The UASB reactor was used to reconstruct leachate treatment project of Beijing Asuwei Waste Sanitary Landfill Site,and the commissioning with the UASB reactor was executed.Water quality indicators were determined in t...The UASB reactor was used to reconstruct leachate treatment project of Beijing Asuwei Waste Sanitary Landfill Site,and the commissioning with the UASB reactor was executed.Water quality indicators were determined in the debugging process,and the results showed that the VFA content in the anaerobic tank was controlled within 600 mg/L,which indicated that the water quality did not have the acidified phenomenon.The COD removal efficiency was 50%approximately and NH_3-N concentration showed as light decline when operation stability in anaerobic system.展开更多
Tetraploid fish are important for mass production of triploids in polyploid breeding.In this study,we reported a novel protocol for artificial induction of tetraploidy by a combination of cold shock and hydrostatic pr...Tetraploid fish are important for mass production of triploids in polyploid breeding.In this study,we reported a novel protocol for artificial induction of tetraploidy by a combination of cold shock and hydrostatic pressure administered to gynogenetically developed eggs in Japanese flounder Paralichthys olivaceus.The induction was carried out by activating the eggs with UV-irradiated sperm of red sea bream Pagrus major,administering a cold shock(0℃,5 to 45 min)3 min after fertilization to inhibit second polar body exclusion,incubating the eggs for 60 min at 17℃,and treating them with a 650 kg/cm2 hydrostatics pressure shock for 6 min.We named the embryos gyno-tetraploids that developed from eggs after such treatments.The hatching rate of the gyno-tetraploids ranged from 20.99%±3.66%to 36.01%±2.79%,and the tetraploid rate ranged from 80.00%to 100.00%.All-maternal inheritance was verified using 6 high-recombination-rate microsatellite markers.This method successfully induced gyno-tetraploidy.The successful induction of gyno-tetraploidy lays the foundation for triploidization of new varieties with improved economic traits of interest that can benefit commercial culture.展开更多
文摘The UASB reactor was used to reconstruct leachate treatment project of Beijing Asuwei Waste Sanitary Landfill Site,and the commissioning with the UASB reactor was executed.Water quality indicators were determined in the debugging process,and the results showed that the VFA content in the anaerobic tank was controlled within 600 mg/L,which indicated that the water quality did not have the acidified phenomenon.The COD removal efficiency was 50%approximately and NH_3-N concentration showed as light decline when operation stability in anaerobic system.
基金Supported by the Central Public-Interest Scientific Institution Basal Research Fund,CAFS(No.2016HY-ZD0202)the China Agriculture Research System(No.CARS-47)the Applied National Science and Technology Support Program in China(No.2012BAD26B01)
文摘Tetraploid fish are important for mass production of triploids in polyploid breeding.In this study,we reported a novel protocol for artificial induction of tetraploidy by a combination of cold shock and hydrostatic pressure administered to gynogenetically developed eggs in Japanese flounder Paralichthys olivaceus.The induction was carried out by activating the eggs with UV-irradiated sperm of red sea bream Pagrus major,administering a cold shock(0℃,5 to 45 min)3 min after fertilization to inhibit second polar body exclusion,incubating the eggs for 60 min at 17℃,and treating them with a 650 kg/cm2 hydrostatics pressure shock for 6 min.We named the embryos gyno-tetraploids that developed from eggs after such treatments.The hatching rate of the gyno-tetraploids ranged from 20.99%±3.66%to 36.01%±2.79%,and the tetraploid rate ranged from 80.00%to 100.00%.All-maternal inheritance was verified using 6 high-recombination-rate microsatellite markers.This method successfully induced gyno-tetraploidy.The successful induction of gyno-tetraploidy lays the foundation for triploidization of new varieties with improved economic traits of interest that can benefit commercial culture.
