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Elucidating the Uptake and Distribution of Nanoparticles in Solid Tumors via a Multilayered Cell Culture Model 被引量:4
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作者 darren yohan Charmainne Cruje +1 位作者 Xiaofeng Lu Devika Chithrani 《Nano-Micro Letters》 SCIE EI CAS 2015年第2期127-137,共11页
Multicellular layers(MCLs) have previously been used to determine the pharmacokinetics of a variety of different cancer drugs including paclitaxel, doxorubicin, methotrexate, and 5-fluorouracil across a number of cell... Multicellular layers(MCLs) have previously been used to determine the pharmacokinetics of a variety of different cancer drugs including paclitaxel, doxorubicin, methotrexate, and 5-fluorouracil across a number of cell lines. It is not known how nanoparticles(NPs) navigate through the tumor microenvironment once they leave the tumor blood vessel.In this study, we used the MCL model to study the uptake and penetration dynamics of NPs. Gold nanoparticles(GNPs)were used as a model system to map the NP distribution within tissue-like structures. Our results show that NP uptake and transport are dependent on the tumor cell type. MDA-MB-231 tissue showed deeper penetration of GNPs as compared to MCF-7 one. Intracellular and extracellular distributions of NPs were mapped using Cyto Viva imaging. The ability of MCLs to mimic tumor tissue characteristics makes them a useful tool in assessing the efficacy of particle distribution in solid tumors. 展开更多
关键词 Gold nanoparticles TUMOR Multicellular cell layers TISSUE
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Size-Dependent Gold Nanoparticle Interaction at Nano–Micro Interface Using Both Monolayer and Multilayer(Tissue-Like) Cell Models 被引量:2
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作者 darren yohan Charmainne Cruje +1 位作者 Xiaofeng Lu Devika B.Chithrani 《Nano-Micro Letters》 SCIE EI CAS 2016年第1期44-53,共10页
Gold nanoparticles(GNPs) are emerging as a novel tool to improve existing cancer therapeutics. GNPs are being used as radiation dose enhancers in radiation therapy as well as anticancer drugs carriers in chemotherapy.... Gold nanoparticles(GNPs) are emerging as a novel tool to improve existing cancer therapeutics. GNPs are being used as radiation dose enhancers in radiation therapy as well as anticancer drugs carriers in chemotherapy. However,the success of GNP-based therapeutics depends on their ability to penetrate tumor tissue. GNPs of 20 and 50 nm diameters were used to elucidate the effects of size on the GNP interaction with tumor cells at monolayer and multilayer level. At monolayer cell level, smaller NPs had a lower uptake compared to larger NPs at monolayer cell level. However, the order was reversed at tissue-like multilayer level. The smaller NPs penetrated better compared to larger NPs in tissue-like materials.Based on our study using tissue-like materials, we can predict that the smaller NPs are better for future therapeutics due to their greater penetration in tumor tissue once leaving the leaky blood vessels. In this study, tissue-like multilayer cellular structures(MLCs) were grown to model the post-vascular tumor environment. The MLCs exhibited a much more extensive extracellular matrix than monolayer cell cultures. The MLC model can be used to optimize the nano–micro interface at tissue level before moving into animal models. This would accelerate the use of NPs in future cancer therapeutics. 展开更多
关键词 Gold nanoparticles MULTILAYER cellular structures TISSUE PENETRATION HYPER spectral imaging Size dependence
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Optimization of Image Quality in Retrospective Respiratory-Gated Micro-CT for Quantitative Measurements of Lung Function in Free-Breathing Rats
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作者 Nancy L. Ford Andrew Jeklin +3 位作者 Karen Yip darren yohan David W. Holdsworth Maria Drangova 《Journal of Biomedical Science and Engineering》 2014年第4期157-172,共16页
Objective: To optimize scan time and X-ray dose with no loss of image quality for retrospectively gated micro-CT scans of free-breathing rats. Methods: Five free-breathing rats were scanned using a dynamic micro-CT sc... Objective: To optimize scan time and X-ray dose with no loss of image quality for retrospectively gated micro-CT scans of free-breathing rats. Methods: Five free-breathing rats were scanned using a dynamic micro-CT scanner over 10 continuous gantry rotations (50 seconds and entrance dose of 0.28 Gy). The in-phase projection views were selected and reconstructed, representing peak inspiration and end expiration from all 10 rotations and progressively fewer rotations. A least error method was also used to ensure that all angular positions were filled. Image quality and reproducibility for physiological measurements were compared for the two techniques. Results: The least error approach underestimated the lung volume, air content in the lung at peak inspiration, and tidal volume. Other measurements showed no differences between the projection-sorting techniques. Conclusions: Seven gantry rotations (35 seconds and 0.2 Gy dose) proved to be the optimal protocol for both the in-phase images and the least error images. 展开更多
关键词 Respiratory-Gating MICRO-CT Lung Imaging FREE-BREATHING RATS TIDAL Volume
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