GC-MS Analysis of Volatile Oils in Mango Leaves from Different Producing Areas

[Objectives]To analyze the composition of volatile oils from mango leaves from different producing areas.[Methods]The volatile oils in mango leaves from different areas was extracted by steam distillation,and the GC-MS method combined with retention index and peak area normalization method was used for qualitative and quantitative analysis.[Results]The volatile oils of mango leaves in southern provinces contained high content of terpenes,such asα-gurjunene(18.39%-34.13%),(+)-viridiflorene(8.95%-16.30%)and(E)-β-ocimene(3.49%-7.63%).The volatile oils components of mango leaves were significantly different in different provinces,for example,mango leaves produced in Hainan Province contained a large amount ofβ-selinene(23.00%),but not in other provinces.[Conclusions]The origin had a great influence on the composition and contents of volatile oils in mango leaves.

Determination of Mangiferin and Homomangiferin in the Leaves of 93 Kinds of Mango

[Objectives]To determine the content of mangiferin and homomangiferin in mango leaves by HPLC.[Methods]The mangiferin and homomangiferin were separated and determined by Elite Hypersil C18(5μm,4.6 mm ID×250 mm)chromatographic column.Acetonitrile-0.1%(V/V)phosphoric acid solution was used as the mobile phase for gradient elution,the flow rate was 1.0 mL/min,the detection wavelength was 258 nm,the column temperature was 30℃,and the injection volume was 5μL.[Results]There was a good linear relationship between mangiferin and homomangiferin in the range of 0.0254-0.5080μg/μL(r=0.9999)and 0.000960-0.019200μg/μL(r=0.9999),respectively.The average recovery rate(n=6)of mangiferin and homomangiferin in mango leaves was 101.7%(RSD=2.0%)and 101.0%(RSD=1.7%),respectively.[Conclusions]There were great differences in the content of mangiferin and homomangiferin in the leaves of different varieties of mango.The experimental results could provide a scientific basis for further development and utilization of mango leaf resources.

Explorations in Experimental Teaching of Traditional Chinese Medicine Instrumental Analysis Based on the Training of Innovative Talents

Training students’innovative thinking is an essential task of talent training in colleges and universities,and how to train students’innovativeness in experimental teaching is the focus of modern education.Experimental lesson of instrumental analysis is an important section for integrating students’theories and practices,in view of the characteristics of traditional Chinese medicine major and instrumental analysis lesson,this lesson orientates opening up students’innovative thinking,explores the way of improving students’innovative ability in an all-around way through reforming the experimental teaching of instrumental analysis according to the teaching objectives.

Quality Standard of Rutin in the Leaves of Thespesia populnea

[Objectives]To establish a quality standard method for detecting rutin in leaves of Thespesia populnea.[Methods]The leaves of T.populnea as a reagent,the rutin in the leaves of T.populnea was extracted by alcohol extraction,and quantitatively analyzed by high performance liquid chromatography(HPLC).[Results]In the range of 0.21-3.36μg,the peak area A of rutin in the leaves of T.populnea had a good linear relationship with its injection volume m.A=1561.6 m-6.893,and the correlation coefficient r=1.0000.The method was applied to the determination of rutin ethyl acetate extract in the leaves of T.populnea,the average recovery rate was 101.75%,and the RSD was 1.32%.[Conclusions]This method is simple and feasible,the results are accurate,the repeatability is good,and the separation effect is good.It is expected to provide a scientific idea for the determination of rutin in the leaves of T.populnea.

Analysis of Volatile Constituents in Agrimonia pilosa Ledeb from Different Origins by GC-MS

[Objective]This study aimed to analyze the chemical composition of volatile oils in Agrimonia pilosa Ledeb.from different origins.[Method]HP-5MS capillary columns were used and column temperature was controlled by a program.MS analysis was performed with EI and quadruple mass analyzer.The chemical composition was identified by NIST02 and Wiley275 library,and the relative contents were determined with chromatographic peak area normalization method.[Result]According to GC-MS total ion-current chromatograms,41 chromatographic peaks of volatile constituents in A.pilosa from Zhejiang Province were separated,and 27 chemical compounds were identified,mainly including palmitic acid,cedrol and l-bornyl acetate;47 chromatographic peaks of volatile constituents in A.pilosa from Hunan Province were separated,and 27 chemical compounds were identified,mainly including palmitic acid,（-）-a-selinene andβ-bisabolene;22chromatographic peaks of volatile constituents in A.pilosa from Guangxi Zhuang Autonomous Region were separated,and 13 chemical compounds were identified,mainly including palmitic acid,linoleic acid and caryophyllene oxide.Volatile constituents in A.pilosa from Zhejiang Province and Hunan Province exhibited similar species and contents with several unique compounds,and volatile oils in A.pilosa from these two origins varied significantly from volatile oils in A.pilosa from Guangxi Zhuang Autonomous Region.[Conclusion]This study provided an important basis for the development and utilization of volatile oils in A.pilosa.