【目的】研究绿肥、稻草不同还田模式对湘南地区水稻产量和土壤肥力的影响,为绿肥、稻草还田技术的推广和土壤培肥提供理论依据。【方法】2013—2018年在湘南红壤地区开展了6年田间小区定位试验,共设置6个处理,分别为冬闲+稻草不还田(CK...【目的】研究绿肥、稻草不同还田模式对湘南地区水稻产量和土壤肥力的影响,为绿肥、稻草还田技术的推广和土壤培肥提供理论依据。【方法】2013—2018年在湘南红壤地区开展了6年田间小区定位试验,共设置6个处理,分别为冬闲+稻草不还田(CK)、冬种紫云英+稻草不还田(MV)、冬闲+早稻草全部还田(RS1)、冬闲+早、晚稻草全部还田(RS2)、冬种紫云英+早稻草全部还田(RS3)、冬种紫云英+早稻草全部还田+晚稻留高茬还田(RS4),各处理均施等量化肥。每季收获后测产,计算平均产量、产量变异系数和可持续指数。2017年晚稻收获后,取0—20 cm土层土壤样品,测定其养分含量和水稳性团聚体含量。【结果】与CK相比,除R S 1处理外,其余处理早稻、晚稻和周年平均产量均显著提高,增幅分别为6.6%~24.6%、11.9%~28.8%和9.0%~26.5%,以RS4处理增产效果最显著,其晚稻和周年平均产量均显著高于其他还田处理。与CK相比,除RS1处理早稻和MV处理晚稻外,其余处理早、晚稻产量变异系数均下降,且均以RS3处理最低;除晚稻MV处理外,其余处理早、晚稻产量可持续系数保持不变或提高,且早、晚稻均以RS3处理最高。试验开展5年后,各处理土壤有机质含量与初始土壤相比均明显提高,绿肥、稻草还田处理除RS1外均显著高于CK,RS4处理还显著高于RS1处理;各处理土壤全氮含量较2013年均有所降低,但绿肥、稻草还田处理较CK下降幅度小,MV和RS3处理全氮含量显著高于CK。土壤速效钾含量除MV和RS3处理低于CK外,其他处理均有不同程度的增加。粒径>0.25 mm水稳性团聚体以RS2处理最高,达68.9%。【结论】绿肥联合早稻草全部还田和晚稻留高茬还田措施可提高土壤有机质含量,稳定土壤氮素供应的长期效果显著,是湘南红壤地区水稻高产稳产和可持续发展相对较好的耕作制度,能够有效促进水稻增产,改善土壤理化性质,稳定氮素供应。展开更多
种子被视为有益微生物的储存库和载体。与根际和叶际相比,种子相关细菌的研究还十分落后。本研究以长期绿肥植物与水稻轮作(稻-稻-紫云英,R-R-MV)和冬闲(稻-稻-冬闲,R-R-WF)两种种植模式的水稻种子为材料,分离种子内生细菌;在对菌株进行...种子被视为有益微生物的储存库和载体。与根际和叶际相比,种子相关细菌的研究还十分落后。本研究以长期绿肥植物与水稻轮作(稻-稻-紫云英,R-R-MV)和冬闲(稻-稻-冬闲,R-R-WF)两种种植模式的水稻种子为材料,分离种子内生细菌;在对菌株进行16S r RNA基因系统发育分析基础上,依据分离来源和分类地位选择代表菌株,进行溶磷、产IAA和铁载体等植物促生功能的检测。结果显示,绿肥轮作后水稻种子内生细菌的数量显著高于冬闲,群落组成也发生了明显变化。在属水平上,Pseudomonas和Xanthomonas是R-R-WF中的优势属,分别占28.57%和26.19%,而R-R-MV中Pantoea是优势属,占27.94%,其次是Pseudomonas(14.71%)和Paenibacillus(13.24%)。进行功能检测的31个菌株中,大多数分离株具有有益于植物的特性,表明种子内生细菌与植物的生长及健康密切相关。本研究不仅揭示了长期轮作条件可以改变种子的微生物组成,还获得了有益的微生物资源,将有助于种子内生菌作为生物接种剂在可持续农业中的研究和应用。展开更多
Soil physical properties are important indicators of the potential for agricultural production.Our objective was to evaluate the effects of long-term inputs of green manures on physical properties of a reddish paddy s...Soil physical properties are important indicators of the potential for agricultural production.Our objective was to evaluate the effects of long-term inputs of green manures on physical properties of a reddish paddy soil(Fe-Typic Hapli-Stagnic Anthrosols) under a double cropping system.The common cropping pattern before the study was early-late rice-fallow(winter).The field treatments included rice-rice-fallow(R-R-WF),rice-rice-rape(R-R-RP),rice-rice-Chinese milk vetch(RR-MV),and rice-rice-ryegrass(R-R-RG).The rape,Chinese milk vetch and ryegrass were all incorporated as green manures 15 d before early rice transplanting during the following year.The soil bulk density in all green manure treatments was significantly reduced compared with the winter fallow treatment.Soil porosity with green manure applications was significantly higher than that under the winter fallow.The green manure treatments had higher 0.25-5 mm water stable aggregates and aggregates stabilities in the plow layer(0-15 cm depth) compared with the fallow treatment.The mean weight diameter(MWD) and normalized mean weight diameter(NMWD) of aggregates in the green manure treatment were larger than that with the winter fallow.Soil given green manure retained both a higher water holding capacity in the plow layer soil,and a larger volume of moisture at all matric potentials(-10,-33 and-100 kPa).We conclude that the management of double-rice fields in southern central China should be encouraged to use green manures along with chemical fertilizers to increase SOC content,improve soil physical properties and soil fertility.展开更多
Dissolved organic matter(DOM) plays important roles in soil biogeochemistry activity and nutrients transportation in soils, but studies regarding the long-term effects of green manures on the content and structure of ...Dissolved organic matter(DOM) plays important roles in soil biogeochemistry activity and nutrients transportation in soils, but studies regarding the long-term effects of green manures on the content and structure of DOM in red paddy soil have not been reported yet. A long-term green manure experiment established in 1982 was utilized to test the DOM contents in different treatments, and the spectral characteristics of DOM were investigated by using ultraviolet-visible(UV-Vis) spectrometry and Fourier transform infrared(FTIR) spectrometry. The experiment included four cropping systems: ricerice-milk vetch(RRV), rice-rice-rape(RRP), rice-rice-ryegrass(RRG) and rice-rice-winter fallow(RRF), among them, milk vetch, rape, and ryegrass are popular winter green manure species in southern China. The results showed that the content of dissolved organic carbon(DOC), which is widely used to estimate the concentration of DOM, was significantly promoted after the incorporation of green manures compared with the other sampling stages. The contents of aromatic groups and the degree of humification of DOM increased in RRV and RRP, suggesting more complex compositions of the soil DOM after long-term application of milk vetch and rape. The contents of phenol, alcohol and carboxylic acid group at the mature stage of early rice were significantly higher than those at the stage of after green manures turned over, especially for the RRV treatment. The absorption ratio of FTIR indicated that winter plantation of rape increased the aromatic-C/aliphatic-C ratio, and ryegrass increased the aromatic-C/carboxyl-C ratio. In conclusion, long-term planting of milk vetch and rape as green manures increased the degree of aromaticity, humification and average molecular weight of DOM, and made the DOM more stable in red paddy soil.展开更多
The content of organic matter (OM), nitrogen (N), phosphorus (P), and potassium (K) in the three selected soils, including Calcaric Purpli-Orthic Primosols (purple sand-shale parent material), Dystric Turbi-...The content of organic matter (OM), nitrogen (N), phosphorus (P), and potassium (K) in the three selected soils, including Calcaric Purpli-Orthic Primosols (purple sand-shale parent material), Dystric Turbi-Anthric Primosols (quaternary red clay parent material) and Typic Udi-Sandic Primosols (granite parent material) were studied under a long-term experiment by using crop straw and inorganic fertilizers at the Hunan Red Soil Experiment Station of Chinese Academy of Agricultural Sciences. The results showed that the contents of OM, N, P and K in the three selected soils increased after 23 years application of crop straw and inorganic fertilizers, but the contents increased much less when crop straw or inorganic fertilizers was applied alone. The nutrient contents in the three soils developed from granite changed more remarkably than those in the soil derived from quaternary red clay and purple sand-shale. It was also found that the contents of OM, N, and P increased slightly in the treatments without applying fertilizers or returning the crop straw to the root bed. Combined application of inorganic fertilizers and crop straw could remarkably increase the contents of OM, alkalihydrolyzable N and available K, the positive correlation between application of organic fertilizers and increase of OM in soil did not always happened, it provided evidence for the relation between appropriate C/N ratio and accumulation of OM in soil. The increase of nutrient content was influenced by the soil properties. By comparing the contents of nutrient in 0-20 cm depth in the three different soils, it was concluded that the most increases of OM, alkali-hydrolyzable N, and available P were observed in Typic Udi-Sandic Primosols with the average increase by 3.03, 27.38, and 21.73 mg kg^-1, respectively. The available K increased in Dystric Turbi-Anthric Primosols with the average increase by 25.82 mg kg^-1, while it decreased in Calcaric Purpli-Orthic Primosols and Typic Udi-Sandic Primosols. It was concluded that the application of inorganic fertilizer and crop straw was important to improve the soil fertility for all of three selected soils. The straw return to the field had played a significant role for enhancement of the soil quality in the study areas. The application of inorganic fertilizer combined with the straw return to the fields could remarkably improve the soil fertility.展开更多
Dissimilatory Fe(Ⅲ) reduction is an important process in the geochemical cycle of iron in anoxic environment. As the main products of dissimilatory iron reduction, the Fe(Ⅱ) species accumulation could indicate t...Dissimilatory Fe(Ⅲ) reduction is an important process in the geochemical cycle of iron in anoxic environment. As the main products of dissimilatory iron reduction, the Fe(Ⅱ) species accumulation could indicate the reduction ability. The effects of different green manures on Fe(Ⅲ) reduction in paddy soil were explored based on a 31-year rice-rice-winter green manure cropping experiment. Four treatments were involved, i.e., rice-rice-milk vetch (RRV), rice-rice-rape (RRP), rice-rice-ryegrass (RRG) and rice-rice-winter fallow (RRF). Soils were sampled at flowering stage of milk vetch and rape (S1), before transplantation (S2), at tillering (S3), jointing (S4), and mature (S5) stages of the early rice, and after the harvest of the late rice (S6). The contents of TFeHa (HCI-extractable total Fe), Fe(Ⅱ)HCI (HCI-extractable Fe(Ⅱ) species) and Fe(Ⅲ)HCI (HCI- extractable Fe(Ⅲ) species) were measured. The correlations among those Fe species with selected soil environmental factors and the dynamic characteristics of Fe(Ⅱ)HCI accumulation were investigated. The results showed that TFeHc~ in RRF was significantly higher than those in the green manure treatments at most of the sampling stages. Fe(II)Ha increased rapidly after the incorporation of green manures in all treatments and kept rising with the growth of early rice. Fe(Ⅱ)Ha in RRG was quite different from those in other treatments, i.e., it reached the highest at the S2 stage, then increased slowly and became the lowest one at the S4 and S5 stages. Fe(Ⅲ)Ha showed oppositely, and Fe(Ⅱ)HCI/Fe(Ⅲ)HCI performed similarly to Fe(Ⅱ)HCI The maximum accumulation potential of Fe(Ⅱ)HCI was significantly higher in RRF, while the highest maximum reaction rate of Fe(Ⅱ)Ha accumulation appeared in RRG. Significant correlations were found between the indexes of Fe(Ⅱ)HCI accumulation and soil pH, oxidation-reduction potential (Eh) and total organic acids, respectively. In together, we found that long-term application of green manures decreased the TFeHa in red paddy soils, but promoted the ability of Fe(lll) reduction, especially the ryegrass; Fe(Ⅱ)Ha increased along with the growth of rice and was affected by soil conditions and environmental factors, especially the water and redox ability.展开更多
On the basis of a long-term(30 years) field experiment that involved four rotation systems, rice-rice-winter fallow(RRF), rice-rice-ryegrass(RRG), rice-rice-rape(RRP), and rice-rice-milk vetch(RRV), this stu...On the basis of a long-term(30 years) field experiment that involved four rotation systems, rice-rice-winter fallow(RRF), rice-rice-ryegrass(RRG), rice-rice-rape(RRP), and rice-rice-milk vetch(RRV), this study described the effects of green manure on the microbial communities in the red paddy soils using 454 pyrosequencing for the 16 S r RNA gene. The Chao1 richness and non-parametric Shannon's index increased in all soil samples that received green manure treatments. The communities' structures with the green manure applications were significantly dissimilar from that under the winter fallow. Using Metastats tests, many genera in the RRG, RRP and RRV soils were significantly different from those in the RRF soil, including a number of genera that functioned in the nitrogen and sulfur cycles. Analyses of the genera with these functions revealed the shifts in microbial ecosystem functions after long-term green manuring. Changes in the microbial communities increased the ammonium supply and decreased the soil acidification in green-manure-amended soils. Together, these data suggested powerful effects of green manure on both the microbial communities and the biogeochemical cycle driven by the shifts in bacterial functional groups.展开更多
Effects of two kinds of magnesium compound with fertilizer on Daylily (Hemerocallis citrina Baroni) growth, yield, and soil nutrients in red soil were studied. The results indicated that significant effects of magne...Effects of two kinds of magnesium compound with fertilizer on Daylily (Hemerocallis citrina Baroni) growth, yield, and soil nutrients in red soil were studied. The results indicated that significant effects of magnesium applied to soils were observed in increasing Daylily (Hemerocallis citrina Baroni) yield, improving its growth, and strengthening its antivirus property as well as increasing the amount of exchangeable Mg, N, P, and K in red soil. In particular, the effects of magnesium compound fertilizer Ⅱ (MCF2) with higher Mg content were better than that of the others, which increased Daylily (Hemerocallis citrina Baroni) yield by 57.4, 32.8, and 14.5% compared to that of control treatment (CK), chemical fertilizer with nitrogen, phosphorus, potassium treatment (CF), and magnesium compound fertilizer Ⅰ treatment (MCF1) with lower Mg content. It increased soil Alkali N, available P, exchangeable K, and exchangeable Mg by 94.9, 46.5, 31.1, and 35.3%, respectively, compared with that of CK treatment. Therefore, the application of magnesium compound with fertilizer is an optimum method for improving red soil quality.展开更多
Fertilization is an effective technique to improve soil fertility and increase crop yield.The long-term effects of different fertilizers on soil considerably vary.Over 38 consecutive years of different fertilization p...Fertilization is an effective technique to improve soil fertility and increase crop yield.The long-term effects of different fertilizers on soil considerably vary.Over 38 consecutive years of different fertilization positioning experiments in a double cropping rice field of Qiyang Red Soil Experimental Station,seven different fertilization treatments including CK(no fertilization),NPK(nitrogen,phosphorus,and potassium fertilizer),M(cow manure),NPKM(nitrogen,phosphorus,and potassium with cow manure),NPM(nitrogen and phosphorus with cow manure),NKM(nitrogen and potassium with cow manure),and PKM(phosphorus and potassium with cow manure)were applied to study the effects on rice yield,soil fertility,and nutrient apparent balance in a paddy field.The results showed that the annual average yields of rice in NPKM,NPM,NKM,PKM,M,NPK and CK treatments ranged from 6214 to 11562 kg ha-1.Yields under long-term organic and inorganic treatments(NPKM,NPM,NKM and PKM)were 22.58,15.35,10.53 and 4.41%,respectively,greater than under the NPK treatment.Soil organic carbon(SOC),total nitrogen(TN),available nitrogen(AN)and available potassium(AK)concentration with long-term organic and inorganic treatment(NPKM,NPM,NKM and PKM)were significantly higher than in inorganic fertilizer(NPK)treatments.Soil total phosphorus(TP)and available phosphorus(AP)contentration with organic fertilizer combined with inorganic N and P fertilizer treatment(NPKM,NPM and PKM)were significantly higher than with inorganic fertilizer alone(NPK treatments).The average annual rice yield(11562 kg ha-1),SOC(20.88 g kg-1),TN(2.30 g kg-1),TP(0.95 g kg-1),TK(22.50 g kg-1)and AP(38.94 mg kg-1)concentrations were the highest in the NPKM treatment.The soil AN concentration(152.40 mg kg-1)and AK contentration(151.00 mg kg-1)were the highest in the NKM treatment.N and P application led to a surplus of nitrogen and phosphorus in the soil,but NPKM treatment effectively reduced the surplus compared with other treatments.Soils under all treatments were deficient in potassium.Correlation analysis showed that SOC,TN,AN,TP,and AP contentration was significantly correlated with rice yield;the correlation coefficients were 0.428,0.496,0.518,0.501,and 0.438,respectively.This study showed that the combined application of N,P,and K with cow manure had important effects on rice yield and soil fertility,but balanced application of N,P,and K with cow manure was required.展开更多
The effects of the cropping system change for paddy field with double harvest rice on crops growth and soil nutrient in red soil were studied. The results indicated that the economic benefit and the ratio of the outpu...The effects of the cropping system change for paddy field with double harvest rice on crops growth and soil nutrient in red soil were studied. The results indicated that the economic benefit and the ratio of the output to input were all increased in terms of the market price for the crops under various treatments. The greatest economic benefit was obtained in the treatment of paddy-upland rotation, and the corresponding economic benefit was increased by 34.7, 21.4, and 2.2% in comparison with that of control (rice-rice-astragali), pasture, and upland cropping treatments. The economic benefits in pasture and upland cultivation treatments were increased by I 1.0 and 31.8%, respectively, when compared with that of the control treatment (CK). The ratio of output to input in pasture, paddy-upland rotation, and upland cropping treatments was enhanced by 0.9, 0.6, and 0.3, respectively, in comparison with that of control. To grow pasture is beneficial for improving soil fertility since the contents of soil organic matter, total nitrogen, total phosphorus, and available phosphorus are all enhanced significantly. However, the concentrations of the soil available nitrogen, the total potassium, the available potassium were somewhat reduced in all the treatments, suggesting that increasing the input of nitrogen, particularly potassium, was necessary under the present fertilization level. Based on the conditions of fertility, climate, cultivation, and management of paddy field with double harvest rice in red soil regions, it is feasible to alter the cultivation system of paddy field with bad irrigation condition. In particular, cultivation systems such as pasture and paddy-upland rotation can be selected to extend because better economic benefit and improvement of soil fertility in the purpose region were obtained.展开更多
文摘【目的】研究绿肥、稻草不同还田模式对湘南地区水稻产量和土壤肥力的影响,为绿肥、稻草还田技术的推广和土壤培肥提供理论依据。【方法】2013—2018年在湘南红壤地区开展了6年田间小区定位试验,共设置6个处理,分别为冬闲+稻草不还田(CK)、冬种紫云英+稻草不还田(MV)、冬闲+早稻草全部还田(RS1)、冬闲+早、晚稻草全部还田(RS2)、冬种紫云英+早稻草全部还田(RS3)、冬种紫云英+早稻草全部还田+晚稻留高茬还田(RS4),各处理均施等量化肥。每季收获后测产,计算平均产量、产量变异系数和可持续指数。2017年晚稻收获后,取0—20 cm土层土壤样品,测定其养分含量和水稳性团聚体含量。【结果】与CK相比,除R S 1处理外,其余处理早稻、晚稻和周年平均产量均显著提高,增幅分别为6.6%~24.6%、11.9%~28.8%和9.0%~26.5%,以RS4处理增产效果最显著,其晚稻和周年平均产量均显著高于其他还田处理。与CK相比,除RS1处理早稻和MV处理晚稻外,其余处理早、晚稻产量变异系数均下降,且均以RS3处理最低;除晚稻MV处理外,其余处理早、晚稻产量可持续系数保持不变或提高,且早、晚稻均以RS3处理最高。试验开展5年后,各处理土壤有机质含量与初始土壤相比均明显提高,绿肥、稻草还田处理除RS1外均显著高于CK,RS4处理还显著高于RS1处理;各处理土壤全氮含量较2013年均有所降低,但绿肥、稻草还田处理较CK下降幅度小,MV和RS3处理全氮含量显著高于CK。土壤速效钾含量除MV和RS3处理低于CK外,其他处理均有不同程度的增加。粒径>0.25 mm水稳性团聚体以RS2处理最高,达68.9%。【结论】绿肥联合早稻草全部还田和晚稻留高茬还田措施可提高土壤有机质含量,稳定土壤氮素供应的长期效果显著,是湘南红壤地区水稻高产稳产和可持续发展相对较好的耕作制度,能够有效促进水稻增产,改善土壤理化性质,稳定氮素供应。
文摘种子被视为有益微生物的储存库和载体。与根际和叶际相比,种子相关细菌的研究还十分落后。本研究以长期绿肥植物与水稻轮作(稻-稻-紫云英,R-R-MV)和冬闲(稻-稻-冬闲,R-R-WF)两种种植模式的水稻种子为材料,分离种子内生细菌;在对菌株进行16S r RNA基因系统发育分析基础上,依据分离来源和分类地位选择代表菌株,进行溶磷、产IAA和铁载体等植物促生功能的检测。结果显示,绿肥轮作后水稻种子内生细菌的数量显著高于冬闲,群落组成也发生了明显变化。在属水平上,Pseudomonas和Xanthomonas是R-R-WF中的优势属,分别占28.57%和26.19%,而R-R-MV中Pantoea是优势属,占27.94%,其次是Pseudomonas(14.71%)和Paenibacillus(13.24%)。进行功能检测的31个菌株中,大多数分离株具有有益于植物的特性,表明种子内生细菌与植物的生长及健康密切相关。本研究不仅揭示了长期轮作条件可以改变种子的微生物组成,还获得了有益的微生物资源,将有助于种子内生菌作为生物接种剂在可持续农业中的研究和应用。
基金supported by Special Fund for Agro-scientific Research in the Public Interest(201103005)Ministry of Agriculture,China and the International Plant Nutrition Institute(IPNI,Hunan-14)
文摘Soil physical properties are important indicators of the potential for agricultural production.Our objective was to evaluate the effects of long-term inputs of green manures on physical properties of a reddish paddy soil(Fe-Typic Hapli-Stagnic Anthrosols) under a double cropping system.The common cropping pattern before the study was early-late rice-fallow(winter).The field treatments included rice-rice-fallow(R-R-WF),rice-rice-rape(R-R-RP),rice-rice-Chinese milk vetch(RR-MV),and rice-rice-ryegrass(R-R-RG).The rape,Chinese milk vetch and ryegrass were all incorporated as green manures 15 d before early rice transplanting during the following year.The soil bulk density in all green manure treatments was significantly reduced compared with the winter fallow treatment.Soil porosity with green manure applications was significantly higher than that under the winter fallow.The green manure treatments had higher 0.25-5 mm water stable aggregates and aggregates stabilities in the plow layer(0-15 cm depth) compared with the fallow treatment.The mean weight diameter(MWD) and normalized mean weight diameter(NMWD) of aggregates in the green manure treatment were larger than that with the winter fallow.Soil given green manure retained both a higher water holding capacity in the plow layer soil,and a larger volume of moisture at all matric potentials(-10,-33 and-100 kPa).We conclude that the management of double-rice fields in southern central China should be encouraged to use green manures along with chemical fertilizers to increase SOC content,improve soil physical properties and soil fertility.
基金supported by the earmarked fund for China Agriculture Research System (2013–2017)the Chinese Outstanding Talents Program in Agricultural Sciences
文摘Dissolved organic matter(DOM) plays important roles in soil biogeochemistry activity and nutrients transportation in soils, but studies regarding the long-term effects of green manures on the content and structure of DOM in red paddy soil have not been reported yet. A long-term green manure experiment established in 1982 was utilized to test the DOM contents in different treatments, and the spectral characteristics of DOM were investigated by using ultraviolet-visible(UV-Vis) spectrometry and Fourier transform infrared(FTIR) spectrometry. The experiment included four cropping systems: ricerice-milk vetch(RRV), rice-rice-rape(RRP), rice-rice-ryegrass(RRG) and rice-rice-winter fallow(RRF), among them, milk vetch, rape, and ryegrass are popular winter green manure species in southern China. The results showed that the content of dissolved organic carbon(DOC), which is widely used to estimate the concentration of DOM, was significantly promoted after the incorporation of green manures compared with the other sampling stages. The contents of aromatic groups and the degree of humification of DOM increased in RRV and RRP, suggesting more complex compositions of the soil DOM after long-term application of milk vetch and rape. The contents of phenol, alcohol and carboxylic acid group at the mature stage of early rice were significantly higher than those at the stage of after green manures turned over, especially for the RRV treatment. The absorption ratio of FTIR indicated that winter plantation of rape increased the aromatic-C/aliphatic-C ratio, and ryegrass increased the aromatic-C/carboxyl-C ratio. In conclusion, long-term planting of milk vetch and rape as green manures increased the degree of aromaticity, humification and average molecular weight of DOM, and made the DOM more stable in red paddy soil.
文摘The content of organic matter (OM), nitrogen (N), phosphorus (P), and potassium (K) in the three selected soils, including Calcaric Purpli-Orthic Primosols (purple sand-shale parent material), Dystric Turbi-Anthric Primosols (quaternary red clay parent material) and Typic Udi-Sandic Primosols (granite parent material) were studied under a long-term experiment by using crop straw and inorganic fertilizers at the Hunan Red Soil Experiment Station of Chinese Academy of Agricultural Sciences. The results showed that the contents of OM, N, P and K in the three selected soils increased after 23 years application of crop straw and inorganic fertilizers, but the contents increased much less when crop straw or inorganic fertilizers was applied alone. The nutrient contents in the three soils developed from granite changed more remarkably than those in the soil derived from quaternary red clay and purple sand-shale. It was also found that the contents of OM, N, and P increased slightly in the treatments without applying fertilizers or returning the crop straw to the root bed. Combined application of inorganic fertilizers and crop straw could remarkably increase the contents of OM, alkalihydrolyzable N and available K, the positive correlation between application of organic fertilizers and increase of OM in soil did not always happened, it provided evidence for the relation between appropriate C/N ratio and accumulation of OM in soil. The increase of nutrient content was influenced by the soil properties. By comparing the contents of nutrient in 0-20 cm depth in the three different soils, it was concluded that the most increases of OM, alkali-hydrolyzable N, and available P were observed in Typic Udi-Sandic Primosols with the average increase by 3.03, 27.38, and 21.73 mg kg^-1, respectively. The available K increased in Dystric Turbi-Anthric Primosols with the average increase by 25.82 mg kg^-1, while it decreased in Calcaric Purpli-Orthic Primosols and Typic Udi-Sandic Primosols. It was concluded that the application of inorganic fertilizer and crop straw was important to improve the soil fertility for all of three selected soils. The straw return to the field had played a significant role for enhancement of the soil quality in the study areas. The application of inorganic fertilizer combined with the straw return to the fields could remarkably improve the soil fertility.
基金supported by the Special Fund for Agroscientific Research in the Public Interest,China(201103005)the Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences(2013–2017)
文摘Dissimilatory Fe(Ⅲ) reduction is an important process in the geochemical cycle of iron in anoxic environment. As the main products of dissimilatory iron reduction, the Fe(Ⅱ) species accumulation could indicate the reduction ability. The effects of different green manures on Fe(Ⅲ) reduction in paddy soil were explored based on a 31-year rice-rice-winter green manure cropping experiment. Four treatments were involved, i.e., rice-rice-milk vetch (RRV), rice-rice-rape (RRP), rice-rice-ryegrass (RRG) and rice-rice-winter fallow (RRF). Soils were sampled at flowering stage of milk vetch and rape (S1), before transplantation (S2), at tillering (S3), jointing (S4), and mature (S5) stages of the early rice, and after the harvest of the late rice (S6). The contents of TFeHa (HCI-extractable total Fe), Fe(Ⅱ)HCI (HCI-extractable Fe(Ⅱ) species) and Fe(Ⅲ)HCI (HCI- extractable Fe(Ⅲ) species) were measured. The correlations among those Fe species with selected soil environmental factors and the dynamic characteristics of Fe(Ⅱ)HCI accumulation were investigated. The results showed that TFeHc~ in RRF was significantly higher than those in the green manure treatments at most of the sampling stages. Fe(II)Ha increased rapidly after the incorporation of green manures in all treatments and kept rising with the growth of early rice. Fe(Ⅱ)Ha in RRG was quite different from those in other treatments, i.e., it reached the highest at the S2 stage, then increased slowly and became the lowest one at the S4 and S5 stages. Fe(Ⅲ)Ha showed oppositely, and Fe(Ⅱ)HCI/Fe(Ⅲ)HCI performed similarly to Fe(Ⅱ)HCI The maximum accumulation potential of Fe(Ⅱ)HCI was significantly higher in RRF, while the highest maximum reaction rate of Fe(Ⅱ)Ha accumulation appeared in RRG. Significant correlations were found between the indexes of Fe(Ⅱ)HCI accumulation and soil pH, oxidation-reduction potential (Eh) and total organic acids, respectively. In together, we found that long-term application of green manures decreased the TFeHa in red paddy soils, but promoted the ability of Fe(lll) reduction, especially the ryegrass; Fe(Ⅱ)Ha increased along with the growth of rice and was affected by soil conditions and environmental factors, especially the water and redox ability.
基金supported by the Special Fund for Agro-scientific Research in the Public Interest of China(201103005)the Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences(2013–2017)
文摘On the basis of a long-term(30 years) field experiment that involved four rotation systems, rice-rice-winter fallow(RRF), rice-rice-ryegrass(RRG), rice-rice-rape(RRP), and rice-rice-milk vetch(RRV), this study described the effects of green manure on the microbial communities in the red paddy soils using 454 pyrosequencing for the 16 S r RNA gene. The Chao1 richness and non-parametric Shannon's index increased in all soil samples that received green manure treatments. The communities' structures with the green manure applications were significantly dissimilar from that under the winter fallow. Using Metastats tests, many genera in the RRG, RRP and RRV soils were significantly different from those in the RRF soil, including a number of genera that functioned in the nitrogen and sulfur cycles. Analyses of the genera with these functions revealed the shifts in microbial ecosystem functions after long-term green manuring. Changes in the microbial communities increased the ammonium supply and decreased the soil acidification in green-manure-amended soils. Together, these data suggested powerful effects of green manure on both the microbial communities and the biogeochemical cycle driven by the shifts in bacterial functional groups.
文摘Effects of two kinds of magnesium compound with fertilizer on Daylily (Hemerocallis citrina Baroni) growth, yield, and soil nutrients in red soil were studied. The results indicated that significant effects of magnesium applied to soils were observed in increasing Daylily (Hemerocallis citrina Baroni) yield, improving its growth, and strengthening its antivirus property as well as increasing the amount of exchangeable Mg, N, P, and K in red soil. In particular, the effects of magnesium compound fertilizer Ⅱ (MCF2) with higher Mg content were better than that of the others, which increased Daylily (Hemerocallis citrina Baroni) yield by 57.4, 32.8, and 14.5% compared to that of control treatment (CK), chemical fertilizer with nitrogen, phosphorus, potassium treatment (CF), and magnesium compound fertilizer Ⅰ treatment (MCF1) with lower Mg content. It increased soil Alkali N, available P, exchangeable K, and exchangeable Mg by 94.9, 46.5, 31.1, and 35.3%, respectively, compared with that of CK treatment. Therefore, the application of magnesium compound with fertilizer is an optimum method for improving red soil quality.
基金This study was financially supported by the earmarked fund for China Agriculture Research System(CARS-01-02A)the Natural Science Foundation of Hunan Province,China(2022NK2009)+2 种基金the Science and Technology Innovation Program of Hunan Province,China(2021RC2081)the Natural Science Foundation of Sichuan Province,China(2022NSFSC1059)the Development Program of Hunan Province,China(2021NK2029).
文摘Fertilization is an effective technique to improve soil fertility and increase crop yield.The long-term effects of different fertilizers on soil considerably vary.Over 38 consecutive years of different fertilization positioning experiments in a double cropping rice field of Qiyang Red Soil Experimental Station,seven different fertilization treatments including CK(no fertilization),NPK(nitrogen,phosphorus,and potassium fertilizer),M(cow manure),NPKM(nitrogen,phosphorus,and potassium with cow manure),NPM(nitrogen and phosphorus with cow manure),NKM(nitrogen and potassium with cow manure),and PKM(phosphorus and potassium with cow manure)were applied to study the effects on rice yield,soil fertility,and nutrient apparent balance in a paddy field.The results showed that the annual average yields of rice in NPKM,NPM,NKM,PKM,M,NPK and CK treatments ranged from 6214 to 11562 kg ha-1.Yields under long-term organic and inorganic treatments(NPKM,NPM,NKM and PKM)were 22.58,15.35,10.53 and 4.41%,respectively,greater than under the NPK treatment.Soil organic carbon(SOC),total nitrogen(TN),available nitrogen(AN)and available potassium(AK)concentration with long-term organic and inorganic treatment(NPKM,NPM,NKM and PKM)were significantly higher than in inorganic fertilizer(NPK)treatments.Soil total phosphorus(TP)and available phosphorus(AP)contentration with organic fertilizer combined with inorganic N and P fertilizer treatment(NPKM,NPM and PKM)were significantly higher than with inorganic fertilizer alone(NPK treatments).The average annual rice yield(11562 kg ha-1),SOC(20.88 g kg-1),TN(2.30 g kg-1),TP(0.95 g kg-1),TK(22.50 g kg-1)and AP(38.94 mg kg-1)concentrations were the highest in the NPKM treatment.The soil AN concentration(152.40 mg kg-1)and AK contentration(151.00 mg kg-1)were the highest in the NKM treatment.N and P application led to a surplus of nitrogen and phosphorus in the soil,but NPKM treatment effectively reduced the surplus compared with other treatments.Soils under all treatments were deficient in potassium.Correlation analysis showed that SOC,TN,AN,TP,and AP contentration was significantly correlated with rice yield;the correlation coefficients were 0.428,0.496,0.518,0.501,and 0.438,respectively.This study showed that the combined application of N,P,and K with cow manure had important effects on rice yield and soil fertility,but balanced application of N,P,and K with cow manure was required.
文摘The effects of the cropping system change for paddy field with double harvest rice on crops growth and soil nutrient in red soil were studied. The results indicated that the economic benefit and the ratio of the output to input were all increased in terms of the market price for the crops under various treatments. The greatest economic benefit was obtained in the treatment of paddy-upland rotation, and the corresponding economic benefit was increased by 34.7, 21.4, and 2.2% in comparison with that of control (rice-rice-astragali), pasture, and upland cropping treatments. The economic benefits in pasture and upland cultivation treatments were increased by I 1.0 and 31.8%, respectively, when compared with that of the control treatment (CK). The ratio of output to input in pasture, paddy-upland rotation, and upland cropping treatments was enhanced by 0.9, 0.6, and 0.3, respectively, in comparison with that of control. To grow pasture is beneficial for improving soil fertility since the contents of soil organic matter, total nitrogen, total phosphorus, and available phosphorus are all enhanced significantly. However, the concentrations of the soil available nitrogen, the total potassium, the available potassium were somewhat reduced in all the treatments, suggesting that increasing the input of nitrogen, particularly potassium, was necessary under the present fertilization level. Based on the conditions of fertility, climate, cultivation, and management of paddy field with double harvest rice in red soil regions, it is feasible to alter the cultivation system of paddy field with bad irrigation condition. In particular, cultivation systems such as pasture and paddy-upland rotation can be selected to extend because better economic benefit and improvement of soil fertility in the purpose region were obtained.