BACKGROUND Non-invasive methods to diagnose non-alcoholic steatohepatitis(NASH),an inflammatory subtype of non-alcoholic fatty liver disease(NAFLD),are currently unavailable.AIM To develop an integrin αvβ3-targeted ...BACKGROUND Non-invasive methods to diagnose non-alcoholic steatohepatitis(NASH),an inflammatory subtype of non-alcoholic fatty liver disease(NAFLD),are currently unavailable.AIM To develop an integrin αvβ3-targeted molecular imaging modality to differentiate NASH.METHODS Integrinαvβ3 expression was assessed in Human LO2 hepatocytes Scultured with palmitic and oleic acids(FFA).Hepatic integrinαvβ3 expression was analyzed in rabbits fed a high-fat diet(HFD)and in rats fed a high-fat,high-carbohydrate diet(HFCD).After synthesis,cyclic arginine-glycine-aspartic acid peptide(cRGD)was labeled with gadolinium(Gd)and used as a contrast agent in magnetic resonance imaging(MRI)performed on mice fed with HFCD.RESULTS Integrin αvβ3 was markedly expressed on FFA-cultured hepatocytes,unlike the control hepatocytes.Hepatic integrin αvβ3 expression significantly increased in both HFD-fed rabbits and HFCD-fed rats as simple fatty liver(FL)progressed to steatohepatitis.The distribution of integrinαvβ3 in the liver of NASH cases largely overlapped with albumin-positive staining areas.In comparison to mice with simple FL,the relative liver MRI-T1 signal value at 60 minutes post-injection of Gd-labeled cRGD was significantly increased in mice with steatohepatitis(P<0.05),showing a positive correlation with the NAFLD activity score(r=0.945;P<0.01).Hepatic integrin αvβ3 expression was significantly upregulated during NASH development,with hepatocytes being the primary cells expressing integrin αvβ3.CONCLUSION After using Gd-labeled cRGD as a tracer,NASH was successfully distinguished by visualizing hepatic integrin αvβ3 expression with MRI.展开更多
In this paper, the Dirichlet problem of Stokes approximate of non-homogeneous incompressible Navier-Stokes equations is studied. It is shown that there exist global weak solutions as well as global and unique strong s...In this paper, the Dirichlet problem of Stokes approximate of non-homogeneous incompressible Navier-Stokes equations is studied. It is shown that there exist global weak solutions as well as global and unique strong solution for this problem, under the assumption that initial density po(x) is bounded away from 0 and other appropriate assumptions (see Theorem 1 and Theorem 2). The semi-Galerkin method is applied to construct the approximate solutions and a prior estimates are made to elaborate upon the compactness of the approximate solutions.展开更多
In this paper,we consider the following quasilinear diferential equation(p(u′))′+λf(t,u)=0subject to one of the two boundary conditions:u(0)=u′(1)=0,u′(0)=u(1)=0.After transforming them into a pro...In this paper,we consider the following quasilinear diferential equation(p(u′))′+λf(t,u)=0subject to one of the two boundary conditions:u(0)=u′(1)=0,u′(0)=u(1)=0.After transforming them into a problem of symmetrical solutions,the existence of three solutions of the problem is obtained by using a recent critical point theorem of Recceri.An example is given to demonstrate our main result.展开更多
The main purpose of this paper is to prove the well-posedness of the two-dimensional Boussinesq equations when the initial vorticity wo C L^1 (R^2) (or the finite Radon measure space). Using the stream function fo...The main purpose of this paper is to prove the well-posedness of the two-dimensional Boussinesq equations when the initial vorticity wo C L^1 (R^2) (or the finite Radon measure space). Using the stream function form of the equations and the Schauder fixed-point theorem to get the new proof of these results, we get that when the initial vorticity is smooth, there exists a unique classical solutions for the Cauchy problem of the two dimensional Boussinesq equations.展开更多
基金Supported by the National Natural Science Foundation of China,No.81670513and Young Scientists Fund of the National Natural Science Foundation of China,No.81900511。
文摘BACKGROUND Non-invasive methods to diagnose non-alcoholic steatohepatitis(NASH),an inflammatory subtype of non-alcoholic fatty liver disease(NAFLD),are currently unavailable.AIM To develop an integrin αvβ3-targeted molecular imaging modality to differentiate NASH.METHODS Integrinαvβ3 expression was assessed in Human LO2 hepatocytes Scultured with palmitic and oleic acids(FFA).Hepatic integrinαvβ3 expression was analyzed in rabbits fed a high-fat diet(HFD)and in rats fed a high-fat,high-carbohydrate diet(HFCD).After synthesis,cyclic arginine-glycine-aspartic acid peptide(cRGD)was labeled with gadolinium(Gd)and used as a contrast agent in magnetic resonance imaging(MRI)performed on mice fed with HFCD.RESULTS Integrin αvβ3 was markedly expressed on FFA-cultured hepatocytes,unlike the control hepatocytes.Hepatic integrin αvβ3 expression significantly increased in both HFD-fed rabbits and HFCD-fed rats as simple fatty liver(FL)progressed to steatohepatitis.The distribution of integrinαvβ3 in the liver of NASH cases largely overlapped with albumin-positive staining areas.In comparison to mice with simple FL,the relative liver MRI-T1 signal value at 60 minutes post-injection of Gd-labeled cRGD was significantly increased in mice with steatohepatitis(P<0.05),showing a positive correlation with the NAFLD activity score(r=0.945;P<0.01).Hepatic integrin αvβ3 expression was significantly upregulated during NASH development,with hepatocytes being the primary cells expressing integrin αvβ3.CONCLUSION After using Gd-labeled cRGD as a tracer,NASH was successfully distinguished by visualizing hepatic integrin αvβ3 expression with MRI.
基金the National Natural Science Foundation of China(No.10431060)
文摘In this paper, the Dirichlet problem of Stokes approximate of non-homogeneous incompressible Navier-Stokes equations is studied. It is shown that there exist global weak solutions as well as global and unique strong solution for this problem, under the assumption that initial density po(x) is bounded away from 0 and other appropriate assumptions (see Theorem 1 and Theorem 2). The semi-Galerkin method is applied to construct the approximate solutions and a prior estimates are made to elaborate upon the compactness of the approximate solutions.
基金Supported by the National Natural Science Foundation of Ministry of Education of Beijing(No.KM200810772010)Sponsored by the Science Research Foundation of Beijing Information Science and Tech-nology University(5026010948)
文摘In this paper,we consider the following quasilinear diferential equation(p(u′))′+λf(t,u)=0subject to one of the two boundary conditions:u(0)=u′(1)=0,u′(0)=u(1)=0.After transforming them into a problem of symmetrical solutions,the existence of three solutions of the problem is obtained by using a recent critical point theorem of Recceri.An example is given to demonstrate our main result.
基金supported by the National Natural Science Foundation of China (No. 11171229)supported by 973 program (Grant No. 2011CB711100)
文摘The main purpose of this paper is to prove the well-posedness of the two-dimensional Boussinesq equations when the initial vorticity wo C L^1 (R^2) (or the finite Radon measure space). Using the stream function form of the equations and the Schauder fixed-point theorem to get the new proof of these results, we get that when the initial vorticity is smooth, there exists a unique classical solutions for the Cauchy problem of the two dimensional Boussinesq equations.