Knockdown of the atypical protein kinase genes GhABC1K2-A05 and GhABC1K12-A07 make cotton more sensitive to salt and PEG stress

Activity of bc1 complex kinase(ABC1K)is an atypical protein kinase(aPK)that plays a crucial role in plant mitochondrial and plastid stress responses,but little is known about the responses of ABC1Ks to stress in cotton(Gossypium spp.).Here,we identified 40 ABC1Ks in upland cotton(Gossypium hirsutum L.)and found that the Gh ABC1Ks were unevenly distributed across 17 chromosomes.The GhABC1K family members included 35 paralogous gene pairs and were expanded by segmental duplication.The GhABC1K promoter sequences contained diverse cis-acting regulatory elements relevant to hormone or stress responses.The qRT-PCR results revealed that most Gh ABC1Ks were upregulated by exposure to different stresses.Gh ABC1K2-A05 and Gh ABC1K12-A07 expression levels were upregulated by at least three stress treatments.These genes were further functionally characterized by virus-induced gene silencing(VIGS).Compared with the controls,the Gh ABC1K2-A05-and Gh ABC1K12-A07-silenced cotton lines exhibited higher malondialdehyde(MDA)contents,lower catalase(CAT),peroxidase(POD)and superoxide dismutase(SOD)activities and reduced chlorophyll and soluble sugar contents under NaCl and PEG stress.In addition,the expression levels of six stress marker genes(Gh DREB2A,Gh SOS1,Gh CIPK6,Gh SOS2,Gh WRKY33,and Gh RD29A)were significantly downregulated after stress in the Gh ABC1K2-A05-and Gh ABC1K12-A07-silenced lines.The results indicate that knockdown of Gh ABC1K2-A05 and Gh ABC1K12-A07 make cotton more sensitive to salt and PEG stress.These findings can provide valuable information for intensive studies of Gh ABC1Ks in the responses and resistance of cotton to abiotic stresses.

Nonlinear coupling modeling and dynamics analysis of rotating flexible beams with stretching deformation effect

Dynamic coupling modeling and analysis of rotating beams based on the nonlinear Green-Lagrangian strain are introduced in this work.With the reservation of the axial nonlinear strain,there are more coupling terms for axial and transverse deformations.The discretized dynamic governing equations are obtained by using the finite element method and Lagrange’s equations of the second kind.Time responses are conducted to compare the proposed model with other previous models.The stretching deformation due to rotating motion is observed and calculated by special formulations under dynamic equilibrium.The stretching deformation and the change of the associated equilibrium position are taken into account to analyze the free vibration and frequency response of the rotating beams.Analytical and numerical comparisons show that the proposed model can provide reliable results,while the previous models may lead to imprecise results,especially in high-speed conditions.

Analysis of dynamic stress intensity factors of thick-walled cylinder under internal impulsive pressure

Dynamic stress intensity factors are evaluated for thick-walled cylinder with a radial edge crack under internal impulsive pressure. Firstly, the equation for stress intensity factors under static uniform pressure is used as the reference case, and then the weight function for a thick-walled cylinder containing a radial edge crack can be worked out. Secondly, the dynamic stresses in uncracked thick-walled cylinders are solved under internal impulsive pressure by using mode shape function method. The solution consists of a quasi-static solution satisfying inhomogeneous boundary conditions and a dynamic solution satisfying homogeneous boundary condi- tions, and the history and distribution of dynamic stresses in thick-walled cylinders are derived in terms of Fourier-Bessel series. Finally, the dynamic stress intensity factor equations for thick-walled cylinder containing a radial edge crack sub- jected to internal impulsive pressure are given by dynamic weight function method. The finite element method is utilized to verify the results of numerical examples, showing the validity and feasibility of the proposed method.

Rapid progression of nonculprit coronary lesions six weeks after successful primary PCI in culprit artery:a case report

We report a case of a 49-year-old man who was admitted with a 3-hour history of sudden onset of substemal chest pain. Coronary angiography revealed that the left circumflex artery （LCX） was acutely and totally occluded at the mid-portion. In addition, the proximal and mid-portion of the right coronary artery （RCA） had a 60% occlusion. We inferred that the LCX was the culprit artery and primary PCI was successfully performed. Six weeks later, the patient had an eventful course with recurrence of chest pain. Coronary angiography showed no significant ste- nosis in the previous LCX lesion, while the proximal and middle potion of the RCA had a 90% occlusion. Our case demonstrates the systemic nature of acute coronary syndromes and highlights the inherent instability of coronary artery disease.

Dynamic analysis of multi-link spatial flexible manipulator arms with dynamic stiffening effects

The dynamics for multi-link spatial flexible manipulator arms is investigated. The system considered here is an N-flexible-link manipulator driven by N DC-motors through N revolute flexiblejoints. The flexibility of each flexible joint is modeled as a linearly elastic torsional spring, and the mass of the joint is also considered. For the flexibility of the link, all of the stretching deformation, bending deformation and the torsional deformation are included. The complete governing equations of motion of the system are derived via the Lagrange equations. The nonlinear description of the deformation field of the flexible link is adopted in the dynamic modeling, and thus the dynamic stiffening effects are captured. Based on this model, a general-purpose software package for dynamic simulation of multi-link spatial flexible manipulator arms is developed. Several illustrative examples are given to validate the algorithm presented in this paper and to indicate that not only dynamic stiffening effects but also the flexibility of the structure has significant influence on the dynamic performance of the manipulator.

A simple practical balloon anchoring technique within the guide catheter for chronic total occlusion (CTO) of the coronary artery

Dear Editor： Chronic total occlusions （CTOs） of the coronary artery are commonly encountered complex lesionst11. Percutaneous coronary intervention （PCI） for CTO is technically challenging due to low procedural success ratesTM. Microcatheter is one of the important devices for treatment of CTOTM. It has been widely used attributed to the excellent crossability whenever angula- tion and tortuousity of the coronary artery is encoun- tered. In the process, the microcatheter has to be withdrawn from the guide wire after the wire is proved to locate in the true lumen.

Finite Element Analysis on Vibration Characteristics of an Offshore Floating Breakwater

The construction of seaside facilities is a hot topic in the field of ocean engineering.In this paper,a new type of floating breakwater is designed by 3D-CAD geometric modeling.Based on the vibration theory and finite element tech-nology,the floating breakwater model is optimized,and the modal analysis of the structure with the bracket as main body and blades as functional attachments is carried out.Natural frequencies and mode shapes of the blades are fi rst calculated,and the effects of the natural frequencies in both dry and wet conditions are taken into account.Modal analysis and harmonic response analysis of the bracket with different lengths by removing the blades are also carried out,and the different var-iations of the natural frequencies between several bracket units are compared.The responses of the key position of the bracket under different loads and different bracket lengths are analyzed.The influence of liquid on the natural frequency of the blades and the influence of the length of the bracket on the natural fre-quency of the bracket are discussed in the fluid-solid coupling state.Research in this paper provides a data basis for the safety assessment of the breakwater construction.

Hybrid vibration isolation optimization of a flexible manipulator based on neural network agent model

Segmented Active Constrained Layer Damping(SACLD)is an intelligent vibration-damping structure,which could be applied to the sectors of aviation,aerospace,and transportation engineering to reduce the vibration of flexible structures.Moreover,machine learning technology is widely used in the engineering field because of its efficient multi-objective optimization.The dynamic simulation of a rotational segmental flexible manipulator system is presented,in which enhanced active constrained layer damping is carried out,and the neural network model of Genetic Algorithm-Back Propagation(GA-BP)algorithm is investigated.Vibration suppression and structural optimization of the SACLD manipulator model are studied based on vibration mode and damping prediction.The modal responses of the SACLD manipulator model at rest and rotation are obtained.In addition,the four model indices are optimized using the GA-BP neural network:axial incision size,axial incision position,circumferential incision size,and circumferential incision position.Finally,the best model for vibration suppression is obtained.

Failure mechanisms of bolted flanges in aero-engine casings subjected to impact loading

In this paper,a failure evaluation criterion was proposed for the bolted casing-flange structure under impact loading.Subsequently,ballistic tests with eighteen bolted casing-flange structure specimens were conducted to validate the failure evaluation criterion.Parameter studies were then carried out using the validated FE models.Both the experimental and numerical results demonstrated the accuracy of the failure evaluation criterion.The failure evaluation criterion provided a quick and easy way to determine the failure mode of the casing connection area by using the materials and dimensions of the structure.Based on the failure evaluation criterion,designing the structural failure mode of the bolted casing-flange structure to be between flange failure and bolt failure can improve the impact resistance of the connection area of the aero-engine casings.This investigation revealed that the impact failure is not the unique criterion in evaluating the containment of the casing connection area,structural failure should also be involved in the evaluation criteria.