Serine protease 50(PRSS50/TSP50)is highly expressed in spermatocytes.Our study investigated its role in testicular development and spermatogenesis.Initially,PRSS50 knockdown was observed to impair DNA synthesis in spe...Serine protease 50(PRSS50/TSP50)is highly expressed in spermatocytes.Our study investigated its role in testicular development and spermatogenesis.Initially,PRSS50 knockdown was observed to impair DNA synthesis in spermatocytes.To further explore this,we generated PRSS50 knockout(Prss50^(−/−))mice(Mus musculus),which exhibited abnormal spermatid nuclear compression and reduced male fertility.Furthermore,dysplastic seminiferous tubules and decreased sex hormones were observed in 4-week-old Prss50^(−/−)mice,accompanied by meiotic progression defects and increased apoptosis of spermatogenic cells.Mechanistic analysis indicated that PRSS50 deletion resulted in increased phosphorylation of extracellular signal-regulated protein kinases 1 and 2(ERK1/2)and elevated levels of MAP kinase phosphatase 3(MKP3),a specific ERK antagonist,potentially accounting for testicular dysplasia in adolescent Prss50−/−mice.Taken together,these findings suggest that PRSS50 plays an important role in testicular development and spermatogenesis,with the MKP3/ERK signaling pathway playing a significant role in this process.展开更多
The dependence of Brillouin gain spectrum(BGS)characteristics,including the Brillouin frequency shift(BFS)and the BGS bandwidth,on germanium concentration in large-mode-area Ge-doped passive fibers is investigated the...The dependence of Brillouin gain spectrum(BGS)characteristics,including the Brillouin frequency shift(BFS)and the BGS bandwidth,on germanium concentration in large-mode-area Ge-doped passive fibers is investigated theoretically and experimentally.The simulation results show that the BFS is inversely proportional to GeO_(2)concentration,and the BGS bandwidth initially increases with the augment of GeO_(2)concentration,and then decreases.The BGSs of four fibers with core diameters of 10μm and 20μm for different GeO_(2)concentrations are compared experimentally.Experimental results demonstrate that with the same core diameter,the variations of BFS and BGS bandwidths with GeO_(2)concentration accord with the simulation results.Additionally,the BGS characteristics of three large-mode-area passive fibers with diameters of 10μm,25μm,and 30μm are measured,which confirm that the increasing of the fiber diameters will cause the BGS bandwidth to broaden.We believe that these results can provide valuable references for modulating the high-power narrowlinewidth fiber lasers and Brillouin fiber amplifiers.展开更多
基金supported by the Research Foundation of Jilin Provincial Science&Technology Development(20210204164YY,YDZJ202201ZYTS524,20230204067YY,20230204069YY)Jilin Province Development and Reform Commission(2022C044-3)Fundamental Research Funds for the Central Universities(135131002)。
文摘Serine protease 50(PRSS50/TSP50)is highly expressed in spermatocytes.Our study investigated its role in testicular development and spermatogenesis.Initially,PRSS50 knockdown was observed to impair DNA synthesis in spermatocytes.To further explore this,we generated PRSS50 knockout(Prss50^(−/−))mice(Mus musculus),which exhibited abnormal spermatid nuclear compression and reduced male fertility.Furthermore,dysplastic seminiferous tubules and decreased sex hormones were observed in 4-week-old Prss50^(−/−)mice,accompanied by meiotic progression defects and increased apoptosis of spermatogenic cells.Mechanistic analysis indicated that PRSS50 deletion resulted in increased phosphorylation of extracellular signal-regulated protein kinases 1 and 2(ERK1/2)and elevated levels of MAP kinase phosphatase 3(MKP3),a specific ERK antagonist,potentially accounting for testicular dysplasia in adolescent Prss50−/−mice.Taken together,these findings suggest that PRSS50 plays an important role in testicular development and spermatogenesis,with the MKP3/ERK signaling pathway playing a significant role in this process.
基金Project supported by the Key-Area Research and Development Program of Guangdong Province,China(Grant No.2018B090904001)the National Natural Science Foundation of China(Grant Nos.61805261,61405202,and 61705243)the Youth Innovation Promotion Association,Chinese Academy of Sciences(Grant No.2020252)。
文摘The dependence of Brillouin gain spectrum(BGS)characteristics,including the Brillouin frequency shift(BFS)and the BGS bandwidth,on germanium concentration in large-mode-area Ge-doped passive fibers is investigated theoretically and experimentally.The simulation results show that the BFS is inversely proportional to GeO_(2)concentration,and the BGS bandwidth initially increases with the augment of GeO_(2)concentration,and then decreases.The BGSs of four fibers with core diameters of 10μm and 20μm for different GeO_(2)concentrations are compared experimentally.Experimental results demonstrate that with the same core diameter,the variations of BFS and BGS bandwidths with GeO_(2)concentration accord with the simulation results.Additionally,the BGS characteristics of three large-mode-area passive fibers with diameters of 10μm,25μm,and 30μm are measured,which confirm that the increasing of the fiber diameters will cause the BGS bandwidth to broaden.We believe that these results can provide valuable references for modulating the high-power narrowlinewidth fiber lasers and Brillouin fiber amplifiers.
