Microorganism DNA of rhizosphere soil from Pinus koraiensis and Pinus sylvestriformis were extracted by proteinase K based on SDS method, CTAB method, PVP （polyvinylpolypyrrolidone） method, and freezing and thawing ...Microorganism DNA of rhizosphere soil from Pinus koraiensis and Pinus sylvestriformis were extracted by proteinase K based on SDS method, CTAB method, PVP （polyvinylpolypyrrolidone） method, and freezing and thawing method and the crude DNA from rhizosphere soil were purified by dialysis method, silver beads absorption method, and squeezing DNA gel method. The results of different extracting and purifying methods were compared and evaluated. Results indicated that the best method of extraction for microorganism DNA in rhizosphere soil was proteinse K based on SDS method with high salt concentration of 1.0% （w/v） NaCl, which could effectively eliminate humic acids and other impurities. The dialysis method was suitable to purify DNA from rhizosphere soil because of effectively removing brown matters and humic acids and the purified products were suited to PCR amplification. Squeezing DNA gel method was also a good purification method with the advantage of inexpensive in cost and efficient in use.展开更多
Genetic diversities and genetic differentiations of the four Pinus koraiensis populations （Gaofeng Forestry Farm at Tangwanghe in Yichun City, Erdaobaihe Town in the Changbai Mountains, Shcngshan Forestry Farm in Hei...Genetic diversities and genetic differentiations of the four Pinus koraiensis populations （Gaofeng Forestry Farm at Tangwanghe in Yichun City, Erdaobaihe Town in the Changbai Mountains, Shcngshan Forestry Farm in Heihe City, in China, and the suburb of Vladivostok City in Russia） were analyzed by using ISSR PCR technique. The results of 15 primers amplification showed that the ratio of polymorphic site of P koralensis population was 60.7%. Euch primer had 3.6 polymorphic sites, The diversity levels of the four P. koraiensis populations were rather higher compared with those of other Pinaceae species. The genetic diversity levels of t： koraiensis populations grown in the center region were higher than those grown in the edge zone. The genetic diversity of r3 koraiensis mainly came from the interiot of the population, accounting for 73% of total genetic diversity. There were no positive correlation between genetic distances and geographical distances for the four P koraiensis populations. The gradual decrease of natural distribution region of P. koraiensis was due to anthropic destroy and environmental factors （i.e. fire and wind throw）, rather than the lower genetic diversity.展开更多
Coarse woody debris is an important structure and function unit in forest ecosystem.This review analyzed the ecological functions of coarse woody debris in forest ecosystem and introduced several hotspots and existing...Coarse woody debris is an important structure and function unit in forest ecosystem.This review analyzed the ecological functions of coarse woody debris in forest ecosystem and introduced several hotspots and existing problems in coarse woody debris research field. It is suggested that quantitative research should be intensified in theintensified in the ecological demands of coarse woody debris for providing a technical guidelines in management of productivity, biodiversity and other ecologicalprocesses.展开更多
Soil samples were taken from an Ermans birch （Betula ermanii）-dark coniferous forest （Picea jezoensis and Abies nephrolepis） ecotone growing on volcanic ejecta in the northern slope of Changbai Mountains of Northe...Soil samples were taken from an Ermans birch （Betula ermanii）-dark coniferous forest （Picea jezoensis and Abies nephrolepis） ecotone growing on volcanic ejecta in the northern slope of Changbai Mountains of Northeast China, to compare soil carbon （C） and nitrogen （N） transformations in the two forests. The soil type is Umbri-Gelic Cambosols in Chinese Soil Taxonomy. Soil samples were incubated aerobically at 20℃ and field capacity of 700 g kg^-1 over a period of 27 weeks. The amount of soil microbial biomass and net N mineralization were higher in the Ermans birch than the dark coniferous forest （P 〈 0.05）, whereas the cumulative C mineralization （as CO2 emission） in the dark coniferous forest exceeded that in the Ermans birch （P 〈 0.05）. Release of the cumulative dissolved organic C and dissolved organic N were greater in the Ermans birch than the dark coniferous forest （P 〈 0.05）. The results suggested that differences of forest types could result in considerable change in soil C and N transformations.展开更多
The objectives of this study were to investigate the effect of higher CO2 concentrations （500 and 700 μmol mol^-1） in atmosphere on total soil respiration and the contribution of root respiration to total soil resp...The objectives of this study were to investigate the effect of higher CO2 concentrations （500 and 700 μmol mol^-1） in atmosphere on total soil respiration and the contribution of root respiration to total soil respiration during seedling growth of Pinus sylvestris vat. sylvestriformis. During the four growing seasons （May-October） from 1999 to 2003, the seedlings were exposed to elevated concentrations of CO2 in open-top chambers. The total soil respiration and contribution of root respiration were measured using an LI-6400-09 soil CO2 flux chamber on June 15 and October 8, 2003. To separate root respiration from total soil respiration, three PVC cylinders were inserted approximately 30 cm deep into the soil in each chamber. There were marked diurnal changes in air and soil temperatures on June 15. Both the total soil respiration and the soil respiration without roots showed a strong diurnal pattern, increasing from before sunrise to about 14：00 in the afternoon and then decreasing before the next sunrise. No increase in the mean total soil respiration and mean soil respiration with roots severed was observed under the elevated CO2 treatments on June 15, 2003, as compared to the open field and control chamber with ambient CO2. However, on October 8, 2003, the total soil respiration and soil respiration with roots severed in the open field were lower than those in the control and elevated CO2 chambers. The mean contribution of root respiration measured on June 15, 2003, ranged from 8.3% to 30.5% and on October 8, 2003, from 20.6% to 48.6%.展开更多
Twenty-three secondary forest communities with different structure were selected in Mao＇er Mountain National Park of Heilongjiang Province, China to study the relationship between diversity of forest plant species an...Twenty-three secondary forest communities with different structure were selected in Mao＇er Mountain National Park of Heilongjiang Province, China to study the relationship between diversity of forest plant species and environmental gradient. The forest plant species diversity was analyzed by the diversity index, and the environmental factors was quantified by the method of Whittaker＇s quantification of environmental gradient. Meanwhile, β-diversity indexes of communities were calculated with similar measurements. The results showed that the Shannon-wiener diversity index of forest plant species increased with the increase of the environmental gradient, and the β-diversity indexes of communities showed a liner increase along with the change of environmental gradient.展开更多
Responses of soil microbial activities to elevated CO, in experiment sites of Pinus sylvestriformis and Pinus koratensts seecllmgs were studied in summer in 2003. The results indicated the number of bacteria decreased...Responses of soil microbial activities to elevated CO, in experiment sites of Pinus sylvestriformis and Pinus koratensts seecllmgs were studied in summer in 2003. The results indicated the number of bacteria decreased significantly （p 〈 0.05） under elevated CO, for Pinus syivestriformis and Pinups koraiensis. Amylase and invertase activities in soil increased for Pinus syivestriformis and decreased for Pinus koraiensis with CO2 enrichment compared with those at ambient （350 pmol·mol^-1）. The size of microbial biomass C also decreased significantly at 700 μmol- mol^-1 CO2. Bacterial community structure had some evident changes under elevated CO, by DGGE （Denaturing Gradient Gel Electrophoresis） analysis of bacterial 16S rDNA gene fragments amplified by PCR from DNA extracted directly from soil. The results suggested that responses of soil microorganisms to elevated CO2 would be related to plant species exposed to elevated CO2.展开更多
The biodiversity was studied in 26 communities with different structures in Maoershan National Park and Liangshui Natural Reserve of Northeast Forestry University in Heilongjiang Province, China. Composition index （C...The biodiversity was studied in 26 communities with different structures in Maoershan National Park and Liangshui Natural Reserve of Northeast Forestry University in Heilongjiang Province, China. Composition index （CI） was taken as a parameter to quantify the community dynamics, which can nicely describe forest community dynamics, meanwhile, the relationship between diversity and community dynamics were also investigated and analyzed. Results showed that the total number species of community, richness, evenness, and Shannon-Wiener diversity index were obviously different in every community. The richness decreased with the increasing CI of every community, which means richness was in inverse proportion to community dynamics. The Shannon-Wiener index of every community increased from the initial stage to the middle stage of succession, and then decreased in the climax stage. The coverage weighted foliage-height diversity index increased along with the increase of CI, which was similar as the oattem diversity.展开更多
Understanding the responses of soil organic carbon(SOC) fractions to altitudinal gradient variation is important for understanding changes in the carbon balance of forest ecosystems.In our study the SOC and its fracti...Understanding the responses of soil organic carbon(SOC) fractions to altitudinal gradient variation is important for understanding changes in the carbon balance of forest ecosystems.In our study the SOC and its fractions of readily oxidizable carbon(ROC),water-soluble carbon(WSC) and microbial biomass carbon(MBC) in the soil organic and mineral horizons were investigated for four typical forest types,including mixed coniferous broad-leaved forest(MCB),dark coniferous spruce-fir forest(DCSF),dark coniferous spruce forest(DCS),and Ermans birch forest(EB),along an altitudinal gradient in the Changbai Mountain Nature Reserve in Northeast China.The results showed that there was no obvious altitudinal pattern in the SOC.Similar variation trends of SOC with altitude were observed between the organic and mineral horizons.Significant differences in the contents of SOC,WSC,MBC and ROC were found among the four forest types and between horizons.The contents of ROC in the mineral horizon,WSC in the organic horizon and MBC in both horizons in the MCB and EB forests were significantly greater than those in either DCSF or DCS forest.The proportion of soil WSC to SOC was the lowest among the three main fractions.The contents of WSC,MBC and ROC were significantly correlated(P < 0.05) with SOC content.It can be concluded that vegetation types and climate were crucial factors in regulating the distribution of soil organic carbon fractions in Changbai Mountain.展开更多
基金This project was supported by the Knowledge Innovation Project from Chinese Academy （KZCXI-SW-01） and the National Natural Science Foundation of China （30070158）.
文摘Microorganism DNA of rhizosphere soil from Pinus koraiensis and Pinus sylvestriformis were extracted by proteinase K based on SDS method, CTAB method, PVP （polyvinylpolypyrrolidone） method, and freezing and thawing method and the crude DNA from rhizosphere soil were purified by dialysis method, silver beads absorption method, and squeezing DNA gel method. The results of different extracting and purifying methods were compared and evaluated. Results indicated that the best method of extraction for microorganism DNA in rhizosphere soil was proteinse K based on SDS method with high salt concentration of 1.0% （w/v） NaCl, which could effectively eliminate humic acids and other impurities. The dialysis method was suitable to purify DNA from rhizosphere soil because of effectively removing brown matters and humic acids and the purified products were suited to PCR amplification. Squeezing DNA gel method was also a good purification method with the advantage of inexpensive in cost and efficient in use.
文摘Genetic diversities and genetic differentiations of the four Pinus koraiensis populations （Gaofeng Forestry Farm at Tangwanghe in Yichun City, Erdaobaihe Town in the Changbai Mountains, Shcngshan Forestry Farm in Heihe City, in China, and the suburb of Vladivostok City in Russia） were analyzed by using ISSR PCR technique. The results of 15 primers amplification showed that the ratio of polymorphic site of P koralensis population was 60.7%. Euch primer had 3.6 polymorphic sites, The diversity levels of the four P. koraiensis populations were rather higher compared with those of other Pinaceae species. The genetic diversity levels of t： koraiensis populations grown in the center region were higher than those grown in the edge zone. The genetic diversity of r3 koraiensis mainly came from the interiot of the population, accounting for 73% of total genetic diversity. There were no positive correlation between genetic distances and geographical distances for the four P koraiensis populations. The gradual decrease of natural distribution region of P. koraiensis was due to anthropic destroy and environmental factors （i.e. fire and wind throw）, rather than the lower genetic diversity.
基金This work was supported by National Natural Science Foundation of China（No.30370293）,0pen Fund for the Research Station of Changbai Mountain Forest Ecosystem.Chinese Academy of Sciences, and Science Frontiers Project of Institute of Applied Ecology, Chinese Academy of Sciences.
文摘Coarse woody debris is an important structure and function unit in forest ecosystem.This review analyzed the ecological functions of coarse woody debris in forest ecosystem and introduced several hotspots and existing problems in coarse woody debris research field. It is suggested that quantitative research should be intensified in theintensified in the ecological demands of coarse woody debris for providing a technical guidelines in management of productivity, biodiversity and other ecologicalprocesses.
基金Project supported by the National Natural Science Foundation of China(No.90411020)the National Key Basic Research Program(973 Program)of China(No.2002CB412502)
文摘Soil samples were taken from an Ermans birch （Betula ermanii）-dark coniferous forest （Picea jezoensis and Abies nephrolepis） ecotone growing on volcanic ejecta in the northern slope of Changbai Mountains of Northeast China, to compare soil carbon （C） and nitrogen （N） transformations in the two forests. The soil type is Umbri-Gelic Cambosols in Chinese Soil Taxonomy. Soil samples were incubated aerobically at 20℃ and field capacity of 700 g kg^-1 over a period of 27 weeks. The amount of soil microbial biomass and net N mineralization were higher in the Ermans birch than the dark coniferous forest （P 〈 0.05）, whereas the cumulative C mineralization （as CO2 emission） in the dark coniferous forest exceeded that in the Ermans birch （P 〈 0.05）. Release of the cumulative dissolved organic C and dissolved organic N were greater in the Ermans birch than the dark coniferous forest （P 〈 0.05）. The results suggested that differences of forest types could result in considerable change in soil C and N transformations.
基金Project supported by the Knowledge Innovation Project of the Chinese Academy of Sciences (No.KZCX2-YW-416)National NaturM Science Foundation of China (No.90411020)
文摘The objectives of this study were to investigate the effect of higher CO2 concentrations （500 and 700 μmol mol^-1） in atmosphere on total soil respiration and the contribution of root respiration to total soil respiration during seedling growth of Pinus sylvestris vat. sylvestriformis. During the four growing seasons （May-October） from 1999 to 2003, the seedlings were exposed to elevated concentrations of CO2 in open-top chambers. The total soil respiration and contribution of root respiration were measured using an LI-6400-09 soil CO2 flux chamber on June 15 and October 8, 2003. To separate root respiration from total soil respiration, three PVC cylinders were inserted approximately 30 cm deep into the soil in each chamber. There were marked diurnal changes in air and soil temperatures on June 15. Both the total soil respiration and the soil respiration without roots showed a strong diurnal pattern, increasing from before sunrise to about 14：00 in the afternoon and then decreasing before the next sunrise. No increase in the mean total soil respiration and mean soil respiration with roots severed was observed under the elevated CO2 treatments on June 15, 2003, as compared to the open field and control chamber with ambient CO2. However, on October 8, 2003, the total soil respiration and soil respiration with roots severed in the open field were lower than those in the control and elevated CO2 chambers. The mean contribution of root respiration measured on June 15, 2003, ranged from 8.3% to 30.5% and on October 8, 2003, from 20.6% to 48.6%.
基金Foundation project： The paper was supported by National Natural Science Foundation of China （39899370）.
文摘Twenty-three secondary forest communities with different structure were selected in Mao＇er Mountain National Park of Heilongjiang Province, China to study the relationship between diversity of forest plant species and environmental gradient. The forest plant species diversity was analyzed by the diversity index, and the environmental factors was quantified by the method of Whittaker＇s quantification of environmental gradient. Meanwhile, β-diversity indexes of communities were calculated with similar measurements. The results showed that the Shannon-wiener diversity index of forest plant species increased with the increase of the environmental gradient, and the β-diversity indexes of communities showed a liner increase along with the change of environmental gradient.
基金The study was supported by Major State Basic Research Development Program of China (2002CB412502) and the Knowledge Inno-vation Project from Chinese Academy of Sciences (KZCX1-SW-01-03).
文摘Responses of soil microbial activities to elevated CO, in experiment sites of Pinus sylvestriformis and Pinus koratensts seecllmgs were studied in summer in 2003. The results indicated the number of bacteria decreased significantly （p 〈 0.05） under elevated CO, for Pinus syivestriformis and Pinups koraiensis. Amylase and invertase activities in soil increased for Pinus syivestriformis and decreased for Pinus koraiensis with CO2 enrichment compared with those at ambient （350 pmol·mol^-1）. The size of microbial biomass C also decreased significantly at 700 μmol- mol^-1 CO2. Bacterial community structure had some evident changes under elevated CO, by DGGE （Denaturing Gradient Gel Electrophoresis） analysis of bacterial 16S rDNA gene fragments amplified by PCR from DNA extracted directly from soil. The results suggested that responses of soil microorganisms to elevated CO2 would be related to plant species exposed to elevated CO2.
基金The paper was supported by National Natural Science Foundation of China (39899370).
文摘The biodiversity was studied in 26 communities with different structures in Maoershan National Park and Liangshui Natural Reserve of Northeast Forestry University in Heilongjiang Province, China. Composition index （CI） was taken as a parameter to quantify the community dynamics, which can nicely describe forest community dynamics, meanwhile, the relationship between diversity and community dynamics were also investigated and analyzed. Results showed that the total number species of community, richness, evenness, and Shannon-Wiener diversity index were obviously different in every community. The richness decreased with the increasing CI of every community, which means richness was in inverse proportion to community dynamics. The Shannon-Wiener index of every community increased from the initial stage to the middle stage of succession, and then decreased in the climax stage. The coverage weighted foliage-height diversity index increased along with the increase of CI, which was similar as the oattem diversity.
基金Supported by the National Basic Research Program (973 Program) of China (No.2011CB403204)the Youth Fund for Creative Research Groups,Institute of Applied Ecology,Chinese Academy of Sciences
文摘Understanding the responses of soil organic carbon(SOC) fractions to altitudinal gradient variation is important for understanding changes in the carbon balance of forest ecosystems.In our study the SOC and its fractions of readily oxidizable carbon(ROC),water-soluble carbon(WSC) and microbial biomass carbon(MBC) in the soil organic and mineral horizons were investigated for four typical forest types,including mixed coniferous broad-leaved forest(MCB),dark coniferous spruce-fir forest(DCSF),dark coniferous spruce forest(DCS),and Ermans birch forest(EB),along an altitudinal gradient in the Changbai Mountain Nature Reserve in Northeast China.The results showed that there was no obvious altitudinal pattern in the SOC.Similar variation trends of SOC with altitude were observed between the organic and mineral horizons.Significant differences in the contents of SOC,WSC,MBC and ROC were found among the four forest types and between horizons.The contents of ROC in the mineral horizon,WSC in the organic horizon and MBC in both horizons in the MCB and EB forests were significantly greater than those in either DCSF or DCS forest.The proportion of soil WSC to SOC was the lowest among the three main fractions.The contents of WSC,MBC and ROC were significantly correlated(P < 0.05) with SOC content.It can be concluded that vegetation types and climate were crucial factors in regulating the distribution of soil organic carbon fractions in Changbai Mountain.
