Flowering phenology of plants,which is important for reproductive growth,has been shown to be influenced by climate change.Understanding how flowering phenology responds to climate change and exploring the variation o...Flowering phenology of plants,which is important for reproductive growth,has been shown to be influenced by climate change.Understanding how flowering phenology responds to climate change and exploring the variation of this response across plant groups can help predict structural and functional changes in plant communities in response to ongoing climate change.Here,we used long-term collections of 33 flowering plant species from the Gongga Mountains(Mt.Gongga hereafter),a biodiversity hotspot,to investigate how plant flowering phenology changed over the past 70 years in response to climate change.We found that mean flowering times in Mt.Gongga were delayed in all vegetation types and elevations over the last 70 years.Furthermore,flowering time was delayed more in lowlands than at high elevations.Interestingly,we observed that spring-flowering plants show earlier flowering times whereas summer/autumn plants show delayed flowering times.Non-synchronous flowering phenology across species was mainly driven by changes in temperature and precipitation.We also found that the flowering phenology of 78.8%plant species was delayed in response to warming temperatures.Our findings also indicate that the magnitude and direction of variation in plant flowering times vary significantly among species along elevation gradients.Shifts in flowering time might cause trophic mismatches with co-occurring and related species,affecting both forest ecosystem structure and function.展开更多
Global climate change can affect tree growth and carbon sink function by influencing plant carbohydrate synthesis and utilization,while elevation can be used as an ideal setting under natural conditions to simulate cl...Global climate change can affect tree growth and carbon sink function by influencing plant carbohydrate synthesis and utilization,while elevation can be used as an ideal setting under natural conditions to simulate climate change effects.The effect of elevation on tree growth may depend on organ type.However,the allocation patterns of nonstructural and structural carbohydrates(NSCs and SCs,respectively)in different tree organs and their response to elevation remain unclear.We selected four dominant tree species,Schima superba,Castanopsis eyrei,Castanopsis fargesii and Michelia maudiae,along an elevation gradient from 609 to 1,207 m in subtropical evergreen broad-leaved forests and analyzed leaf,trunk,and fine root NSCs,carbon(C),nitrogen(N)and phosphorus(P)concentrations and the relative abundance of SCs.Leaf NSCs increased initially and then decreased,and trunk NSCs increased with increasing elevation.However,root NSCs decreased with increasing elevation.The relative abundance of SCs in leaves and trunks decreased,while the relative abundance of root SCs increased with increasing elevation.No significant correlations between SCs and NSCs in leaves were detected,while there were negative correlations between SCs and NSCs in trunks,roots,and all organs.Hierarchical partitioning analysis indicated that plant C/N and C/P were the main predictors of changes in SCs and NSCs.Our results suggest that tree organs have divergent responses to elevation and that increasing elevation will inhibit the aboveground part growth and enhance the root growth of trees.A tradeoff between the C distribution used for growth and storage was confirmed along the elevation gradient,which is mainly manifested in the"sink"organs of NSCs.Our results provide insight into tree growth in the context of global climate change scenarios in subtropical forest ecosystems.展开更多
The liquid-liquid extraction method using reverse micelles can simultaneously extract lipid and protein of oilseeds,which have become increasingly popular in recent years.However,there are few studies on mass transfer...The liquid-liquid extraction method using reverse micelles can simultaneously extract lipid and protein of oilseeds,which have become increasingly popular in recent years.However,there are few studies on mass transfer processes and models,which are helpful to better control the extraction process of oils and proteins.In this paper,mass transfer process of peanut protein extracted by bis(2-ethylhexyl)sodium sulfosuccinate(AOT)/isooctane reverse micelles was investigated.The effects of stirring speed(0,70,140,and 210 r/min),temperature of extraction(30,35,40,45,and 50℃),peanut flour particle size(0.355,0.450,0.600,and 0.900 mm)and solidliquid ratio(0.010,0.0125,0.015,0.0175,and 0.020 g/mL)on extraction rate were examined.The results showed that extraction rate increased with temperature rising,particle size reduction as well as solid-liquid ratio increase respectively,while little effect of stirring speed(P>0.05)was observed.The apparent activation energy of extraction process was calculated as 10.02 kJ/mol and Arrhenius constant(A)was 1.91 by Arrhenius equation.There was a linear relationship between reaction rate constant and the square of the inverse of initial particle radius(1/r_(0)^(2))(P<0.05).This phenomenon and this shrinking core model were anastomosed.In brief,the extraction process was controlled by the diffusion of protein from the virgin zone interface of particle through the reacted zone and it was in line with the first order reaction.Mass transfer kinetics of peanut protein extracted by reverse micelles was established and it was verified by experimental results.The results provide an important theoretical guidance for industrial production of peanut protein separation and purification.展开更多
Tree competitiveness generally depends on trait plasticity in response to environmental change.The effects of nitrogen(N)and phosphorus(P)on leaf trait variability by species is poorly understood,especially in China’...Tree competitiveness generally depends on trait plasticity in response to environmental change.The effects of nitrogen(N)and phosphorus(P)on leaf trait variability by species is poorly understood,especially in China’s subtropical forests.This study examined the seedling leaf traits and net primary productivity of all trees>5 cm DBH of two dominant species,Schima superba and Castanopsis carlesii,in an evergreen broadleaved forest fertilized with nitrogen(+N),phosphorus(+P),and nitrogen plus phosphorus(N+P).The effect of N on seedling leaf traits was stronger than P,while fertilization in general was species dependent.Leaf mass per unit area decreased with N for S.superba seedlings but not for C.carlesii.Leaf N,P,and N/P ratios changed with N addition for both species.All four N fractions of carboxylation,bioenergetics,cell wall,and other N metabolites in C.carlesii leaves responded significantly to fertilization,while only the cell wall in S.superb a leaves responded.Other leaf functional traits,including light-saturated photosynthetic rates,water,N,and P use efficiencies,chlorophyll and non structural carbohydrate contents increased with N addition in S.superb a and by P addition in C.carlesii.Canopy closure at the stand-level increased due to N.Litter biomass and relative growth rate of S.superb a was not affected by any treatments,while both for C.carlesii significantly decreased with N+P addition.Collectively,nutrient limitation may vary at a small scale among species in a subtropical forest based on their responses of seedling traits and net primary productivity to fertilization.Seedling traits are not correlated with the net primary productivity of larger trees except for N fractions,because low light conditions induced by fertilization reduces the proportion of N allocated to photosynthesis in seedlings.In addition,acclimation differences of tree species may increase the uncertainty of community succession.展开更多
Background:The replanting of broadleaved trees in pure coniferous plantations is widely implemented,as mixed plantations are generally more stable and functional.However,the effect of interspecific interactions betwee...Background:The replanting of broadleaved trees in pure coniferous plantations is widely implemented,as mixed plantations are generally more stable and functional.However,the effect of interspecific interactions between broadleaved and coniferous trees on internal nutrient cycles of conifers remains unclear.Methods:We selected pure coniferous plantations of a native(Pinus massoniana)and an exotic(P.elliottii)pine species and their corresponding mixed plantations with broadleaved trees(Schima superba)in subtropical China,and measured the nitrogen(N)and phosphorus(P)contents in the rhizosphere soils,fine roots,twigs,needles and needle litter of pines.We calculated the root capture,needle resorption and translocation of N and P by pines to determine the mobility of nutrients in trees.Results:Although the N and P in the rhizosphere soils increased due to the replanting of broadleaved trees,the N and P contents in the aboveground tissues of the two pine species did not increase in mixed plantations.Mixed planting had a negative effect on the N and P capture of native pine and a positive effect on that of exotic pine.The N and P resorption efficiencies increased in native pine but were unchanged in exotic pine after the replanting of S.superba.Native pine preferentially employed an aboveground nutrient resorption strategy,whereas exotic pine tended to adopt a belowground nutrient capture strategy after replanting.Translocation of N and P in trees was detected,which reflected the trade-offs between root nutrient capture and needle nutrient resorption.Conclusions:The effect of mixed planting varied between the species of native and exotic pines,and the internal nutrient cycles of both pine species might be dominated by interspecific interaction effects on nutrients rather than soil nutrients.Our study highlights the importance of selecting suitable broadleaved species for replanting in coniferous plantations.展开更多
Nitrogen (N) and phosphorus (P) additions can affect soil microbial carbon (C) accumulation. However, the mechanisms that drive the changes in residual microbial C that occur after N and P additions have not bee...Nitrogen (N) and phosphorus (P) additions can affect soil microbial carbon (C) accumulation. However, the mechanisms that drive the changes in residual microbial C that occur after N and P additions have not been well-defined for Chinese fir plantations in subtropical China. We set up six different treatments, viz. a control (CK), two N treatments (NI: 50kgha-1 a-1; N2: 100 kg ha-1 a-1), one P treatment (P: 50 kg ha-1 a-1), and two combined N and P treatments (NIP: 50kgha-1a-1 of N +50kgha-1a-1 of P; N2P:100 kg ha-1 a-1 of N + 50 kg ha-1 a-1 of P). We then investigated the influences of N and P additions on residual microbial C. The results showed that soil pH and microbial biomass decreased after N additions, while microbial biomass increased after P additions. Soil organic carbon (SOC) and residual microbial C contents increased in the N and P treatments but not in the control. Residual microbial C accumulation varied according to treatment and declined in the order: N2P 〉 N1P 〉 N2 〉 N1 〉 P 〉 CK. Residual microbial C contents were positively correlated with available N, P, and SOC contents, but were negatively correlated with soil pH. The ratio of residual fungal C to residual bacterial C increased under P additions, but declined under combined N1P additions. The ratio of residual microbial C to SOC increased from 11 to 14% under the N1P and N2P treatments, respectively. Our results suggest that the concentrations of residual microbial C and the stability of SOC would increase under combined applications of N and P fertilizers in subtropical Chinese fir plantation soils.展开更多
High-fidelity two-qubit gates are essential for the realization of large-scale quantum computation and simulation.Tunable coupler design is used to reduce the problem of parasitic coupling and frequency crowding in ma...High-fidelity two-qubit gates are essential for the realization of large-scale quantum computation and simulation.Tunable coupler design is used to reduce the problem of parasitic coupling and frequency crowding in manyqubit systems and thus thought to be advantageous. Here we design an extensible 5-qubit system in which center transmon qubit can couple to every four near-neighboring qubits via a capacitive tunable coupler and experimentally demonstrate high-fidelity controlled-phase(CZ) gate by manipulating central qubit and one nearneighboring qubit. Speckle purity benchmarking and cross entropy benchmarking are used to assess the purity fidelity and the fidelity of the CZ gate. The average purity fidelity of the CZ gate is 99.69±0.04% and the average fidelity of the CZ gate is 99.65±0.04%, which means that the control error is about 0.04%. Our work is helpful for resolving many challenges in implementation of large-scale quantum systems.展开更多
Foodborne pathogen poses a threat to the food industries as many outbreaks have been found to be associated with biofilm formation.The formation of biofilm is a self-protection growth pattern of bacteria,which increas...Foodborne pathogen poses a threat to the food industries as many outbreaks have been found to be associated with biofilm formation.The formation of biofilm is a self-protection growth pattern of bacteria,which increases post-processing contamination and risk to public health.It is difficult to eliminate the biofilm in the food industries,since the biofilm cells have a barrier preventing or lessening the contact with environmental stresses,antimicrobial agents and the host immune system.Bacterial biofilm formation is a complex process,including initial attachment stage,irreversible attachment stage,biofilm development stage,biofilm maturation stage,and biofilm dispersion stage.The genetic mechanism,substratum and bacterial cell surface properties involve in the biofilm formation.The biofilm inhibition methods studied are physical treatment,chemical and biochemical treatment.The potential green and safe biochemical method attracts more attention,especially,the novel strategies using the safe biochemical agents(essential oils,enzymes,biosurfactants,others)constantly emerged.The review emphasized on effective strategies for inhibiting biofilm formation in different stages(initial irreversible attachment,formation,and maturation)by use of biochemical agents,aiming to provide new insight into biofilm control in food industry thus improving food quality and safety.展开更多
The development of high-fidelity two-qubit quantum gates is essential for digital quantum computing.Here,we propose and realize an all-microwave parametric controlled-Z(CZ)gates by coupling strength modulation in a su...The development of high-fidelity two-qubit quantum gates is essential for digital quantum computing.Here,we propose and realize an all-microwave parametric controlled-Z(CZ)gates by coupling strength modulation in a superconducting Transmon qubit system with tunable couplers.After optimizing the design of the tunable coupler together with the control pulse numerically,we experimentally realized a 100 ns CZ gate with high fidelity of 99.38%±0.34%and the control error being 0.1%.We note that our CZ gates are not affected by pulse distortion and do not need pulse correction,providing a solution for the real-time pulse generation in a dynamic quantum feedback circuit.With the expectation of utilizing our all-microwave control scheme to reduce the number of control lines through frequency multiplexing in the future,our scheme draws a blueprint for the high-integrable quantum hardware design.展开更多
Sericin silkworm is a kind of gene mutant silkworm that can only synthesis and secrete sericin protein instead of fibroin protein. In this study, according to the knowledge of physiology and heredity, the reasons of t...Sericin silkworm is a kind of gene mutant silkworm that can only synthesis and secrete sericin protein instead of fibroin protein. In this study, according to the knowledge of physiology and heredity, the reasons of this special phenomenon for the sericin silkworm were analyzed in order to explore the utilization clues in the production of natural sericin and product development.展开更多
Atmospheric vapor pressure deficit(VPD)increases with climate warming and may limit plant growth.However,gross primary production(GPP)responses to VPD remain a mystery,offering a significant source of uncertainty in t...Atmospheric vapor pressure deficit(VPD)increases with climate warming and may limit plant growth.However,gross primary production(GPP)responses to VPD remain a mystery,offering a significant source of uncertainty in the estimation of global terrestrial ecosystems carbon dynamics.In this study,in-situ measurements,satellite-derived data,and Earth System Models(ESMs)simulations were analysed to show that the GPP of most ecosystems has a similar threshold in response to VPD:first increasing and then declining.When VPD exceeds these thresholds,atmospheric drought stress reduces soil moisture and stomatal conductance,thereby decreasing the productivity of terrestrial ecosystems.Current ESMs underscore CO_(2) fertilization effects but predict significant GPP decline in low-latitude ecosystems when VPD exceeds the thresholds.These results emphasize the impacts of climate warming on VPD and propose limitations to future ecosystems productivity caused by increased atmospheric water demand.Incorporating VPD,soil moisture,and canopy conductance interactions into ESMs enhances the prediction of terrestrial ecosystem responses to climate change.展开更多
To ensure a long-term quantum computational advantage,the quantum hardware should be upgraded to withstand the competition of continuously improved classical algorithms and hardwares.Here,we demonstrate a superconduct...To ensure a long-term quantum computational advantage,the quantum hardware should be upgraded to withstand the competition of continuously improved classical algorithms and hardwares.Here,we demonstrate a superconducting quantum computing systems Zuchongzhi 2.1,which has 66 qubits in a two-dimensional array in a tunable coupler architecture.The readout fidelity of Zuchongzhi 2.1 is considerably improved to an average of 97.74%.The more powerful quantum processor enables us to achieve larger-scale random quantum circuit sampling,with a system scale of up to 60 qubits and 24 cycles,and fidelity of FXEB=(3·66±0·345)×10^(-4).The achieved sampling task is about 6 orders of magnitude more difficult than that of Sycamore[Nature 574,505(2019)]in the classic simulation,and 3 orders of magnitude more difficult than the sampling task on Zuchongzhi 2.0[arXiv:2106.14734(2021)].The time consumption of classically simulating random circuit sampling experiment using state-of-the-art classical algorithm and supercomputer is extended to tens of thousands of years(about 4·8×104years),while Zuchongzhi 2.1 only takes about 4.2 h,thereby significantly enhancing the quantum computational advantage.展开更多
Pseudomonas spp.and Enterobacteriaceae are dominant spoilage bacteria in chicken during cold storage(0°C-4°C).In this study,high resolution spectra in the range of 900-1700 nm were acquired and preprocessed ...Pseudomonas spp.and Enterobacteriaceae are dominant spoilage bacteria in chicken during cold storage(0°C-4°C).In this study,high resolution spectra in the range of 900-1700 nm were acquired and preprocessed using Savitzky-Golay convolution smoothing(SGCS),standard normal variate(SNV)and multiplicative scatter correction(MSC),respectively,and then mined using partial least squares(PLS)algorithm to relate to the total counts of Pseudomonas spp.and Enterobacteriaceae(PEC)of fresh chicken breasts to predict PEC rapidly.The results showed that with full 900-1700 nm range wavelength,MSC-PLS model built with MSC spectra performed better than PLS models with other spectra(RAW-PLS,SGCS-PLS,SNV-PLS),with correlation coefficient(RP)of 0.954,root mean square error of prediction(RMSEP)of 0.396 log10 CFU/g and residual predictive deviation(RPD)of 3.33 in prediction set.Based on the 12 optimal wavelengths(902.2 nm,905.5 nm,923.6 nm,938.4 nm,946.7 nm,1025.7 nm,1124.4 nm,1211.6 nm,1269.2 nm,1653.7 nm,1691.8 nm and 1693.4 nm)selected from MSC spectra by successive projections algorithm(SPA),SPA-MSC-PLS model had RP of 0.954,RMSEP of 0.397 log10 CFU/g and RPD of 3.32,similar to MSC-PLS model.The overall study indicated that NIR spectra combined with PLS algorithm could be used to detect the PEC of chicken flesh in a rapid and non-destructive way.展开更多
Total viable count(TVC)is often used as an important indicator for chicken freshness evaluation.In this study,112 fresh chicken flesh samples were acquired after slaughtered and their hyperspectral images were collect...Total viable count(TVC)is often used as an important indicator for chicken freshness evaluation.In this study,112 fresh chicken flesh samples were acquired after slaughtered and their hyperspectral images were collected in the LW-NIR(900-1700 nm)range.The full LW-NIR spectra(486 wavebands)within the images were extracted and applied to related to reference TVC values measured in different storage periods,using partial least squares regression(PLSR)algorithm,resulting in high correlation coefficients(R)and low root mean square errors(RMSE),for either raw spectra or pretreatment spectra.By using regression coefficients(RC)method,20,18,17 and 20 optimal wavebands were respectively selected from raw spectra,baseline correction(BC)spectra,Savitzky-Golay convolution smoothing(SGCS)spectra and standard normal variate(SNV)spectra and applied for the optimization of original full waveband PLSR model.By comparison,RC-PLSR model based on the SGCS spectra showed a better performance in TVC prediction with RC of 0.98 and RMSEC of 0.35 log10 CFU/g in calibration set,and RP of 0.98 and RMSEP of 0.44 log10 CFU/g in prediction set.At last,by transferring the best RC-PLSR model,the dynamic TVC change during the storage was visualized by color maps to indicate the TVC spoilage degree.The overall study revealed that LW-NIR hyperspectral imaging combined with PLSR could be used to predict the freshness of chicken flesh.展开更多
Accurate and rapid determination of nitrite contents is an important step for guaranteeing sausage quality.This study attempted to mine hyperspectral data in the range of 900-1700 nm for non-destructive and rapid pred...Accurate and rapid determination of nitrite contents is an important step for guaranteeing sausage quality.This study attempted to mine hyperspectral data in the range of 900-1700 nm for non-destructive and rapid prediction of nitrite contents in sausages.The average spectra of 156 samples were collected to relate to the measured nitrite values by partial least squares(PLS)regression.Optimal wavelengths were respectively selected by successive projections algorithm(SPA)and regression coefficients(RC)to simplify the PLS model.The results indicated that PLS model established with 15 optimal wavelengths(900.5 nm,907.1 nm,908.8 nm,912.1 nm,915.4 nm,920.3 nm,922.0 nm,941.7 nm,979.6 nm,1083.2 nm,1213.2 nm,1353.0 nm,1460.2 nm,1595.6 nm and 1699.9 nm)selected by SPA had better performance with r C,r CV,r P of 0.92,0.89,0.89 and RMSEC,RMSECV,RMSEP of 0.41 mg/kg,0.89 mg/kg,0.49 mg/kg,respectively,for calibration set,cross-validation and prediction set.It was concluded that hyperspectral data could be mined by PLS&SPA for realizing the rapid evaluation of nitrite content in ham sausages.展开更多
Increasing atmospheric CO2 concentration impacts the terrestrial carbon(C) cycle by affecting plant photosynthesis, the flow of photosynthetically fixed C belowground, and soil C pool turnover. For managed agroecosyst...Increasing atmospheric CO2 concentration impacts the terrestrial carbon(C) cycle by affecting plant photosynthesis, the flow of photosynthetically fixed C belowground, and soil C pool turnover. For managed agroecosystems, how and to what extent the interactions between elevated CO2 and N fertilization levels influence the accumulation of photosynthesized C in crops and the incorporation of photosynthesized C into arable soil are in urgent need of exploration.We conducted an experiment simulating elevated CO2 with spring wheat(Triticum aestivum L.) planted in growth chambers.13C-enriched CO2 with an identical 13C abundance was continuously supplied at ambient and elevated CO2 concentrations(350 and 600 μmol mol-1, respectively) until wheat harvest.Three levels of N fertilizer application(equivalent to 80, 120, and 180 kg N ha-1 soil) were supplied for wheat growth at both CO2 concentrations. During the continuous 62-d 13CO2 labeling period, elevated CO2 and increased N fertilizer application increased photosynthesized C accumulation in wheat by 14%–24% and 11%–20%, respectively, as indicated by increased biomass production, whereas the C/N ratio in the roots increased under elevated CO2 but declined with increasing N fertilizer application levels. Wheat root deposition induced 1%–2.5% renewal of soil C after 62 d of 13CO2 labeling. Compared to ambient CO2, elevated CO2 increased the amount of photosynthesized C incorporated into soil by 20%–44%. However, higher application rates of N fertilizer reduced the net input of root-derived C in soil by approximately 8% under elevated CO2. For the wheat-soil system, elevated CO2 and increased N fertilizer application levels synergistically increased the amount of photosynthesized C. The pivotal role of plants in photosynthesized C accumulation under elevated CO2 was thereby enhanced in the short term by the increased N application. Therefore, robust N management could mediate C cycling and sequestration by influencing the interactions between plants and soil in agroecosystems under elevated CO2.展开更多
Litter decomposition impacts carbon(C)and nutrient cycling.Nitrogen(N)and phosphorus(P)addition as well as litter age impact litter decomposition.Effects of nutrient addition and litter age on litter decomposition may...Litter decomposition impacts carbon(C)and nutrient cycling.Nitrogen(N)and phosphorus(P)addition as well as litter age impact litter decomposition.Effects of nutrient addition and litter age on litter decomposition may impact emissions of soil nitrous oxide(N_(2)O),which is an important greenhouse gas.However,no study has examined the effects of interactions between litter age and nutrient addition on soil N_(2)O emissions,and explored the underlining mechanisms simultaneously,thus limiting our evaluation of litter decomposition effects on N_(2)O emissions.Litter with different age was collected from Cunninghamia lanceolata plantations experienced N and P addition treatments to examine the effects of nutrient addition and litter age on N_(2)O emissions by incubation study.Litter age generally increased N_(2)O emissions via a decrease in litter C:N ratio.While P addition decreased N_(2)O emissions,N addition increased them mainly by positive effects on soil enzymes as indicated by microbial functional genes associated with N_(2)O production and negative effects on litter C:N ratio.Litter age and nutrient addition interacted to impact soil N_(2)O emissions.In future forest management,both nutrient addition and litter age should be considered in evaluation of management effects on N_(2)O emissions,especially thinning or selectively cutting involving litter input with different age.展开更多
This study insightfully analyzed the relationship between chemical composition change,volatile flavor compounds(VFCs)and microbial structural distribution of Chinese-style(CFS)and Salami fermented sausages(WFS)during ...This study insightfully analyzed the relationship between chemical composition change,volatile flavor compounds(VFCs)and microbial structural distribution of Chinese-style(CFS)and Salami fermented sausages(WFS)during their fermentation processes(FP).The results showed pH,acid and peroxide values of CFS were higher than WFS during whole FP(38 d).At the end of fermentation(38 days),the pH value of CFS and WFS were 5.70±0.01 and 5.43±0.01,respectively.The acid value was 9.89±0.38 and 6.29±0.28 mg/g,respectively.The peroxide value was 0.21±0.01 and 0.14±0.01 g/100g,respectively.During 0-13 d,moisture contents of CFS were higher than WFS,while opposite in 14-38 d.Nitrite contents of WFS were higher than CFS.The total free-amino acid contents of CFS were significantly lower than WFS(p<0.05).The six groups of main VFCs in the two sausages were aldehydes,acids,alcohols,esters,ketones and hydrocarbons,respectively,and the contents of ketone in CFS were higher than WFS.However,the bacterial abundance in CFS increased gradually,while that in WFS’s increased firstly and then decreased.The abundances and diversities of fungi in WFS were higher than CFS.In brief,microbial structural distribution formed an obvious impact on the chemical composition quality of fermented sausages,the above results provided data support for the production improvement of Chinese-style and Salami fermented sausages.展开更多
基金supported by Jiangxi Provincial Department of Education Science and Technology Research Project(GJJ2200433)the Natural Science Foundation of Jiangxi,China(#20224BAB213033)+2 种基金the National Key Research and Development Program of China(#2018YFA0606104)National Natural Science Foundation of China(#32125026,#31988102)the Strategic Priority Research Program of Chinese Academy of Sciences(#XDB31000000).
文摘Flowering phenology of plants,which is important for reproductive growth,has been shown to be influenced by climate change.Understanding how flowering phenology responds to climate change and exploring the variation of this response across plant groups can help predict structural and functional changes in plant communities in response to ongoing climate change.Here,we used long-term collections of 33 flowering plant species from the Gongga Mountains(Mt.Gongga hereafter),a biodiversity hotspot,to investigate how plant flowering phenology changed over the past 70 years in response to climate change.We found that mean flowering times in Mt.Gongga were delayed in all vegetation types and elevations over the last 70 years.Furthermore,flowering time was delayed more in lowlands than at high elevations.Interestingly,we observed that spring-flowering plants show earlier flowering times whereas summer/autumn plants show delayed flowering times.Non-synchronous flowering phenology across species was mainly driven by changes in temperature and precipitation.We also found that the flowering phenology of 78.8%plant species was delayed in response to warming temperatures.Our findings also indicate that the magnitude and direction of variation in plant flowering times vary significantly among species along elevation gradients.Shifts in flowering time might cause trophic mismatches with co-occurring and related species,affecting both forest ecosystem structure and function.
基金the National Natural Science Foundation of China(32260379&32371852)the Jiangxi Provincial Natural Science Foundation(20224ACB215005)
文摘Global climate change can affect tree growth and carbon sink function by influencing plant carbohydrate synthesis and utilization,while elevation can be used as an ideal setting under natural conditions to simulate climate change effects.The effect of elevation on tree growth may depend on organ type.However,the allocation patterns of nonstructural and structural carbohydrates(NSCs and SCs,respectively)in different tree organs and their response to elevation remain unclear.We selected four dominant tree species,Schima superba,Castanopsis eyrei,Castanopsis fargesii and Michelia maudiae,along an elevation gradient from 609 to 1,207 m in subtropical evergreen broad-leaved forests and analyzed leaf,trunk,and fine root NSCs,carbon(C),nitrogen(N)and phosphorus(P)concentrations and the relative abundance of SCs.Leaf NSCs increased initially and then decreased,and trunk NSCs increased with increasing elevation.However,root NSCs decreased with increasing elevation.The relative abundance of SCs in leaves and trunks decreased,while the relative abundance of root SCs increased with increasing elevation.No significant correlations between SCs and NSCs in leaves were detected,while there were negative correlations between SCs and NSCs in trunks,roots,and all organs.Hierarchical partitioning analysis indicated that plant C/N and C/P were the main predictors of changes in SCs and NSCs.Our results suggest that tree organs have divergent responses to elevation and that increasing elevation will inhibit the aboveground part growth and enhance the root growth of trees.A tradeoff between the C distribution used for growth and storage was confirmed along the elevation gradient,which is mainly manifested in the"sink"organs of NSCs.Our results provide insight into tree growth in the context of global climate change scenarios in subtropical forest ecosystems.
基金This study was supported by the National Natural Science Foundation of China(No.U21A20270 and 32202079)Postdoctoral Science and Technology Project of Henan,Grant No.HN2022046+2 种基金Science and Technology Project of Henan Province(232103810064)the Innovative Funds Plan of Henan University of Technology(2021ZKCJ03)the Key Scientific Research Projects of Colleges and Universities of Henan(23A550012).
文摘The liquid-liquid extraction method using reverse micelles can simultaneously extract lipid and protein of oilseeds,which have become increasingly popular in recent years.However,there are few studies on mass transfer processes and models,which are helpful to better control the extraction process of oils and proteins.In this paper,mass transfer process of peanut protein extracted by bis(2-ethylhexyl)sodium sulfosuccinate(AOT)/isooctane reverse micelles was investigated.The effects of stirring speed(0,70,140,and 210 r/min),temperature of extraction(30,35,40,45,and 50℃),peanut flour particle size(0.355,0.450,0.600,and 0.900 mm)and solidliquid ratio(0.010,0.0125,0.015,0.0175,and 0.020 g/mL)on extraction rate were examined.The results showed that extraction rate increased with temperature rising,particle size reduction as well as solid-liquid ratio increase respectively,while little effect of stirring speed(P>0.05)was observed.The apparent activation energy of extraction process was calculated as 10.02 kJ/mol and Arrhenius constant(A)was 1.91 by Arrhenius equation.There was a linear relationship between reaction rate constant and the square of the inverse of initial particle radius(1/r_(0)^(2))(P<0.05).This phenomenon and this shrinking core model were anastomosed.In brief,the extraction process was controlled by the diffusion of protein from the virgin zone interface of particle through the reacted zone and it was in line with the first order reaction.Mass transfer kinetics of peanut protein extracted by reverse micelles was established and it was verified by experimental results.The results provide an important theoretical guidance for industrial production of peanut protein separation and purification.
基金National Natural Science Foundation of China(grant numbers 31870427&31730014)by Jiangxi Province 2019 Graduate Innovation Fund Project(grant number YC2019-b061)。
文摘Tree competitiveness generally depends on trait plasticity in response to environmental change.The effects of nitrogen(N)and phosphorus(P)on leaf trait variability by species is poorly understood,especially in China’s subtropical forests.This study examined the seedling leaf traits and net primary productivity of all trees>5 cm DBH of two dominant species,Schima superba and Castanopsis carlesii,in an evergreen broadleaved forest fertilized with nitrogen(+N),phosphorus(+P),and nitrogen plus phosphorus(N+P).The effect of N on seedling leaf traits was stronger than P,while fertilization in general was species dependent.Leaf mass per unit area decreased with N for S.superba seedlings but not for C.carlesii.Leaf N,P,and N/P ratios changed with N addition for both species.All four N fractions of carboxylation,bioenergetics,cell wall,and other N metabolites in C.carlesii leaves responded significantly to fertilization,while only the cell wall in S.superb a leaves responded.Other leaf functional traits,including light-saturated photosynthetic rates,water,N,and P use efficiencies,chlorophyll and non structural carbohydrate contents increased with N addition in S.superb a and by P addition in C.carlesii.Canopy closure at the stand-level increased due to N.Litter biomass and relative growth rate of S.superb a was not affected by any treatments,while both for C.carlesii significantly decreased with N+P addition.Collectively,nutrient limitation may vary at a small scale among species in a subtropical forest based on their responses of seedling traits and net primary productivity to fertilization.Seedling traits are not correlated with the net primary productivity of larger trees except for N fractions,because low light conditions induced by fertilization reduces the proportion of N allocated to photosynthesis in seedlings.In addition,acclimation differences of tree species may increase the uncertainty of community succession.
基金supported by the National Natural Science Foundation of China(Grant Nos.32171759,31730014).
文摘Background:The replanting of broadleaved trees in pure coniferous plantations is widely implemented,as mixed plantations are generally more stable and functional.However,the effect of interspecific interactions between broadleaved and coniferous trees on internal nutrient cycles of conifers remains unclear.Methods:We selected pure coniferous plantations of a native(Pinus massoniana)and an exotic(P.elliottii)pine species and their corresponding mixed plantations with broadleaved trees(Schima superba)in subtropical China,and measured the nitrogen(N)and phosphorus(P)contents in the rhizosphere soils,fine roots,twigs,needles and needle litter of pines.We calculated the root capture,needle resorption and translocation of N and P by pines to determine the mobility of nutrients in trees.Results:Although the N and P in the rhizosphere soils increased due to the replanting of broadleaved trees,the N and P contents in the aboveground tissues of the two pine species did not increase in mixed plantations.Mixed planting had a negative effect on the N and P capture of native pine and a positive effect on that of exotic pine.The N and P resorption efficiencies increased in native pine but were unchanged in exotic pine after the replanting of S.superba.Native pine preferentially employed an aboveground nutrient resorption strategy,whereas exotic pine tended to adopt a belowground nutrient capture strategy after replanting.Translocation of N and P in trees was detected,which reflected the trade-offs between root nutrient capture and needle nutrient resorption.Conclusions:The effect of mixed planting varied between the species of native and exotic pines,and the internal nutrient cycles of both pine species might be dominated by interspecific interaction effects on nutrients rather than soil nutrients.Our study highlights the importance of selecting suitable broadleaved species for replanting in coniferous plantations.
基金jointly financed by the Programs of the National Natural Science Foundation of China(Nos.41571251,41571130043)the Major State Basic Research Development Program of China(No.2012CB416903)
文摘Nitrogen (N) and phosphorus (P) additions can affect soil microbial carbon (C) accumulation. However, the mechanisms that drive the changes in residual microbial C that occur after N and P additions have not been well-defined for Chinese fir plantations in subtropical China. We set up six different treatments, viz. a control (CK), two N treatments (NI: 50kgha-1 a-1; N2: 100 kg ha-1 a-1), one P treatment (P: 50 kg ha-1 a-1), and two combined N and P treatments (NIP: 50kgha-1a-1 of N +50kgha-1a-1 of P; N2P:100 kg ha-1 a-1 of N + 50 kg ha-1 a-1 of P). We then investigated the influences of N and P additions on residual microbial C. The results showed that soil pH and microbial biomass decreased after N additions, while microbial biomass increased after P additions. Soil organic carbon (SOC) and residual microbial C contents increased in the N and P treatments but not in the control. Residual microbial C accumulation varied according to treatment and declined in the order: N2P 〉 N1P 〉 N2 〉 N1 〉 P 〉 CK. Residual microbial C contents were positively correlated with available N, P, and SOC contents, but were negatively correlated with soil pH. The ratio of residual fungal C to residual bacterial C increased under P additions, but declined under combined N1P additions. The ratio of residual microbial C to SOC increased from 11 to 14% under the N1P and N2P treatments, respectively. Our results suggest that the concentrations of residual microbial C and the stability of SOC would increase under combined applications of N and P fertilizers in subtropical Chinese fir plantation soils.
基金the National Key R&D Program of China(Grant No.2017YFA0304300)the Chinese Academy of Sciences+6 种基金Anhui Initiative in Quantum Information TechnologiesTechnology Committee of Shanghai Municipalitythe National Natural Science Foundation of China(Grants Nos.11905217,11774326,and 11905294)the Shanghai Municipal Science and Technology Major Project(Grant No.2019SHZDZX01)the Natural Science Foundation of Shanghai(Grant No.19ZR1462700)the Key-Area Research and Development Program of Guangdong Provice(Grant No.2020B0303030001)the Youth Talent Lifting Project(Grant No.2020-JCJQ-QT-030)。
文摘High-fidelity two-qubit gates are essential for the realization of large-scale quantum computation and simulation.Tunable coupler design is used to reduce the problem of parasitic coupling and frequency crowding in manyqubit systems and thus thought to be advantageous. Here we design an extensible 5-qubit system in which center transmon qubit can couple to every four near-neighboring qubits via a capacitive tunable coupler and experimentally demonstrate high-fidelity controlled-phase(CZ) gate by manipulating central qubit and one nearneighboring qubit. Speckle purity benchmarking and cross entropy benchmarking are used to assess the purity fidelity and the fidelity of the CZ gate. The average purity fidelity of the CZ gate is 99.69±0.04% and the average fidelity of the CZ gate is 99.65±0.04%, which means that the control error is about 0.04%. Our work is helpful for resolving many challenges in implementation of large-scale quantum systems.
基金the Science and Technology Project of Henan Province(212102110320)the Science Foundation of Henan University of Technology(Grant Nos.2020BS013,2019BS029)
文摘Foodborne pathogen poses a threat to the food industries as many outbreaks have been found to be associated with biofilm formation.The formation of biofilm is a self-protection growth pattern of bacteria,which increases post-processing contamination and risk to public health.It is difficult to eliminate the biofilm in the food industries,since the biofilm cells have a barrier preventing or lessening the contact with environmental stresses,antimicrobial agents and the host immune system.Bacterial biofilm formation is a complex process,including initial attachment stage,irreversible attachment stage,biofilm development stage,biofilm maturation stage,and biofilm dispersion stage.The genetic mechanism,substratum and bacterial cell surface properties involve in the biofilm formation.The biofilm inhibition methods studied are physical treatment,chemical and biochemical treatment.The potential green and safe biochemical method attracts more attention,especially,the novel strategies using the safe biochemical agents(essential oils,enzymes,biosurfactants,others)constantly emerged.The review emphasized on effective strategies for inhibiting biofilm formation in different stages(initial irreversible attachment,formation,and maturation)by use of biochemical agents,aiming to provide new insight into biofilm control in food industry thus improving food quality and safety.
基金the USTC Center for Micro-and Nanoscale Research and Fabrication for supporting the sample fabricationQuantum CTek Co.,Ltd.for supporting the fabrication and the maintenance of room-temperature electronics+7 种基金supported by the National Key R&D Program of China(Grant No.2017YFA0304300)the Chinese Academy of Sciencesthe Anhui Initiative in Quantum Information Technologiesthe Technology Committee of Shanghai Municipalitythe National Natural Science Foundation of China(Grants No.11905217 and 11905294)the Natural Science Foundation of Shanghai(Grant No.19ZR1462700)he Key-Area Research and Development Program of Guangdong Province(Grant No.2020B0303030001)the China Postdoctoral Science Foundation。
文摘The development of high-fidelity two-qubit quantum gates is essential for digital quantum computing.Here,we propose and realize an all-microwave parametric controlled-Z(CZ)gates by coupling strength modulation in a superconducting Transmon qubit system with tunable couplers.After optimizing the design of the tunable coupler together with the control pulse numerically,we experimentally realized a 100 ns CZ gate with high fidelity of 99.38%±0.34%and the control error being 0.1%.We note that our CZ gates are not affected by pulse distortion and do not need pulse correction,providing a solution for the real-time pulse generation in a dynamic quantum feedback circuit.With the expectation of utilizing our all-microwave control scheme to reduce the number of control lines through frequency multiplexing in the future,our scheme draws a blueprint for the high-integrable quantum hardware design.
文摘Sericin silkworm is a kind of gene mutant silkworm that can only synthesis and secrete sericin protein instead of fibroin protein. In this study, according to the knowledge of physiology and heredity, the reasons of this special phenomenon for the sericin silkworm were analyzed in order to explore the utilization clues in the production of natural sericin and product development.
基金supported by the Chinese National Science Foundational Project(32160292,32171759,and 31930070)the National Key Research and Development Program of China(2017YFA0604403 and 2016YFA0600804)+2 种基金JIANGXI DOUBLE THOUSAND PLANS(jxsq2020101080)the Natural Science Foundation of Jiangxi province(20224BAB205008)supported by University of New Hampshire。
文摘Atmospheric vapor pressure deficit(VPD)increases with climate warming and may limit plant growth.However,gross primary production(GPP)responses to VPD remain a mystery,offering a significant source of uncertainty in the estimation of global terrestrial ecosystems carbon dynamics.In this study,in-situ measurements,satellite-derived data,and Earth System Models(ESMs)simulations were analysed to show that the GPP of most ecosystems has a similar threshold in response to VPD:first increasing and then declining.When VPD exceeds these thresholds,atmospheric drought stress reduces soil moisture and stomatal conductance,thereby decreasing the productivity of terrestrial ecosystems.Current ESMs underscore CO_(2) fertilization effects but predict significant GPP decline in low-latitude ecosystems when VPD exceeds the thresholds.These results emphasize the impacts of climate warming on VPD and propose limitations to future ecosystems productivity caused by increased atmospheric water demand.Incorporating VPD,soil moisture,and canopy conductance interactions into ESMs enhances the prediction of terrestrial ecosystem responses to climate change.
基金the National Key R&D Program of China(2017YFA0304300),the Chinese Academy of Sciences,Anhui Initiative in Quantum Information Technologies,Technology Committee of Shanghai Municipality,National Natural Science Foundation of China(11905217,11774326,and 11905294)‘Shang-hai Municipal Science and Technology Major Project(2019SHZDZX01)’Natural Science Foundation of Shanghai(19ZR1462700)‘Key-Area Research and Development Program of Guangdong Province(2020B0303030001)’the Youth Talent Lifting Project(2020-JCJQ-QT-030)。
文摘To ensure a long-term quantum computational advantage,the quantum hardware should be upgraded to withstand the competition of continuously improved classical algorithms and hardwares.Here,we demonstrate a superconducting quantum computing systems Zuchongzhi 2.1,which has 66 qubits in a two-dimensional array in a tunable coupler architecture.The readout fidelity of Zuchongzhi 2.1 is considerably improved to an average of 97.74%.The more powerful quantum processor enables us to achieve larger-scale random quantum circuit sampling,with a system scale of up to 60 qubits and 24 cycles,and fidelity of FXEB=(3·66±0·345)×10^(-4).The achieved sampling task is about 6 orders of magnitude more difficult than that of Sycamore[Nature 574,505(2019)]in the classic simulation,and 3 orders of magnitude more difficult than the sampling task on Zuchongzhi 2.0[arXiv:2106.14734(2021)].The time consumption of classically simulating random circuit sampling experiment using state-of-the-art classical algorithm and supercomputer is extended to tens of thousands of years(about 4·8×104years),while Zuchongzhi 2.1 only takes about 4.2 h,thereby significantly enhancing the quantum computational advantage.
基金The authors acknowledged that this work was financially supported by Major Scientific and Technological Project of Henan Province(Grant No.161100110600)Key Scientific and Technological Project of Henan Province(No.212102310491,No.182102310060)+3 种基金China Postdoctoral Science Foundation(No.2018M632767)Henan Postdoctoral Science Foundation(No.001801021)Youth Talents Support Project of Henan Province(No.2018HYTP008)and Bainong Outstanding Talents Project of Henan Institute of Science and Technology(No.BNYC2018-2-27).
文摘Pseudomonas spp.and Enterobacteriaceae are dominant spoilage bacteria in chicken during cold storage(0°C-4°C).In this study,high resolution spectra in the range of 900-1700 nm were acquired and preprocessed using Savitzky-Golay convolution smoothing(SGCS),standard normal variate(SNV)and multiplicative scatter correction(MSC),respectively,and then mined using partial least squares(PLS)algorithm to relate to the total counts of Pseudomonas spp.and Enterobacteriaceae(PEC)of fresh chicken breasts to predict PEC rapidly.The results showed that with full 900-1700 nm range wavelength,MSC-PLS model built with MSC spectra performed better than PLS models with other spectra(RAW-PLS,SGCS-PLS,SNV-PLS),with correlation coefficient(RP)of 0.954,root mean square error of prediction(RMSEP)of 0.396 log10 CFU/g and residual predictive deviation(RPD)of 3.33 in prediction set.Based on the 12 optimal wavelengths(902.2 nm,905.5 nm,923.6 nm,938.4 nm,946.7 nm,1025.7 nm,1124.4 nm,1211.6 nm,1269.2 nm,1653.7 nm,1691.8 nm and 1693.4 nm)selected from MSC spectra by successive projections algorithm(SPA),SPA-MSC-PLS model had RP of 0.954,RMSEP of 0.397 log10 CFU/g and RPD of 3.32,similar to MSC-PLS model.The overall study indicated that NIR spectra combined with PLS algorithm could be used to detect the PEC of chicken flesh in a rapid and non-destructive way.
基金The authors would like to acknowledge the financial support provided by Major Scientific and Technological Project of Henan Province(No.161100110600)China Postdoctoral Science Foundation(No.2018M632767)+3 种基金Key Scientific and Technological Project of Henan Province(No.182102310060,No.182102110091)Youth Talents Lifting Project of Henan Province(No.[2017]132-08)Key Scientific Research Project of Henan Province(No.18A550007)National Natural Science Foundation of China(No.31860465).
文摘Total viable count(TVC)is often used as an important indicator for chicken freshness evaluation.In this study,112 fresh chicken flesh samples were acquired after slaughtered and their hyperspectral images were collected in the LW-NIR(900-1700 nm)range.The full LW-NIR spectra(486 wavebands)within the images were extracted and applied to related to reference TVC values measured in different storage periods,using partial least squares regression(PLSR)algorithm,resulting in high correlation coefficients(R)and low root mean square errors(RMSE),for either raw spectra or pretreatment spectra.By using regression coefficients(RC)method,20,18,17 and 20 optimal wavebands were respectively selected from raw spectra,baseline correction(BC)spectra,Savitzky-Golay convolution smoothing(SGCS)spectra and standard normal variate(SNV)spectra and applied for the optimization of original full waveband PLSR model.By comparison,RC-PLSR model based on the SGCS spectra showed a better performance in TVC prediction with RC of 0.98 and RMSEC of 0.35 log10 CFU/g in calibration set,and RP of 0.98 and RMSEP of 0.44 log10 CFU/g in prediction set.At last,by transferring the best RC-PLSR model,the dynamic TVC change during the storage was visualized by color maps to indicate the TVC spoilage degree.The overall study revealed that LW-NIR hyperspectral imaging combined with PLSR could be used to predict the freshness of chicken flesh.
基金The authors acknowledge that this work was financially supported by the Key Scientific and Technological Project of Henan Province(Grant No.212102310491,No.182102310060)Major Scientific and Technological Project of Henan Province(No.161100110600)+2 种基金China Postdoctoral Science Foundation(No.2018M632767)Henan Postdoctoral Science Foundation(No.001801021)Youth Talents Lifting Project of Henan Province(No.2018HYTP008).
文摘Accurate and rapid determination of nitrite contents is an important step for guaranteeing sausage quality.This study attempted to mine hyperspectral data in the range of 900-1700 nm for non-destructive and rapid prediction of nitrite contents in sausages.The average spectra of 156 samples were collected to relate to the measured nitrite values by partial least squares(PLS)regression.Optimal wavelengths were respectively selected by successive projections algorithm(SPA)and regression coefficients(RC)to simplify the PLS model.The results indicated that PLS model established with 15 optimal wavelengths(900.5 nm,907.1 nm,908.8 nm,912.1 nm,915.4 nm,920.3 nm,922.0 nm,941.7 nm,979.6 nm,1083.2 nm,1213.2 nm,1353.0 nm,1460.2 nm,1595.6 nm and 1699.9 nm)selected by SPA had better performance with r C,r CV,r P of 0.92,0.89,0.89 and RMSEC,RMSECV,RMSEP of 0.41 mg/kg,0.89 mg/kg,0.49 mg/kg,respectively,for calibration set,cross-validation and prediction set.It was concluded that hyperspectral data could be mined by PLS&SPA for realizing the rapid evaluation of nitrite content in ham sausages.
基金supported by Innovation Program for Quantum Science and Technology (2021ZD0300200)Shanghai Municipal Science and Technology Major Project (2019SHZDZX01)+13 种基金Special funds from Jinan Science and Technology Bureau and Jinan High Tech Zone Management Committeethe Chinese Academy of Sciences (CAS)Anhui Initiative in Quantum Information TechnologiesTechnology Committee of Shanghai MunicipalityNatural Science Foundation of Shandong Province (ZR202209080019)Key-Area Research and Development Program of Guangdong Provice (2020B0303030001)supported in part by the Japanese MEXT Quantum Leap Flagship Program (MEXT Q-LEAP,JPMXS0118069605)the support from the Youth Talent Lifting Project (2020-JCJQ-QT-030)the National Natural Science Foundation of China (12274464,and 11905294)China Postdoctoral Science Foundationthe Open Research Fund from State Key Laboratory of High Performance Computing of China (201901-01)supported by Shanghai Rising-Star Program (23QA1410000)the Youth Innovation Promotion Association of CAS (2022460)the support from THE XPLORER PRIZE。
基金supported by the National Natural Science Foundation of China (No. 41630862)the National Key Research and Development Program (No. 2017YFD0200100)the “China Soil Microbiome Initiative: Function and Regulation of Soil—Microbial Systems” of the Chinese Academy of Sciences (No. XDB15040200)。
文摘Increasing atmospheric CO2 concentration impacts the terrestrial carbon(C) cycle by affecting plant photosynthesis, the flow of photosynthetically fixed C belowground, and soil C pool turnover. For managed agroecosystems, how and to what extent the interactions between elevated CO2 and N fertilization levels influence the accumulation of photosynthesized C in crops and the incorporation of photosynthesized C into arable soil are in urgent need of exploration.We conducted an experiment simulating elevated CO2 with spring wheat(Triticum aestivum L.) planted in growth chambers.13C-enriched CO2 with an identical 13C abundance was continuously supplied at ambient and elevated CO2 concentrations(350 and 600 μmol mol-1, respectively) until wheat harvest.Three levels of N fertilizer application(equivalent to 80, 120, and 180 kg N ha-1 soil) were supplied for wheat growth at both CO2 concentrations. During the continuous 62-d 13CO2 labeling period, elevated CO2 and increased N fertilizer application increased photosynthesized C accumulation in wheat by 14%–24% and 11%–20%, respectively, as indicated by increased biomass production, whereas the C/N ratio in the roots increased under elevated CO2 but declined with increasing N fertilizer application levels. Wheat root deposition induced 1%–2.5% renewal of soil C after 62 d of 13CO2 labeling. Compared to ambient CO2, elevated CO2 increased the amount of photosynthesized C incorporated into soil by 20%–44%. However, higher application rates of N fertilizer reduced the net input of root-derived C in soil by approximately 8% under elevated CO2. For the wheat-soil system, elevated CO2 and increased N fertilizer application levels synergistically increased the amount of photosynthesized C. The pivotal role of plants in photosynthesized C accumulation under elevated CO2 was thereby enhanced in the short term by the increased N application. Therefore, robust N management could mediate C cycling and sequestration by influencing the interactions between plants and soil in agroecosystems under elevated CO2.
基金funded by the National Natural ScienceFoundationof China(31770749,31760200)Jiangxi Province Science Foundation for Youths(20181BAB214014)Project of Jiangxi Education Department(GJJ190173).
文摘Litter decomposition impacts carbon(C)and nutrient cycling.Nitrogen(N)and phosphorus(P)addition as well as litter age impact litter decomposition.Effects of nutrient addition and litter age on litter decomposition may impact emissions of soil nitrous oxide(N_(2)O),which is an important greenhouse gas.However,no study has examined the effects of interactions between litter age and nutrient addition on soil N_(2)O emissions,and explored the underlining mechanisms simultaneously,thus limiting our evaluation of litter decomposition effects on N_(2)O emissions.Litter with different age was collected from Cunninghamia lanceolata plantations experienced N and P addition treatments to examine the effects of nutrient addition and litter age on N_(2)O emissions by incubation study.Litter age generally increased N_(2)O emissions via a decrease in litter C:N ratio.While P addition decreased N_(2)O emissions,N addition increased them mainly by positive effects on soil enzymes as indicated by microbial functional genes associated with N_(2)O production and negative effects on litter C:N ratio.Litter age and nutrient addition interacted to impact soil N_(2)O emissions.In future forest management,both nutrient addition and litter age should be considered in evaluation of management effects on N_(2)O emissions,especially thinning or selectively cutting involving litter input with different age.
文摘This study insightfully analyzed the relationship between chemical composition change,volatile flavor compounds(VFCs)and microbial structural distribution of Chinese-style(CFS)and Salami fermented sausages(WFS)during their fermentation processes(FP).The results showed pH,acid and peroxide values of CFS were higher than WFS during whole FP(38 d).At the end of fermentation(38 days),the pH value of CFS and WFS were 5.70±0.01 and 5.43±0.01,respectively.The acid value was 9.89±0.38 and 6.29±0.28 mg/g,respectively.The peroxide value was 0.21±0.01 and 0.14±0.01 g/100g,respectively.During 0-13 d,moisture contents of CFS were higher than WFS,while opposite in 14-38 d.Nitrite contents of WFS were higher than CFS.The total free-amino acid contents of CFS were significantly lower than WFS(p<0.05).The six groups of main VFCs in the two sausages were aldehydes,acids,alcohols,esters,ketones and hydrocarbons,respectively,and the contents of ketone in CFS were higher than WFS.However,the bacterial abundance in CFS increased gradually,while that in WFS’s increased firstly and then decreased.The abundances and diversities of fungi in WFS were higher than CFS.In brief,microbial structural distribution formed an obvious impact on the chemical composition quality of fermented sausages,the above results provided data support for the production improvement of Chinese-style and Salami fermented sausages.