The role of acrylamide on the human peripheral lymphocytes was studied by laser scanning confocal microscopy technique and fluo-3. The calibration value of the apparent dissociation constant （Kd） of the fluo-3-Ca^2...The role of acrylamide on the human peripheral lymphocytes was studied by laser scanning confocal microscopy technique and fluo-3. The calibration value of the apparent dissociation constant （Kd） of the fluo-3-Ca^2＋ complex was obtained as 4.83 × 10^-7 moi/L. Acrylamide （〈54 μg/mL） evoked a rise in free intracellular calcium concentration [Ca^2＋]i, in a dosedependent manner. Acrylamide induced the increase of [Ca^2＋]i was discussed in detail.展开更多
Characteristic of Fura-2-Ca^2＋ interaction was studied based on the fluorescence technique. The apparent dissociation constants （Kd） of the Fura-2-Ca^2＋ complex were determined at different temperature. The effect...Characteristic of Fura-2-Ca^2＋ interaction was studied based on the fluorescence technique. The apparent dissociation constants （Kd） of the Fura-2-Ca^2＋ complex were determined at different temperature. The effect of cefotaxime （CEFA） on intracellular Ca^2＋ concentration （[Ca^2＋]i） was discussed by using a ratiometric fluorescence dye Fura-2 as a probe. The basal [Ca^2＋]i in resting human peripheral lymphocytes was 100 4- 7 nmol/L but after treatment with cefotaxime, the changes of [Ca^2＋]i were observed in different conditions. In the concentration range of 1-30 μmol/L of cefotaxime [Ca^2＋]i increased, as a result of releasing intracellular Ca^2＋ stores. Higher concentration of cefotaxime （50-500 μmol/L） stimulated to decrease of [Ca^2＋]i.展开更多
The effect of penicillin on the human peripheral lymphocytes was studied by steady fluorescent technique and ratiometric fluorescence dye, Fura-2, The change of the free calcium concentration in cytosol was examined u...The effect of penicillin on the human peripheral lymphocytes was studied by steady fluorescent technique and ratiometric fluorescence dye, Fura-2, The change of the free calcium concentration in cytosol was examined under different conditions. A characterization of Fura-2-Ca interaction in an isotonic saline solution showed that Ca^2＋ formed a 1：1 Fura-2-Ca complex with the apparent dissociation constant 1.81×10^-7 mol/L. The mechanism, by which penicillin induced the decrease of [Ca^2＋]i , was discussed in detail. The low dose of penicillin might modify the lymphocytes＇ immunology response by interfering the increase in the intracellular free calcium concentration.展开更多
基金This work was supported by the National Natural Science Foundation of China （No. 20575038）
文摘The role of acrylamide on the human peripheral lymphocytes was studied by laser scanning confocal microscopy technique and fluo-3. The calibration value of the apparent dissociation constant （Kd） of the fluo-3-Ca^2＋ complex was obtained as 4.83 × 10^-7 moi/L. Acrylamide （〈54 μg/mL） evoked a rise in free intracellular calcium concentration [Ca^2＋]i, in a dosedependent manner. Acrylamide induced the increase of [Ca^2＋]i was discussed in detail.
基金the National Natural Science Foundation of China （No. 20575038）.
文摘Characteristic of Fura-2-Ca^2＋ interaction was studied based on the fluorescence technique. The apparent dissociation constants （Kd） of the Fura-2-Ca^2＋ complex were determined at different temperature. The effect of cefotaxime （CEFA） on intracellular Ca^2＋ concentration （[Ca^2＋]i） was discussed by using a ratiometric fluorescence dye Fura-2 as a probe. The basal [Ca^2＋]i in resting human peripheral lymphocytes was 100 4- 7 nmol/L but after treatment with cefotaxime, the changes of [Ca^2＋]i were observed in different conditions. In the concentration range of 1-30 μmol/L of cefotaxime [Ca^2＋]i increased, as a result of releasing intracellular Ca^2＋ stores. Higher concentration of cefotaxime （50-500 μmol/L） stimulated to decrease of [Ca^2＋]i.
基金This work was supported by the National Natural Science Foundation of China （No.20575038）the Foundation of Shanxi Provience （No.20031019）.
文摘The effect of penicillin on the human peripheral lymphocytes was studied by steady fluorescent technique and ratiometric fluorescence dye, Fura-2, The change of the free calcium concentration in cytosol was examined under different conditions. A characterization of Fura-2-Ca interaction in an isotonic saline solution showed that Ca^2＋ formed a 1：1 Fura-2-Ca complex with the apparent dissociation constant 1.81×10^-7 mol/L. The mechanism, by which penicillin induced the decrease of [Ca^2＋]i , was discussed in detail. The low dose of penicillin might modify the lymphocytes＇ immunology response by interfering the increase in the intracellular free calcium concentration.
