On the ＂real people＂ in social security -Based on the existing social security system in China

In Chinese the existing social security system mode, all the provinces of the endowment insurance, social welfare, medical care and other aspects there are many problems, such as the loss of independence for the elderly pension problems, nursing homes, the contradiction between supply and demand of the elderly spiritual comfort and social medical assistance etc. there was a lot of trouble, but also formulated by the state the social security system does not take into account the above situation, this article mainly from the angle analysis, we propose to combine the actual situation in the present and make some laws and regulations, through the redistribution of national finance, provide material guarantee to solve the fundamental contradiction of reality and to solve this problem fundamentally, to avoid the occurrence of social conflicts. The discussion of the people in the reality of social security has the double meaning of theory and practice.

组蛋白乙酰转移酶MYST2对胰腺癌的影响

目的探讨组蛋白乙酰转移酶MYST2在胰腺癌细胞的蛋白质表达水平及其对诊断胰腺癌的价值。方法收集2017年12月1日至2020年6月30日于北京协和医院行手术切除并经病理学确诊的54例胰腺癌患者的癌组织和对应癌旁组织(距离手术切缘>5 cm)。对人胰腺癌细胞系ASPC1和BXPC3进行敲低处理(ASPC1敲低组和BXPC3敲低组),对CFPAC1和SW1990进行过表达处理(CFPAC1过表达组和SW1990过表达组),未经处理的为ASPC1、BXPC3、CFPAC1和SW1990空载对照组。采用蛋白质印迹法分别检测不同病理分化程度患者的胰腺癌细胞,人正常胰腺导管上皮细胞系HPDE和人胰腺癌细胞系ASPC1、BXPC3、CFPAC1、SW1990,以及敲低组、过表达组和空载对照组的MYST2蛋白质水平。采用实时无标记动态细胞分析技术,Transwell迁移、侵袭实验和平板集落形成实验比较敲低组、过表达组与空载对照组细胞的增殖活性,迁移、侵袭和集落形成能力。对54例患者的胰腺癌及其癌旁组织进行MYST2免疫组织化学评分,并绘制受试者操作特征曲线分析MYST2不同表达水平对诊断胰腺癌的价值。统计学方法采用独立样本t检验和方差分析。结果54例胰腺癌患者病理切片中,高分化13例,中分化24例,低分化15例,未分化2例,胰腺癌细胞MYST2蛋白质表达水平分别为3.12±1.67、2.87±1.59、2.12±1.03、1.08±0.34,差异有统计学意义(F=1.241,P<0.05)。4种胰腺癌细胞系ASPC1、BXPC3、CFPAC1、SW1990的MYST2表达水平均高于正常胰腺导管上皮细胞系HPDE(1.41±0.47、1.40±0.93、1.13±0.62、1.71±0.46比0.82±0.25),差异均有统计学意义(t=1.625、1.577、1.319、1.832,P均<0.05)。BXPC3敲低组MYST2水平低于BXPC3空载对照组(0.39±0.12比0.75±0.34),ASPC1敲低组低于ASPC1空载对照组(0.43±0.22比0.82±0.48),CFPAC1过表达组高于CFPAC1空载对照组(1.38±0.45比0.82±0.37),SW1990过表达组高于SW1990空载对照组(1.34±0.65比0.51±0.22),差异均有统计学意义(t=1.414、1.378、1.319、1.934,P均<0.05)。ASPC1敲低组细胞增殖较ASPC1空载对照组缓慢,80 h增殖峰值低于空载对照组(1.02±0.77比4.31±2.45);BXPC3敲低组细胞增殖较BXPC3空载对照组缓慢,80 h增殖峰值低于空载对照组(0.91±0.24比2.84±0.53);SW1990过表达组胰腺癌细胞增殖较SW1990空载对照组快,80 h增殖峰值高于空载对照组(3.10±0.67比1.04±0.17);CFPAC1过表达组胰腺癌细胞增殖较CFPAC1空载对照组快,80 h增殖峰值高于空载对照组(5.45±1.13比1.01±0.29),差异均有统计学意义(t=1.427、1.316、1.292、1.501,P均<0.05)。在迁移能力检测中,ASPC1敲低组穿过Transwell小室的细胞少于ASPC1空载对照组(34.08±17.62比118.76±5.31),BXPC3敲低组少于BXPC3空载对照组(18.62±9.64比57.90±12.67);SW1990过表达组多于SW1990空载对照组(134.84±24.65比37.82±6.73),CFPAC1过表达组多于CFPAC1空载对照组(65.79±27.46比11.68±5.13),差异均有统计学意义(t=1.475、1.322、1.437、1.219,P均<0.05)。在侵袭能力检测中,ASPC1敲低组穿过Transwell小室的细胞少于ASPC1空载对照组(9.79±5.75比45.76±12.71),BXPC3敲低组少于BXPC3空载对照组(23.46±11.13比84.92±17.65),SW1990过表达组多于SW1990空载对照组(156.42±34.50比42.13±22.17),CFPAC1过表达组多于CFPAC1空载对照组(112.64±47.82比39.09±17.23),差异均有统计学意义(t=1.324、1.635、1.423、1.119,P均<0.05)。在集落形成数量方面,ASPC1敲低组少于ASPC1空载对照组(13.15±6.42比86.79±35.17),BXPC3敲低组少于BXPC3空载对照组(14.93±9.30比52.93±15.76);SW1990过表达组多于SW1990空载对照组(129.10±57.31比62.42±37.43),CFPAC1过表达组多于CFPAC1空载对照组(157.98±66.45比74.35±34.69),差异均有统计学意义(t=1.148、1.290、1.274、1.462,P均<0.05)。胰腺癌组织MYST2评分高于癌旁胰腺组织[(3.04±2.23)分比(1.32±0.70)分],差异有统计学意义(t=3.479,P<0.05)。当MYST免疫组织化学总评分为3分时,曲线下面积最大(0.888,95%可信区间0.827~0.948),约登指数为0.56。结论MYST2与胰腺癌细胞增殖、侵袭、迁移能力有关,其高表达可促进胰腺癌发展。