Objective The study aimed to estimate the benchmark dose(BMD)of coke oven emissions(COEs)exposure based on mitochondrial damage with the mitochondrial DNA copy number(mtDNAcn)as a biomarker.Methods A total of 782 subj...Objective The study aimed to estimate the benchmark dose(BMD)of coke oven emissions(COEs)exposure based on mitochondrial damage with the mitochondrial DNA copy number(mtDNAcn)as a biomarker.Methods A total of 782 subjects were recruited,including 238 controls and 544 exposed workers.The mtDNAcn of peripheral leukocytes was detected through the real-time fluorescence-based quantitative polymerase chain reaction.Three BMD approaches were used to calculate the BMD of COEs exposure based on the mitochondrial damage and its 95%confidence lower limit(BMDL).Results The mtDNAcn of the exposure group was lower than that of the control group(0.60±0.29 vs.1.03±0.31;P<0.001).A dose-response relationship was shown between the mtDNAcn damage and COEs.Using the Benchmark Dose Software,the occupational exposure limits(OELs)for COEs exposure in males was 0.00190 mg/m^(3).The OELs for COEs exposure using the BBMD were 0.00170 mg/m^(3)for the total population,0.00158 mg/m^(3)for males,and 0.00174 mg/m^(3)for females.In possible risk obtained from animal studies(PROAST),the OELs of the total population,males,and females were 0.00184,0.00178,and 0.00192 mg/m^(3),respectively.Conclusion Based on our conservative estimate,the BMDL of mitochondrial damage caused by COEs is0.002 mg/m^(3).This value will provide a benchmark for determining possible OELs.展开更多
Organophosphorus pesticides(OPs)are extensively used for their high efficiency,broad spectrum,and low residue.However,the health hazards caused by long-term,low-dose exposure to OPs are easily ignored.Omethoate is a l...Organophosphorus pesticides(OPs)are extensively used for their high efficiency,broad spectrum,and low residue.However,the health hazards caused by long-term,low-dose exposure to OPs are easily ignored.Omethoate is a large class of OPs that is widely used in China.The inhibition of the cholinesterase(ChE)activity is the main toxicity mechanism of OPs,and such an activity is used as a biomarker of exposure to OPs.展开更多
Omethoate is a highly toxic organophosphorus pesticide that is widely used in agricultural production because of its high efficiency,broad spectrum,and low residue.Organophos phorus pesticides,such as omethoate,can in...Omethoate is a highly toxic organophosphorus pesticide that is widely used in agricultural production because of its high efficiency,broad spectrum,and low residue.Organophos phorus pesticides,such as omethoate,can inhibit acetylcholinesterase activity,leading to the accumulation of the neurotransmitter acetylcholine at cholinergic synapses.展开更多
基金supported by the National Natural Science Foundation of China[grant numbers:NSFC81872597,81001239]。
文摘Objective The study aimed to estimate the benchmark dose(BMD)of coke oven emissions(COEs)exposure based on mitochondrial damage with the mitochondrial DNA copy number(mtDNAcn)as a biomarker.Methods A total of 782 subjects were recruited,including 238 controls and 544 exposed workers.The mtDNAcn of peripheral leukocytes was detected through the real-time fluorescence-based quantitative polymerase chain reaction.Three BMD approaches were used to calculate the BMD of COEs exposure based on the mitochondrial damage and its 95%confidence lower limit(BMDL).Results The mtDNAcn of the exposure group was lower than that of the control group(0.60±0.29 vs.1.03±0.31;P<0.001).A dose-response relationship was shown between the mtDNAcn damage and COEs.Using the Benchmark Dose Software,the occupational exposure limits(OELs)for COEs exposure in males was 0.00190 mg/m^(3).The OELs for COEs exposure using the BBMD were 0.00170 mg/m^(3)for the total population,0.00158 mg/m^(3)for males,and 0.00174 mg/m^(3)for females.In possible risk obtained from animal studies(PROAST),the OELs of the total population,males,and females were 0.00184,0.00178,and 0.00192 mg/m^(3),respectively.Conclusion Based on our conservative estimate,the BMDL of mitochondrial damage caused by COEs is0.002 mg/m^(3).This value will provide a benchmark for determining possible OELs.
基金supported by Programs for Science and Technology Development of Zhengzhou[131PPTGG376]the Outstanding Youth Grant of Zhengzhou University[1521329035]
文摘Organophosphorus pesticides(OPs)are extensively used for their high efficiency,broad spectrum,and low residue.However,the health hazards caused by long-term,low-dose exposure to OPs are easily ignored.Omethoate is a large class of OPs that is widely used in China.The inhibition of the cholinesterase(ChE)activity is the main toxicity mechanism of OPs,and such an activity is used as a biomarker of exposure to OPs.
基金the Programs for Science and Technology Development of Zhengzhou[131PPTGG376]the Outstanding Youth Grant of Zhengzhou University[1521329035]。
文摘Omethoate is a highly toxic organophosphorus pesticide that is widely used in agricultural production because of its high efficiency,broad spectrum,and low residue.Organophos phorus pesticides,such as omethoate,can inhibit acetylcholinesterase activity,leading to the accumulation of the neurotransmitter acetylcholine at cholinergic synapses.
