Genome-wide and candidate gene association studies identify BnPAP17 as conferring the utilization of organic phosphorus in oilseed rape

Phosphorus(P)is essential for living plants,and P deficiency is one of the key factors limiting the yield in rapeseed production worldwide.As the most important organ for plants,root morphology traits(RMTs)play a key role in P absorption.To investigate the genetic variability of RMT under low P availability,we dissected the genetic structure of RMTs by genome-wide association studies(GWAS),linkage mapping and candidate gene association studies(CGAS).A total of 52 suggestive loci were associated with RMTs under P stress conditions in 405 oilseed rape accessions.The purple acid phosphatase gene BnPAP17 was found to control the lateral root number(LRN)and root dry weight(RDW)under low P stress.The expression of BnPAP17 was increased in shoot tissue in P-efficient cultivars compared to root tissue and P-inefficient cultivars in response to low P stress.Moreover,the haplotype of BnPAP17^(Hap3)was detected for the selective breeding of P efficiency in oilseed rape.Over-expression of the BnPAP17^(Hap3)could promote the shoot and root growth with enhanced tolerance to low P stress and organic phosphorus(Po)utilization in oilseed rape.Collectively,these findings increase our understanding of the mechanisms underlying BnPAP17-mediated low P stress tolerance in oilseed rape.

Study on Electromyographic Characteristics of Neck Muscles in College Students with Chronic Neck Pain

Objective:To provide new insights for the evaluation and diagnosis of chronic neck pain(CNP).Methods:22 patients with CNP and 22 healthy individuals were recruited from South China Normal University,who were all college students.The subjects'neck extensor muscle strength in the head neutral position,the natural anteversion position,and the maximum forward flexion position were measured by an isometric muscle strength tester respectively.The neck extensor strength of CNP patients and healthy subjects were compared.Results:In the neutral position,the maximum isometric muscle strength of neck extensor muscles was 12.31 kg for CNP patients and 15.16 kg for healthy individuals,resulting in a ratio of 81%strength in patients compared to healthy subjects.This difference was highly significant(P<0.000).In the natural anteversion position,the respective values were 12.6 kg for CNP patients and 15.05 kg for healthy individuals,with a ratio of 83%,and a significant difference between groups(P<0.001).In the maximum forward flexion position of the head,the values were 13.36 kg for CNP patients and 16.15 kg for healthy individuals,with a ratio of 82%,and a highly significant difference(P<0.000).Conclusion:The neck extensor muscle strength levels in college students with CNP were significantly lower compared to healthy college students across all measured positions.

Micro RNA-548a-5p promotes proliferation and inhibits apoptosis in hepatocellular carcinoma cells by targeting Tg737

AIM: To investigate whether Tg737 is regulated by micro RNA-548a-5p(mi R-548a-5p), and correlates with hepatocellular carcinoma(HCC) cell proliferation and apoptosis.METHODS: Assays of loss of function of Tg737 were performed by the colony formation assay, CCK assay and cell cycle assay in HCC cell lines. The interaction between mi R-548a-5p and its downstream target, Tg737, was evaluated by a dual-luciferase reporter assay and quantitative real-time polymerase chain reaction. Tg737 was then up-regulated in HCC cells to evaluate its effect on mi R-548a-5p regulation. Hep G2 cells stably overexpressing mi R-548a-5p or mi R-control were also subcutaneously inoculated into nude mice to evaluate the effect of mi R-548a-5p up-regulation on in vivo tumor growth. As the final step, the effect of mi R-548a-5p on the apoptosis induced by cisplatin was evaluated by flow cytometry.RESULTS: Down-regulation of Tg737, which is a target gene of mi R-548a-5p, accelerated HCC cell proliferation, and mi R-548a-5p promoted HCC cell proliferation in vitro and in vivo. Like the downregulation of Tg737, overexpression of mi R-548a-5p in HCC cell lines promoted cell proliferation, increased colony forming ability and hampered cell apoptosis. In addition, mi R-548a-5p overexpression increased HCC cell growth in vivo. Mi R-548a-5p downregulated Tg737 expression through direct contact with its 3' untranslated region(UTR), and mi R-548a-5p expression was negatively correlated with Tg737 levels in HCC specimens. Restoring Tg737(without the 3'UTR) significantly hampered mi R-548a-5p induced cell proliferation, and rescued the mi R-548a-5p induced cell proliferation inhibition and apoptosis induced by cisplatin.CONCLUSION: Mi R-548a-5p negatively regulates the tumor inhibitor gene Tg737 and promotes tumorigenesis in vitro and in vivo, indicating its potential as a novel therapeutic target for HCC.

不同浓度理气化痰活血方含药血清对大鼠心脏微血管内皮细胞活力的影响

目的探讨理气化痰活血方(LQHTHX)含药血清对缺氧/复氧诱导原代大鼠心脏微血管内皮细胞(CMECs)活力的影响。方法采用水煎煮法制备LQHTHX提取液并浓缩至高(2.7 g生药/mL)、中(1.35g生药/mL)、低(0.675 g生药/mL)3个剂量,按照10 mL/kg连续灌胃大鼠3d,在末次给药后0.5,1,1.5,2,2.5h采取血液样本分离血清,配置成5%、10%、15%以及20%的含药血清细胞培养基。提取新生大鼠原代CMECs并进行免疫荧光鉴定,体外构建细胞缺氧模型,分别加入不同浓度含药血清,采用CCK8法检测各组细胞活力,比较不同浓度含药血清处理后的细胞活力差异。结果成功提取了大鼠原代CMECs并构建了细胞缺氧模型。与模型组相比,阳性药物尼可地尔(200μmol/L)、缬沙坦(50μmol/L)以及中、高剂量组中不同浓度含药血清均能提高CMECs活力(P<0.01),其中以高剂量组中10%、15%LQHTHX含药血清组效果最好。结论LQHTHX可有效改善缺氧诱导的CMECs活力下降,从而发挥保护心脏微血管内皮功能的作用。

中医药治疗老年单纯收缩期高血压的临床研究结局指标分析

[目的]梳理中医药治疗老年单纯收缩期高血压(ISH)的随机对照试验(RCT)的评价指标,构建中医药治疗老年ISH临床试验核心指标集。[方法]检索PubMed、CoChrane library、Embase、中国知网数据库、万方数据库、维普数据库中应用中药联合长效钙拮抗剂(CCB)或肾素-血管紧张素-醛固酮系统阻断剂(ACEI或ARB)干预老年ISH的随机对照临床研究,检索时限均为建库至2019年12月31日。[结果]纳入38篇RCT,共计2848例患者,涉及396个指标,频次排列前16位的指标依次为:诊室收缩压(7.04%)、降压有效率(6.31%)、中医证候疗效(6.31%)、诊室舒张压(5.56%)、肝功能(4.29%)、肾功能(4.29%)、血常规(4.04%)、尿常规(3.79%)、不良反应发生率(3.54%)、夜间平均收缩压(3.28%)、24小时平均收缩压(3.03%)、日间平均收缩压(3.03%)、中医证候积分(3.03%)、诊室脉压差(2.78%)、日间平均舒张压(2.78%)及夜间平均舒张压(2.78%)。[结论]中医药治疗老年ISH的指标选择存在较多的问题,如差异性较大、指标测量时点差异大、缺乏中医药特色等,需加快研制老年ISH;核心指标集。

耳穴贴压联合悬灸法对心力衰竭气虚血瘀证患者生活质量的影响

[目的]评价耳穴贴压联合悬灸法对心力衰竭气虚血瘀证患者生活质量的影响。[方法]采用随机对照研究设计,将80例心力衰竭气虚血瘀证患者随机分为治疗组和对照组各40例,两组均根据相关规范给予心力衰竭常规护理,治疗组在心力衰竭常规护理的基础上使用耳穴贴压联合悬灸法,两组疗程均为10 d,评价时点为治疗前及治疗后10 d,以Memorial心力衰竭症状评估量表(MSAS-HF)评分(心力衰竭相关症状总分及各维度评分)、中医证候积分、明尼苏达心力衰竭生存质量量表(MLHFQ)评分为临床疗效评价指标。[结果]治疗后两组患者MSAS-HF评分、中医证候积分及MLHFQ评分均较治疗前降低,治疗组优于对照组,差异具有统计学意义(P<0.05)。[结论]在常规护理的基础上,加用耳穴贴压联合悬灸法治疗心力衰竭气虚血瘀证患者可进一步减轻患者临床症状,提高生活质量。

小儿宣肺止咳颗粒对大鼠的长期毒性反应

目的观察小儿宣肺止咳颗粒对大鼠的长期毒性反应,为其临床应用提供参考。方法大鼠120只,随机分为正常对照组和小儿宣肺止咳颗粒小、中、大剂量组(3.00,7.80,20.25 g·kg^(-1)·d^(-1)),每组30只。对大鼠连续灌胃给药1个月,正常对照组给予等体积纯化水。观察大鼠一般状况、体质量、摄食量、血液学、血液生化学、眼科、尿液、脏器系数及病理组织学变化。结果小儿宣肺止咳颗粒对大鼠血液学、尿液、眼科、脏器系数均无明显影响;对大鼠体质量有一定的抑制作用,尤以大剂量对体质量的影响较大,并且对雄性大鼠的影响较雌性大鼠明显;大剂量对雄性大鼠摄食量有一定的抑制作用,可引起肝脏轻度损伤,停药4周后药物的毒性反应可逆,未见药物引起的其他延迟性毒性反应。结论小儿宣肺止咳颗粒7.80 g·kg^(-1)为大鼠无毒性反应剂量。

多元化教学模式在中医心血管内科住院医师规范化培训中的实践

心血管疾病严重威胁人类健康,是我国居民主要死亡原因之一。现阶段我国中医心血管内科住院医师规范化培训过程中,心血管疾病教学尤其是中医心病学的临床教学模式形式多样,且均取得了一定的教学效果。临床教学实践显示,根据教学大纲要求制定教学目标和计划,在以教师为主导的传统教学模式基础上,联合应用团队合作学习(TBL)教学法、基于问题的学习(PBL)教学法、中医特色教学培养等教学模式,注重理论与实践结合,可提高医师的综合能力,有助于培养出具有独立发现和解决问题能力的高素质中医心血管内科住院医师。

芪苈强心胶囊对射血分数保留心力衰竭患者生活质量影响的Meta分析

[目的]系统评价芪苈强心胶囊对射血分数保留心力衰竭(HFpEF)患者生活质量影响的有效性及安全性,并对证据质量等级进行评价。[方法]计算机检索中国知网数据库(CNKI)、万方数据库(WanFang Data)、维普资讯中文科技期刊数据库(VIP)、中国生物医学文献数据库(SinoMed)、PubMed、Embase和Cochrane Library共7个中英文数据库,收集芪苈强心胶囊治疗HFpEF的临床随机对照试验(RCT),检索时限均从建库至2020年8月1日。采用Cochrance偏倚风险评估工具对纳入研究进行方法学质量评价,利用RevMan 5.3软件进行Meta分析及发表偏倚评估。最后根据GRADE标准对证据级别进行评价。[结果]共纳入26项RCT,2221例HFpEF患者。Meta分析结果显示芪苈强心胶囊组在提高HFpEF患者临床综合疗效[RR=1.24,95%CI(1.18,1.31),P<0.00001]、改善中医证候疗效[RR=1.18,95%CI(1.05,1.32),P=0.005]、降低明尼苏达心力衰竭生活质量量表评分[WMD=-8.70,95%CI(-10.24,-7.17),P<0.0001]、增加6 min步行距离[WMD=58.78,95%CI(38.79,78.76),P<0.00001]、提高左室射血分数[WMD=4.06,95%CI(3.12,5.00),P<0.00001]、降低舒张早期二尖瓣瓣口血流峰值速度/二尖瓣环舒张早期运动速度[WMD=-2.70,95%CI(-3.52,-1.88),P<0.00001]、降低左心房容积指数[WMD=-5.68,95%CI(-6.83,-4.53),P<0.00001]方面疗效显著。不良反应发生率方面,两组比较无统计学差异(P>0.05)。基于GRADE标准,“降低明尼苏达心力衰竭生活质量量表评分”“降低舒张早期二尖瓣瓣口血流峰值速度/二尖瓣环舒张早期运动速度”“降低左心房容积指数”“不增加不良反应发生率”被评为中等质量证据;“提高临床综合疗效”“改善中医证候疗效”“增加6 min步行距离”“提高左室射血分数”被评为低质量证据。[结论]芪苈强心胶囊联合西医常规治疗在改善HFpEF患者的临床症状、提高运动耐量及心脏舒张功能方面确有疗效,且未见不良反应增加。鉴于GRADE评价证据质量为中等或低质量,对于研究结果的应用还应谨慎。

六味地黄丸在心血管疾病的应用价值及网络药理学多靶点效应价值研究进展

为明确六味地黄丸在心血管疾病(CVD)的应用价值及其复杂性化学组成成分、多靶点、多通路药物作用机制,本研究围绕其应用现状、网络药理多靶点效应价值进展进行整理分析,发现六味地黄丸在高血压、冠心病等疾病治疗中均有良好临床效果,其在心血管领域应用价值较高;网络药理学技术可实现中药治疗系统性、整体性评价,其指出六味地黄丸可通过调节脂多糖代谢、炎症反应等多个靶点,参与肿瘤坏死因子(TNF)、缺氧诱导因子-1(HIF-1)等多信号通路治疗心血管疾病,进而为临床治疗提供可靠指导。

绝缘油种类对油纸绝缘直流空间电荷特性的影响

在换流变压器中,油纸绝缘结构主要由矿物油和绝缘纸组成,而植物油应用较少。为了研究不同种类绝缘油对油纸绝缘空间电荷特性的影响,制备了KI 50X矿物油和FR3植物油与Kraft绝缘纸组合的两种油纸绝缘结构。通过电声脉冲法测量了两种油纸试样在直流电场下加压和去压时的空间电荷波形分布,得到了矿物油浸纸与植物油浸纸的空间电荷积聚特性和消散特性。结果表明:在加压阶段,FR3试样空间电荷积聚量更多;在去压阶段,FR3试样空间电荷残余量更少。两种油纸试样内部陷阱均以浅陷阱为主,FR3试样拥有更多的较浅能级陷阱,能够使空间电荷更快消散;KI 50X试样拥有更多的较深能级陷阱,有更多的残余空间电荷。从空间电荷特性的角度,植物油浸纸可以应用于换流变压器油纸绝缘结构。

冠状动脉微循环障碍的中医药治疗研究近况

近年来,溶栓及冠状动脉介入技术的发展使冠状动脉狭窄或阻塞问题得到了很大程度的改善,而冠状动脉微循环障碍(coronary microcirculation dysfunction,CMD)问题则日渐突出,越来越受到医学界的关注。CMD病因及发病机制复杂,现代医学尚缺乏针对CMD的治疗策略,中医辨证治疗显示出一定的临床疗效。该文重点就近年来中医药治疗CMD的研究情况进行阐述,旨在为CMD的临床诊治提供参考。

一种基于最短路径的视频摘要方法

视频摘要以简短的内容概括原始视频,是对原始视频的浓缩或者总结。传统的视频摘要方法有基于聚类的方法,通过视频帧的局部或全局特征将视频帧分成多个有代表性的集群,在每个集群中提取关键帧,最后去除冗余生成摘要;也有基于图模型的方法,将视频帧作为顶点,连接顶点的边表示帧间的关系,通过图的模块化信息提取关键帧。文章将聚类方法和图模型的方法结合并优化,提出了一种新的基于最短路径算法的视频摘要方法(shortest path for video summary,SPVS)。SPVS方法将视频摘要问题转化为最短路径求解问题,路径上的帧即为关键帧,在数据集上的大量主客观实验结果都表明其具有良好的性能。

基于网络药理学的理气化痰活血方治疗心脏X综合征分子机制研究

[目的]运用网络药理学分析理气化痰活血方治疗心脏X综合征的分子机制。[方法]从TCMSP平台获取理气化痰活血方12味中药的化学成分和靶点,利用TTD、Drugbank、DisGeNET数据库筛选心脏X综合征的靶点,将心脏X综合征靶点与药物靶点进行Venn分析,筛选两者共同靶点,利用Cytoscape3.5.1软件构建化合物-靶点、化合物-基因靶点-通路-疾病网络,应用string平台构建蛋白-蛋白相互作用网络。最后,通过DAVID(6.8)在线分析数据工具对共同靶点进行GO富集分析和KEGG通路富集分析。[结果]共筛选出理气化痰活血方165种化学成分及与治疗心脏X综合征相关的关键靶点35个,靶点主要涉及炎症反应、细胞增殖与凋亡、物质代谢、激素分泌等过程,可能通过调节TNF、MAPK、IL-17、TLRs、NLRs、HIF-1、TCR、PI3K-Akt等信号通路治疗心脏X综合征。[结论]理气化痰活血方主要以抗炎、抗氧化、抗心肌缺血、保护血管内皮功能、调节代谢等多成分、多靶点和多途径协同作用治疗心脏X综合征,为其临床应用及进一步研究其药效作用机制奠定基础。

单纯疱疹病毒Ⅰ型在三叉神经节潜伏复发机制的研究进展

单纯疱疹病毒Ⅰ型(HSV-1)感染引起的单纯疱疹病毒性角膜炎(HSK)是全球性的致盲性眼病。病毒在眼表发生原发感染后,转运并终生潜伏在三叉神经节内,反复复发引起角膜病变。在对HSV潜伏机制方面的研究过程中,人们逐渐认识到病毒自身和宿主反应两方面共同调控了病毒的潜伏过程。近年来,嗜神经病毒的侵袭、免疫、潜伏复发等各方面的分子机制研究取得大量研究成果,为HSK的防治提供了新思路。本文将主要对HSV-1角膜初发感染后在三叉神经节内潜伏分子机制方面的研究进展进行介绍,并对目前HSK的基础研究和临床治疗存在的问题进行探讨。

Herpes simplex virus-1 infection or Simian virus 40-mediated immortalization of corneal cells causes permanent translocation of NLRP3 to the nuclei

AIM: To investigate into the potential involvement of pyrin containing 3 gene(NLRP3), a member of the nucleotide-binding oligomerization domain-like receptors with cytosolic pattern recognition, in the host defense of corneas against viruses.METHODS: The herpes viral keratitis model was utilized in BALB/c mice with inoculation of herpes simplex virus-1(HSV-1). Corneal tissues removed during therapy of patients with viral keratitis as well as a Simian vacuolating virus 40(SV40)-immortalized human corneal epithelial cell line were also examined.Immunohistochemistry was used to detect NLRP3 in these subjects, focusing on their distribution in tissue or cells. Western blot was used to measure the level of NLRP3 and another two related molecules in NLPR3 inflammasome, namely caspase-1 and IL-1β.RESULTS: The NLRP3 activation induced by HSV-1infection in corneas was accompanied with redistribution of NLRP3 from the cytoplasm to the nucleus in both murine and human corneal epithelial cells. Furthermore,in the SV40-immortalized human corneal epithelial cells,NLRP3 was exclusively located in the nucleus, and treatment of the cells with high concentration of extracellular potassium(known as an inhibitor of NLRP3activation) effectively drove NLRP3 back to the cytoplasm as reflected by both immunohistochemistry and Western blot.· CONCLUSION: It is proposed that herpes virus infection activates and causes redistribution of NLRP3 to nuclei. Whether this NLRP3 translocation occurs with other viral infections and in other cell types merit further study.

A new veno-venous bypass type for ex-vivo liver resection in dogs

Ex-vivo liver resection is a procedure in which the liver is completely removed, perfused and after bench surgery, the liver is autotransplanted to the original site. Ex vivo liver resection is an important treatment for unresectable liver tumors. This surgical procedure requires long operation time, during which blood flow must be carefully maintained to avoid venous congestion. An effective veno-venous bypass (VVB) may meet this requirement. The present study was to test our new designed VVB device which comprised one heparinized polyvinylchloride tube and three magnetic rings The efficacy of this device was tested in five dogs. A VVB was established in 6-10 minutes. There was no leakage during the procedure. Hemodynamics was stable at anhepatic phase, which indicated that the bypass was successful. This newly-developed VVB device maintained circulation stability during ex-vivo liver resection in our dog model and thus, this VVB device significantly shortened the operation time.

类风湿关节炎患者外周血单个核细胞TNIP1的表达水平与主要临床指标的相关性分析

目的探讨类风湿关节炎(rheumatoid arthritis,RA)患者外周血中CD14^(+)单核细胞、CD4^(+)T细胞、CD8^(+)T细胞、调节性T细胞(regulator T cell,Treg)中肿瘤坏死因子诱导蛋白3相互作用蛋白1(tumor necrosis factor alpha-induced protein 3-interacting protein 1,TNIP1)的表达与类风湿因子(rheumatoid factor,RF)、抗环瓜氨酸肽(cyclic citrulline peptide,CCP)抗体等主要临床指标的相关性。方法选取桂林医学院附属医院62例RA患者和39例健康体检者(health donor,HD),RA患者住院期间(HD于同期)抽取外周静脉血。采用流式细胞术检测HD和RA患者外周血中CD14^(+)单核细胞、CD4^(+)T细胞、CD8^(+)T细胞、CD4^(+)Foxp3^(+)Treg细胞TNIP1表达水平并转换为Z-score,并对比HD和RA患者TNIP1 Z-score;分析RA患者TNIP1 Z-score与RF、抗CCP抗体等主要临床指标的相关性。结果RA患者外周血中CD14^(+)单核细胞TNIP1表达水平与健康体检者差异无统计学意义,CD4^(+)T细胞、CD8^(+)T细胞、CD4^(+)Foxp3^(+)Treg细胞TNIP1表达水平显著高于健康体检者(P<0.05);RA患者CD4^(+)T细胞、CD8^(+)T细胞、CD4^(+)Foxp3^(+)Treg细胞TNIP1表达水平与RF呈正相关(P<0.01、P<0.01、P<0.05);RA患者CD4^(+)T细胞、CD8^(+)T细胞TNIP1 Z-score与RF呈正向线性相关(P<0.01)。结论RA患者TNIP1的高表达可能增强CD4^(+)T细胞、CD8^(+)T细胞促炎的能力,而抑制CD4^(+)Foxp3^(+)Treg细胞抑炎的能力,RA患者TNIP1高表达可能促进病程的发展。

Superior performance porous carbon nitride nanosheets for helium separation from natural gas:Insights from MD and DFT simulations

Increasing helium(He)demand in fundamental research,medical,and industrial processes necessitates efficient He purification from natural gas.However,most theoretically available membranes focus on the separation of two or three kinds of gas molecules with He and the underlying separation mechanism is not yet well understood.Using molecular dynamic(MD)and first-principle density function theory(DFT)simulations,we systematically demonstrated a novel porous carbon nitride membrane(g-C_(9)N_(7))with superior performance for He separation from natural gas.The structure of g-C_(9)N_(7) monolayer was optimized first,and the calculated cohesive energy confirmed its structural stability.Increasing temperature from 200 to 500 K,the g-C_(9)N_(7)membrane revealed high He permeability,as high as 1.48×10^(7) GPU(gas permeation unit,1 GPU=3.35×10^(-10) mol·s^(-1)·Pa^(-1)·m^(-2))at 298 K,and also exhibited high selectivity for He over other gases(Ar,N_(2),CO_(2),CH_(4),and H_(2)S).Then,the selectivity of He over Ne was found to decrease with increasing the total number of He and Ne molecules,and to increase with increasing He to Ne ratio.More interestingly,a tunable He separation performance can be achieved by introducing strain during membrane separation.Under the condition of 7.5%compressive strain,the g-C_(9)(N_(7)(membrane reached the highest He over Ne selectivity of 9.41×10^(2).It can be attributed to the low energy barrier for He,but increased energy barrier for other gases passing through the membrane,which was subject to a compressive strain.These results offer important insights into He purification using g-C_(9)N_(7)membrane and opened a promising avenue for the screening of industrial grade gas separation with strain engineering.

Skull metastasis revealing a papillary thyroid carcinoma

Although thyroid carcinoma is a relatively common form of malignancy,metastatic spread to the skull is rare.Here,we report a case of papillary thyroid carcinoma with frontal and parietal metastasis.A 61-year-old Chinese woman presented with a one year history of a growing mass on the center of the frontal and parietal bone,initially thought to be meningioma.Biopsy of the skull base mass after intracalvarium excision,indicated a tumor of thyroid origin.One month later the patient underwent a total thyroidectomy.Pathological examination confirmed a diagnosis of papillary thyroid carcinoma with frontal and parietal bone metastasis.Based on this experience,the key to successful management of the skull metastasis of thyroid carcinoma is prompt diagnosis and appropriate treatment.Skull metastasis should be considered at the outset of the clinical course of papillary thyroid cancer.To facilitate this,patients should be meticulously investigated by a multidisciplinary team to improve quality of life.