Rice direct seeding has the significant potential to save labor and water,conserve environmental resources,and reduce greenhouse gas emissions tremendously.Therefore,rice direct seeding is becoming the major cultivati...Rice direct seeding has the significant potential to save labor and water,conserve environmental resources,and reduce greenhouse gas emissions tremendously.Therefore,rice direct seeding is becoming the major cultivation technology applied to rice production in many countries.Identifying and utilizing genes controlling mesocotyl elongation is an effective approach to accelerate breeding procedures and meet the requirements for direct-seeded rice(DSR) production.This study used a permanent mapping population with 144 recombinant inbred lines(RILs) and 2 828 bin-markers to detect quantitative trait loci(QTLs) associated with mesocotyl length in 2019 and 2020.The mesocotyl lengths of the rice RILs and their parents,Lijiangxintuanheigu(LTH) and Shennong 265(SN265),were measured in a growth chamber at 30°C in a dark environment.A total of 16 QTLs for mesocotyl length were identified on chromosomes 1(2),2(4),3(2),4,5,6,7,9,11(2),and 12.Seven of these QTLs,including qML1a,qML1b,qML2d,qML3a,qML3b,qML5,and qML11b,were reproducibly detected in both years via the interval mapping method.The major QTL,qML3a,was reidentified in two years via the composite interval mapping method.A total of 10 to 413 annotated genes for each QTL were identified in their smallest genetic intervals of 37.69 kb to 2.78 Mb,respectively.Thirteen predicted genes within a relatively small genetic interval(88.18 kb) of the major mesocotyl elongation QTL,qML3a,were more thoroughly analyzed.Finally,the coding DNA sequence variations among SN265,LTH,and Nipponbare indicated that the LOC_Os03g50550 gene was the strongest candidate gene for the qML3a QTL controlling the mesocotyl elongation.This LOC_Os03g50550 gene encodes a mitogen-activated protein kinase.Relative gene expression analysis using qRT-RCR further revealed that the expression levels of the LOC_Os03g50550 gene in the mesocotyl of LTH were significantly lower than in the mesocotyl of SN265.In conclusion,these results further strengthen our knowledge about rice’s genetic mechanisms of mesocotyl elongation.This investigation’s discoveries will help to accelerate breeding programs for new DSR variety development.展开更多
The ligand ethylenediamine-3,5-dichloro salicylaldehyde（HS） and its crystal Co（Ⅱ） complex（MS） were yielded.The compounds have been characterized on the basis of elemental analysis,IR and 1H NMR spectral studies...The ligand ethylenediamine-3,5-dichloro salicylaldehyde（HS） and its crystal Co（Ⅱ） complex（MS） were yielded.The compounds have been characterized on the basis of elemental analysis,IR and 1H NMR spectral studies.X-ray single-crystal diffraction of crystal MS1 showed a classical Salen-N2O2 tetrahedral coordination model,space group P1 with a = 25.73（2）,b = 9.899（9）,c = 6.753（6） A,β = 103.467（10）o,V = 1673（3） A^3,Z = 4,C16H10Cl4 Co N2O2,Mr = 462.99,Dc = 1.838 g/cm^3,F（000） = 924,μ（Mo Kα） = 1.678 mm^-1（λ = 0.71073 A）,R = 0.0275 and w R = 0.0567 for 1247 observed reflections with I 〉 2σ（I）.Furthermore,luminescent properties were evaluated by UV-Visible absorption and FL spectra.Meanwhile,the thermal stability of compound HS was investigated.TG-DTG showed that the decomposition temperature（Td） of the molecules is up to 256 ℃ and the compound has fine performance in thermal stability.The results also indicated that the target compound HS exhibited certain chemiluminescence properties under the conditions of different time of 365 nm UV light and different temperature.In general,this kind of multi-functional compounds has potential applications in the fields of information storage and nonlinear optical materials.展开更多
基金supported by grants from the Natural Science Foundation of Heilongjiang Province, China (LH2020C098)the Fundamental Research Funds for the Research Institutes of Heilongjiang Province, China (CZKYF2020A001)+1 种基金the National Key Research and Development Program of China (2016YFD0300104)the Heilongjiang Province Agricultural Science and Technology Innovation Project, China (2020JCQN001, 2019JJPY007, 2020FJZX049, 2021QKPY009, 2021CQJC003)。
文摘Rice direct seeding has the significant potential to save labor and water,conserve environmental resources,and reduce greenhouse gas emissions tremendously.Therefore,rice direct seeding is becoming the major cultivation technology applied to rice production in many countries.Identifying and utilizing genes controlling mesocotyl elongation is an effective approach to accelerate breeding procedures and meet the requirements for direct-seeded rice(DSR) production.This study used a permanent mapping population with 144 recombinant inbred lines(RILs) and 2 828 bin-markers to detect quantitative trait loci(QTLs) associated with mesocotyl length in 2019 and 2020.The mesocotyl lengths of the rice RILs and their parents,Lijiangxintuanheigu(LTH) and Shennong 265(SN265),were measured in a growth chamber at 30°C in a dark environment.A total of 16 QTLs for mesocotyl length were identified on chromosomes 1(2),2(4),3(2),4,5,6,7,9,11(2),and 12.Seven of these QTLs,including qML1a,qML1b,qML2d,qML3a,qML3b,qML5,and qML11b,were reproducibly detected in both years via the interval mapping method.The major QTL,qML3a,was reidentified in two years via the composite interval mapping method.A total of 10 to 413 annotated genes for each QTL were identified in their smallest genetic intervals of 37.69 kb to 2.78 Mb,respectively.Thirteen predicted genes within a relatively small genetic interval(88.18 kb) of the major mesocotyl elongation QTL,qML3a,were more thoroughly analyzed.Finally,the coding DNA sequence variations among SN265,LTH,and Nipponbare indicated that the LOC_Os03g50550 gene was the strongest candidate gene for the qML3a QTL controlling the mesocotyl elongation.This LOC_Os03g50550 gene encodes a mitogen-activated protein kinase.Relative gene expression analysis using qRT-RCR further revealed that the expression levels of the LOC_Os03g50550 gene in the mesocotyl of LTH were significantly lower than in the mesocotyl of SN265.In conclusion,these results further strengthen our knowledge about rice’s genetic mechanisms of mesocotyl elongation.This investigation’s discoveries will help to accelerate breeding programs for new DSR variety development.
基金Supported by the NNSFC(No.2116606)Guangxi Postgraduate Innovative Research Project(No.YCSZ2014155)
文摘The ligand ethylenediamine-3,5-dichloro salicylaldehyde（HS） and its crystal Co（Ⅱ） complex（MS） were yielded.The compounds have been characterized on the basis of elemental analysis,IR and 1H NMR spectral studies.X-ray single-crystal diffraction of crystal MS1 showed a classical Salen-N2O2 tetrahedral coordination model,space group P1 with a = 25.73（2）,b = 9.899（9）,c = 6.753（6） A,β = 103.467（10）o,V = 1673（3） A^3,Z = 4,C16H10Cl4 Co N2O2,Mr = 462.99,Dc = 1.838 g/cm^3,F（000） = 924,μ（Mo Kα） = 1.678 mm^-1（λ = 0.71073 A）,R = 0.0275 and w R = 0.0567 for 1247 observed reflections with I 〉 2σ（I）.Furthermore,luminescent properties were evaluated by UV-Visible absorption and FL spectra.Meanwhile,the thermal stability of compound HS was investigated.TG-DTG showed that the decomposition temperature（Td） of the molecules is up to 256 ℃ and the compound has fine performance in thermal stability.The results also indicated that the target compound HS exhibited certain chemiluminescence properties under the conditions of different time of 365 nm UV light and different temperature.In general,this kind of multi-functional compounds has potential applications in the fields of information storage and nonlinear optical materials.
