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不同测试体位对屈腕、屈指肌群定量肌力的影响及其标准化
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作者 杨泽人 高东 +3 位作者 夏晴 冉聃 盛延良 夏文涛 《法医学杂志》 CAS CSCD 北大核心 2024年第3期237-244,253,共9页
目的 探究不同测试体位对手、腕屈曲肌群定量肌力的影响,建立各肌群的标准化肌力检测方案。方法 征集40例健康受试者(男性12例,女性28例),使用MicroFET2TM便携式数字肌力测试仪,分别于腕关节背伸30°位、中立0°位和掌屈30°... 目的 探究不同测试体位对手、腕屈曲肌群定量肌力的影响,建立各肌群的标准化肌力检测方案。方法 征集40例健康受试者(男性12例,女性28例),使用MicroFET2TM便携式数字肌力测试仪,分别于腕关节背伸30°位、中立0°位和掌屈30°位测量各手指屈曲及腕关节掌屈肌力,分别于拇指掌侧外展30°位和60°位测量拇指掌侧外展肌力。从40例受试者中随机抽取10例,间隔10~15 d后由同一操作者再次测试各肌群定量肌力。结果 除男性腕关节背伸30°位和中立0°位之间的拇指屈曲肌力、腕关节掌屈肌力差异无统计学意义(P>0.05),各指屈曲及腕掌屈肌力值主要呈现以下特点:腕关节背伸30°位>腕关节中立0°位>腕关节掌屈30°位,拇指掌侧外展30°位>60°位;所有受试者手指屈曲肌力均为拇指>示指>中指>环指>小指;男性各项肌力值均大于女性(P<0.05);所有受试者左、右侧肌力值的差异均无统计学意义(P>0.05)。10例受试者不同时期测量肌力值的可靠性良好。结论 手、腕各肌群定量肌力均受体位影响,鉴定过程中应采取规范、统一的测试体位。MicroFET2TM用于手、腕关节定量肌力测试具有良好的可靠性。腕关节背伸30°位可作为各手指屈曲肌力及腕关节掌屈肌力的最佳标准化检测条件。拇指掌侧外展30°位可作为拇指掌侧外展肌力的最佳标准化检测条件。 展开更多
关键词 法医学 肌力 定量肌力 体位 手指屈曲 腕关节
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Real-time model correction using Kalman filter for Raman-controlled cell culture processes
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作者 Xiaoxiao dong Zhuohong He +5 位作者 Xu Yan dong gao Jingyu Jiao Yan Sun Haibin Wang Haibin Qu 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2024年第6期251-260,共10页
Raman spectroscopy has found extensive use in monitoring and controlling cell culture processes.In this context,the prediction accuracy of Raman-based models is of paramount importance.However,models established with ... Raman spectroscopy has found extensive use in monitoring and controlling cell culture processes.In this context,the prediction accuracy of Raman-based models is of paramount importance.However,models established with data from manually fed-batch cultures often exhibit poor performance in Raman-controlled cultures.Thus,there is a need for effective methods to rectify these models.The objective of this paper is to investigate the efficacy of Kalman filter(KF)algorithm in correcting Raman-based models during cell culture.Initially,partial least squares(PLS)models for different components were constructed using data from manually fed-batch cultures,and the predictive performance of these models was compared.Subsequently,various correction methods including the PLS-KF-KF method proposed in this study were employed to refine the PLS models.Finally,a case study involving the auto-control of glucose concentration demonstrated the application of optimal model correction method.The results indicated that the original PLS models exhibited differential performance between manually fed-batch cultures and Raman-controlled cultures.For glucose,the root mean square error of prediction(RMSEP)of manually fed-batch culture and Raman-controlled culture was 0.23 and 0.40 g·L^(-1).With the implementation of model correction methods,there was a significant improvement in model performance within Raman-controlled cultures.The RMSEP for glucose from updating-PLS,KF-PLS,and PLS-KF-KF was 0.38,0.36 and 0.17 g·L^(-1),respectively.Notably,the proposed PLS-KF-KF model correction method was found to be more effective and stable,playing a vital role in the automated nutrient feeding of cell cultures. 展开更多
关键词 Raman spectroscopy Model correction Algorithm Model-predictive control BIOPROCESS
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肝胆肿瘤中肿瘤特异性CircRNA衍生抗原肽的鉴定
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作者 Wenwen Wang Lili Ma +14 位作者 Zheng Xing Tinggan Yuan Jinxia Bao Yanjing Zhu Xiaofang Zhao Yan Zhao Yali Zong Yani Zhang Siyun Shen Xinyao Qiu Shuai Yang 王红阳 高栋 王鹏 陈磊 《Engineering》 SCIE EI CAS CSCD 2023年第3期159-170,共12页
基于肿瘤抗原的免疫治疗的应用受到验证免疫原性肽稀缺性的阻碍。本研究旨在研究环状RNA(circRNA)在肝胆肿瘤类器官中作为肿瘤抗原肽新来源的潜力。使用RNA测序(RNA-seq)和基于算法的评分工具,预测3950个翻译的肿瘤特异性环状RNA在27个... 基于肿瘤抗原的免疫治疗的应用受到验证免疫原性肽稀缺性的阻碍。本研究旨在研究环状RNA(circRNA)在肝胆肿瘤类器官中作为肿瘤抗原肽新来源的潜力。使用RNA测序(RNA-seq)和基于算法的评分工具,预测3950个翻译的肿瘤特异性环状RNA在27个类器官中产生18971个抗原肽。从抗原格局来看,11个氨基酸长度(mer)肽和人白细胞抗原(HLA)-A结合肽具有最高的免疫原性相关评分。在分析的3/5类器官中,有13个预测抗原肽通过质谱(MS)免疫肽组学被直接确认为HLA-A、HLA-B和HLA-C(HLA-ABC)结合肽。在流式细胞术和酶联免疫吸附试验(ELISA)中,由HLA-ABC分子呈递的circRNA衍生的肿瘤特异性肽刺激CD8(CD8)T细胞,显示CD107a干扰素γ(IFNγ)共表达和IFNγ分泌增加。免疫原性环状RNA衍生肽诱导的靶向类器官的细胞毒性T细胞活性在杀伤实验中得到验证。值得注意的是,来自circTBC1D15的抗原肽YGFNEILKK不仅被认为是类器官的HLA-ABC呈递肽,而且还显著降低了肿瘤类器官的存活率。本研究的发现强调了产生肿瘤抗原的一个关键亚群,这对靶向肿瘤特异性circRNA具有重要意义。 展开更多
关键词 Tumor antigen Patient-derived hepatobiliary tumor organoid Circular RNA Mass-spectrometry-based immunopeptidomics
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Negative feedback regulation of cellular antiviral signaling by RBCKl-mediated degradation of IRF3 被引量:13
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作者 Min Zhang Yang Tian +6 位作者 Rui-Peng Wang dong gao Yan Zhang Fei-Ci Diao Dan-Ying Chen Zhong-HeZhai Hong-Bing Shu 《Cell Research》 SCIE CAS CSCD 2008年第11期1096-1104,共9页
Viral infection causes host cells to produce type I interferons (IFNs), which are critically involved in viral clearance. Previous studies have demonstrated that activation of the transcription factor interferon reg... Viral infection causes host cells to produce type I interferons (IFNs), which are critically involved in viral clearance. Previous studies have demonstrated that activation of the transcription factor interferon regulatory factor (IRF)3 is essential for virus-triggered induction of type I IFNs. Here we show that the E3 ubiquitin ligase RBCC protein interacting with PKC1 (RBCK1) catalyzes the ubiquitination and degradation of IRF3. Overexpression of RBCK1 negatively regulates Sendai virus-triggered induction of type I IFNs, while knockdown of RBCK1 has the opposite effect. Plaque assays consistently demonstrate that RBCKI negatively regulates the cellular antiviral response. Furthermore, viral infection leads to induction of RBCK1 and subsequent degradation of IRF3. These findings suggest that the cellular antiviral response is controlled by a negative feedback regulatory mechanism involving RBCKl-mediated ubiquitination and degradation of IRF3. 展开更多
关键词 RBCK1 IRF3 antiviral response type I IFNs feedback regulation E3 ligase
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Optimization of Cutting Parameters for Trade-off Among Carbon Emissions, Surface Roughness, and Processing Time 被引量:4
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作者 Zhipeng Jiang dong gao +1 位作者 Yong Lu Xianli Liu 《Chinese Journal of Mechanical Engineering》 SCIE EI CAS CSCD 2019年第6期124-141,共18页
As the manufacturing industry is facing increasingly serious environmental problems, because of which carbon tax policies are being implemented, choosing the optimum cutting parameters during the machining process is ... As the manufacturing industry is facing increasingly serious environmental problems, because of which carbon tax policies are being implemented, choosing the optimum cutting parameters during the machining process is crucial for automobile panel dies in order to achieve synergistic minimization of the environment impact, product quality, and processing efficiency. This paper presents a processing task-based evaluation method to optimize the cutting parameters, considering the trade-off among carbon emissions, surface roughness, and processing time. Three objective models and their relationships with the cutting parameters were obtained through input–output, response surface, and theoretical analyses, respectively. Examples of cylindrical turning were applied to achieve a central composite design(CCD), and relative validation experiments were applied to evaluate the proposed method. The experiments were conducted on the CAK50135 di lathe cutting of AISI 1045 steel, and NSGA-Ⅱ was used to obtain the Pareto fronts of the three objectives. Based on the TOPSIS method, the Pareto solution set was ranked to find the optimal solution to evaluate and select the optimal cutting parameters. An S/N ratio analysis and contour plots were applied to analyze the influence of each decision variable on the optimization objective. Finally, the changing rules of a single factor for each objective were analyzed. The results demonstrate that the proposed method is effective in finding the trade-off among the three objectives and obtaining reasonable application ranges of the cutting parameters from Pareto fronts. 展开更多
关键词 Automobile panel dies Carbon emission Parameter optimization Multi-objective optimization NSGA-Ⅱ
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Conjugation of toll-like receptor-7 agonist to gastric cancer antigen MG7-Ag exerts antitumor effects 被引量:3
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作者 Xiao-dong Wang Ning-Ning gao +6 位作者 Yu-Wen Diao Yu Liu dong gao Wang Li Yan-Yan Wan Jing-Jing Zhong Guang-Yi Jin 《World Journal of Gastroenterology》 SCIE CAS 2015年第26期8052-8060,共9页
AIM: To investigate the effects of our tumor vaccines on reversing immune tolerance and generating therapeutic response.METHODS: Vaccines were synthesized by solid phase using an Fmoc strategy,where a small molecule t... AIM: To investigate the effects of our tumor vaccines on reversing immune tolerance and generating therapeutic response.METHODS: Vaccines were synthesized by solid phase using an Fmoc strategy,where a small molecule toll-like receptor-7 agonist(T7) was conjugated to a monoclonal gastric cancer 7 antigen mono-epitope(T7-MG1) or tri-epitope(T7-MG3).Cytokines were measured in both mouse bone marrow dendritic cells and mouse spleen lymphocytes after exposed to the vaccines.BALB/c mice were intraperitoneally immunized with the vaccines every 2 wk for a total of three times,andthen subcutaneously challenged with Ehrlich ascites carcinoma(EAC) cells.Three weeks later,the mice were killed,and the tumors were surgically removed and weighed.Serum samples were collected from the mice,and antibody titers were determined by ELISA using an alkaline phosphate-conjugated detection antibody for total Ig G.Antibody-dependent cell-mediated cytotoxicity was detected by the lactate dehydrogenase method using natural killer cells as effectors and antibody-labeled EAC cells as targets.Cytotoxic T lymphocyte activities were also detected by the lactate dehydrogenase method using lymphocytes as effectors and EAC cells as targets.RESULTS: Vaccines were successfully synthesized and validated by analytical high performance liquid chromatography and electrospray mass spectrometry,including T7,T7-MG1,and T7-MG3.Rapid inductions of tumor necrosis factor-α and interleukin-12 in bone marrow dendritic cells and interferon γ and interleukin-12 in lymphocytes occurred in vitro after T7,T7-MG1,and T7-MG3 treatment.Immunization with T7-MG3 reduced the EAC tumor burden in BALB/c mice to 62.64% ± 5.55% compared with PBS control(P < 0.01).Six or nine weeks after the first immunization,the monoclonal gastric cancer 7 antigen antibody increased significantly in the T7-MG3 group compared with the PBS control(P < 0.01).As for antibody-dependent cell-mediated cytotoxicity,antisera obtained by immunization with T7-MG3 were able to markedly enhance cell lysis compared to PBS control(31.58% ± 2.94% vs 18.02% ± 2.26%; P < 0.01).As for cytotoxic T lymphocytes,T7-MG3 exhibited obviously greater cytotoxicity compared with PBS control(40.92% ± 4.38% vs 16.29% ± 1.90%; P < 0.01).CONCLUSION: A successful method is confirmed for the design of gastric cancer vaccines by chemical conjugation of T7 and multi-repeat-epitope of monoclonal gastric cancer 7 antigen. 展开更多
关键词 Gastric CANCER Immunotherapy Monoclonalgastric CANCER 7 ANTIGEN TOLL-LIKE receptor-7 Vaccine
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A newly developed ocean significant wave height retrieval method from Envisat ASAR wave mode imagery 被引量:2
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作者 Chenqing Fan Xiaochen Wang +1 位作者 Xudong Zhang dong gao 《Acta Oceanologica Sinica》 SCIE CAS CSCD 2019年第9期120-127,共8页
The main objective of this paper is to propose a newly developed ocean Significant Wave Height (SWH) retrieval method from Envisat Advanced Synthetic Aperture Radar (ASAR) imagery. A series of wave mode imagery from J... The main objective of this paper is to propose a newly developed ocean Significant Wave Height (SWH) retrieval method from Envisat Advanced Synthetic Aperture Radar (ASAR) imagery. A series of wave mode imagery from January, April and May of 2011 are collocated with ERA-Interim reanalysis SWH data. Based on the matched datasets, a simplified empirical relationship between 22 types of SAR imagery parameters and SWH products is developed with the Genetic Algorithms Partial Least-Squares (GA-PLS) model. Two major features of the backscattering coefficient σ0 and the frequency parameter S10 are chosen as the optimal training feature subset of SWH retrieval by using cross validation. In addition, we also present a comparison of the retrieval results of the simplified empirical relationship with the collocated ERA-Interim data. The results show that the assessment index of the correlation coefficient, the bias, the root-mean-square error of cross validation (RMSECV) and the scattering index (SI) are 0.78, 0.07 m, 0.76 m and 0.5, respectively. In addition, the comparison of the retrieved SWH data between our simplifying model and the Jason-2 radar altimeter data is proposed in our study. Moreover, we also make a comparison of the retrieval of SWH data between our developed model and the well- known CWAVE_ENV model. The results show that satisfying retrieval results are acquired in the low-moderate sea state, but major bias appears in the high sea state, especially for SWH>5 m. 展开更多
关键词 SIGNIFICANT wave height ENVISAT ASAR GA-PLS optimal feature SUBSET
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Multiwavelength study of nearly face-on low surface brightness disk galaxies 被引量:1
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作者 dong gao Yan-Chun Liang +7 位作者 Shun-Fang Liu Guo-Hu Zhong Xiao-Yan Chen Yan-Bin Yang Francois Hammer Guo-Chao Yang Li-Cai Deng Jing-Yao Hu 《Research in Astronomy and Astrophysics》 SCIE CAS CSCD 2010年第12期1223-1241,共19页
We study the ages of a large sample (1802) of nearly face-on disk low surface brightness galaxies (LSBGs) using the evolutionary population synthesis (EPS) model PEGASE with an exponentially decreasing star form... We study the ages of a large sample (1802) of nearly face-on disk low surface brightness galaxies (LSBGs) using the evolutionary population synthesis (EPS) model PEGASE with an exponentially decreasing star formation rate to fit their mul- tiwavelength spectral energy distributions (SEDs) from far-ultraviolet (FUV) to nearinfrared (NIR). The derived ages of LSBGs are 1-5 Gyr for most of the sample no matter if constant or varying dust extinction is adopted, which are similar to most of the previous studies on smaller samples. This means that these LSBGs formed the majority of their stars quite recently. However, a small part of the sample (~2%-3%) has larger ages of 5-8 Gyr, meaning their major star forming process may have occurred earlier. At the same time, a large sample (5886) of high surface brightness galaxies (HSBGs) are selected and studied using the same method for comparisons. The de- rived ages are 1-5 Gyr for most of the sample (97%) as well. These results probably mean that these LSBGs have not much different star formation histories from their HSBGs counterparts. However, we should notice that the HSBGs are generally about 0.2 Gyr younger, which could mean that the HSBGs may have undergone more recent star forming activities than the LSBGs. 展开更多
关键词 galaxies: evolution -- galaxies: formation -- galaxies: photometry --galaxies: spiral -- galaxies: statistics -- ultraviolet: galaxies
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The 50 nm-thick yttrium iron garnet films with perpendicular magnetic anisotropy
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作者 Shuyao Chen Yunfei Xie +11 位作者 Yucong Yang dong gao donghua Liu Lin Qin Wei Yan Bi Tan Qiuli Chen Tao Gong En Li Lei Bi Tao Liu Longjiang Deng 《Chinese Physics B》 SCIE EI CAS CSCD 2022年第4期67-72,共6页
Yttrium iron garnet(YIG) films possessing both perpendicular magnetic anisotropy(PMA) and low damping would serve as ideal candidates for high-speed energy-efficient spintronic and magnonic devices.However,it is still... Yttrium iron garnet(YIG) films possessing both perpendicular magnetic anisotropy(PMA) and low damping would serve as ideal candidates for high-speed energy-efficient spintronic and magnonic devices.However,it is still challenging to achieve PMA in YIG films thicker than 20 nm,which is a major bottleneck for their development.In this work,we demonstrate that this problem can be solved by using substrates with moderate lattice mismatch with YIG so as to suppress the excessive strain-induced stress release as increasing the YIG thickness.After carefully optimizing the growth and annealing conditions,we have achieved out-of-plane spontaneous magnetization in YIG films grown on sGGG substrates,even when they are as thick as 50 nm.Furthermore,ferromagnetic resonance and spin pumping induced inverse spin Hall effect measurements further verify the good spin transparency at the surface of our YIG films. 展开更多
关键词 SPINTRONICS perpendicular magnetic anisotropy magnetic thin film deposition by sputtering
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化工清洁生产审核工作的研究
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作者 高东 李万鹏 《工程设计与施工》 2020年第10期41-43,共3页
化工行业在对社会进步、经济发展做出巨大贡献的同时,也对人类健康和生态环境构成极大威胁。化工行业要想获得健康持续发展,就要对清洁生产审核工作引起重视,以妥善解决化工行业快速发展与环境保护之间存在的矛盾。论文也联系清洁生产... 化工行业在对社会进步、经济发展做出巨大贡献的同时,也对人类健康和生态环境构成极大威胁。化工行业要想获得健康持续发展,就要对清洁生产审核工作引起重视,以妥善解决化工行业快速发展与环境保护之间存在的矛盾。论文也联系清洁生产审核内涵和程序,对化工清洁生产审核涉及到的企业准备、预审核、方案制定、选择实施等内容加以阐述。为了真正实现节能降耗、减污增效目标,还需要对技术设备升级改造、企业管理、废物循环利用等要点进行关注,以推动化工行业健康长远发展。 展开更多
关键词 化工行业 清洁生产 审核工作 研究
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Mild synthesis of ultra-bright carbon dots with solvatochromism for rapid lipid droplet monitoring in varied physiological processes
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作者 Borui Su dong gao +10 位作者 Nini Xin Kai Wu Mei Yang Shichao Jiang Yusheng Zhang Jie Ding Chengheng Wu Jing Sun Dan Wei Hongsong Fan Zhenzhen Guo 《Regenerative Biomaterials》 SCIE EI CSCD 2024年第3期30-42,共13页
Lipid droplets(LDs)participating in various cellular activities and are increasingly being emphasized.Fluorescence imaging provides powerful tool for dynamic tracking of LDs,however,most current LDs probes remain inco... Lipid droplets(LDs)participating in various cellular activities and are increasingly being emphasized.Fluorescence imaging provides powerful tool for dynamic tracking of LDs,however,most current LDs probes remain inconsistent performance such as low Photoluminescence Quantum Yield(PLQY),poor photostability and tedious washing procedures.Herein,a novel yellow-emissive carbon dot(OT-cD)has been synthesized conveniently with high PLQY up to 90%.Besides,OT-CD exhibits remarkable amphiphilicity and solvatochromic property with lipid-water partition coefficient higher than 2,which is much higher than most LDs probes.These characters enable OT-CD high brightness,stable and wash-free LDs probing,and feasible for in vivo imaging.Then,detailed observation of LDs morphological and polarity variation dynamically in different cellular states were recorded,including ferroptosis and other diseases processes.Furthermore,fast whole imaging of zebrafish and identifed LD enrichment in injured liver indicate its further feasibility for in vivo application.In contrast to the reported studies to date,this approach provides a versatile conventional synthesis system for high-performance LDs targeting probes,combing the advantages of easy and high-yield production,as well as robust brightness and stability for long-term imaging,facilitating investigations into organelle interactions and LD-associated diseases. 展开更多
关键词 lipid droplet probe carbon dots fluorescence imaging wash-free dynamic monitoring
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On hypersurfaces of H^(2)×H^(2)
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作者 dong gao Hui Ma Zeke Yao 《Science China Mathematics》 SCIE CSCD 2024年第2期339-366,共28页
In this paper,we study hypersurfaces of H^(2)×H^(2).We first classify the hypersurfaces with constant principal curvatures and constant product angle functions.Then we classify homogeneous hypersurfaces and isopa... In this paper,we study hypersurfaces of H^(2)×H^(2).We first classify the hypersurfaces with constant principal curvatures and constant product angle functions.Then we classify homogeneous hypersurfaces and isoparametric hypersurfaces,respectively.Finally,we classify the hypersurfaces with at most two distinct constant principal curvatures,as well as those with three distinct constant principal curvatures under some additional conditions. 展开更多
关键词 constant principal curvature homogeneous hypersurface isoparametric hypersurface
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Recent advances in F-containing iodate nonlinear optical materials
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作者 dong gao Hongping Wu +3 位作者 Zhanggui Hu Jiyang Wang Yicheng Wu Hongwei Yu 《Chinese Journal of Structural Chemistry》 SCIE CAS CSCD 2023年第1期30-45,共16页
Metal iodates containing I(Ⅴ)with lone electron pairs in an asymmetric coordination geometry exhibit excellent second-harmonic generation(SHG)responses,wide transmission range and large band gaps.During the past ten ... Metal iodates containing I(Ⅴ)with lone electron pairs in an asymmetric coordination geometry exhibit excellent second-harmonic generation(SHG)responses,wide transmission range and large band gaps.During the past ten years,metal iodates have been widely studied,and introducing large electronegative fluorine atoms into metal iodates has also attracted much attention.These F-containing iodates are different from pure iodates in terms of composition,symmetry and transmittance.Especially because oxide-fluoride heterolepic anion groups can exhibit interesting structure-directing properties,e.g.trans-and cis-directing properties,these F-containing iodates also have better structure designability.Therefore,in this review,we will review the research progress of F-containing iodates and address the effect of structure-directing properties of oxide-fluoride anions on the crystal structures of F-containing iodates.Since the valence of the central cations will greatly affect the distortions and configurations of the oxide-fluoride anion groups,these F-containing iodates will be classified and introduced according to the different valences of the central cations. 展开更多
关键词 MID-INFRARED Nonlinear optical materials Iodate-fluorides Fluoroiodates
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Cell fate determination and lineage plasticity in prostate cancer
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作者 dong gao 《Asian Journal of Andrology》 SCIE CAS CSCD 2023年第2期149-151,F0002,共4页
Prostate cancer(PCa)initiation,progression,and therapy resistance involve genetic and epigenetic alterations that l e ad to ab err ant cel l line age specification and plasticity.1–3 The vast majority of primary pros... Prostate cancer(PCa)initiation,progression,and therapy resistance involve genetic and epigenetic alterations that l e ad to ab err ant cel l line age specification and plasticity.1–3 The vast majority of primary prostate cancers are pathologically defined as luminal cancer with luminal cell expansion and absence of basal cells. 展开更多
关键词 alterations CANCER PLASTICITY
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Recent advances in tissue stem cells 被引量:3
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作者 Xin Fu Qiang He +12 位作者 Yu Tao Mengdi Wang Wei Wang Yalong Wang Qing Cissy Yu Fang Zhang Xiaoyu Zhang Ye-Guang Chen dong gao Ping Hu Lijian Hui Xiaoqun Wang Yi Arial Zeng 《Science China(Life Sciences)》 SCIE CAS CSCD 2021年第12期1998-2029,共32页
Stem cells are undifferentiated cells capable of self-renewal and differentiation,giving rise to specialized functional cells.Stem cells are of pivotal importance for organ and tissue development,homeostasis,and injur... Stem cells are undifferentiated cells capable of self-renewal and differentiation,giving rise to specialized functional cells.Stem cells are of pivotal importance for organ and tissue development,homeostasis,and injury and disease repair.Tissue-specific stem cells are a rare population residing in specific tissues and present powerful potential for regeneration when required.They are usually named based on the resident tissue,such as hematopoietic stem cells and germline stem cells.This review discusses the recent advances in stem cells of various tissues,including neural stem cells,muscle stem cells,liver progenitors,pancreatic islet stem/progenitor cells,intestinal stem cells,and prostate stem cells,and the future perspectives for tissue stem cell research. 展开更多
关键词 neural stem cells muscle stem cells liver progenitors pancreatic islet stem/progenitor cells intestinal stem cells prostate stem cells
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Resolution enhancement in hydrophobic interaction chromatography via electrostatic interactions 被引量:4
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作者 dong gao Fu-Chun Tan +1 位作者 Wen-Peng Wang Li-Li Wang 《Chinese Chemical Letters》 SCIE CAS CSCD 2013年第5期419-421,共3页
In this work,a new type of hydrophobic stationary phase that provide electrostatic interactions with analytes was developed by bondingβ-phenylethylamine as a functional ligand to silica.This stationary phase can sepa... In this work,a new type of hydrophobic stationary phase that provide electrostatic interactions with analytes was developed by bondingβ-phenylethylamine as a functional ligand to silica.This stationary phase can separate proteins with similar hydrophobicity that traditional hydrophobic resins cannot.Hen egg white was separated to examine the selectivity.The results show that the introduced electrostatic interactions are an important factor for the resolution enhancement and the new resin could have important applications in separation and purification of biological macromolecules. 展开更多
关键词 Hydrophobic interaction chromatography Electrostatic interaction RESOLUTION Hen egg white
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Organoid technology for tissue engineering 被引量:2
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作者 Juan He Xiaoyu Zhang +5 位作者 Xinyi Xia Ming Han Fei Li Chunfeng Li Yunguang Li dong gao 《Journal of Molecular Cell Biology》 SCIE CAS CSCD 2020年第8期569-579,共11页
For centuries,attempts have been continuously made to artificially reconstitute counterparts of in vivo organs from their tissues or cells.Only in the recent decade has organoid technology as a whole technological fie... For centuries,attempts have been continuously made to artificially reconstitute counterparts of in vivo organs from their tissues or cells.Only in the recent decade has organoid technology as a whole technological field systematically emerged and been shown to play important roles in tissue engineering.Based on their self-organizing capacities,stem cells of versatile organs,both harvested and induced,can form 3D structures that are structurally and functionally similar to their in vivo counterparts.These organoid models provide a powerful platform for elucidating the development mechanisms,modeling diseases,and screening drug candidates.In this review,we will summarize the advances of this technology for generating various organoids of tissues from the three germ layers and discuss their drawbacks and prospects for tissue engineering. 展开更多
关键词 organoid tissue engineering 3D culture
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Differentiation of storage time of wheat seed based on near infrared hyperspectral imaging 被引量:1
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作者 dong gao Guo Jian +4 位作者 Wang Cheng Liang Kehong Lu Lingang Wang Jing Zhu Dazhou 《International Journal of Agricultural and Biological Engineering》 SCIE EI CAS 2017年第2期251-258,共8页
Seed aging during storage is one of the main factors that influence the quality of wheat seed.Current detection methods based on NIR spectra were mostly for group seeds,they had poor stability for single seed detectio... Seed aging during storage is one of the main factors that influence the quality of wheat seed.Current detection methods based on NIR spectra were mostly for group seeds,they had poor stability for single seed detection because of sample uniformity.In this study,the characteristic changes of single wheat seed during storage procedure were measured through hyperspectral imaging technology.Firstly,hyperspectral imaging data of wheat grain including six years from 2007 to 2012 had been collected.The original spectra showed clear difference in the band of 1400-1600 nm,which may be caused by the decreasing of moisture and protein content during storage;principal component analysis(PCA)was applied to analyze the spectral data of wheat grain including six years,the clustering chart of the principal components indicated that the grain between same or similar year have an clustering characteristic,and the characteristic difference would become obviously with the increasing of storage time;soft independent modeling of class analogy(SIMCA)was applied to classify the grain of different years,results showed that the classification accuracy of the dichotomy between adjacent years could reach 97.05%,and the accuracy of the mixed classification of six years could also reach 82.5%.These results indicated that hyperspectral imaging technology could be used to differentiate the quality change of wheat seed during different storage time,which could provide support for the intelligent monitoring of stored wheat seeds. 展开更多
关键词 hyperspectral image wheat seed STORAGE intelligent monitoring single seed
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Strategies for Inhibition and Disaggregation of Amyloid-β Fibrillation
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作者 Hao Geng dong gao +3 位作者 Zijuan Wang Xiaoning Liu Zhanshuo Cao Chengfen Xing 《Chinese Journal of Chemistry》 SCIE CAS CSCD 2022年第4期524-538,共15页
Comprehensive Summary Amyloid-β protein(Aβ)is a fatal cause of Alzheimer's disease,which can trigger a series of cytotoxicity by the abnormal aggregation of Aβ in human brain.The strategies for inhibition and d... Comprehensive Summary Amyloid-β protein(Aβ)is a fatal cause of Alzheimer's disease,which can trigger a series of cytotoxicity by the abnormal aggregation of Aβ in human brain.The strategies for inhibition and disaggregation of Aβ fibrillation are mostly based on the interaction between monomers,oligomers,fibrils,and materials. 展开更多
关键词 Amyloid beta-peptides Inhibition and disaggregation Materials science PHOTOOXIDATION Photothermal effect
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A flexible and high temperature tolerant strain sensor of La0.7Sr0.3MnO3/Mica
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作者 Min Guo Cheng Yang +10 位作者 dong gao Qiang Li Aihua Zhang Jiajun Feng Hui Yang Ruiqiang Tao Zhen Fan Min Zeng Guofu Zhou Xubing Lu J-MLiu 《Journal of Materials Science & Technology》 SCIE EI CAS CSCD 2020年第9期42-47,共6页
Flexible sensors have been widely investigated due to their broad application prospects in various flexible electronics.However,most of the presently studied flexible sensors are only suitable for working at room temp... Flexible sensors have been widely investigated due to their broad application prospects in various flexible electronics.However,most of the presently studied flexible sensors are only suitable for working at room temperature,and their applications at high or low temperatures are still a big challenge.In this work,we present a multimodal flexible sensor based on functional oxide La0.7Sr0.3MnO3(LSMO)thin film deposited on mica substrate.As a strain sensor,it shows excellent sensitivity to mechanical bending and high bending durability(up to 3600 cycles).Moreover,the LSMO/Mica sensor also shows a sensitive response to the magnetic field,implying its multimodal sensing ability.Most importantly,it can work in a wide temperature range from extreme low temperature down to 20K to high temperature up to 773 K.The flexible sensor based on the flexible LSMO/mica hetero-structure shows great potential applications for flexible electronics using at extreme temperature environment in the future. 展开更多
关键词 Flexible sensor MICA LA0.7SR0.3MNO3 High temperature Multimodal sensing
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