Gibberellins(GAs)are a class of plant hormones that can affect plant growth and development.GA-oxidases are rate-limiting enzymes,which play a direct role in GA accumulation in plants.However,the roles of GA-oxidase o...Gibberellins(GAs)are a class of plant hormones that can affect plant growth and development.GA-oxidases are rate-limiting enzymes,which play a direct role in GA accumulation in plants.However,the roles of GA-oxidase on carrot(Daucus carota L.)taproot development are still unclear.In this study,two GA-oxidase genes,DcGA20ox2 and DcGA2ox1,were identified in carrot.Transgenic carrot plants were obtained by using Agrobacterium-mediated genetic transformation method.The results showed that overexpression of DcGA20ox2 significantly promoted the accumulation of active GAs in carrot,increased plant height,generated more branches,and enhanced xylem development.Overexpression of DcGA2ox1 significantly reduced the total contents of active GAs compared with the control group,resulting in a dwarf phenotype and markedly increased lignin content of the transgenic carrot.The expression profiling showed that the genes of GA metabolic pathway responded to the negative feedback regulation mechanism.At the same time,the expression of most genes in lignin biosynthesis and polymerization process was up-regulated,corresponding to the massive accumulation of lignin.These findings indicated that DcGA20ox2 and DcGA2ox1affected carrot growth and development by regulating the levels of endogenous GAs.The results from current work might shed light on further studies aimed to regulate lignification in carrot and other crops.展开更多
In recent years,using network pharmacology and molecular docking technology to reveal the therapeutic targets of irritable bowel syndrome(IBS)has become a research hotspot.This paper summarizes 31 studies of tradition...In recent years,using network pharmacology and molecular docking technology to reveal the therapeutic targets of irritable bowel syndrome(IBS)has become a research hotspot.This paper summarizes 31 studies of traditional Chinese medicine for IBS by domestic and foreign scholars,and expounds the action mechanism of single drug,drug pair and compound prescription in the treatment of IBS from the aspects of drug activity,action target,biological process and pathway analysis.It also discusses the shortcomings and prospects of network pharmacology and molecular docking technology in the field of traditional Chinese medicine research,and provides reference for expanding the research ideas of traditional Chinese medicine in treating IBS.展开更多
Huaiyin Institute of Technology is taken as the research object.The content of horticulture curriculum system under the background of new agricultural science is constructed and elaborated,including theoretical curric...Huaiyin Institute of Technology is taken as the research object.The content of horticulture curriculum system under the background of new agricultural science is constructed and elaborated,including theoretical curriculum system of"three platforms and seven curriculum groups"and practice system of"two platforms,three levels and four modules".Moreover,the quality assurance strategy of the curriculum system is discussed from three angles of enhancing professional emotion,enhancing professional practice and strengthening innovation ability,to reach the training objective of outstanding applied horticultural talents under the background of new agricultural science.展开更多
Using the structural vector autoregression model, we estimate the current responses of monetary policy to contemporaneous shocks from macroeconomic variables. Our findings indicate that the People＇s Bank of China res...Using the structural vector autoregression model, we estimate the current responses of monetary policy to contemporaneous shocks from macroeconomic variables. Our findings indicate that the People＇s Bank of China responded to inflation and output changes, but did not react to asset price fluctuations during the period from January 1997 to March 2010. The optimal monetary responses to exogenous shocks are also examined It is revealed that using asset prices to formulate monetary policy would not help to improve monetary authorities ＇performance in lowering the volatilities of output growth and inflation while keeping output growth and inflation in their safety zones. The effectiveness of monetary policy and fiscal policy in reacting to external shocks is also discussed.展开更多
基金supported by the National Natural Science Foundation of China(Grant Nos.3210236932372681)+1 种基金Natural Science Foundation of Jiangsu Province(BK20211366)the Priority Academic Program Development of Jiangsu Higher Education Institutions Project(PAPD)。
文摘Gibberellins(GAs)are a class of plant hormones that can affect plant growth and development.GA-oxidases are rate-limiting enzymes,which play a direct role in GA accumulation in plants.However,the roles of GA-oxidase on carrot(Daucus carota L.)taproot development are still unclear.In this study,two GA-oxidase genes,DcGA20ox2 and DcGA2ox1,were identified in carrot.Transgenic carrot plants were obtained by using Agrobacterium-mediated genetic transformation method.The results showed that overexpression of DcGA20ox2 significantly promoted the accumulation of active GAs in carrot,increased plant height,generated more branches,and enhanced xylem development.Overexpression of DcGA2ox1 significantly reduced the total contents of active GAs compared with the control group,resulting in a dwarf phenotype and markedly increased lignin content of the transgenic carrot.The expression profiling showed that the genes of GA metabolic pathway responded to the negative feedback regulation mechanism.At the same time,the expression of most genes in lignin biosynthesis and polymerization process was up-regulated,corresponding to the massive accumulation of lignin.These findings indicated that DcGA20ox2 and DcGA2ox1affected carrot growth and development by regulating the levels of endogenous GAs.The results from current work might shed light on further studies aimed to regulate lignification in carrot and other crops.
文摘In recent years,using network pharmacology and molecular docking technology to reveal the therapeutic targets of irritable bowel syndrome(IBS)has become a research hotspot.This paper summarizes 31 studies of traditional Chinese medicine for IBS by domestic and foreign scholars,and expounds the action mechanism of single drug,drug pair and compound prescription in the treatment of IBS from the aspects of drug activity,action target,biological process and pathway analysis.It also discusses the shortcomings and prospects of network pharmacology and molecular docking technology in the field of traditional Chinese medicine research,and provides reference for expanding the research ideas of traditional Chinese medicine in treating IBS.
基金Supported by Higher Education Research Project of Huaiyin Institute of Technology(2019GJ12).
文摘Huaiyin Institute of Technology is taken as the research object.The content of horticulture curriculum system under the background of new agricultural science is constructed and elaborated,including theoretical curriculum system of"three platforms and seven curriculum groups"and practice system of"two platforms,three levels and four modules".Moreover,the quality assurance strategy of the curriculum system is discussed from three angles of enhancing professional emotion,enhancing professional practice and strengthening innovation ability,to reach the training objective of outstanding applied horticultural talents under the background of new agricultural science.
基金the National Natural Science Foundation of China(Grant No.70841023)
文摘Using the structural vector autoregression model, we estimate the current responses of monetary policy to contemporaneous shocks from macroeconomic variables. Our findings indicate that the People＇s Bank of China responded to inflation and output changes, but did not react to asset price fluctuations during the period from January 1997 to March 2010. The optimal monetary responses to exogenous shocks are also examined It is revealed that using asset prices to formulate monetary policy would not help to improve monetary authorities ＇performance in lowering the volatilities of output growth and inflation while keeping output growth and inflation in their safety zones. The effectiveness of monetary policy and fiscal policy in reacting to external shocks is also discussed.
