With the rapid development of the aviation industry,the development of intelligent manufacturing equipment represented by composite robots has been paid close attention by the aviation industry.Based on the analysis o...With the rapid development of the aviation industry,the development of intelligent manufacturing equipment represented by composite robots has been paid close attention by the aviation industry.Based on the analysis of the background and main structure function of composite robots,this paper focuses on the analysis of key technologies such as composite robot hardware design,visual sensing and planning system,integrated control of‘hands,feet,and eyes',multi-robot collaborative operation,and safety.The typical applications of composite robots in aviation intelligent manufacturing such as automatic drilling and connection of aircraft,aircraft surface spraying and finishing,parts handling,aircraft measurement,and inspection are presented.The development trends such as standardization of composite robots,integration of‘5G+cloud computing+AI',and fusion of intelligent sensors are proposed.展开更多
The phosphoenolpyruvate(PEP):carbohydrate phosphotransferase system(PTS)is widely used by bacteria to take up sugars or sugar derivatives;.PTS is usually composed of a histidine protein(HPr),enzyme I(EI),and several e...The phosphoenolpyruvate(PEP):carbohydrate phosphotransferase system(PTS)is widely used by bacteria to take up sugars or sugar derivatives;.PTS is usually composed of a histidine protein(HPr),enzyme I(EI),and several enzyme II(EII)proteins,such as EIIA,EIIB,EIIC,and EIID[1].展开更多
Objective This study aimed to investigate whether the VCA0560 gene acts as an active diguanylate cyclase(DGC)in Vibrio cholerae and how its transcription is regulated by Fur and Hap R.Methods The roles of VCA0560 was ...Objective This study aimed to investigate whether the VCA0560 gene acts as an active diguanylate cyclase(DGC)in Vibrio cholerae and how its transcription is regulated by Fur and Hap R.Methods The roles of VCA0560 was investigated by utilizing various phenotypic assays,including colony morphological characterization,crystal violet staining,Cyclic di-GMP(c-di-GMP)quantification,and swimming motility assay.The regulation of the VCA0560 gene by Fur and Hap R was analyzed by luminescence assay,electrophoretic mobility shift assay,and DNase I footprinting.Results VCA0560 gene mutation did not affect biofilm formation,motility,and c-di-GMP synthesis in V.cholerae,and its overexpression remarkably enhanced biofilm formation and intracellular c-di-GMP level but reduced motility capacity.The transcription of the VCA0560 gene was directly repressed by Fur and the master quorum sensing regulator Hap R.Conclusion Overexpressed VCA0560 functions as an active DGC in V.cholerae,and its transcription is repressed by Fur and Hap R.展开更多
Objective This study is to verify the use of rich BHI medium to substitute synthetic media for gene regulation studies in Yersinia pestis. Methods The transcriptional regulation of rovA by PhoP or via temperature upsh...Objective This study is to verify the use of rich BHI medium to substitute synthetic media for gene regulation studies in Yersinia pestis. Methods The transcriptional regulation of rovA by PhoP or via temperature upshift, and that of pla by CRP were investigated when Y. pestis was cultured in BHI. After cultivation under 26 ~C, and with temperature shifting from 26 to 37 ~C, the wild-type （WT） strain or its phoP or crp null mutant （AphoP or Acrp, respectively） was subject to RNA isolation, and then the promoter activity of rovA or plo in the above strains was detected by the primer extension assay. The rovA promoter-proximal region was cloned into the pRW50 containing a promoterless lacZ gene. The recombinant LacZ reporter plasmid was transformed into WT and AphoP to measure the promoter activity of rovA in these two strains with the ^-Galactosidase enzyme assay system. Results When Y. pestis was cultured in BHI, the transcription of rovA was inhibited by PhoP and upon temperature upshift while that ofpla was stimulated by CRP. Conclusion The rich BHI medium without the need for modification to be introduced into the relevant stimulating conditions （which are essential to triggering relevant gene regulatory cascades）, can be used in lieu of synthetic TMH media to cultivate Y. pestis for gene regulation studies.展开更多
基金the National Key Research and Development Program of China(No.2022YFB4700400)。
文摘With the rapid development of the aviation industry,the development of intelligent manufacturing equipment represented by composite robots has been paid close attention by the aviation industry.Based on the analysis of the background and main structure function of composite robots,this paper focuses on the analysis of key technologies such as composite robot hardware design,visual sensing and planning system,integrated control of‘hands,feet,and eyes',multi-robot collaborative operation,and safety.The typical applications of composite robots in aviation intelligent manufacturing such as automatic drilling and connection of aircraft,aircraft surface spraying and finishing,parts handling,aircraft measurement,and inspection are presented.The development trends such as standardization of composite robots,integration of‘5G+cloud computing+AI',and fusion of intelligent sensors are proposed.
基金supported by grants from the National Natural Science Foundation of China[Grant No.81471917]the Science Foundation for the State Key Laboratory for Infectious Disease Prevention and Control of China[Grant No.2015SKLID509]。
文摘The phosphoenolpyruvate(PEP):carbohydrate phosphotransferase system(PTS)is widely used by bacteria to take up sugars or sugar derivatives;.PTS is usually composed of a histidine protein(HPr),enzyme I(EI),and several enzyme II(EII)proteins,such as EIIA,EIIB,EIIC,and EIID[1].
基金supported by grants from the National Natural Science Foundation of China[Grant No.81471917]the National Basic Research Priorities Program[Grant 2015CB554201]the Science Foundation for the State Key Laboratory for Infectious Disease Prevention and Control of China[Grant No.2015SKLID509]
文摘Objective This study aimed to investigate whether the VCA0560 gene acts as an active diguanylate cyclase(DGC)in Vibrio cholerae and how its transcription is regulated by Fur and Hap R.Methods The roles of VCA0560 was investigated by utilizing various phenotypic assays,including colony morphological characterization,crystal violet staining,Cyclic di-GMP(c-di-GMP)quantification,and swimming motility assay.The regulation of the VCA0560 gene by Fur and Hap R was analyzed by luminescence assay,electrophoretic mobility shift assay,and DNase I footprinting.Results VCA0560 gene mutation did not affect biofilm formation,motility,and c-di-GMP synthesis in V.cholerae,and its overexpression remarkably enhanced biofilm formation and intracellular c-di-GMP level but reduced motility capacity.The transcription of the VCA0560 gene was directly repressed by Fur and the master quorum sensing regulator Hap R.Conclusion Overexpressed VCA0560 functions as an active DGC in V.cholerae,and its transcription is repressed by Fur and Hap R.
基金supported by the National Natural Science Foundation of China (30930001 and 30900823)
文摘Objective This study is to verify the use of rich BHI medium to substitute synthetic media for gene regulation studies in Yersinia pestis. Methods The transcriptional regulation of rovA by PhoP or via temperature upshift, and that of pla by CRP were investigated when Y. pestis was cultured in BHI. After cultivation under 26 ~C, and with temperature shifting from 26 to 37 ~C, the wild-type （WT） strain or its phoP or crp null mutant （AphoP or Acrp, respectively） was subject to RNA isolation, and then the promoter activity of rovA or plo in the above strains was detected by the primer extension assay. The rovA promoter-proximal region was cloned into the pRW50 containing a promoterless lacZ gene. The recombinant LacZ reporter plasmid was transformed into WT and AphoP to measure the promoter activity of rovA in these two strains with the ^-Galactosidase enzyme assay system. Results When Y. pestis was cultured in BHI, the transcription of rovA was inhibited by PhoP and upon temperature upshift while that ofpla was stimulated by CRP. Conclusion The rich BHI medium without the need for modification to be introduced into the relevant stimulating conditions （which are essential to triggering relevant gene regulatory cascades）, can be used in lieu of synthetic TMH media to cultivate Y. pestis for gene regulation studies.
