Cannabinoid CB_(2) receptors and spinal microglia are implicated in tingenone-mediated antinociception in mice

Objective:To investigate the antinociceptive effect of tingenone on inflammatory pain,as well as and the involvement of the cannabinoid receptors type 2(CB2)and spinal microglia in this process.Methods:Male Swiss mice were subjected to inflammatory pain induced by intraplantar injection of carrageenan.The nociceptive threshold was measured by von Frey filaments test.Tingenone was administered orally 60 min before carrageenan injection.To evaluate the involvement of CB2 receptor,endocannabinoids,and microglia,AM630(a CB2 receptor antagonist),MAFP(an inhibitor of an enzyme that hydrolyses endocannabinoids),and minocycline(a microglial inhibitor)were given intrathecally 20 min before tingenone administration.In addition,an immunofluorescence assay was used to evaluate CB2 receptor and CD11 B(a microglial marker)expression in the spinal cord dorsal horn.Results:Tingenone significantly reduced carrageenan-induced hyperalgesia,which was reversed by pretreatment with AM630.MAFP and minocycline potentiated and prolonged the tingenoneinduced antinociception.CD11 B expression was increased in the spinal cord dorsal horn of mice with inflammatory pain pretreated with tingenone,which was reduced by AM630,MAFP,and minocycline.Conclusions:CB2 receptors and endocannabinoids participate in the tingenone-induced antinociception which may involve the inhibition of microglia at spinal level.

Effect of a cardiac telerehabilitation program during COVID-19 associated social isolation

This study aimed to compare the impact of a cardiac telerehabilitation(CTR)protocol aimed at patients with cardiovascular diseases(CVDs)during the period of coronavirus disease 2019(COVID-19)associated with social isolation.This retrospective cohort study included 58 participants diagnosed with stable cardiovascular diseases(CVDs),which were divided into three groups:conventional cardiac rehabilitation(CCR)group(n=20),composed of patients undergoing conventional cardiac rehabilitation;cardiac telerehabilitation(CTR)group(n=18),composed of patients undergoing cardiac telerehabilitation and control group(n=20),composed of patients admitted for cardiac rehabilitation who had not started training programs.The results showed that body mass index was reduced(p=0.019)and quality of life was improved(e.g.,limitations due to physical aspects[p=0.021),vitality[p=0.045]and limitations due to emotional aspects[p=0.024])by CCR compared to baseline.These outcomes were not improved by CTR(p>0.05).However,this strategy prevented clinical deterioration in the investigated patients.Although CCR achieved a superior effect on clinical improvement and quality of life,CTR was relevant to stabilize the blood pressure and quality of life of patients with cardiovascular diseases during the period of COVID-19-associated social isolation.