffusion capacities of methane and water molecules are at their peaks.At a higher strain of0.08,the increased volume compression reduces the available space,limiting the diffusion ability of water and methane molecules within the hydrate.The synergistic effect of the strong diffusion ability and high probability of collision between atoms increases the thermal conductivity of hydrates during the unstable period compared to the stable period.Our findings offer valuable theoretical insights into the thermal conductivity and stability of methane hydrates in reservoir stress environments.展开更多
Horizontal well drilling and multi-stage hydraulic fracturing technologies are at the root of commercial shale gas development and exploitation.During these processes,typically,a large amount of working fluid enters t...Horizontal well drilling and multi-stage hydraulic fracturing technologies are at the root of commercial shale gas development and exploitation.During these processes,typically,a large amount of working fluid enters the formation,resulting in widespread water-rock interaction.Deeply understanding such effects is required to optimize the production system.In this study,the mechanisms of water-rock interaction and the associated responses of shale fabric are systematically reviewed for working fluids such as neutral fluids,acid fluids,alkali fluids and oxidative fluids.It is shown that shale is generally rich in water-sensitive components such as clay minerals,acidsensitive components(like carbonate minerals),alkali-sensitive components(like quartz),oxidative-sensitive components(like organic matter and pyrite),which easily lead to change of rock fabric and mechanical properties owing to water-rock interaction.According to the results,oxidizing acid fluids and oxidizing fracturing fluids should be used to enhance shale gas recovery.This study also indicates that an aspect playing an important role in increasing cumulative gas production is the optimization of the maximum shut-in time based on the change point of the wellhead pressure drop rate.Another important influential factor to be considered is the control of the wellhead pressure considering the stress sensitivity and creep characteristics of the fracture network.展开更多
Selenium(Se),as an important quasi-metal element,has attracted much attention in the fields of thin-film solar cells,electrocatalysts and energy storage applications,due to its unique physical and chemical properties....Selenium(Se),as an important quasi-metal element,has attracted much attention in the fields of thin-film solar cells,electrocatalysts and energy storage applications,due to its unique physical and chemical properties.However,the electrochemical behavior of Se in different systems from electrolytic cell to battery are complex and not fully understood.In this article,we focus on the electrochemical processes of Se in aqueous solutions,molten salts and ionic liquid electrolytes,as well as the application of Se-containing materials in energy storage.Initially,the electrochemical behaviors of Se-containing species in different systems are comprehensively summarized to understand the complexity of the kinetic processes and guide the Se electrodeposition.Then,the relationship between the deposition conditions and resulting structure and morphology of electrodeposited Se is discussed,so as to regulate the morphology and composition of the products.Finally,the advanced energy storage applications of Se in thin-film solar cells and secondary batteries are reviewed,and the electrochemical reaction processes of Se are systematically comprehended in monovalent and multivalent metal-ion batteries.Based on understanding the fundamental electrochemistry mechanism,the future development directions of Se-containing materials are considered in view of the in-depth review of reaction kinetics and energy storage applications.展开更多
The pivotal areas for the extensive and effective exploitation of shale gas in the Southern Sichuan Basin have recently transitioned from mid-deep layers to deep layers.Given challenges such as intricate data analysis...The pivotal areas for the extensive and effective exploitation of shale gas in the Southern Sichuan Basin have recently transitioned from mid-deep layers to deep layers.Given challenges such as intricate data analysis,absence of effective assessment methodologies,real-time control strategies,and scarce knowledge of the factors influencing deep gas wells in the so-called flowback stage,a comprehensive study was undertaken on over 160 deep gas wells in Luzhou block utilizing linear flow models and advanced big data analytics techniques.The research results show that:(1)The flowback stage of a deep gas well presents the characteristics of late gas channeling,high flowback rate after gas channeling,low 30-day flowback rate,and high flowback rate corresponding to peak production;(2)The comprehensive parameter AcmKm1/2 in the flowback stage exhibits a strong correlation with the Estimated Ultimate Recovery(EUR),allowing for the establishment of a standardized chart to evaluate EUR classification in typical shale gas wells during this stage.This enables quantitative assessment of gas well EUR,providing valuable insights into production potential and performance;(3)The spacing range and the initial productivity of gas wells have a significant impact on the overall effectiveness of gas wells.Therefore,it is crucial to further explore rational well patterns and spacing,as well as optimize initial drainage and production technical strategies in order to improve their performance.展开更多
This paper focuses on the design of fixtures for NP2 and NP4 cylinder heads on a horizontal machining center of flexible machining automatic lines.It began with an analysis of the diagrams of part processing and worki...This paper focuses on the design of fixtures for NP2 and NP4 cylinder heads on a horizontal machining center of flexible machining automatic lines.It began with an analysis of the diagrams of part processing and working procedure which formed the basis for the design of the processing technology scheme,a selection of suitable machine tools,and the setting of processing parameters.Fixtures tailored to the chosen machine tools were then designed to meet the processing requirements.Additional aspects of the project included the design of part drawings,calculation of working time quota,design of auxiliary guides,support clamping,and hydraulic circuits,all aimed at fulfilling practical production requirements.展开更多
[Objective] To study current development and utilization of water resources in Yuan'an County and countermeasures. [Method] On the basis of analyzing basic characteristics of water resources in Yuan'an County ...[Objective] To study current development and utilization of water resources in Yuan'an County and countermeasures. [Method] On the basis of analyzing basic characteristics of water resources in Yuan'an County as well as its current development and utilization, problems in the development and utilization of water resources in the study area were pointed out, and feasible countermeasures were proposed correspondingly. [Result] The county is endowed with rich water resources, but the overall use efficiency is low. There are many problems in the development and utilization of local water resources, such as pollution of water source, water and soil loss and so on. [Conclusion] In view of the problems, feasible suggestions were given to provide scientific support for the reasonable development and utilization of water resources in Yuan'an County.展开更多
基金supported by the Technical Project of Guangdong Province, China (Nos. 2020B090923002, 2021A1515011756)GDAS’ Project of Science and Technology Development, China (No. 2021GDASYL20210302006)+3 种基金Sciences Platform Environment and Capacity Building Projects of GDAS, China (No. 2021GDASYL-20210102005)Key R&D Program of Guangdong Province, China (No. 2020B090923002)Guangdong Special Support Program, China (No. 2019BT02C629)Guangdong Basic and Applied Basic Research Fund, China (Nos. 2020A1515111031, 2021A1515010939)。
基金supported by grants from the University Synergy Innovation Program of Anhui Province,China(GXXT-2021-058)the National Natural Science Foundation of China(U20A2033)+4 种基金the Natural Science Foundation of Anhui Province,China(2108085MC98)the Key Scientific and Technological Breakthroughs of Anhui Province,China(2021d06050003)the Anhui Province Education Department Sciences Research Project,China(YJS20210212)the Scientific Research Project of Higher Education in Anhui Province,China(2022AH050924 and 2022AH050885)the Jiangsu Collaborative Innovation Center for Modern Crop Production,China(JCIC-MCP)。
文摘The P-type plasma membrane(PM)H^(+)-ATPases(HAs)are crucial for plant development,growth,and defense.The HAs have been thoroughly characterized in many different plants.However,despite their importance,the functions of HAs in germination and seed dormancy(SD)have not been validated in wheat.Here,we identified 28 TaHA genes(TaHA1-28)in common wheat,which were divided into five subfamilies.An examination of gene expression in strong-and weak-SD wheat varieties led to the discovery of six candidate genes(TaHA7/-12/-14/-16/-18/-20).Based on a single nucleotide polymorphism(SNP)mutation(C/T)in the TaHA7 coding region,a CAPS marker(HA7)was developed and validated in 168 wheat varieties and 171 Chinese mini-core collections that exhibit diverse germination and SD phenotypes.We further verified the roles of the two allelic variations of TaHA7 in germination and SD using wheat mutants mutagenized with ethyl methane sulphonate(EMS)in‘Jimai 22’and‘Jing 411’backgrounds,and in transgenic Arabidopsis lines.TaHA7 appears to regulate germination and SD by mediating gibberellic acid(GA)and abscisic acid(ABA)signaling,metabolism,and biosynthesis.The results presented here will enable future research regarding the TaHAs in wheat.
基金the National Natural Science Foun-dation of China(Grant Nos.52376083 and 51991362).
文摘The heat transfer and stability of methane hydrate in reservoirs have a direct impact on the drilling and production efficiency of hydrate resources,especially in complex stress environments caused by formation subsidence.In this study,we investigated the thermal transport and structural stability of methane hydrate under triaxial compression using molecular dynamics simulations.The results suggest that the thermal conductivity of methane hydrate increases with increasing compression strain.Two phonon transport mechanisms were identified as factors enhancing thermal conductivity.At low compressive strains,a low-frequency phonon transport channel was established due to the overlap of phonon vibration peaks between methane and water molecules.At high compressive strains,the filling of larger phonon bandgaps facilitated the opening of more phonon transport channels.Additionally,we found that a strain of0.04 is a watershed point,where methane hydrate transitions from stable to unstable.Furthermore,a strain of0.06 marks the threshold at which the diffusion capacities of methane and water molecules are at their peaks.At a higher strain of0.08,the increased volume compression reduces the available space,limiting the diffusion ability of water and methane molecules within the hydrate.The synergistic effect of the strong diffusion ability and high probability of collision between atoms increases the thermal conductivity of hydrates during the unstable period compared to the stable period.Our findings offer valuable theoretical insights into the thermal conductivity and stability of methane hydrates in reservoir stress environments.
基金Lijun,You,Innovative Research Project for Sichuan Youth Scientific and Technological Innovation(Grants No.2016TD0016)Qiuyang Cheng,Postdoctoral Research Project of Petrochina Southwest Oil and Gas Field Company(Grants No.20230304-13).
文摘Horizontal well drilling and multi-stage hydraulic fracturing technologies are at the root of commercial shale gas development and exploitation.During these processes,typically,a large amount of working fluid enters the formation,resulting in widespread water-rock interaction.Deeply understanding such effects is required to optimize the production system.In this study,the mechanisms of water-rock interaction and the associated responses of shale fabric are systematically reviewed for working fluids such as neutral fluids,acid fluids,alkali fluids and oxidative fluids.It is shown that shale is generally rich in water-sensitive components such as clay minerals,acidsensitive components(like carbonate minerals),alkali-sensitive components(like quartz),oxidative-sensitive components(like organic matter and pyrite),which easily lead to change of rock fabric and mechanical properties owing to water-rock interaction.According to the results,oxidizing acid fluids and oxidizing fracturing fluids should be used to enhance shale gas recovery.This study also indicates that an aspect playing an important role in increasing cumulative gas production is the optimization of the maximum shut-in time based on the change point of the wellhead pressure drop rate.Another important influential factor to be considered is the control of the wellhead pressure considering the stress sensitivity and creep characteristics of the fracture network.
基金supported by the Fundamental Research Funds for the Central Universities(FRF-TP-19-079A1)National Natural Science Foundation of China(51804022,51725401)
文摘Selenium(Se),as an important quasi-metal element,has attracted much attention in the fields of thin-film solar cells,electrocatalysts and energy storage applications,due to its unique physical and chemical properties.However,the electrochemical behavior of Se in different systems from electrolytic cell to battery are complex and not fully understood.In this article,we focus on the electrochemical processes of Se in aqueous solutions,molten salts and ionic liquid electrolytes,as well as the application of Se-containing materials in energy storage.Initially,the electrochemical behaviors of Se-containing species in different systems are comprehensively summarized to understand the complexity of the kinetic processes and guide the Se electrodeposition.Then,the relationship between the deposition conditions and resulting structure and morphology of electrodeposited Se is discussed,so as to regulate the morphology and composition of the products.Finally,the advanced energy storage applications of Se in thin-film solar cells and secondary batteries are reviewed,and the electrochemical reaction processes of Se are systematically comprehended in monovalent and multivalent metal-ion batteries.Based on understanding the fundamental electrochemistry mechanism,the future development directions of Se-containing materials are considered in view of the in-depth review of reaction kinetics and energy storage applications.
文摘The pivotal areas for the extensive and effective exploitation of shale gas in the Southern Sichuan Basin have recently transitioned from mid-deep layers to deep layers.Given challenges such as intricate data analysis,absence of effective assessment methodologies,real-time control strategies,and scarce knowledge of the factors influencing deep gas wells in the so-called flowback stage,a comprehensive study was undertaken on over 160 deep gas wells in Luzhou block utilizing linear flow models and advanced big data analytics techniques.The research results show that:(1)The flowback stage of a deep gas well presents the characteristics of late gas channeling,high flowback rate after gas channeling,low 30-day flowback rate,and high flowback rate corresponding to peak production;(2)The comprehensive parameter AcmKm1/2 in the flowback stage exhibits a strong correlation with the Estimated Ultimate Recovery(EUR),allowing for the establishment of a standardized chart to evaluate EUR classification in typical shale gas wells during this stage.This enables quantitative assessment of gas well EUR,providing valuable insights into production potential and performance;(3)The spacing range and the initial productivity of gas wells have a significant impact on the overall effectiveness of gas wells.Therefore,it is crucial to further explore rational well patterns and spacing,as well as optimize initial drainage and production technical strategies in order to improve their performance.
文摘This paper focuses on the design of fixtures for NP2 and NP4 cylinder heads on a horizontal machining center of flexible machining automatic lines.It began with an analysis of the diagrams of part processing and working procedure which formed the basis for the design of the processing technology scheme,a selection of suitable machine tools,and the setting of processing parameters.Fixtures tailored to the chosen machine tools were then designed to meet the processing requirements.Additional aspects of the project included the design of part drawings,calculation of working time quota,design of auxiliary guides,support clamping,and hydraulic circuits,all aimed at fulfilling practical production requirements.
文摘[Objective] To study current development and utilization of water resources in Yuan'an County and countermeasures. [Method] On the basis of analyzing basic characteristics of water resources in Yuan'an County as well as its current development and utilization, problems in the development and utilization of water resources in the study area were pointed out, and feasible countermeasures were proposed correspondingly. [Result] The county is endowed with rich water resources, but the overall use efficiency is low. There are many problems in the development and utilization of local water resources, such as pollution of water source, water and soil loss and so on. [Conclusion] In view of the problems, feasible suggestions were given to provide scientific support for the reasonable development and utilization of water resources in Yuan'an County.
