The bacterial pathogen Ralstonia solanacearum(R.solanacearum)delivered type III secretion effectors to inhibit the immune system and cause bacterial wilt on potato.Protein phosphatases are key regulators in plant immu...The bacterial pathogen Ralstonia solanacearum(R.solanacearum)delivered type III secretion effectors to inhibit the immune system and cause bacterial wilt on potato.Protein phosphatases are key regulators in plant immunity,which pathogens can manipulate to alter host processes.Here,we show that a type III effector RipAS can reduce the nucleolar accumulation of a type one protein phosphatase(PP1)StTOPP6 to promote bacterial wilt.StTOPP6 was used as bait in the Yeast two-Hybrid(Y2H)assay and acquired an effector RipAS that interacts with it.RipAS was characterized as a virulence effector to contribute to R.solanacearum infection,and stable expression of RipAS in potato impaired plant resistance against R.solanacearum.Overexpression of StTOPP6 showed enhanced disease symptoms when inoculated with wild strain UW551 but not the ripAS deletion mutant,indicating that the expression of StTOPP6 facilitates the virulence of RipAS.RipAS reduced the nucleolar accumulation of StTOPP6,which occurred during R.solanacearum infection.Moreover,the association also widely existed between other PP1s and RipAS.We argue that RipAS is a virulence effector associated with PP1s to promote bacterial wilt.展开更多
基金This work was funded by the National Natural Science Foundation of China(32201789)the China Agriculture Research System of MOF and MARA(CARS-09).
文摘The bacterial pathogen Ralstonia solanacearum(R.solanacearum)delivered type III secretion effectors to inhibit the immune system and cause bacterial wilt on potato.Protein phosphatases are key regulators in plant immunity,which pathogens can manipulate to alter host processes.Here,we show that a type III effector RipAS can reduce the nucleolar accumulation of a type one protein phosphatase(PP1)StTOPP6 to promote bacterial wilt.StTOPP6 was used as bait in the Yeast two-Hybrid(Y2H)assay and acquired an effector RipAS that interacts with it.RipAS was characterized as a virulence effector to contribute to R.solanacearum infection,and stable expression of RipAS in potato impaired plant resistance against R.solanacearum.Overexpression of StTOPP6 showed enhanced disease symptoms when inoculated with wild strain UW551 but not the ripAS deletion mutant,indicating that the expression of StTOPP6 facilitates the virulence of RipAS.RipAS reduced the nucleolar accumulation of StTOPP6,which occurred during R.solanacearum infection.Moreover,the association also widely existed between other PP1s and RipAS.We argue that RipAS is a virulence effector associated with PP1s to promote bacterial wilt.
