Platelet RNA enables accurate detection of ovarian cancer:an intercontinental,biomarker identification study

Platelets are reprogrammed by cancer via a process called education,which favors cancer development.The transcriptional profile of tumor-educated platelets(TEPs)is skewed and therefore practicable for cancer detection.This intercontinental,hospital-based,diagnostic study included 761 treatment-naive inpatients with histologically confirmed adnexal masses and 167 healthy controls from nine medical centers(China,n=3;Netherlands,n=5;Poland,n=1)between September 2016 and May 2019.The main outcomes were the performance of TEPs and their combination with CA125 in two Chinese(VC1 and VC2)and the European(VC3)validation cohorts collectively and independently.Exploratory outcome was the value of TEPs in public pan-cancer platelet transcriptome datasets.The AUCs for TEPs in the combined validation cohort,VC1,VC2,and VC3 were 0.918(95%CI 0.889-0.948),0.923(0.855-0.990),0.918(0.872-0.963),and 0.887(0.813-0.960),respectively.Combination of TEPs and CA125 demonstrated an AUC of 0.922(0.889-0.955)in the combined validation cohort;0.955(0.912-0.997)in VC1;0.939(0.901-0.977)in VC2;0.917(0.824-1.000)in VC3.For subgroup analysis,TEPs exhibited an AUC of o.858,0.859,and 0.920 to detect early-stage,borderline,non-epithelial diseases and 0.899 to discriminate ovarian cancer from endometriosis.TEPs had robustness,compatibility,and universality for preop.erative diagnosis of ovarian cancer since it withstood validations in populations of different ethnicities,heterogeneous histoiogical subtypes,and early-stage ovarian cancer.However,these observations warrant prospective validations in a larger population beforeclinicalutilities.

Spectrum of Diseases in Female Children and Adolescents Admitted to the Obstetrics and Gynecology Hospital, Shanghai, between 2003 and 2017

Objective:To investigate the spectrum of diseases in female children and adolescents hospitalized for obstetric and gynecological conditions.Methods:This retrospective study included patients(age≤19 years)admitted to the Obstetrics and Gynecology Hospital of Fudan University,Shanghai,between January 2003 and December 2017.Their medical records-age,principal diagnosis,and treatment-were reviewed and analyzed.Diagnoses were divided into eight disease classifications.Comparison between groups was analyzed with Student’s t-test.Results:This study enrolled 4,628 inpatients.The number of inpatients increased each year,especially in the groups of gynecologic tumors,pregnancy-related conditions,and disorders of the breast.The mean age was 16.33±2.42 years(range:2-19 years).Of all the inpatients,41.9%(n=1,939)were hospitalized for gynecologic tumors,and 85.5%(n=1,657)of the tumors were benign.Altogether,ovarian cysts(28.66%,n=1,325)were the most common disease observed in this group.In addition,32.9%(n=1,524)of the inpatients were hospitalized for pregnancy,childbirth,and puerperium;within this group,21.9%(n=1,014)were hospitalized for artificial abortions.Conclusions:The efforts of all stakeholders toward the implementation of government policies to promote sexual and reproductive health among children and adolescents in China have been effective.

Platelet RNA signature independently predicts ovarian cancer prognosis by deep learning neural network model

Dear Editor,Platelets are circulating anucleate cytoplasmic fragments of megakaryocytes and characterized by their functions in wound healing and vascular integrity maintenance.Increasing evidence highlights the extensive reciprocal signaling interactions between platelets and tumor cells(Haemmerle et al.,2018).Tumor cells activate and aggregate platelets to sustain proliferation(Cho et al.,2012),resist apoptosis,and promote metastasis(Haemmerle et al.,2017).