Korean freshwater snails of the genus Semisulcospira are widely distributed across East Asia.It has been a very popular nutritional food in Korea,and is an ecologically important water quality indicator because it liv...Korean freshwater snails of the genus Semisulcospira are widely distributed across East Asia.It has been a very popular nutritional food in Korea,and is an ecologically important water quality indicator because it lives only in clean water.However,no microsatellite markers have been generated to study the population genetic diversity of this genus.In the present study,we developed and characterized 18 novel microsatellite loci from Semisulcospira coreana genomic DNA.The microsatellites were isolated using 454 GS-FLX titanium sequencing and 18 markers were used for genotyping in S.coreana.In addition,we also tested the cross-species transferability of the microsatellite markers in four additional Semisulcospira spp.We identified 18 polymorphic loci and the number of alleles per loci,and their polymorphism information content values ranged from 2 to 17 and 0.203 to 0.902,respectively.The observed and expected heterozygosities of the loci ranged from 0.063 to 0.924 and 0.226 to 0.924,respectively.According to the analysis of the cross-species transferability of these markers,four species,S.forticosta,S.gottschei,S.tegulata,and S.libertina,showed a very high transferability(80%–85%).These results show that this set of nuclear markers could be useful for population genetics studies of this species and closely related species.展开更多
The nestling diet of the Fairy Pitta (pitta nympha) was studied by videotaping during breeding period in Jeju Island, 2009. Earthworms of several species were the most common food resources for nestlings, averaging 82...The nestling diet of the Fairy Pitta (pitta nympha) was studied by videotaping during breeding period in Jeju Island, 2009. Earthworms of several species were the most common food resources for nestlings, averaging 82% of all items, followed by 4% of Homoptera larvae. The remaining was only rarely recorded, including Lepidopteran larvae and adults, slugs, spiders, beetle adults and larvae (Coleoptera) and grasshoppers. Adults provided the number of preys ranging from 1 to 7 items to chicks per one visit. The average value of prey number per visit was 3.0 (SD = 1.38). The estimated average length of prey was 5.7 cm (SD = 2.85), and 96% of the food items were smaller than 10 cm. The staying time for feeding in an early stage was longer than other stages. Provision rate at a forenoon (mean ± SD, 14.7 ± 4.92) and an afternoon time (15.8 ± 5.30) was not significantly higher than that of noon time (11.7 ± 4.49). These results provide basic information for conservation action of international endangered species of this species.展开更多
基金Supported by the National Institute of Fisheries Science of Republic of Korea(Nos.R2019030,R2019033)
文摘Korean freshwater snails of the genus Semisulcospira are widely distributed across East Asia.It has been a very popular nutritional food in Korea,and is an ecologically important water quality indicator because it lives only in clean water.However,no microsatellite markers have been generated to study the population genetic diversity of this genus.In the present study,we developed and characterized 18 novel microsatellite loci from Semisulcospira coreana genomic DNA.The microsatellites were isolated using 454 GS-FLX titanium sequencing and 18 markers were used for genotyping in S.coreana.In addition,we also tested the cross-species transferability of the microsatellite markers in four additional Semisulcospira spp.We identified 18 polymorphic loci and the number of alleles per loci,and their polymorphism information content values ranged from 2 to 17 and 0.203 to 0.902,respectively.The observed and expected heterozygosities of the loci ranged from 0.063 to 0.924 and 0.226 to 0.924,respectively.According to the analysis of the cross-species transferability of these markers,four species,S.forticosta,S.gottschei,S.tegulata,and S.libertina,showed a very high transferability(80%–85%).These results show that this set of nuclear markers could be useful for population genetics studies of this species and closely related species.
文摘The nestling diet of the Fairy Pitta (pitta nympha) was studied by videotaping during breeding period in Jeju Island, 2009. Earthworms of several species were the most common food resources for nestlings, averaging 82% of all items, followed by 4% of Homoptera larvae. The remaining was only rarely recorded, including Lepidopteran larvae and adults, slugs, spiders, beetle adults and larvae (Coleoptera) and grasshoppers. Adults provided the number of preys ranging from 1 to 7 items to chicks per one visit. The average value of prey number per visit was 3.0 (SD = 1.38). The estimated average length of prey was 5.7 cm (SD = 2.85), and 96% of the food items were smaller than 10 cm. The staying time for feeding in an early stage was longer than other stages. Provision rate at a forenoon (mean ± SD, 14.7 ± 4.92) and an afternoon time (15.8 ± 5.30) was not significantly higher than that of noon time (11.7 ± 4.49). These results provide basic information for conservation action of international endangered species of this species.
