目的 通过对抖音平台数据挖掘,基于详尽可能模型(Elaboration Likelihood Model, ELM)分析省级疾病预防控制中心(Center for Disease Control and Prevention, CDC)短视频影响因素及传播效果,以期为CDC借助短视频开展服务提供参考。方法...目的 通过对抖音平台数据挖掘,基于详尽可能模型(Elaboration Likelihood Model, ELM)分析省级疾病预防控制中心(Center for Disease Control and Prevention, CDC)短视频影响因素及传播效果,以期为CDC借助短视频开展服务提供参考。方法 以15个省级CDC的2021年全部短视频数据为调查对象,通过Python程序抓取抖音账号的基本数据、描述传播特征、基于ELM模型设置10个维度,采用普通最小二乘法(Ordinary Least Squares, OLS)回归分析进行假设检验。结果 15个省级疾控中心抖音号共纳入1277条视频,其在粉丝数(0.06万~13万)、点赞数(0.09万~20.04万)、转发数(0.01万~2.05万)、评论数(0~0.86万)、抖音传播力指数(Douyin Communication Index, DCI)(595~1016.28)均存在差距;以中心路径和边缘路径作为ELM模型评价指标,在中心路径中,知识类、新闻类对传播效果有显著作用,其他类及低质量视频有显著负向影响;从边缘路径看,现场直播的方式、时长为121~180 s、“语音+字幕”的播报形式、庄严的背景音乐、感叹句式标题的短视频对传播效果具有正向影响,不带“#”话题的视频内容有负向影响;以及可能存在其他调节变量。结论 CDC应多以传播疾病预防知识、传递新闻信息为主;更加注重内容质量的输出与用户需求的互动;参考现场直播拍摄方式、“语音+字幕”、庄严的背景音乐、感叹句式标题,控制时长,提升传播效果和影响力。展开更多
The canonical transient receptor potential channel(TRPC)proteins form Ca^(2+)-permeable cation channels that are involved in various heart diseases.However,the roles of specific TRPC proteins in myocardial ischemia/re...The canonical transient receptor potential channel(TRPC)proteins form Ca^(2+)-permeable cation channels that are involved in various heart diseases.However,the roles of specific TRPC proteins in myocardial ischemia/reperfusion(I/R)injury remain poorly understood.We observed that TRPC1 and TRPC6 were highly expressed in the area at risk(AAR)in a coronary artery ligation induced I/R model.Trpc1/mice exhibited improved cardiac function,lower serum Troponin T and serum creatine kinase level,smaller infarct volume,less fibrotic scars,and fewer apoptotic cells after myocardial-I/R than wild-type or Trpc6/mice.Cardiomyocyte-specific knockdown of Trpc1 using adeno-associated virus 9 mitigated myocardial I/R injury.Furthermore,Trpc1 deficiency protected adult mouse ventricular myocytes(AMVMs)and HL-1 cells from death during hypoxia/reoxygenation(H/R)injury.RNA-sequencing-based transcriptome analysis revealed differential expression of genes related to reactive oxygen species(ROS)generation in Trpc1/cardiomyocytes.Among these genes,oxoglutarate dehydrogenase-like(Ogdhl)was markedly downregulated.Moreover,Trpc1 deficiency impaired the calcineurin(CaN)/nuclear factorkappa B(NF-kB)signaling pathway in AMVMs.Suppression of this pathway inhibited Ogdhl upregulation and ROS generation in HL-1 cells under H/R conditions.Chromatin immunoprecipitation assays confirmed NF-kB binding to the Ogdhl promoter.The cardioprotective effect of Trpc1 deficiency was canceled out by overexpression of NF-kB and Ogdhl in cardiomyocytes.In conclusion,our findings reveal that TRPC1 is upregulated in the AAR following myocardial I/R,leading to increased Ca^(2+) influx into associated cardiomyocytes.Subsequently,this upregulates Ogdhl expression through the CaN/NF-kB signaling pathway,ultimately exacerbating ROS production and aggravating myocardial I/R injury.展开更多
文摘目的探讨输尿管软镜碎石取石术(flexible ureteroscopic lithotripsy,fURL)术前预置双J管对肾结石患者疗效的影响。方法回顾性分析2021年9月至2022年3月安徽医科大学第一附属医院收治的180例行fURL肾结石患者的临床资料。对照组(n=100)术前未预置输尿管支架,研究组(n=80)术前2周预置双J管。比较两组围手术期临床指标、视觉模拟评分法(visual analogue scale,VAS)、威斯康辛生活质量问卷(the Wisconsin Stone Quality of Life questionnaire,WISQOL)评分。结果对照组术后出现输尿管黏膜损伤3例,持续高热2例,研究组无并发症发生,对照组并发症发生率高于研究组,差异有统计学意义(P<0.05);对照组术前尿白细胞计数、初始尿培养阳性率、术前膀胱过度活动症(overactive bladder,OAB)发生率、一次输尿管通道鞘(ureteral access sheath,UAS)置入率均低于研究组,差异有统计学意义(P<0.05)。术后,对照组VAS评分低于研究组,WISQOL评分高于研究组,差异均有统计学意义(P<0.05);两组精力、睡眠、工作和家庭、对于出行和亲密行为的关注评分差值比较,差异无统计学意义(P>0.05);对照组营养和药物治疗、症状、总体情绪评分差值均高于研究组,差异有统计学意义(P<0.05)。结论术前预置双J管能够提高fURL中一次UAS置入成功率,但会降低患者生活质量,增加OAB和尿路感染发生率,且需承担额外的术前置双J管手术风险和费用。
文摘目的 通过对抖音平台数据挖掘,基于详尽可能模型(Elaboration Likelihood Model, ELM)分析省级疾病预防控制中心(Center for Disease Control and Prevention, CDC)短视频影响因素及传播效果,以期为CDC借助短视频开展服务提供参考。方法 以15个省级CDC的2021年全部短视频数据为调查对象,通过Python程序抓取抖音账号的基本数据、描述传播特征、基于ELM模型设置10个维度,采用普通最小二乘法(Ordinary Least Squares, OLS)回归分析进行假设检验。结果 15个省级疾控中心抖音号共纳入1277条视频,其在粉丝数(0.06万~13万)、点赞数(0.09万~20.04万)、转发数(0.01万~2.05万)、评论数(0~0.86万)、抖音传播力指数(Douyin Communication Index, DCI)(595~1016.28)均存在差距;以中心路径和边缘路径作为ELM模型评价指标,在中心路径中,知识类、新闻类对传播效果有显著作用,其他类及低质量视频有显著负向影响;从边缘路径看,现场直播的方式、时长为121~180 s、“语音+字幕”的播报形式、庄严的背景音乐、感叹句式标题的短视频对传播效果具有正向影响,不带“#”话题的视频内容有负向影响;以及可能存在其他调节变量。结论 CDC应多以传播疾病预防知识、传递新闻信息为主;更加注重内容质量的输出与用户需求的互动;参考现场直播拍摄方式、“语音+字幕”、庄严的背景音乐、感叹句式标题,控制时长,提升传播效果和影响力。
基金supported by the National Natural Science Foundation of China(Grant Nos.:81970245,82270357,and 81770432)the Scientific Research Project of Shaanxi Administration of Traditional Chinese Medicine,China(Grant Nos.:2021-04-ZZ-001,2021-QYPT-003,and 2022-SLRH-YQ-004)+1 种基金the Project of Science and Technology Department of Shaanxi Province in China(Project No.:2022YWZX-PG-01)the Natural Science Basic Research Program of Shaanxi Province in China(Grant No.:2023-JC-JQ-61).
文摘The canonical transient receptor potential channel(TRPC)proteins form Ca^(2+)-permeable cation channels that are involved in various heart diseases.However,the roles of specific TRPC proteins in myocardial ischemia/reperfusion(I/R)injury remain poorly understood.We observed that TRPC1 and TRPC6 were highly expressed in the area at risk(AAR)in a coronary artery ligation induced I/R model.Trpc1/mice exhibited improved cardiac function,lower serum Troponin T and serum creatine kinase level,smaller infarct volume,less fibrotic scars,and fewer apoptotic cells after myocardial-I/R than wild-type or Trpc6/mice.Cardiomyocyte-specific knockdown of Trpc1 using adeno-associated virus 9 mitigated myocardial I/R injury.Furthermore,Trpc1 deficiency protected adult mouse ventricular myocytes(AMVMs)and HL-1 cells from death during hypoxia/reoxygenation(H/R)injury.RNA-sequencing-based transcriptome analysis revealed differential expression of genes related to reactive oxygen species(ROS)generation in Trpc1/cardiomyocytes.Among these genes,oxoglutarate dehydrogenase-like(Ogdhl)was markedly downregulated.Moreover,Trpc1 deficiency impaired the calcineurin(CaN)/nuclear factorkappa B(NF-kB)signaling pathway in AMVMs.Suppression of this pathway inhibited Ogdhl upregulation and ROS generation in HL-1 cells under H/R conditions.Chromatin immunoprecipitation assays confirmed NF-kB binding to the Ogdhl promoter.The cardioprotective effect of Trpc1 deficiency was canceled out by overexpression of NF-kB and Ogdhl in cardiomyocytes.In conclusion,our findings reveal that TRPC1 is upregulated in the AAR following myocardial I/R,leading to increased Ca^(2+) influx into associated cardiomyocytes.Subsequently,this upregulates Ogdhl expression through the CaN/NF-kB signaling pathway,ultimately exacerbating ROS production and aggravating myocardial I/R injury.
