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DUOX2对结直肠癌细胞氟尿嘧啶敏感性的影响 被引量:2
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作者 张雪 刘妍 +5 位作者 周欣亮 范志松 韩晶 冯莉 左静 王贵英 《中国肿瘤生物治疗杂志》 CAS CSCD 北大核心 2020年第12期1365-1371,共7页
目的:探讨双氧化酶2(dual oxidase 2,DUOX2)对结直肠癌(colorectal cancer,CRC)细胞5-氟尿嘧啶(5-fluorouracil,5-FU)药物敏感性的影响。方法:选用CRC细胞系DLD-1、SW480、HCT116、SW620与正常肠上皮细胞株NCM460,用qPCR法检测细胞中DU... 目的:探讨双氧化酶2(dual oxidase 2,DUOX2)对结直肠癌(colorectal cancer,CRC)细胞5-氟尿嘧啶(5-fluorouracil,5-FU)药物敏感性的影响。方法:选用CRC细胞系DLD-1、SW480、HCT116、SW620与正常肠上皮细胞株NCM460,用qPCR法检测细胞中DUOX2的表达水平。利用慢病毒感染技术,稳定敲降HT-29与HCT116细胞中DUOX2表达,qPCR法和WB法检测敲降效率。用不同浓度(0、5、10、20、40、80、120μg/ml)5-FU处理sh-Control组与sh-DUOX2组细胞,用CCK-8法、流式细胞术分别检测5-FU对细胞增殖、细胞凋亡和细胞周期的影响。构建裸鼠HT29细胞移植瘤模型,观察DUOX2基因对5-FU疗效的影响。结果:DUOX2 mRNA在CRC细胞中的表达水平显著高于NCM460细胞(P<0.05或P<0.01)。敲降DUOX2基因后,与sh-Control组相比,sh-DUOX2组HT29和HCT116细胞中DUOX2 mRNA和蛋白表达水平明显下降(均P<0.01);细胞对5-FU的敏感性增强,细胞凋亡率明显升高(均P<0.01),G0/G1期比例显著升高、G2期与S期比例显著下降(均P<0.01)。未经5-FU治疗的sh-Control组与sh-DUOX2组裸鼠移植瘤体积及质量比较差异均无统计学意义(均P>0.05),经5-FU治疗的sh-DUOX2+5-FU组移植瘤体积及质量明显低于sh-Control+5-FU组(均P<0.01)。结论:敲降DUOX2基因可显著增强CRC细胞对5-FU的敏感性。 展开更多
关键词 双氧化酶2 5-氟尿嘧啶 结直肠癌 HT29细胞 HCT116细胞 增殖 凋亡
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近惯性内波破碎混合方案对MOM4模式的影响
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作者 吴方华 范植松 楚合涛 《海洋学报》 CAS CSCD 北大核心 2019年第3期1-11,共11页
风生近惯性内波破碎引起的跨等密度面混合在海洋内部混合中起重要作用。然而其参数化对海洋模式的模拟影响仍有待进一步认识。本文给出的是在模块化海洋模式(MOM)中海洋表面边界层以下引入一个考虑风驱动近惯性内波破碎引起的跨等密度... 风生近惯性内波破碎引起的跨等密度面混合在海洋内部混合中起重要作用。然而其参数化对海洋模式的模拟影响仍有待进一步认识。本文给出的是在模块化海洋模式(MOM)中海洋表面边界层以下引入一个考虑风驱动近惯性内波破碎引起的跨等密度面混合参数化方案的研究工作。模拟结果显示,该方案有效改善MOM4模拟的上层1 000 m以上的温盐偏差,特别是在北太平洋和北大西洋的通风地区。数值试验表明,风生近惯性内波破碎有可能是维持海洋通风过程的重要机制之一,它使得海洋通风区的位温变冷,盐度变淡,整层等位密面加深。维持的通风过程使得北太平洋副极地大涡的影响延伸到副热带大涡。从而模拟的北太平洋中层水源头及其副热带大涡东侧的温盐更接近观测实际。同时,模拟的北大西洋经圈翻转环流强度也更为合理。 展开更多
关键词 跨等密度面混合 近惯性内波破碎 北太平洋中层水 大西洋翻转环流
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A study of internal solitary waves observed on the continental shelf in the northwestern South China Sea 被引量:18
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作者 XU Zhenhua YIN Baoshu +2 位作者 HOU Yijun fan zhisong Antony K LIU 《Acta Oceanologica Sinica》 SCIE CAS CSCD 2010年第3期18-25,共8页
Based on in-situ time series data from the acoustic Doppler current profiler (ADCP) and thermistor chain in Wenchang area, a sequence of internal solitary wave (ISW) packets was observed in September 2005, propaga... Based on in-situ time series data from the acoustic Doppler current profiler (ADCP) and thermistor chain in Wenchang area, a sequence of internal solitary wave (ISW) packets was observed in September 2005, propagating northwest on the continental shelf of the northwestern South China Sea (SCS). Corresponding to different stratification of the water column and tidal condition, both elevation and depression ISWs were observed at the same mooring location with amplitude of 35 m and 25 m respectively in different days. Regular arrival of the remarkable ISW packets at approximately the diurnal tidal period and the dominance of diurnal internal waves in the study area, strongly suggest that the main energy source of the waves is the diurnal tide. Notice that the wave packets were all riding on the troughs and shoulders of the internal tides, they were probably generated locally from the shelf break by the evolution of the internal tides due to nonlinear and dispersive effects. 展开更多
关键词 elevation internal solitary waves diurnal internal tide northwestern South China Sea
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A parameterization scheme of vertical mixing due to inertial internal wave breaking in the ocean general circulation model 被引量:4
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作者 fan zhisong SHANG Zhenqi +2 位作者 ZHANG Shanwu HU Ruijin LIU Hailong 《Acta Oceanologica Sinica》 SCIE CAS CSCD 2015年第1期11-22,共12页
Based on the theoretical spectral model of inertial internal wave breaking (fine structure) proposed previ- ously, in which the effects of the horizontal Coriolis frequency component f-tilde on a potential isopycnal... Based on the theoretical spectral model of inertial internal wave breaking (fine structure) proposed previ- ously, in which the effects of the horizontal Coriolis frequency component f-tilde on a potential isopycnal are taken into account, a parameterization scheme of vertical mixing in the stably stratified interior be- low the surface mixed layer in the ocean general circulation model (OGCM) is put forward preliminarily in this paper. Besides turbulence, the impact of sub-mesoscale oceanic processes (including inertial internal wave breaking product) on oceanic interior mixing is emphasized. We suggest that adding the inertial inter- hal wave breaking mixing scheme (F-scheme for short) put forward in this paper to the turbulence mixing scheme of Canuto et al. (T-scheme for short) in the OGCM, except the region from 15°S to 15°N. The numeri- cal results ofF-scheme by usingWOA09 data and an OGCM (LICOM, LASG/IAP climate system ocean model) over the global ocean are given. A notable improvement in the simulation of salinity and temperature over the global ocean is attained by using T-scheme adding F-scheme, especially in the mid- and high-latitude regions in the simulation of the intermediate water and deep water. We conjecture that the inertial internal wave breaking mixing and inertial forcing of wind might be one of important mechanisms maintaining the ventilation process. The modeling strength of the Atlantic meridional overturning circulation (AMOC) by using T-scheme adding F-scheme may be more reasonable than that by using T-scheme alone, though the physical processes need to be further studied, and the overflow parameterization needs to be incorporated. A shortcoming in F-scheme is that in this paper the error of simulated salinity and temperature by using T-scheme adding F-scheme is larger than that by using T-scheme alone in the subsurface layer. 展开更多
关键词 vertical mixing inertial internal wave fine structure horizontal Coriolis frequency component ocean general circulation model
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Simulation model of SAR remote sensing of turbulent wake of semi-elliptical submerged body 被引量:3
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作者 fan zhisong 《Acta Oceanologica Sinica》 SCIE CAS CSCD 2005年第5期50-60,共11页
In terms of the 2-dimensional hydrodynamic simplified model of a semi-elliptical submerged body moving horizontally at high speed, by using the full-spectrum model of SAR(synthetic aperture radar) remote sensing and... In terms of the 2-dimensional hydrodynamic simplified model of a semi-elliptical submerged body moving horizontally at high speed, by using the full-spectrum model of SAR(synthetic aperture radar) remote sensing and taking the effect of oceanic interior turbulence on surface gravity capillary waves into account, applying the k-ε model of turbulence with internal wave mixing, and adopthag the Nasmyth spectrum of oceanic turbulence, the 2-dimensional simulation model of SAR remote sensing of this semi-elliptical submerged body is built up. Simulation by using this model at X band and C band is made in the northeastern South China Sea (21°00' N, 119°00' E). Satisfactory results of the delay time and delay distance of turbulent surface wake of this semi-elliptical submerged body, as well as the minimum submerged depth at which this submerged body which cannot be discovered by SAR, are obtained through simulation. 展开更多
关键词 semi-elliptical submerged body SAR remote sensing WAKE TURBULENCE lee waves
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Effects of Tidal Currents on Nonlinear Internal Solitary Waves in the South China Sea 被引量:3
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作者 fan zhisong SHI Xingang +2 位作者 Antony K. Liu LIU Hailong LI Peiliang 《Journal of Ocean University of China》 SCIE CAS 2013年第1期13-22,共10页
The propagation and fission process of internal solitary waves (ISWs) with amplitudes of about 170 m are simulated in the northeast of the South China Sea (NSCS) by using the generalized Korteweg-de Vries (KdV) ... The propagation and fission process of internal solitary waves (ISWs) with amplitudes of about 170 m are simulated in the northeast of the South China Sea (NSCS) by using the generalized Korteweg-de Vries (KdV) equation under continuous stratification. More attention is paid to the effects of the ebb and flood background currents on the fission process of ISWs. This kind of background current is provided by the composed results simulated in terms of monthly mean baroclinic circulation and barotropic tidal current. It is found that the obtained relation of the number of fission solitons to the water depth and stratification is roughly in accordance with the fission law derived by Djordjevic and Redekopp in 1978; however, there exists obvious difference between the effects of the ebb and flood background currents on the wave-lengths of fission solitons (defined as the distance between two neighboring peaks of ISWs). The difference in nonlinearity coefficient a between the ebb and flood background currents is a main cause for the different wave-lengths of fission solitons. 展开更多
关键词 internal solitary waves tidal current fission process the South China Sea
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Is the small-scale turbulence an exclusive breaking product of oceanic internal waves 被引量:2
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作者 fan zhisong 《Acta Oceanologica Sinica》 SCIE CAS CSCD 2011年第6期1-11,共11页
On the basis of the theoretical research results by the author and the literature published up to date, the analysis and the justification presented in this paper show that the breaking products of oceanic internal wa... On the basis of the theoretical research results by the author and the literature published up to date, the analysis and the justification presented in this paper show that the breaking products of oceanic internal waves are not only turbulence, but also the fine-scale near-inertial internal waves (the oceanic reversible finestructure) for inertial waves and the internal solitary waves for internal tides respectively. It was found that the oceanic reversible finestructure may be induced by the effect of the horizontal component f (f = 2Ωcosφ) of the rotation vector on inertial waves. And a new instability of the theoretical shear and strain spectra due to the effect of f occurs at critical vertical wavenumber β c ≈ 0.1 cpm. It happens when the levels of shear and strain of the reversible finestructure are higher than those of inertial waves, which is induced by the effect of f along an "iso-potential-pycnal" of internal wave. If all breaking products of internal waves are taken into account, the average kinetic energy dissipation rate is an order of magnitude larger than the values of turbulence observed by microstructure measurements. The author’s theoretical research results are basically in agreement with those observed in IWEX, DRIFTER and PATCHEX experiments. An important impersonal fact is that on the mean temporal scale of thermohaline circulation these breaking products of internal waves exist simultaneously with turbulence. Because inertial waves are generated by winds at the surface, and internal tides are generated by strong tide-topography interactions, the analysis and justification in this paper support in principle the abyssal recipes Ⅱ:energetics of tidal and wind mixing by Munk Wunsch in 1998, in despite of the results of microstructure measurements for the turbulent kinetic energy dissipation rate and the diapycnal turbulent eddy diffusivity. 展开更多
关键词 internal waves inertial waves internal tides TURBULENCE oceanic interior mixing
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Distribution of Vertical Turbulent Mixing Parameter Caused by Internal Tidal Waves and Solitary Waves in the South Yellow Sea 被引量:1
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作者 SI Zongshang fan zhisong DU Ling 《Journal of Ocean University of China》 SCIE CAS 2012年第3期279-289,共11页
Many observations show that in the Yellow Sea internal tidal waves (ITWs) possess the remarkable characteristics of internal Kelvin wave, and in the South Yellow Sea (SYS) the nonlinear evolution of internal tidal wav... Many observations show that in the Yellow Sea internal tidal waves (ITWs) possess the remarkable characteristics of internal Kelvin wave, and in the South Yellow Sea (SYS) the nonlinear evolution of internal tidal waves is one of the mechanisms producing internal solitary waves (ISWs), which is different from the generation mechanism in the case where the semidiurnal tidal current flows over topographic drops. In this paper, the model of internal Kelvin wave with continuous stratification is given, and an elementary numerical study of nonlinear evolution of ITWs is made for the SYS, using the generalized KdV model (GKdV model for short) for a continuous stratified ocean, in which the different effects of background barotropic ebb and flood currents are considered. Moreover, the parameterization of vertical turbulent mixing caused by ITWs and ISWs in the SYS is studied, using a parameterization scheme which was applied to numerical experiments on the breaking of ISWs by Vlasenko and Hutter in 2002. It is found that the vertical turbulent mixing caused by internal waves is very strong within the upper layer with depth less than about 30m, and the vertical turbulent mixing caused by ISWs is stronger than that by ITWs. 展开更多
关键词 internal tidal wave internal solitary wave vertical turbulent mixing the South Yellow Sea
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电磁铆接初始温度对TB2铆钉绝热剪切变形的影响
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作者 姚钰杰 邓将华 +1 位作者 范治松 卢圳彬 《稀有金属》 EI CAS CSCD 北大核心 2024年第7期976-988,共13页
为提高电磁铆接接头质量,在210 V放电电压下分别对20℃和加热到100, 200和300℃的TB2铆钉进行电磁铆接工艺试验,并对铆钉镦头微观组织进行观察,分析绝热剪切带内微观组织演变及剪切带的形成机制。结果表明,随着初始温度的升高、铆钉的... 为提高电磁铆接接头质量,在210 V放电电压下分别对20℃和加热到100, 200和300℃的TB2铆钉进行电磁铆接工艺试验,并对铆钉镦头微观组织进行观察,分析绝热剪切带内微观组织演变及剪切带的形成机制。结果表明,随着初始温度的升高、铆钉的变形量增加,当温度达到300℃时,TB2铆钉最大变形量接近58%,相比于20℃时提高了20%。观察到绝热剪切变形过程中剪切带经历萌生、扩展到断裂3个阶段,初始温度的升高会延缓剪切带的出现,剪切带的宽度受温度和变形量两个因素的影响。从过渡区域到剪切带中心方向上的位错密度逐渐降低,拉长的大晶粒内存在位错胞,随着变形量的增加,大晶粒逐渐破碎成等轴纳米级晶粒,符合旋转动态再结晶晶粒形成的特征。 展开更多
关键词 TB2钛合金 电磁铆接 绝热剪切带 旋转动态再结晶
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6063铝/20钢管磁脉冲胀形连接工艺研究
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作者 范治松 李宁 +1 位作者 徐立凯 邓将华 《中国机械工程》 EI CAS 2024年第12期2185-2192,共8页
为探究带集磁器结构的异种金属管材磁脉冲胀形连接新方法,分析了集磁器条件下电磁力分布和铝管的胀形连接行为规律,通过连接接头拉脱实验,研究了主要工艺参数对磁脉冲胀形连接效果的影响。结果表明:集磁器显著提高了铝管胀形区电磁力,... 为探究带集磁器结构的异种金属管材磁脉冲胀形连接新方法,分析了集磁器条件下电磁力分布和铝管的胀形连接行为规律,通过连接接头拉脱实验,研究了主要工艺参数对磁脉冲胀形连接效果的影响。结果表明:集磁器显著提高了铝管胀形区电磁力,铝管与集磁器工作区的搭接长度对铝管胀形变形影响较大,当搭接长度为集磁器工作区长度的1.5倍时,获得了最大的有效接触面积;在外管仅发生弹性变形时,碰撞压力与放电电压呈正相关,接头抗拉脱载荷随放电电压增大而增大;在相同条件下,接头抗拉脱载荷随着径向间隙的增大呈现出先增大后减小的变化趋势,当放电电压为17 kV、径向间隙为4.0 mm、搭接长度为15 mm时,接头抗拉脱载荷最大,达到5021 N。 展开更多
关键词 磁脉冲胀形连接 集磁器 6063铝合金 拉脱载荷 数值模拟
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