Te nitrocellulose(NC)membrane based lateral fow immunoassay device(LFID)is one of the most important and widely used biosensor platforms for point-of-care(PoC)diagnostics.However,the analytical performance of LFID has...Te nitrocellulose(NC)membrane based lateral fow immunoassay device(LFID)is one of the most important and widely used biosensor platforms for point-of-care(PoC)diagnostics.However,the analytical performance of LFID has limitations and its optimization is restricted to the bioassay chemistry,the membrane porosity,and the choice of biolabel system.Tese bottom neck technical issues resulted from the fact that the conventional LFID design principle has not evolved for many years,which limited the LFID for advanced biosensor applications.Here we introduce a new dimension for LFID design and optimization based on geometric fow control(GFC)of NC membranes,leading to highly sensitive GFC-LFID.Tis novel approach enables comprehensive fow control via diferent membrane geometric features such as the width(θ)and the length(l)of a constriction,as well as its input angle(θ_(1))and output angle(θ_(2)).Te GFC-LFID(θ=0.5 mm,l=7 mm,θ_(1)=60∘,θ_(2)=45∘)attained a 10-fold increase in sensitivity for detection of interleukin-6(IL-6),compared with conventional LFID,whereas reducing by 10-fold the antibody consumption.Te GFC-LFID detects IL-6 over a linear range of 0.1–10 ng/mL with a limit of detection(LoD)of 29 pg/mL,which even outperforms some commercial IL-6 LFIDs.Such signifcant improvement is attained by pure geometric control of the NC membrane,without additives,that only relaying on a simple high throughput laser ablation procedure suitable for integration on regular large-scale manufacturing of GFC-LFIDs.Our new development on GFC-LFID with the combination of facile scalable fabrication process,tailored fow control,improved analytical performance,and reduced antibodies consumption is likely to have a signifcant impact on new design concept for the LFID industry.展开更多
基金This work was supported by grants to D.Filippini from the Swedish Research Council(VR)(Grant no.C0453801)to W.C.Mak from Swedish Research Council(VR-2014-43058).
文摘Te nitrocellulose(NC)membrane based lateral fow immunoassay device(LFID)is one of the most important and widely used biosensor platforms for point-of-care(PoC)diagnostics.However,the analytical performance of LFID has limitations and its optimization is restricted to the bioassay chemistry,the membrane porosity,and the choice of biolabel system.Tese bottom neck technical issues resulted from the fact that the conventional LFID design principle has not evolved for many years,which limited the LFID for advanced biosensor applications.Here we introduce a new dimension for LFID design and optimization based on geometric fow control(GFC)of NC membranes,leading to highly sensitive GFC-LFID.Tis novel approach enables comprehensive fow control via diferent membrane geometric features such as the width(θ)and the length(l)of a constriction,as well as its input angle(θ_(1))and output angle(θ_(2)).Te GFC-LFID(θ=0.5 mm,l=7 mm,θ_(1)=60∘,θ_(2)=45∘)attained a 10-fold increase in sensitivity for detection of interleukin-6(IL-6),compared with conventional LFID,whereas reducing by 10-fold the antibody consumption.Te GFC-LFID detects IL-6 over a linear range of 0.1–10 ng/mL with a limit of detection(LoD)of 29 pg/mL,which even outperforms some commercial IL-6 LFIDs.Such signifcant improvement is attained by pure geometric control of the NC membrane,without additives,that only relaying on a simple high throughput laser ablation procedure suitable for integration on regular large-scale manufacturing of GFC-LFIDs.Our new development on GFC-LFID with the combination of facile scalable fabrication process,tailored fow control,improved analytical performance,and reduced antibodies consumption is likely to have a signifcant impact on new design concept for the LFID industry.
