High-throughput screening system of citrus bacterial cankerassociated transcription factors and its application to the regulation of citrus canker resistance

One of the main diseases that adversely impacts the global citrus industry is citrus bacterial canker(CBC),caused by the bacteria Xanthomonas citri subsp.citri(Xcc).Response to CBC is a complex process,with both proteinDNA as well as protein–protein interactions for the regulatory network.To detect such interactions in CBC resistant regulation,a citrus high-throughput screening system with 203 CBC-inducible transcription factors(TFs),were developed.Screening the upstream regulators of target by yeast-one hybrid(Y1H)methods was also performed.A regulatory module of CBC resistance was identified based on this system.One TF(CsDOF5.8)was explored due to its interactions with the 1-kb promoter fragment of CsPrx25,a resistant gene of CBC involved in reactive oxygen species(ROS)homeostasis regulation.Electrophoretic mobility shift assay(EMSA),dual-LUC assays,as well as transient overexpression of CsDOF5.8,further validated the interactions and transcriptional regulation.The CsDOF5.8–CsPrx25 promoter interaction revealed a complex pathway that governs the regulation of CBC resistance via H2O2homeostasis.The high-throughput Y1H/Y2H screening system could be an efficient tool for studying regulatory pathways or network of CBC resistance regulation.In addition,it could highlight the potential of these candidate genes as targets for efforts to breed CBC-resistant citrus varieties.

Systematic analysis and functional verification of citrus glutathione S-transferases reveals that CsGSTF1 and CsGSTU18contribute negatively to citrus bacterial canker

Citrus bacterial canker(CBC) is resulted from Xanthomonas citri subsp. citri(Xcc) infection and poses a significant threat to citrus production.Glutathione S-transferases(GSTs) are critical in maintaining redox homeostasis in plants, especially in relation to abiotic and biotic stress responses. However, the function of GSTs in resisting CBC remains unclear. Here, citrus glutathione S-transferases were investigated applying a genome-wide approach. In total, 69 CsGSTs belonging to seven classes were identified, and the phylogeny, chromosomal distribution, gene structures and conserved motifs were analyzed. Several CsGSTs responded to Xcc infection, as observed in the upregulation of CsGSTF1 and CsGSTU18 in the CBC-sensitive ‘Wanjincheng' variety but not in the resistant ‘Kumquat' variety. CsGSTF1 and CsGSTU18 were localized at the cytoplasm. Transient overexpression of CsGSTF1 and CsGSTU18 mediated reactive oxygen species(ROS) scavenging, whereas the virus-induced gene silencing(VIGS) of CsGSTF1 and CsGSTU18 caused strong CBC resistance and ROS burst. The present study investigated the characterization of citrus GST gene family, and discovered that CsGSTF1 and CsGSTU18 negatively contributed to CBC through modulating ROS homeostasis. These findings emphasize the significance of GSTs in infection resistance in plants.

Use of evergreen and deciduous plants by nocturnal-roosting birds:A case study in Beijing

With continually increasing urbanization,the land cover in urban areas continues to change,resulting in the loss of biodiversity.Birds are highly sensitive to changes in habitat.Most forest birds perch on plants that provide increased safety to reduce the risk of predation,and small birds may also consider insulation when using roosting plants in winter because of cold weather.Landscaping plants thus shape the nocturnal roosting environment of urban birds,and proper planting is essential for the survival of birds at night.The use of roosting plants by urban birds should therefore be studied to provide a reference for landscaping.In the current study,we observed 1865 nocturnal roosting birds in Beijing from 2021 to 2022,with 23 species of birds from 12 families and 45 species of plants from 22 families recorded.Juniperus chinensis exhibited the highest bird rarity-weighted richness,followed by Fraxinus pennsylvanica,Phyllostachys propinqua,Pinus tabuliformis,and Ulmus pumila.The diameter at breast height,tree height,and crown width of plants used by birds was largest in summer and smallest in winter,and the perch height of birds was the highest in spring and summer and the lowest in winter.Birds used the highest proportion of deciduous plants in summer and the highest proportion of evergreen plants in winter.A significant seasonal difference in the use of evergreen and deciduous plants by small birds was noted,with a preference for deciduous plants in summer and evergreen plants in winter,while this preference was not found in large birds.These findings indicate that evergreen plants provide a vital nocturnal roosting environment for small birds in winter.To provide a better nocturnal roosting habitat for urban birds,we recommend paying attention to the combination of evergreen and deciduous plants when carrying out landscape construction.

Baseline symptoms of depression and anxiety negatively impact the effectiveness of CBTi in treating acute insomnia among young adults

Background Cognitive-behavioural therapy for insomnia(CBTi)is the first-line treatment for those with this sleep disorder.However,depressive and anxiety symptoms often co-occur with acute insomnia,which may affect the effectiveness of CBTitreatment.Aims This study aimed to determine the impact of depressive and anxiety symptoms on the efficacy of CBTi in treating acute insomnia.Methods A single-arm clinical trial was conducted among individuals who have acute insomnia.Participants underwent self-guided CBTi for 1-week.Their insomnia,depressive symptoms and anxiety symptoms were evaluated using the Insomnia Severity Index and the Hospital Anxiety and Depression Scale at baseline,post-treatment and 3-month follow-up.Repeated measures analysis of variance was used to assess the effectiveness of CBTi in treating insomnia,depressive symptoms and anxiety symptoms.A multivariate Cox regression model was used to determine the impact of depressive and anxiety symptoms on insomnia.Results The study found significant reductions in insomnia,depressive symptoms and anxiety symptoms at both post-treatment and 3-month follow-up(F=17.45,p<0.001;F=36.37,p=0.001;and F=81.51,p<0.001,respectively).The duration of CBTi treatment had a positive impact on insomnia recovery(hazard ratio(HR)=0.94,p=0.018).However,baseline depressive symptoms(HR=1.83,p=0.004)and baseline anxiety symptoms(HR=1.99,p=0.001)had significant negative effects on insomnia recovery.Conclusions The study showed that a 1-week self-guided CBTi treatment is effective in treating acute insomnia and comorbid depressive and anxiety symptoms.However,baseline depressive and anxiety symptoms negatively impact treatment effectiveness.Therefore,clinicians should assess for depressive and anxiety symptoms before treating acute insomnia with monotherapy CBTi.

基于STEM理念的案例式混合教学法——以“应用化学实验”课程为例

将STEM(Science,Technology,Engineering,Mathematics)教学理念融入应用化学实验的SPOC(Small Private Online Course)线上线下混合式教学,采用针对不同实验项目的案例式教学法,学生课前完成实验讲义和视频的线上预习,线下针对案例问题,综合运用多学科知识分组讨论,在教师指导下解决问题并提出实验方案,进行实验操作,在课后进行STEM教学评价。以上教学手段的实践将线上教学资源和线下实验操作有机融合,促进学生自主学习,实现对学生科学精神与合作创新能力的培养。

Intelligent method to experimentally identify the fracture mechanism of red sandstone

Tensile and shear fractures are significant mechanisms for rock failure.Understanding the fractures that occur in rock can reveal rock failure mechanisms.Scanning electron microscopy(SEM)has been widely used to analyze tensile and shear fractures of rock on a mesoscopic scale.To quantify tensile and shear fractures,this study proposed an innovative method composed of SEM images and deep learning techniques to identify tensile and shear fractures in red sandstone.First,direct tensile and preset angle shear tests were performed for red sandstone to produce representative tensile and shear fracture surfaces,which were then observed by SEM.Second,these obtained SEM images were applied to develop deep learning models(AlexNet,VGG13,and SqueezeNet).Model evaluation showed that VGG13 was the best model,with a testing accuracy of 0.985.Third,the features of tensile and shear fractures of red sandstone learned by VGG13 were analyzed by the integrated gradient algorithm.VGG13 was then implemented to identify the distribution and proportion of tensile and shear fractures on the failure surfaces of rock fragments caused by uniaxial compression and Brazilian splitting tests.Results demonstrated the model feasibility and suggested that the proposed method can reveal rock failure mechanisms.

CsBZIP40 confers resistance against citrus bacterial canker by repressing CsWRKY43-CsPrx53/CsSOD13 cascade mediated ROS scavenging

As the bacterial etiologic agent causing citrus bacterial canker(CBC),Xanthomonas citri subsp.citri(Xcc)seriously impacts citrus plantation and fruit production globally.In an earlier study,we demonstrated that CsBZIP40 can positively impact CBC resistance in the sweet orange(Citrus sinensis).However,the mechanistic basis for the protective benefits conferred by CsBZIP40 is yet to be delineated.Here,we show that CsBZIP40 positively regulates CBC resistance and reactive oxygen species(ROS)homeostasis in transgenic sweet orange overexpressing CsBZIP40.CsBZIP40 directly binds to the TGA-box of the CsWRKY43 promoter to repress its transcriptional activity.CsWRKY43 overexpression induces CBC susceptibility in transgenic sweet oranges.In contrast,its inhibition produces strong resistance to CBC.CsWRKY43 directly binds to the W-boxes of the CsPrx53 and CsSOD13 promoters to positively regulate the activities of these antioxidant enzymes,resulting in the negative regulation of ROS homeostasis and CBC resistance in sweet orange plants.CsPrx53/CsSOD13 knockdown enhances ROS accumulation and CBC resistance.Overall,our results outline a regulatory pathway through which CsBZIP40 transcriptionally represses CsWRKY43-CsPrx53/CsSOD13 cascade-mediated ROS scavenging in a manner conducive to CBC resistance.These mechanisms underscore the potential importance of CsBZIP40,CsWRKY43,CsPrx53,and CsSOD13,providing promising strategies for the prevention of CBC.

关于Arrhenius公式的几点讨论

Arrhenius公式是最重要的化学动力学经验公式,提出百余年来,科学家对其的讨论从未停止。本文溯源了该公式产生的历史过程,进一步说明其与van’tHoff方程的联系。针对普遍接受的Tolman活化能的物理意义进行了讨论,给出了修正建议。对活化能随温度变化的情况进行了讨论,对Arrhenius公式的适用温度范围进行了量化说明,建议对于分子结构较复杂的反应,即便在通常温度范围内,亦应采用三常数公式。对物理化学教材中与Arrhenius公式具有相似形式的公式进行了概括,说明了其相似性的本质。相关讨论有助于师生更正确地理解和使用Arrhenius公式。

Three-dimensionally ordered macroporous perovskite materials for environmental applications

Three-dimensionally ordered macroporous(3DOM)perovskite materials have attracted the interest from researchers worldwide due to their unique macroporous structure,flexible composition,tailorable physicochemical property,high stability and biocompatibility.In particular,they were widely used in environmental field,such as photocatalysis,catalytic combustion,catalytic oxidation and sensors.In this review,the recent progresses in the synthesis of 3DOM perovskite materials and their environmental applications are summarized.The advantages and the promoting mechanisms of 3DOM perovskite materials for different applications are discussed in detail.Subsequently,the challenges and perspectives on the topic are proposed.

Effective Improvement of the Photovoltaic Performance of Carbon-Based Perovskite Solar Cells by Additional Solvents

A solvent-assisted methodology has been developed to synthesize CH_3NH_3 PbI_3perovskite absorber layers.It involved the use of a mixed solvent of CH_3NH_3 I,PbI_2,c-butyrolactone,and dimethyl sulfoxide(DMSO) followed by the addition of chlorobenzene(CB).The method produced ultra-flat and dense perovskite capping layers atop mesoporous TiO_2 films,enabling a remarkable improvement in the performance of free hole transport material(HTM) carbon electrode-based perovskite solar cells(PSCs).Toluene(TO) was also studied as an additional solvent for comparison.At the annealing temperature of 100 °C,the fabricated HTM-free PSCs based on drop-casting CB demonstrated power conversion efficiency(PCE) of 9.73 %,which is 36 and 71 % higher than those fabricated from the perovskite films using TO or without adding an extra solvent,respectively.The interaction between the PbI_2–DMSO–CH_3NH_3I intermediate phase and the additional solvent was discussed.Furthermore,the influence of the annealing temperature on the absorber film formation,morphology,and crystalline structure was investigated and correlated with the photovoltaic performance.Highly efficient,simple,and stable HTM-free solar cells with a PCE of 11.44 % were prepared utilizing the optimum perovskite absorbers annealed at 120 °C.

Double cores of the Ozone Low in the vertical direction over the Asian continent in satellite data sets

Using four satellite data sets(TOMS/SBUV, OMI, MLS, and HALOE), we analyze the seasonal variations of the total column ozone(TCO) and its zonal deviation(TCO*), and reveal the vertical structure of the Ozone Low(OV) over the Asian continent. Our principal findings are:(1) The TCO over the Asian continent reaches its maximum in the spring and its minimum in the autumn. The Ozone Low exists from May to September.(2) The Ozone Low has two negative cores, located in the lower and the upper stratosphere. The lower core is near 30 hPa in the winter and 70 hPa in the other seasons. The upper core varies from 10 hPa to 1 hPa among the four seasons.(3)The position of the Ozone Low in the lower and the upper stratosphere over the Asian continent shows seasonal variability.

Intrusion Detection Algorithm Based on Density,Cluster Centers,and Nearest Neighbors

Intrusion detection aims to detect intrusion behavior and serves as a complement to firewalls.It can detect attack types of malicious network communications and computer usage that cannot be detected by idiomatic firewalls.Many intrusion detection methods are processed through machine learning.Previous literature has shown that the performance of an intrusion detection method based on hybrid learning or integration approach is superior to that of single learning technology.However,almost no studies focus on how additional representative and concise features can be extracted to process effective intrusion detection among massive and complicated data.In this paper,a new hybrid learning method is proposed on the basis of features such as density,cluster centers,and nearest neighbors(DCNN).In this algorithm,data is represented by the local density of each sample point and the sum of distances from each sample point to cluster centers and to its nearest neighbor.k-NN classifier is adopted to classify the new feature vectors.Our experiment shows that DCNN,which combines K-means,clustering-based density,and k-NN classifier,is effective in intrusion detection.

CLEAN:Frequent Pattern-Based Trajectory Compression and Computation on Road Networks

The volume of trajectory data has become tremendously huge in recent years. How to effectively and efficiently maintain and compute such trajectory data has become a challenging task. In this paper, we propose a trajectory spatial and temporal compression framework, namely CLEAN. The key of spatial compression is to mine meaningful trajectory frequent patterns on road network. By treating the mined patterns as dictionary items, the long trajectories have the chance to be encoded by shorter paths, thus leading to smaller space cost. And an error-bounded temporal compression is carefully designed on top of the identified spatial patterns for much low space cost. Meanwhile, the patterns are also utilized to improve the performance of two trajectory applications, range query and clustering, without decompression overhead. Extensive experiments on real trajectory datasets validate that CLEAN significantly outperforms existing state-of-art approaches in terms of spatial-temporal compression and trajectory applications.

Application of New Modified Bentonite in Decolorization of Simulated Printing and Dyeing Wastewater

The problem of colority control of printing and dyeing wastewater has become a technical problem that plagues related companies.Bentonite is an adsorbent with excellent properties.Modification of bentonite with environmentally friendly substances can improve its decolorization performance.In this experiment,the simulated printing and dyeing wastewater was taken as the control object,and the bentonite was modified with environmentally friendly materials such as sodium carboxymethyl cellulose(CMC)and lignin to prepare a new modified bentonite;then the modified bentonite was used to adsorb the simulated wastewater to reduce the water colority and COD;finally the relevant design of the adsorption process was made.Results indicate that M lignin∶M CMC∶M original bentonite=1∶2∶97 had the optimum treatment effect,the optimum modification temperature was 30℃ and the modification time was 4 h;the optimum conditions for the adsorption process were:pH=5,temperature=30℃,reaction time=60 min,dosage=0.05 g of modified bentonite/mL simulated dye solution.The final removal rate of colority and COD reached 95.0% and 98.2%,respectively.Compared with the original bentonite,this new modified bentonite has greater adsorption capacity and thus has greater application value.

异银杏素对人肝癌Huh7细胞生物学行为的影响

目的:研究异银杏素对人肝癌Huh7细胞生物学行为的影响。方法:不同浓度的异银杏素对Huh7细胞处理不同的时间,采用CCK-8法检测细胞增殖,划痕实验和Transwell法检测细胞迁移和侵袭,流式细胞术检测细胞凋亡和细胞周期。结果:异银杏素浓度依赖性地抑制Huh7细胞的增殖(IC50=16.68μmol/L,48 h;IC50=15.42μmol/L,96 h)。经异银杏素处理24 h后的细胞,划痕愈合率和细胞侵袭率显著降低;流式细胞术结果显示,12.5μmol/L异银杏素实验组细胞阻滞在G0/G1期,且各实验组细胞凋亡率均有所上升。结论:异银杏素能抑制Huh7细胞增殖、迁移和侵袭,并改变细胞周期分布,诱导细胞凋亡。

Analysis of bearing capacity of pile foundation in discontinued permafrost regions

Piles are the main building foundation in permafrost regions. Thawing the permafrost foundation would have a negative effect on a pile, and may cause damage to the building. This paper focuses on the effects of negative friction force due to the melt of permafrost, and presents four calculated methods for bearing capacity of a pile. An engineering station was taken as an example, where the lengths of a pile were compared based on four methods. Finally, quick field load tests were carried out, and some meaningful conclusions are presented. Thus, these analytical results can be used to design a pile for permafrost regions.

Spatial Distribution Characteristics of Heavy Metals Cu and Zn in Coastal Wetland Sediments

The content of Cu,Zn in the sediments from coastal wetlands of the Yellow River Delta was determined.The results showed that:(i)The content of Cu,Zn range was 16.70-50.40 mg/kg,18.15-48.80 mg/kg,respectively.The mean content of Cu,Zn was 31.12mg/kg,36.74 mg/kg,respectively.Compared with the soil environmental background values of Shandong Province,the content of Cu was excessive,while the content of Zn was below the background.(ii)The concentrations of Cu,Zn in sediment in the coast of the Yellow River Delta were higher than in the other two areas.(iii)Vertical distribution characteristics of the concentrations of Cu,Zn were increasing with depth.The maximum content of Cu,Zn was 80-100 cm,in general,the content of Cu,Zn in the bottom sediments was higher than that in the surface sediments.The growth and decline trends of Zn in Tamarix and Suaeda areas were much the same and all higher than in the Phragmites communis area.(iv)The distribution characteristics of Cu concentration in different vegetation cover in sediment is Suaeda>Phragmites communis>Tamarix,which reflected different effects on retention of Cu in the sediments because of the different vegetation types.The highest content of Zn in the 0-20 cm sediments in Tamarix area was 44.07 mg/kg.

Dual-microcomb generation via a monochromatically pumped dual-mode microresonator

Microcombs have enabled a host of cutting-edge applications from metrology to communications that have garnered significant attention in the last decade.Nevertheless,due to the thermal instability of the microresonator,additional control devices like auxiliary lasers are indispensable for single-soliton generation in some scenarios.Specifically,the increased system complexity would be too overwhelming for dual-microcomb generation.Here,we put forward a novel approach to mitigate the thermal instability and generate the dual-microcomb using a compact system.This process is akin to mode-division multiplexing,as the dual-microcombs are generated by pumping the dual-mode of a single Si_(3)N_(4) microresonator with a continuous-wave laser.Both numerical simulations and experimental measurements indicate that this innovative technique could offer a straightforward way to enlarge the soliton existence range,allowing entry into the multistability regime and triggering another microcomb alongside the main soliton pulse.This outcome not only shines new light on the interaction mechanism of microresonator modes but also provides an avenue for the development of dual-microcomb-based ranging and low phase noise microwave generation.

Segregation of binary particles in gas-solid fluidized bed

Particle segregation and mixing behavior play a crucial role in industrial processes.This study investigates the saturated jetsam fraction,which indicates the maximum capacity of flotsam to entrain jetsam,in an initially separated binary fluidized bed with particle size differences.According to the value of saturated jetsam fraction,three distinct regimes-segregation,mixing,and an intermediate regime-are identified.Moreover,intriguing relationships between the saturated jetsam fraction and superficial gas velocity are observed,exhibiting monotonic trends in both the segregation and mixing regimes,while a unique volcano-shaped curve in the intermediate regime.Additionally,a comprehensive entrainment model based on two-fluid model elucidates the observed phenomena,emphasizing the significance of mixing behavior in fluidized layer on the saturated jetsam fraction.This work offers potential insights for evaluating segregation in industrial applications.

Comparative study on pump frequency tuning and self-injection locking in Kerr microcomb generation

The optical frequency comb has attracted considerable interest due to its diverse applications in optical atomic clocks,ultra-low-noise microwave generation,dual-comb spectroscopy,and optical communications.The merits of large frequency spacing,high integration,and low power consumption have shown that microresonator-based Kerr optical frequency combs will become mainstream in the future.Two methods of pump frequency tuning and self-injection locking were used to obtain Kerr combs in the same silicon nitride microresonators with free spectral ranges of 50 GHz and 100 GHz.Singlesoliton combs are realized with both methods.Simplicity,pump power,spectrum bandwidth,conversion efficiency,and linewidth are compared and analyzed.Our results show that the advantages of pump frequency tuning are a wider spectrum and higher soliton power while the advantages of self-injection locking are simplicity,compactness,low cost,significant linewidth narrowing,and high conversion efficiency.