为实现肠炎沙门菌的快速定量分析,采用Tris辅助柠檬酸钠还原法制备新型胶体金并利用Au-S配位作用在纳米金球表面修饰一层4-巯基苯甲酸(4-MBA)拉曼信号分子,然后以此为标记材料建立肠炎沙门菌表面增强拉曼(surface-enhancement of Raman ...为实现肠炎沙门菌的快速定量分析,采用Tris辅助柠檬酸钠还原法制备新型胶体金并利用Au-S配位作用在纳米金球表面修饰一层4-巯基苯甲酸(4-MBA)拉曼信号分子,然后以此为标记材料建立肠炎沙门菌表面增强拉曼(surface-enhancement of Raman scattering,SERS)免疫层析检测技术,并对其灵敏度、特异性和重复性进行考察。结果表明:建立的SERS免疫层析技术对肠炎沙门菌的可视化最低检测限(LOD)为10^(6) CFU/mL,而通过拉曼信号检测时LOD为10^(5) CFU/mL,灵敏度比裸眼观察提高了10倍。在10^(5)~10^(8) CFU/mL的浓度范围可根据标准曲线y=30.23×exp(-x/-147)-215.83(R^(2)=0.995)对肠炎沙门菌进行定量分析。该SERS免疫层析技术与鼠伤寒沙门菌、鸡白痢沙门菌、甲型副伤寒沙门菌等多种血清型沙门菌和大肠杆菌、副溶血弧菌等常见病原无交叉反应,特异性良好。同一批次的不同试纸条和不同批次的试纸条对相同样品的检测结果基本一致,重复性良好。以粪便和鸡蛋壳为基质的加标试验回收率在94.7%~104.9%之间,回收率良好。结果表明,基于金纳米颗粒的肠炎沙门菌SERS免疫层析快速检测技术灵敏度较高,特异性强,重复性好,能弥补传统胶体金试纸条无法定量的不足,可用于肠炎沙门菌的快速定量检测。展开更多
Equipped with millions of sensors and smart meters in smart gird,a reliable and resilient wireless communication technology is badly needed.Mobile networks are among the major energy communication networks which contr...Equipped with millions of sensors and smart meters in smart gird,a reliable and resilient wireless communication technology is badly needed.Mobile networks are among the major energy communication networks which contribute to global energy consumption increase rapidly.As one of core technologies of smart grid employing mobile networks,Demand Response(DR) helps improving efficiency,reliability and security for electric power grid infrastructure.Security of DR events is one of the most important issues in DR.However,the security requirements of different DR events are dynamic for variousactual demands.To address this,an event-oriented dynamic security service mechanism is proposed for DR.Three kinds of security services including security access service,security communication service and security analysis service for DR event are composited dynamically by the fine-grained sub services.An experiment prototype of the network of State Grid Corporation of China(SGCC) is established.Experiment and evaluations shows the feasibility and effectiveness of the proposed scheme in smart grid employing mobile network.展开更多
文摘目的观察培美曲塞联合顺铂治疗非小细胞肺癌(non-small cell lung cancer,NSCLC)患者的效果及对其生存质量的影响。方法选取梅州市人民医院收治的56例NSCLC患者作为研究对象,依据随机数字表法分为研究组(n=33)和对照组(n=23)。对照组采用吉西他滨联合顺铂治疗,研究组采用培美曲塞联合顺铂治疗,比较两组近期疗效、不良反应发生率及治疗前后生存质量。结果治疗后,研究组疾病控制率(disease control rate,DCR)及肿瘤患者生活质量(quality of life,QOL)评分均明显高于对照组,差异有统计学意义(P<0.05);研究组不良反应发生率为3.03%,明显低于对照组的21.74%,差异有统计学意义(P<0.05)。结论培美曲塞联合顺铂治疗非小细胞肺癌患者可提高近期疗效及生命质量,降低不良反应发生率的效果优于吉西他滨联合顺铂治疗效果。
基金supported by National Natural Science Foundation of China(Grant No. 61401273 and 61431008)Doctoral Scientific Fund Project of the Ministry of Education of China(No.20130073130006)JSPS KAKENHI Grant Number 15K15976,26730056,JSPS A3 Foresight Program
文摘Equipped with millions of sensors and smart meters in smart gird,a reliable and resilient wireless communication technology is badly needed.Mobile networks are among the major energy communication networks which contribute to global energy consumption increase rapidly.As one of core technologies of smart grid employing mobile networks,Demand Response(DR) helps improving efficiency,reliability and security for electric power grid infrastructure.Security of DR events is one of the most important issues in DR.However,the security requirements of different DR events are dynamic for variousactual demands.To address this,an event-oriented dynamic security service mechanism is proposed for DR.Three kinds of security services including security access service,security communication service and security analysis service for DR event are composited dynamically by the fine-grained sub services.An experiment prototype of the network of State Grid Corporation of China(SGCC) is established.Experiment and evaluations shows the feasibility and effectiveness of the proposed scheme in smart grid employing mobile network.
