Thermal-induced Unfolding of β-Crystallin and Disassembly of its Oligomers Revealed by Temperature-Jump Time-Resolved Infrared Spectroscopy

β-Crystallins are the major structural proteins existing in the vertebrate lens, and their conformational stability is critical in maintaining the life-long transparency and refraction index of the lens. Seven subunits of β-crystallins naturally assemble into various heteroge- neous oligomers with different sizes. Here, we systematically investigated the thermal sta- bility of the different secondary structures present in β-Crystallins and then the dynamic process for the thermal-induced unfolding of β-crystallins by Fourier transform infrared spectroscopy-monitored thermal titration and temperature-jump nanosecond time-resolved IR difference absorbance spectra. Our results show that the N-terminal anti-parallel β-sheets in β-crystallin are the most unstable with a transition midpoint temperature at 36.0-2.1℃, leading to the formation of an intermediate consisting vastly of random coil structures. This intermediate structure is temporally assigned to that of the monomer generated by the thermal-induced disassembly of β-crystallin oligomers with a transition midpoint tempera- ture of 40.4-0.7℃. The global unfolding of β-crystallins that leads to denaturation and aggregation indicated by the formation of intermolecular anti-parallel β-sheets has a transi- tion midpoint temperature determined as 72.4-0.2 ℃. Temperature-jump time-resolved IR absorbance difference spectroscopy analysis further reveals that thermal-induced unfolding of β-crystallins occurs firstly in the anti-parallel β-sheets in the N-terminal domains with a time constant of 50 ns.

Sweet spot prediction in tight sandstone reservoir based on well-bore rock physical simulation

To establish the relationship among reservoir characteristics and rock physical parameters,we construct the well-bore rock physical models firstly,considering the influence factors,such as mineral composition,shale content,porosity,fluid type and saturation.Then with analyzing the change rules of elastic parameters along with the above influence factors and the cross-plots among elastic parameters,the sensitive elastic parameters of tight sandstone reservoir are determined,and the rock physics template of sweet spot is constructed to guide pre-stack seismic inversion.The results show that velocity ratio and Poisson impedance are the most sensitive elastic parameters to indicate the lithologic and gas-bearing properties of sweet spot in tight sandstone reservoir.The high-quality sweet spot is characterized by the lower velocity ratio and Poisson impedance.Finally,the actual seismic data are selected to predict the sweet spots in tight sandstone gas reservoirs,so as to verify the validity of the rock physical simulation results.The significant consistency between the relative logging curves and inversion results in different wells implies that the utilization of well-bore rock physical simulation can guide the prediction of sweet spot in tight sandstone gas reservoirs.