This essay endeavors to dispel the lingering misconception that Li Bai,the celebrated Tang Dynasty poet,was a man devoid of propriety.Through a meticulous examination of historical records,literary works,and contempor...This essay endeavors to dispel the lingering misconception that Li Bai,the celebrated Tang Dynasty poet,was a man devoid of propriety.Through a meticulous examination of historical records,literary works,and contemporary interpretations,this study presents a nuanced portrait of Li Bai as a poet who,despite his eccentricities and wanderlust,possessed a profound sense of etiquette and respect for societal norms.This essay argues that his unique personality in life and free expression in poetry are all driven by the need for artistic creation,rather than challenging for ancient propriety.展开更多
Background Oncofetal protein high-mobility-group AT-hook protein 2 （HMGA2） is reactivated in serous ovarian cancer （SOC） and its overexpression correlates with poor prognosis.To explore the mechanism,we investigat...Background Oncofetal protein high-mobility-group AT-hook protein 2 （HMGA2） is reactivated in serous ovarian cancer （SOC） and its overexpression correlates with poor prognosis.To explore the mechanism,we investigated whether HMGA2 could avoid microRNA regulation due to gene truncation or 3＇ UTR shortening by alternative polyadenylation.Methods Real-time reverse transcription polymerase chain reaction （RT-PCR） was used to evaluate the abundance of different regions of HMGA2 mRNA in 46 SOC samples.Rapid amplification of cDNA 3＇ ends （3＇ RACE） and Southern blotting were used to confirm the shortening of 3＇ untranslated region （UTR）.5＇ RACE and Southern blotting were used to prove the mRNA decay.Results No significant difference in the ratio of the stable coding region to the fragile region was observed between SOC and control normal fallopian tubes,indicating that the HMGA2 gene is not truncated in SOC.Varying degrees of 3＇ UTR shortening in SOC samples were observed by comparing the abundance of the proximal region and distal region of the HMGA2 3＇ UTR.The ratio of the proximal to the distal region of the 3＇ UTR correlated significantly with expression of the HMGA2 coding region in SOC （r=0.579,P ＜0.01）.Moreover,although the abundance of the HMGA2 coding region varied,all samples,including the very low expressed samples,exhibit relatively high levels of the proximal 3＇ UTR region,suggesting a dynamic decay of HMGA2 mRNA from the 5＇ end.The shortening of 3＇ UTR and the decay from the 5＇ end were confirmed by 3＇ RACE,5＇ RACE and subsequent Southern blotting.Conclusion Heterogeneous 3＇ UTR lengths render HMGA2 susceptible to different levels of negative regulation by microRNAs,which represents an important mechanism of HMGA2 reactivation in SOC.展开更多
基金supported by the projects:“Research on Classical Poetry and Cultural Identity Education for International Students in China(2023ZJY2071)”“Research on Establishing Chinese Cultural Symbols and Chinese National Images Shared by All Ethnic Groups”(ZK202410)+3 种基金Wuzhou University Education and Teaching Reform Project“Research on Innovative Teaching Mode and Evaluation of‘Poetry and Ritual Interaction’Under the Background of Skill and Emotional Deficiency(Wyjg2022A033)”“Research on Improving the Quality of Ancient Literature Curriculum Centered on Student Cognitive Ladder(Wyjg2023B016)”“Research on Innovative Integration of Information Technology and Ancient Literature Curriculum Under the Goal of New Literature Construction(Wyjg2024A018)”2022 Chinese Ancient Literature Course Ideological and Political Selection Project of Wuzhou University.
文摘This essay endeavors to dispel the lingering misconception that Li Bai,the celebrated Tang Dynasty poet,was a man devoid of propriety.Through a meticulous examination of historical records,literary works,and contemporary interpretations,this study presents a nuanced portrait of Li Bai as a poet who,despite his eccentricities and wanderlust,possessed a profound sense of etiquette and respect for societal norms.This essay argues that his unique personality in life and free expression in poetry are all driven by the need for artistic creation,rather than challenging for ancient propriety.
文摘Background Oncofetal protein high-mobility-group AT-hook protein 2 （HMGA2） is reactivated in serous ovarian cancer （SOC） and its overexpression correlates with poor prognosis.To explore the mechanism,we investigated whether HMGA2 could avoid microRNA regulation due to gene truncation or 3＇ UTR shortening by alternative polyadenylation.Methods Real-time reverse transcription polymerase chain reaction （RT-PCR） was used to evaluate the abundance of different regions of HMGA2 mRNA in 46 SOC samples.Rapid amplification of cDNA 3＇ ends （3＇ RACE） and Southern blotting were used to confirm the shortening of 3＇ untranslated region （UTR）.5＇ RACE and Southern blotting were used to prove the mRNA decay.Results No significant difference in the ratio of the stable coding region to the fragile region was observed between SOC and control normal fallopian tubes,indicating that the HMGA2 gene is not truncated in SOC.Varying degrees of 3＇ UTR shortening in SOC samples were observed by comparing the abundance of the proximal region and distal region of the HMGA2 3＇ UTR.The ratio of the proximal to the distal region of the 3＇ UTR correlated significantly with expression of the HMGA2 coding region in SOC （r=0.579,P ＜0.01）.Moreover,although the abundance of the HMGA2 coding region varied,all samples,including the very low expressed samples,exhibit relatively high levels of the proximal 3＇ UTR region,suggesting a dynamic decay of HMGA2 mRNA from the 5＇ end.The shortening of 3＇ UTR and the decay from the 5＇ end were confirmed by 3＇ RACE,5＇ RACE and subsequent Southern blotting.Conclusion Heterogeneous 3＇ UTR lengths render HMGA2 susceptible to different levels of negative regulation by microRNAs,which represents an important mechanism of HMGA2 reactivation in SOC.
