<strong>Aim:</strong> Diabetes Mellitus is a global public health challenge with major and potentially devastating complications, and concomitant complications include retinopathy and neuropathy due to hyp...<strong>Aim:</strong> Diabetes Mellitus is a global public health challenge with major and potentially devastating complications, and concomitant complications include retinopathy and neuropathy due to hypoxia and microvascular dysfunction. In this study, we investigated the effect of hyperbaric oxygen therapy as a method to transiently improve tissue oxygenation on diabetic retinopathy and neuropathy in a streptozotocin induced type-1 diabetic rat model (Wistar). <strong>Methods:</strong> Streptozotocin induced type-1 diabetic rats received 10 sessions of 2-h hyperbaric oxygen exposures (pO<sub>2</sub> = 309 kPa) over 2 weeks. Animals were exposed to light stimuli to produce light evoked potentials to estimate the effect of oxygen treatment on diabetic retinopathy. Sciatic nerves were exposed and stimulated to produce muscle evoked potential, which were recorded in the muscles of the foot and subsequently used to evaluate the effect of oxygen treatment on diabetic neuropathy. <strong>Results:</strong> We found significantly shorter light evoked potential latency and increased amplitude in hyperbaric oxygen treated animals. No change was found in nerve conduction. <strong>Conclusions:</strong> This study showed that hyperbaric oxygen therapy is a potentially effective treatment for diabetic retinopathy, improving both latency and amplitude of light evoked potentials.展开更多
Hyperpolarized(HP)^(13)C magnetic resonance imaging(MRI)is a powerful molecular imaging technique.With dissolution dynamic nuclear polarization(d-DNP),it can achieve dramatic signal increase and allows for dynamic and...Hyperpolarized(HP)^(13)C magnetic resonance imaging(MRI)is a powerful molecular imaging technique.With dissolution dynamic nuclear polarization(d-DNP),it can achieve dramatic signal increase and allows for dynamic and quantitative investigation of real-time metabolism in vivo.[1]The typical HP^(13)C MRI workflow begins with formulating the^(13)C probe,and then placing it in the hyperpolarization equipment to achieve the desired signal amplification level,which typically amounts to an increase in the MRI signal by more than 10,000 folds.The subsequent data acquisition is supported by a specialized^(13)C coil and^(13)C acquisition sequence,and finally quantitative and visualized metabolic information is obtained through a post-processing algorithm.To date,research of HP^(13)C MRI has focused on diabetes,cardiovascular diseases,and cancers,[2]showing great promises in tumor detection and the evaluation of therapeutic efficacy.展开更多
文摘<strong>Aim:</strong> Diabetes Mellitus is a global public health challenge with major and potentially devastating complications, and concomitant complications include retinopathy and neuropathy due to hypoxia and microvascular dysfunction. In this study, we investigated the effect of hyperbaric oxygen therapy as a method to transiently improve tissue oxygenation on diabetic retinopathy and neuropathy in a streptozotocin induced type-1 diabetic rat model (Wistar). <strong>Methods:</strong> Streptozotocin induced type-1 diabetic rats received 10 sessions of 2-h hyperbaric oxygen exposures (pO<sub>2</sub> = 309 kPa) over 2 weeks. Animals were exposed to light stimuli to produce light evoked potentials to estimate the effect of oxygen treatment on diabetic retinopathy. Sciatic nerves were exposed and stimulated to produce muscle evoked potential, which were recorded in the muscles of the foot and subsequently used to evaluate the effect of oxygen treatment on diabetic neuropathy. <strong>Results:</strong> We found significantly shorter light evoked potential latency and increased amplitude in hyperbaric oxygen treated animals. No change was found in nerve conduction. <strong>Conclusions:</strong> This study showed that hyperbaric oxygen therapy is a potentially effective treatment for diabetic retinopathy, improving both latency and amplitude of light evoked potentials.
基金National Natural Science Foundation of China(No.82302161)China Postdoctoral Science Foundation(No.2023M732464)
文摘Hyperpolarized(HP)^(13)C magnetic resonance imaging(MRI)is a powerful molecular imaging technique.With dissolution dynamic nuclear polarization(d-DNP),it can achieve dramatic signal increase and allows for dynamic and quantitative investigation of real-time metabolism in vivo.[1]The typical HP^(13)C MRI workflow begins with formulating the^(13)C probe,and then placing it in the hyperpolarization equipment to achieve the desired signal amplification level,which typically amounts to an increase in the MRI signal by more than 10,000 folds.The subsequent data acquisition is supported by a specialized^(13)C coil and^(13)C acquisition sequence,and finally quantitative and visualized metabolic information is obtained through a post-processing algorithm.To date,research of HP^(13)C MRI has focused on diabetes,cardiovascular diseases,and cancers,[2]showing great promises in tumor detection and the evaluation of therapeutic efficacy.
