金振口服液治疗呼吸道合胞病毒感染引起咳嗽的作用机制研究

目的探讨金振口服液治疗呼吸道合胞病毒感染幼年豚鼠咳嗽的作用及其机制。方法选取幼年豚鼠,随机分为空白组、模型组、磷酸苯丙哌林组、金振口服液(1.3、2.6g/kg)组。除空白组外,每鼠用10^(6.29) TCID_(50) mL^(-1)滴度的呼吸道合胞病毒(RSV)溶液滴鼻造模,空白组给予等体积无病毒DMEM溶液,次日起各给药组连续给药7d。第8天用枸橼酸引咳法检测豚鼠5min内咳嗽次数与咳嗽潜伏期,计数肺泡灌洗液中白细胞总数,ELISA法检测肺泡灌洗液中TNF?α、IL-4、IL-13含量,HE染色进行肺组织学检查,免疫组织化学染色法检测支气管中神经生长因子(NGF)、P物质(SP)、神经肽受体1(NK-1)的表达,Western blot法检测肺组织中NGF、NK-1的表达。结果金振口服液能够抑制RSV感染导致的豚鼠咳嗽次数增多,下调肺泡灌洗液中白细胞总数及TNF?α、IL-4、IL-13的含量,改善肺组织的炎性浸润,降低支气管NGF、SP和NK-1及肺组织NGF和SP的表达。结论金振口服液能改善RSV感染引起的病毒性咳嗽,其机制可能与减少气道炎性反应,降低神经源性介质,调节咳嗽敏感性有关。

动态浊度法定量检测注射用氟氧头孢钠中细菌内毒素的含量

目的:采用动态浊度法检测注射用氟氧头孢钠中细菌内毒素的含量。方法:通过建立细菌内毒素标准曲线,干扰预试验确定供试品的无干扰浓度,确定动态浊度检测方法。同时,验证方法的精密性和准确性,并对供试品中细菌内毒素的含量进行定量测定。结果:标准曲线的线性范围为0.01~1.25 EU/ml,相关系数(r)> 0.980,标准曲线成立;供试品终浓度在0.4 mg/ml时回收率最接近100%,对内毒素测定的干扰较小。供试品中加入不同浓度标准内毒素的回收率为84%~120%,3次内毒素标准曲线测量结果的变异系数均<10%,方法的准确性和精密度较好。用该方法检测供试品的细菌内毒素含量均小于检测限。结论:动态浊度法能够用于注射用氟氧头孢钠中细菌内毒素的定量检测。

注射用头孢哌酮钠舒巴坦钠的凝血功能评价研究

目的 探讨注射用头孢哌酮钠舒巴坦钠对凝血酶原时间(PT)、活化部分凝血酶时间(APTT)的影响。方法 选取8个不同原料药厂家的8批样品,将家兔随机分为8个给药组,并另设肝素钠注射液(1869单位/kg)作为阳性对照组,生理盐水作为阴性对照组,静脉给药5天后,取家兔血浆,用试剂盒检测PT及APTT的值。结果 各原料药厂的注射用头孢哌酮钠舒巴坦钠对PT的影响较小,较正常值增幅均小于3 s,(P>0.05);而各原料药厂的注射用头孢哌酮钠舒巴坦钠均能延长APTT的时间(P<0.05)。结论 注射用头孢哌酮钠舒巴坦钠可能会延长APTT,对凝血功能产生影响。

Food-advanced glycation end products aggravate the diabetic vascular complications via modulating the AGEs/RAGE pathway

The aim of this study was to investigate the effects of high-advanced glycation end products （AGEs） diet on diabetic vascular complications. The Streptozocin （STZ）-induced diabetic mice were fed with high-AGEs diet. Diabetic characteristics, indicators 3f renal and cardiovascular functions, and pathohistology of pancreas, heart and renal were evaluated. AGEs/RAGE/ROS pathway parameters were determined. During the experiments, the diabetic mice exhibited typical characteristics including weight loss, polydipsia, polyphagia, polyuria, high-blood glucose, and low-serum insulin levels. However, high-AGEs diet effectively aggravated these diabetic sharacteristics. It also increased the 24-h urine protein levels, serum levels of urea nitrogen, creatinine, c-reactive protein （CRP）, low density lipoprotein （LDL）, tumor necrosis factor-a （TNF-a）, and interleukin-6 （IL-6） in the diabetic mice. High-AGEs diet deteriorated the histology of pancreas, heart, and kidneys, and caused structural alterations of endothelial ceils, mesangial cells and podocytes in renal ：ortex. Eventually, high-AGEs diet contributed to the high-AGE levels in serum and kidneys, high-levels of reactive oxygen species ＇,ROS） and low-levels of superoxide dismutase （SOD） in serum, heart, and kidneys. It also upregulated RAGE mRNA and protein expression in heart and kidneys. Our results showed that high-AGEs diet deteriorated vascular complications in the diabetic mice. The activation of AGEs/RAGE/ROS pathway may be involved in the pathogenesis of vascular complications in diabetes.

722件进口精蛋白锌重组赖脯胰岛素混合注射液质量研究

目的:对进口精蛋白锌重组赖脯胰岛素混合注射液进行质量分析。方法:对2018~2020年经苏州口岸进口的129批(722件)精蛋白锌重组赖脯胰岛素混合注射液的有关物质、苯酚和间甲酚、可溶性赖脯胰岛素、含量、锌、高分子蛋白质、pH等质量参数进行统计分析,并对结果进行趋势分析。结果:精蛋白锌重组赖脯胰岛素混合注射液(25R)36批352件,占48.8%,精蛋白锌重组赖脯胰岛素混合注射液(50R)93批370件,占51.2%。722件精蛋白锌重组赖脯胰岛素混合注射液关键参数检测结果主要位于平均值±2SD区间。苯酚和间甲酚、可溶性赖脯胰岛素、含量、锌、pH等均未超过平均值±3SD,但部分批次产品的有关物质和高分子蛋白质超过平均值±3SD。结论:精蛋白锌重组赖脯胰岛素混合注射液总体情况稳定,质量较好。