Phytochemical investigations of the ethanolic extract of leaves of Clutia lanceolata(Family:Euphorbiaceae)resulted in the isolation of four compounds viz.3,4-dihydroxy-2-methylbenzoic acid(1),2,20-dihydroxy-1,10-binap...Phytochemical investigations of the ethanolic extract of leaves of Clutia lanceolata(Family:Euphorbiaceae)resulted in the isolation of four compounds viz.3,4-dihydroxy-2-methylbenzoic acid(1),2,20-dihydroxy-1,10-binaphthyl(2),1,3,8-trihydroxy-6-methylanthracene-9,10-dione(3)and 5-hydroxy-1,7-bis(4-hydroxy-3-methoxyphenyl)hepta-1,4,6-trien-3-one(4).Although all the isolated compounds were known but this was the first report from this plant source.Their structures were established on the basis of chemical and physical evidences viz.elemental analysis,FT-IR,1 H-NMR,13CNMR and mass spectral analysis.Structure of compound 2 and 4 was further authenticated by single-crystal X-ray analysis and density functional theory calculations.The isolated compounds(1–4)were screened for AChE enzyme inhibition assay in which compound 3 and 4 were found to be more potent AChE inhibitor.Molecular docking study of potent AChE inhibitor was performed to find the probable binding mode of the compounds into the active site of receptor.Moreover,the isolated compounds were also screened for in vivo cytotoxicity by brine shrimp lethality assay.展开更多
The pine sawyer beetle, Monochamus alternatus, is regarded as a notorious for- est pest in Asia, vectoring an invasive pathogenic nematode, Bursaphelenchus xylophilus, which is known to cause pine wilt disease. Howeve...The pine sawyer beetle, Monochamus alternatus, is regarded as a notorious for- est pest in Asia, vectoring an invasive pathogenic nematode, Bursaphelenchus xylophilus, which is known to cause pine wilt disease. However, little sequence information is available for this vector beetle. This hampered the research on its immune system. Based on the transcriptome of M. alternatus, we have identified and characterized 194 immunity-related genes in M. alternatus, and compared them with homologues molecules from other species known to exhibit immune responses against invading microbes. The lower number of puta- tive immunity-related genes in M. alternatus were attributed to fewer C-type lectin, serine protease （SP） and anti-microbial peptide （AMP） genes. Phylogenetic analysis revealed that M. alternatus had a unique recognition gene, galectin3, orthologues of which were not identified in Tribolium castaneum, Drosophila melanogastor, Anopheles gambiae and Apis mellifera. This suggested a lineage-specific gene evolution for coleopteran insects. Our study provides the comprehensive sequence resources of the immunity-related genes ofM. alternatus, presenting valuable information for better understanding of the molecular mechanism of innate immunity processes in M. alternatus against B. xylophilus.展开更多
Plants in their natural environment are constantly subjected to various abiotic and biotic stressors and,therefore,have developed several defense mechanisms to maintain fitness.Stress responses are intricate and requi...Plants in their natural environment are constantly subjected to various abiotic and biotic stressors and,therefore,have developed several defense mechanisms to maintain fitness.Stress responses are intricate and require various physiological,biochemical,and cellular changes in plants.The reaction mechanisms in plants subjected to drought,salinity,or heat stress alone have been explained in numerous studies.However,the field conditions are significantly different from the controlled laboratory conditions.In the field,crops or plants are simultaneously exposed to two or more abiotic and/or biotic stress conditions,such as a combination of salinity and heat,drought and cold,or any of the abiotic stresses combined with pathogen infection.Studies have shown that plants’reactions to combinations of more than two stress factors are distinct and cannot be explicitly deduced from their responses to different stresses when applied separately.Therefore,additional research is needed to understand the complete mechanism of plant responses to stress by analyzing data between single stress and multiple stress responses.This review aims to provide an overview of current research on plant responses to a combination of various stress conditions and their influence on the metabolic,transcriptional,and physiological characteristics of plants.展开更多
This study focused,for the first time,on the effect of ultrasonic features on the extraction efficiency of secondary metabolites in mustard seed cake(MSC).The nematostatic potential of sonicated seed cake was examined...This study focused,for the first time,on the effect of ultrasonic features on the extraction efficiency of secondary metabolites in mustard seed cake(MSC).The nematostatic potential of sonicated seed cake was examined against the second-stage juveniles(J2s)of root-knot nematode,Meloidogyne javanica.The results show that a 35 ppm(parts per million)concentration of a sonicated extract(SE)sample of MSC caused 65%J2s mortality at 18 h exposure period in vitro.It also significantly suppressed the root-knot index(RKI=0.94)in tomato roots.The lethal concentration values for SE were 51.76,29.79,and 13.34 ppm,respectively,at 6,12,and 18 h of the exposure period,and the lethal concentration values for the non-sonicated extract(NSE)sample were 116.95,76.38,and 55.59 ppm,respectively,at similar exposure time.Sinapine and gluconapin were identified as the major compounds in ultrasonic-assisted MSC.Because of the high extraction efficiency of metabolites in the SE,all treat‐ments of SE were shown to be antagonistic to J2s.Thus,this study of ultrasonication activity-based profiling of MSC may help generate target-based compounds at a scale relevant to the control of disease caused by nematodes in economic crops.展开更多
Monochamus alternatus, the main vector beetles of invasive pinewood nematode, has established a symbiotic relationship with a native ectotrophic fungal symbiont, Sporothrix sp. 1, in China. The immune response ofM. al...Monochamus alternatus, the main vector beetles of invasive pinewood nematode, has established a symbiotic relationship with a native ectotrophic fungal symbiont, Sporothrix sp. 1, in China. The immune response ofM. alternatus to S. sp. 1 in the coexistence of beetles and fungi is, however, unknown. Here, we report that immune responses ofM. alternatus pupae to infection caused by ectotrophic symbiotic fungus S. sp. 1 and entomopathogenic fungus Beauveria bassiana differ significantly. The S. sp. 1 did not kill the beetles while B. bassiana killed all upon injection. The transcriptome results showed that the numbers of differentially expressed genes in M. aIternatus infected with S. sp. 1 were 2-fold less than those infected with B. bassiana at 48 hours post infection. It was noticed that Toll and IMD pathways played a leading role in the beetle＇s immune system when infected by symbiotic fungus, but upon infection by entomopathogenic fimgus, only the Toll pathway gets triggered actively. Furthermore, the beetles could tolerate the infection of symbiotic fungi by retracing their Toll and IMD pathways at 48 h. This study provided a comprehensive sequence resource ofM. alternatus transcriptome for further study of the immune interactions between host and associated fungi.展开更多
文摘Phytochemical investigations of the ethanolic extract of leaves of Clutia lanceolata(Family:Euphorbiaceae)resulted in the isolation of four compounds viz.3,4-dihydroxy-2-methylbenzoic acid(1),2,20-dihydroxy-1,10-binaphthyl(2),1,3,8-trihydroxy-6-methylanthracene-9,10-dione(3)and 5-hydroxy-1,7-bis(4-hydroxy-3-methoxyphenyl)hepta-1,4,6-trien-3-one(4).Although all the isolated compounds were known but this was the first report from this plant source.Their structures were established on the basis of chemical and physical evidences viz.elemental analysis,FT-IR,1 H-NMR,13CNMR and mass spectral analysis.Structure of compound 2 and 4 was further authenticated by single-crystal X-ray analysis and density functional theory calculations.The isolated compounds(1–4)were screened for AChE enzyme inhibition assay in which compound 3 and 4 were found to be more potent AChE inhibitor.Molecular docking study of potent AChE inhibitor was performed to find the probable binding mode of the compounds into the active site of receptor.Moreover,the isolated compounds were also screened for in vivo cytotoxicity by brine shrimp lethality assay.
文摘The pine sawyer beetle, Monochamus alternatus, is regarded as a notorious for- est pest in Asia, vectoring an invasive pathogenic nematode, Bursaphelenchus xylophilus, which is known to cause pine wilt disease. However, little sequence information is available for this vector beetle. This hampered the research on its immune system. Based on the transcriptome of M. alternatus, we have identified and characterized 194 immunity-related genes in M. alternatus, and compared them with homologues molecules from other species known to exhibit immune responses against invading microbes. The lower number of puta- tive immunity-related genes in M. alternatus were attributed to fewer C-type lectin, serine protease （SP） and anti-microbial peptide （AMP） genes. Phylogenetic analysis revealed that M. alternatus had a unique recognition gene, galectin3, orthologues of which were not identified in Tribolium castaneum, Drosophila melanogastor, Anopheles gambiae and Apis mellifera. This suggested a lineage-specific gene evolution for coleopteran insects. Our study provides the comprehensive sequence resources of the immunity-related genes ofM. alternatus, presenting valuable information for better understanding of the molecular mechanism of innate immunity processes in M. alternatus against B. xylophilus.
基金the University Grants Commission(UGC-BSR Research Start-up-Grant No.F30-409/2018)the Deanship of Scientific Research,King Khalid University,Saudi Arabia(No.R.G.P.2/11/42)for their financial assistance。
文摘Plants in their natural environment are constantly subjected to various abiotic and biotic stressors and,therefore,have developed several defense mechanisms to maintain fitness.Stress responses are intricate and require various physiological,biochemical,and cellular changes in plants.The reaction mechanisms in plants subjected to drought,salinity,or heat stress alone have been explained in numerous studies.However,the field conditions are significantly different from the controlled laboratory conditions.In the field,crops or plants are simultaneously exposed to two or more abiotic and/or biotic stress conditions,such as a combination of salinity and heat,drought and cold,or any of the abiotic stresses combined with pathogen infection.Studies have shown that plants’reactions to combinations of more than two stress factors are distinct and cannot be explicitly deduced from their responses to different stresses when applied separately.Therefore,additional research is needed to understand the complete mechanism of plant responses to stress by analyzing data between single stress and multiple stress responses.This review aims to provide an overview of current research on plant responses to a combination of various stress conditions and their influence on the metabolic,transcriptional,and physiological characteristics of plants.
基金the University Grants Com‐mission(UGC-BSR Research Start-up-Grant:F30-409/2018),India.
文摘This study focused,for the first time,on the effect of ultrasonic features on the extraction efficiency of secondary metabolites in mustard seed cake(MSC).The nematostatic potential of sonicated seed cake was examined against the second-stage juveniles(J2s)of root-knot nematode,Meloidogyne javanica.The results show that a 35 ppm(parts per million)concentration of a sonicated extract(SE)sample of MSC caused 65%J2s mortality at 18 h exposure period in vitro.It also significantly suppressed the root-knot index(RKI=0.94)in tomato roots.The lethal concentration values for SE were 51.76,29.79,and 13.34 ppm,respectively,at 6,12,and 18 h of the exposure period,and the lethal concentration values for the non-sonicated extract(NSE)sample were 116.95,76.38,and 55.59 ppm,respectively,at similar exposure time.Sinapine and gluconapin were identified as the major compounds in ultrasonic-assisted MSC.Because of the high extraction efficiency of metabolites in the SE,all treat‐ments of SE were shown to be antagonistic to J2s.Thus,this study of ultrasonication activity-based profiling of MSC may help generate target-based compounds at a scale relevant to the control of disease caused by nematodes in economic crops.
基金supported by the Forestry Industry Research Special Funds for Public Welfare Project(201204501)National Key Plan for Scientific Research and Development of China(2016YFC1200604,2016YFD0500300)+2 种基金the High Technology Research and Development Program(HTRDP)of China(2014AA020529)National Natural Science Foundation of China(31572272,31370650,31402013,31221091,31672291,L1524009)the CAS Key Research Projects of the Frontier Science(QYZDBSSW-SMC014),and CAS(2015-SM-C-02)
文摘Monochamus alternatus, the main vector beetles of invasive pinewood nematode, has established a symbiotic relationship with a native ectotrophic fungal symbiont, Sporothrix sp. 1, in China. The immune response ofM. alternatus to S. sp. 1 in the coexistence of beetles and fungi is, however, unknown. Here, we report that immune responses ofM. alternatus pupae to infection caused by ectotrophic symbiotic fungus S. sp. 1 and entomopathogenic fungus Beauveria bassiana differ significantly. The S. sp. 1 did not kill the beetles while B. bassiana killed all upon injection. The transcriptome results showed that the numbers of differentially expressed genes in M. aIternatus infected with S. sp. 1 were 2-fold less than those infected with B. bassiana at 48 hours post infection. It was noticed that Toll and IMD pathways played a leading role in the beetle＇s immune system when infected by symbiotic fungus, but upon infection by entomopathogenic fimgus, only the Toll pathway gets triggered actively. Furthermore, the beetles could tolerate the infection of symbiotic fungi by retracing their Toll and IMD pathways at 48 h. This study provided a comprehensive sequence resource ofM. alternatus transcriptome for further study of the immune interactions between host and associated fungi.
