Glutamatergic CYLD deletion leads to aberrant excitatory activity in the basolateral amygdala:association with enhanced cued fear expression

Neuronal activity,synaptic transmission,and molecular changes in the basolateral amygdala play critical roles in fear memory.Cylindromatosis(CYLD)is a deubiquitinase that negatively regulates the nuclear factor kappa-B pathway.CYLD is well studied in non-neuronal cells,yet underinvestigated in the brain,where it is highly expressed.Emerging studies have shown involvement of CYLD in the remodeling of glutamatergic synapses,neuroinflammation,fear memory,and anxiety-and autism-like behaviors.However,the precise role of CYLD in glutamatergic neurons is largely unknown.Here,we first proposed involvement of CYLD in cued fear expression.We next constructed transgenic model mice with specific deletion of Cyld from glutamatergic neurons.Our results show that glutamatergic CYLD deficiency exaggerated the expression of cued fear in only male mice.Further,loss of CYLD in glutamatergic neurons resulted in enhanced neuronal activation,impaired excitatory synaptic transmission,and altered levels of glutamate receptors accompanied by over-activation of microglia in the basolateral amygdala of male mice.Altogether,our study suggests a critical role of glutamatergic CYLD in maintaining normal neuronal,synaptic,and microglial activation.This may contribute,at least in part,to cued fear expression.

Network Curriculum Design of Traditional Chinese Medicine Pharmacy Specialty Formed by Introducing Guangxi Minority Medicine

Starting from the background and significance of the development and design of the network course of traditional Chinese medicine pharmacy specialty formed by introducing Guangxi minority medicine,this paper discusses the teaching design scheme and module construction of the network course in the development process,and summarizes the design,production and construction experience of the network course.Through practice,the teaching effect and achievement of the network course of traditional Chinese medicine pharmacy specialty formed by introducing Guangxi minority medicine are obvious.

Research and Practice on Curriculum Reform of Chinese Pharmaceutical Manufacturing Major against the Backdrop of"Comprehensive Health"

Against the backdrop of Comprehensive Health,the Chinese Pharmaceutical Manufacturing major faces both opportunities and challenges.This paper describes some problems encountered in the development of the Chinese Pharmaceutical Manufacturing major,puts forward some suggestions and measures to adapt to the training of talents of Chinese pharmaceutical manufacturing against the backdrop of Comprehensive Health,and discusses the practical research on curriculum reform.

Study on the influence of skid resistance on traffic safety of highway with a high ratio of bridges and tunnels

The safety of highways with a high ratio of bridges and tunnels is related to multiple factors,for example,the skid resistance of the pavement surface.In this study,the distribution of accidents under different conditions was calculated to investigate the relationship between the road skid resistance and the incidence of traffic accidents based on the traffic accident data of the Yuxiang highway.Statistical results show that weather conditions and road alignment may affect traffic accidents.The correlation analysis method was used to study the relationship between three factors and traffic accidents.The results show that road alignment,weather conditions and road skid resistance are related to the incidence of traffic accidents.The traffic accident prediction models were established based on back propagation neural network to verify the correlation analysis results.It is confirmed that road alignment,weather conditions and road skid resistance are the factors that affect traffic accidents.

Research progress on supramolecular structures of asphalt

The properties of asphalt binder play an essential role in the design life of asphalt pavement.Analysis of asphalt mechanical behaviors based on the chemical structures provides the theoretical basis for the improvement of asphalt performance in service life.Intermolecular interactions have a significant impact on the macroscopic properties of asphalt,a colloidal material,resulting in the parameters characterizing asphalt molecular structures fail to directly connect with mechanical properties of asphalt.This paper introduces the supramolecular theory,presents a brief review of existing supramolecular models and research methods of asphalt,aiming to explore a new methodology for the investigation between asphalt microstructures and macroscopic properties.Results showed the supramolecular theory focuses on the intermolecular interactions,providing more accurate indicators for the investigation on the asphalt supramolecules.Supramolecular analysis of asphalt has proposed feasible methods and established favorable connections with asphalt properties,giving a new view of understanding asphalt microstructure and a new approach to capture mechanical behaviors of asphalt.