Effect of RBM10 on regulating the proliferation and metastasis activity of human hepatocellular carcinoma cells by affecting the stability of miR-21

In recent decades,RNA binding motif(RBM)proteins have been widespread concerned by researchers.Among them,RBM5 is considered as a potential tumor suppressor gene in HCC.RBM10,also belonging to the RBM family,have similar structure and high homology with RBM5,indicating its potential as potential tumor suppressor genes.However,the role of RBM10 in tumors is controversial.The purpose of this study was to analyze the expression correlation and functional relationship of miR-21 and RBM10 in human hepatocellular carcinoma(HCC)tissues and corresponding tumor cells.Bioinformatics analysis showed that miR-21 and RBM10 were both highly expressed in HCC;similarly,the expression levels of miR-21 and RBM10 in HCC cells were significantly higher than those in normal hepatocytes.There was a positive correlation between miR-21 and RBM10.Furthermore,knockdown of RBM10 inhibited the proliferation,migration and invasion of SNU-398 cells,and simultaneous overexpression of miR-21 attenuated this inhibitory effect.Meanwhile,overexpressing RBM10 could promote the proliferation,migration and invasion of Hep G2 cells;while the stability of miR-21 could be reduced by knocking down RBM10.On the whole,the findings of this study indicate that RBM10 is involved in regulating the function and activity of human HCC cells by affecting the stability of miR-21.RBM10 cannot be simply summarized as a tumor suppressor or oncogene.Further studies are needed to clarify the role of RBM10 in tumors.

绿色电力与煤化工耦合路径分析

介绍了煤化工、绿电、绿氢现状,分析了绿色电力与煤化工耦合的必要性及存在的问题,并以煤制甲醇为例提出建议方案,旨在为政策制定者、行业决策者及环境保护倡导者提供参考,共同推动化工行业向更加绿色、高效、可持续的方向发展。

Research on Equipment Management System Based on Robot Laboratory

With the expansion of the scale of our hospital robot laboratory, equipment use, reserves are increasing, manual management equipment information has been unable to adapt to daily management, such as data record error rate increased, the number of experiments increased, equipment loss and maintenance is not clearly defined. In order to ensure the safety and accuracy of the equipment, a system of equipment management systems was developed as a result of the conversion to the use of management systems for the recording and storage of information. The design of this system is a perfect robot laboratory equipment management system based on the development of web framework technology for equipment management functions. The back end of this system uses the Java language, the SSM framework as the main body, the database is built by MySQL relational database, the front-end page uses JSP, Bootstrap, jQuery and other technologies, and uses Eclipse project construction tools for system development. After a period of trial operation, the level of laboratory equipment management has been significantly improved.

Research on Factors of Linear Algebra Learning Effect

Linear algebra has a very important application in physics and technical disciplines. This article conducted a questionnaire survey on the factors that affect the effect of linear algebra learning;the questionnaire contains several aspects of learning attitude, learning interest, learning methods, teaching methods, etc.;based on recycling data, cross chi-square test and multiple logistic regression analysis are used to obtain the factors that affect the effect of linear algebra learning. The research results show that: learning methods, learning attitudes, teaching methods and elementary algebra basics are the main factors that affect the learning effect of linear algebra;among them, there are positive correlations between teaching methods, learning methods, learning attitudes and learning effects;teaching methods, learning methods 3. The three principal components of learning attitude are positively correlated. Based on the research and analysis, the following conclusions are drawn: finding a suitable learning method for the college students and maintaining a positive learning attitude are effective means to improve the linear algebra learning effect of the college students;in teaching, it is recommended to advance with the times, the teaching content and teaching methods innovate to stimulate students’ interest in learning, thus improving the learning effect of college students’ linear algebra courses.

The Approximation of Hermite Interpolation on the Weighted Mean Norm

We research the simultaneous approximation problem of the higher-order Hermite interpolation based on the zeros of the second Chebyshev polynomials under weighted Lp-norm. The estimation is sharp.

About the Optimized Design of the Parking Space on the Campus of a College

In recent years, with the large increase in the number of motor vehicles in colleges and universities and the lag in campus planning, the relative shortage of parking spaces on campus has become increasingly serious. Taking Baoding College as an example, this article analyzes the current situation of static traffic on campus and finds out the problem of parking on campus through questionnaire surveys and field surveys. Analyze the growth trend of the number of motor vehicles based on the data, use the GM (1, 1) model and the linear fitting model to predict the number of motor vehicles in the future, and determine the size and layout of the parking lot based on the campus size, functional zoning, and road layout. The big campus-based parking system planning method based on big data can effectively solve the problems of small sample data, low accuracy, and poor timeliness of traditional methods, which improves the practicability and scientificity of planning results.

Conserved and differentiated functions of CIK receptor kinases in modulating stem cell signaling in Arabidopsis

The shoot apical meristem(SAM)and root apical meristem(RAM)act as pools of stem cells that give rise to aboveground and underground tissues and organs in higher plants,respectively.The CLAVATA3(CLV3)-WUSCHEL(WUS)negative-feedback loop acts as a core pathway controlling SAM homeostasis,while CLV3/EMBRYO SURROUNDING REGION(ESR)40(CLE40)and WUSCHEL-RELATED HOMEOBOX5(WOX5),homologs of CLV3 and WUS,direct columella stem cell fate.Moreover,CLV3 INSENSITIVE KINASES(CIKs)have been shown to be essential for maintaining SAM homeostasis,whereas whether they regulate the distal root meristem remains to be elucidated.Here,we report that CIKs are indispensable for transducing the CLE40 signal to maintain homeostasis of the distal root meristem.We found that the cik mutant roots displayed disrupted quiescent center and delayed columella stem cell(CSC)differentiation.Biochemical assays demonstrated that CIKs interact with ARABIDOPSIS CRINKLY4(ACR4)in a ligand-independent manner and can be phosphorylated by ACR4 in vitro.In addition,the phosphorylation of CIKs can be rapidly induced by CLE40,which partially depends on ACR4.Although CIKs act as conserved and redundant regulators in the SAM and RAM,our results demonstrated that they exhibit differentiated functions in these meristems.

Receptor-like protein kinase-mediated signaling in controlling root meristem homeostasis

Generation of the root greatly benefits higher plants living on land.Continuous root growth and development are achieved by the root apical meristem,which acts as a reservoir of stem cells.The stem cells,on the one hand,constantly renew themselves through cell division.On the other hand,they differentiate into functional cells to form diverse tissues of the root.The balance between the maintenance and consumption of the root apical meristem is governed by cell-to-cell communications.Receptor-like protein kinases(RLKs),a group of signaling molecules localized on the cell surface,have been implicated in sensing multiple endogenous and environmental signals for plant development and stress adaptation.Over the past two decades,various RLKs and their ligands have been revealed to participate in regulating root meristem homeostasis.In this review,we focus on the recent studies about RLK-mediated signaling in regulating the maintenance and consumption of the root apical meristem.

Versatile optical manipulation of trions,dark excitons and biexcitons through contrasting exciton-photon coupling

Various exciton species in transition metal dichalcogenides(TMDs),such as neutral excitons,trions(charged excitons),dark excitons,and biexcitons,have been individually discovered with distinct light-matter interactions.In terms of valley-spin locked band structures and electron-hole configurations,these exciton species demonstrate flexible control of emission light with degrees of freedom(DOFs)such as intensity,polarization,frequency,and dynamics.However,it remains elusive to fully manipulate different exciton species on demand for practical photonic applications.Here,we investigate the contrasting light-matter interactions to control multiple DOFs of emission light in a hybrid monolayer WSe2-Ag nanowire(NW)structure by taking advantage of various exciton species.These excitons,including trions,dark excitons,and biexcitons,are found to couple independently with propagating surface plasmon polaritons(SPPs)of Ag NW in quite different ways,thanks to the orientations of transition dipoles.Consistent with the simulations,the dark excitons and dark trions show extremely high coupling efficiency with SPPs,while the trions demonstrate directional chiral-coupling features.This study presents a crucial step towards the ultimate goal of exploiting the comprehensive spectrum of TMD excitons for optical information processing and quantum optics.

Shape change of calcite single crystals to accommodate interfacial curvature:Crystallization in presence of Mg2+ions and agarose gel-networks

Synthetic calcite single crystals,due to their strong crystal habit,tend to grow into characteristic rhombohedra.In the nature,biogenic calcite crystals form composites together with biomacromolecular materials,spurring investigations of how the growing calcite single crystals change their habit to satisfy the curvature of the organic phase.In this work,we examine calcite crystallization on a flat surface of glass slide and a curved surface of polystyrene（PS） sphere.The crystals exhibit tiny contact area onto the glass substrate that is averagely only 15%of their projected area on the substrate.In sharp contrast,the contact area greatly increase to above 75%of the projected area,once magnesium ions or agarose gel networks are introduced into the crystallization media.Furthermore,the calcite crystals form rough and step-like interfaces with a curved surface.However,the interfaces become smooth and curved as the crystals grow in presence of magnesium ions or agarose gel networks.The discrepancy between the interfacial structures implies kinetic effects of the additives on the crystallization around the surfaces.This work may provide implications for understanding the formation mechanisms of single-crystal composite materials.

All-perfluoropolymer,nonlinear stability-assisted monolithic surface combines topology-specific superwettability with ultradurability

Developing versatile and robust surfaces that mimic the skins of living beings to regulate air/liquid/solid matter is critical for many bioinspired applications.Despite notable achievements,such as in the case of developing robust superhydrophobic surfaces,it remains elusive to realize simultaneously topology-specific superwettability and multipronged durability owing to their inherent tradeoff and the lack of a scalable fabrication method.Here,we present a largely unexplored strategy of preparing an all-perfluoropolymer(Teflon),nonlinear stability-assisted monolithic surface for efficient regulating matters.