A large human naive single chain antibody (scFv) library is constructed from 60 healthy donors via phage display technique. During the period, some methods are employed to optimize the diversity, such as multi donors,...A large human naive single chain antibody (scFv) library is constructed from 60 healthy donors via phage display technique. During the period, some methods are employed to optimize the diversity, such as multi donors, different annealing temperature, half nest PCR, and assembly by two way fusion PCR. In this study, 78 electroporations resulted in 1010 library, diversity of which is assayed by enzyme fingerprint. The efficiency and diversity are all better than other researches.展开更多
个人护理品(personal care products,PCPs)是属于药物及个人护理品(pharmaceuticals and personal care products,PPCPs)的一类新生污染物质。大量研究表明,该类物质对生态环境和人类健康会产生不利影响。对环境中PCPs残留进行生态风险...个人护理品(personal care products,PCPs)是属于药物及个人护理品(pharmaceuticals and personal care products,PPCPs)的一类新生污染物质。大量研究表明,该类物质对生态环境和人类健康会产生不利影响。对环境中PCPs残留进行生态风险评价已成为该领域的研究重点。我国PCPs的环境污染状况不容乐观,而其生态风险评价方面的研究显得极为薄弱。该文就环境中PCPs研究现状以及国外生态风险评价研究进展进行了综合分析和讨论,为建立适合我国PCPs污染特点的生态风险评价体系提供重要参考。展开更多
Situs inversus viscerum(SIV)is a rare congenitalcondition characterized by complete transposition of all viscera.This anatomical pathology makes endoscopic retrograde cholangiopancreatography(ERCP)technically difficul...Situs inversus viscerum(SIV)is a rare congenitalcondition characterized by complete transposition of all viscera.This anatomical pathology makes endoscopic retrograde cholangiopancreatography(ERCP)technically difficult.We report a new case of a 70-year-old Chinese male with total SIV who had obstructive jaundice.Magneticresonance cholangiopancreatography demonstrated a number of stones in the gallbladder and common bile duct(CBD).Therapeutic ERCP was performed to relieve biliary obstruction and remove the CBD stones.This procedure started with the patient in a supine position and the endoscopist at the left side of the table.When the papilla was maintained,the patient was repositioned to a prone position and standard endoscopic sphincterotomy and endoscopic papillary balloon dilatation procedures were conducted.ERCP was performed successfully and relevant complications did not occur in this patient.We also present a review of the literature published between 1985 and 2014in the Pub Med and EMBASE databases.There were eight published cases during this period,with one each from America,Finland,India,Italy,South Korea and Pakistan,and two from Spain.Our case is the first reported in China.展开更多
The effects of BCG PSN on T cell subsets and cytokines in vernal conjunctivitis were observed. The level of total IgE was quantitatively determined before and after treatment with BCG PSN by allergen diagnostic ins...The effects of BCG PSN on T cell subsets and cytokines in vernal conjunctivitis were observed. The level of total IgE was quantitatively determined before and after treatment with BCG PSN by allergen diagnostic instrument in vitro . The content of T cell subsets of peripheral blood and cytokine were determined by using indirect immune fluorescence method, and IL 4 and INF γ were quantified by ELISA. The results showed that the level of total IgE was substantially reduced ( P <0.01) after treatment in the BCG PSN group. Meanwhile, CD + 8 was decreased, CD + 4 and CD + 4/CD + 8 ratio elevated with significant differences ( P <0.05) as compared with pre treatment results. The changes in total IgE, CD + 8 ,CD + 4 and CD + 4/CD + 8 ratio after treatment also presented significant differences ( P <0.05) between BCG PSN group and routine treatment group. The level of IL 4 in serum declined ( P <0.05) after treatment in the BCG PSN group, and INF γ went up ( P <0 05). IL 4 and INF γ in serum showed significant differences ( P <0.05) between two groups after treatment. It is concluded that BCG PSN has a bi directional immunoregulating effect. It can bring CD + 4 and CD + 8 into homeostasis, thereby preventing the occurrence of anaphylaxis. At the same time, BCG PSN can restrain Th 2, decrease the synthesis of IL 4, switch the balance of Th l/Th 2 to Th 1 side, boost up the predominance of Th 1 relatively, which is propitious to perennial stabilization and recovery of vernal conjunctivitis.展开更多
AIM: To assess the safety and feasibility of peroral esophageal myotomy(POEM) in patients with achalasia.METHODS: From January 2012 to March 2014, 50 patients(28 men, 22 women; mean age: 42.8 years, range: 14-70 years...AIM: To assess the safety and feasibility of peroral esophageal myotomy(POEM) in patients with achalasia.METHODS: From January 2012 to March 2014, 50 patients(28 men, 22 women; mean age: 42.8 years, range: 14-70 years) underwent POEM. Pre- and postoperative symptoms were quantified using the Eckardt scoring system. Barium swallow and esophagogastroscopy were performed before and after POEM, respectively. Esophageal motility was evaluated in all patients, both preoperatively and one month after POEM treatment, using a high-resolution manometry system. Manometry data, Eckardt scores, lower esophageal sphincter pressure and barium swallow results were used to evaluate the effect of the procedure.RESULTS: POEM was successfully completed for all patients. The mean procedure time was 55.4 ± 17.3 min and the mean total length of myotomy of the circular esophagus was 10.5 ± 2.6 cm. No specific complications occurred, with the exception of two patients that developed as ymptomatic pneumomediastinum and subcutaneous emphysema. Clinical improvement in symptoms was achieved in all patients. Approximately 77.5% of patients experienced weight gain 6 mo after POEM, with an average of 4.78 kg(range: 2-15 kg). The lower esophageal sphincter resting pressure, four second integrated relaxation pressure and Eckardt scores were all significantly reduced after POEM(Ps < 0.05). A small segment of proximal esophageal peristalsis appeared postoperatively in two patients, but without normal esophageal peristalsis. The average diameter of the esophageal lumen decreased significantly from 4.39 to 3.09 cm(P < 0.01). CONCLUSION: POEM can relieve achalasia symptoms, improve gastroesophageal junction relaxation and restore esophageal body motility function, but not normal esophageal peristalsis.展开更多
We identified a novel gene ST13 from a subtraetive eDNA library of normal intestinal mueosa in 1993, more studies showed that ST13 was a co-chaperone of Hsp70s. Recently we detected the ST13 gene expression in tumor t...We identified a novel gene ST13 from a subtraetive eDNA library of normal intestinal mueosa in 1993, more studies showed that ST13 was a co-chaperone of Hsp70s. Recently we detected the ST13 gene expression in tumor tissue and adjacent normal tissue of the same colorectal cancer patient and investigated if the ST13 gene expression might have any prognostic value. Analysis was performed at molecular level by reverse transcription-PCR using real-time detection method. We measured two genes simultaneously, ST13 as the target gene and glyceraldehydes-3-phosphate dehydrogenase as a reference gene, in primary colorectal tumor specimens and tumor-adjacent normal mucosa specimens from 50 colorectal cancer patients. The expression levels of the ST13 gene were significantly decreased in primary tumors compared with adjacent mucosa （P〈0.05）. But there were no significant differences in the expression of ST13 as compared depth, lymph node metastasis and disease-specific survival. with different Dukes＇ stage, tumor differentiation grade, invasion展开更多
Summary: The efficiency of cold storage red blood cells (CSRBC) or whole blood at -80 ℃ used in 27 Rh(D) negative patients during surgical operation was reported. The Rh(D) negative patients received the transfusion...Summary: The efficiency of cold storage red blood cells (CSRBC) or whole blood at -80 ℃ used in 27 Rh(D) negative patients during surgical operation was reported. The Rh(D) negative patients received the transfusion of CSRBC or whole blood stored at -80 ℃ for 180 to 360 days. The changes in the indexes, such as blood TB, DB, K +, Na +, BUN, Cr, urine protein (URPO), UOB, Hb, HCT, serum total protein, relative to hemolytic reaction and blood volume before and after transfusion were observed. The results showed that after transfusion of CSRBC or whole blood 27 cases were negative for urine protein and UOB, and the levels of BUN and Cr were normal (P>0.05). Blood TB, DB, Hb, and HCT were increased, while pH, blood K + and blood Na + was normal with the difference being not significant before and after operation (P>0.05). Plasma protein was decreased, but there was no significant difference before and after operation (P>0.05). It was suggested that CSRBC or whole blood at -80 ℃ could be safely infused to the Rh(D) negative patients without side effects during the surgical operation.展开更多
Entering 21st century, knowledge has already become the most important strategic resource for enterprises. The key factors of keeping continuous competitive advantages for an enterprise are the abilities of knowledge ...Entering 21st century, knowledge has already become the most important strategic resource for enterprises. The key factors of keeping continuous competitive advantages for an enterprise are the abilities of knowledge management and knowledge innovations. Enterprise knowledge management is a new subject with great theoretical meaning and actual meaning. From a new angle, guided by systematic viewpoint, this paper analyses deeply and explores synthetically enterprise knowledge management as a complicated system problem by doing the following:Defining the implication of enterprise knowledge management;Analyzing three kinds of the key elements in enterprise knowledge management and the interaction relations among the elements;Proposing and probing into the structure of enterprise knowledge management. Considers the structure as the open dynamic network structure with multidimensional space. Through drawing the state chart of key elements, analyzes the functions of enterprise knowledge management; Proposing and studying the interaction mechanisms between three kinds of the key elements and enterprise knowledge management. On the basis of all above analyses, aiming at the conditions and problems of enterprise knowledge management in our country, this paper probes into the main countermeasures of knowledge management that should be adopted by the enterprises of our country.展开更多
The supercritical antisolvent （SAS） process has been developed in recent years for the tormation of nanoand micro-particles. It is necessary to study the liquid phase volume expansion （LPVE） and find the relations...The supercritical antisolvent （SAS） process has been developed in recent years for the tormation of nanoand micro-particles. It is necessary to study the liquid phase volume expansion （LPVE） and find the relationships between the operating conditions and the LPVE in order to develop a practical method for determining the operation conditions and selecting an organic solvent for SAS process. The PR equation of state with vdW-1 mixing rule is used to calculate the LPVE for CO2/toluene, CO2/acetone and CO2/ethyl acetate systems, and the results show that the LPVE for each CO2/organic solvent system decreases as the temperature increases. The relationship between the LPVE and the solubility of CO2 in the liquid phase for CO2/organic solvent systems is investigated, and the results show that the LPVE is determined directly by the solubility of CO2 in the liquid phase, xCO2, and can be related to xCO2 independently. No matter what system of CO2/organic solvent is and how different the temperature is, the LPVEs have little difference as long as the solubility of CO2 in the liquid phase, xCO2, keeps constant. The lower temperature is always favorable to the SAS process. The higher the solubility of CO2 in an organic solvent under certain operation condition, the more suitable it is to the SAS process.展开更多
A new method of detecting the vertical bearing capacity for single-pile with high strain is discussed in this paper. A heavy hammer or a small type of rocket is used to strike the pile top and the detectors are used ...A new method of detecting the vertical bearing capacity for single-pile with high strain is discussed in this paper. A heavy hammer or a small type of rocket is used to strike the pile top and the detectors are used to record vibra- tion graphs. An expression of higher degree of strain (deformation force) is introduced. It is testified theoretically that the displacement, velocity and acceleration cannot be obtained by simple integral acceleration and differential velocity when long displacement and high strain exist, namely when the pile phase generates a whole slip relative to the soil body. That is to say that there are non-linear relations between them. It is educed accordingly that the force P and displacement S are calculated from the amplitude of wave train and (dynamic) P-S curve is drew so as to determine the yield points. Further, a method of determining the vertical bearing capacity for single-pile is dis- cussed. A static load test is utilized to check the result of dynamic test and determine the correlative constants of dynamic-static P(Q)-S curve.展开更多
The relationship between Compton scattered photon counts per unit mass and the content of a component in a binary powder mixture is deduced based on the Compton scattering theory.This relationship can be regarded line...The relationship between Compton scattered photon counts per unit mass and the content of a component in a binary powder mixture is deduced based on the Compton scattering theory.This relationship can be regarded linear after some approximations.The scattered photon counts per unit mass increases faster with the content when the difference of the atomic numbers of the two components in the binary powder mixture is larger.The relationship was verified with Compton scattering experiments on a series of Ni-Cu and Fe-C binary powder mixtures.The method of using this linear dependene of scattered photon counts on the content of a binary powder mixture to ascertain the component content in a binary mixture was introduced.展开更多
With the rapid development of microelectronics and hardware,the use of ever faster micro processors and new architecture must be continued to meet tomorrow′s computing needs. New processor microarchitectures are need...With the rapid development of microelectronics and hardware,the use of ever faster micro processors and new architecture must be continued to meet tomorrow′s computing needs. New processor microarchitectures are needed to push performance further and to use higher transistor counts effectively.At the same time,aiming at different usages,the processor has been optimized in different aspects,such as high performace,low power consumption,small chip area and high security. SOC (System on chip)and SCMP (Single Chip Multi Processor) constitute the main processor system architecture.展开更多
文摘A large human naive single chain antibody (scFv) library is constructed from 60 healthy donors via phage display technique. During the period, some methods are employed to optimize the diversity, such as multi donors, different annealing temperature, half nest PCR, and assembly by two way fusion PCR. In this study, 78 electroporations resulted in 1010 library, diversity of which is assayed by enzyme fingerprint. The efficiency and diversity are all better than other researches.
文摘个人护理品(personal care products,PCPs)是属于药物及个人护理品(pharmaceuticals and personal care products,PPCPs)的一类新生污染物质。大量研究表明,该类物质对生态环境和人类健康会产生不利影响。对环境中PCPs残留进行生态风险评价已成为该领域的研究重点。我国PCPs的环境污染状况不容乐观,而其生态风险评价方面的研究显得极为薄弱。该文就环境中PCPs研究现状以及国外生态风险评价研究进展进行了综合分析和讨论,为建立适合我国PCPs污染特点的生态风险评价体系提供重要参考。
文摘Situs inversus viscerum(SIV)is a rare congenitalcondition characterized by complete transposition of all viscera.This anatomical pathology makes endoscopic retrograde cholangiopancreatography(ERCP)technically difficult.We report a new case of a 70-year-old Chinese male with total SIV who had obstructive jaundice.Magneticresonance cholangiopancreatography demonstrated a number of stones in the gallbladder and common bile duct(CBD).Therapeutic ERCP was performed to relieve biliary obstruction and remove the CBD stones.This procedure started with the patient in a supine position and the endoscopist at the left side of the table.When the papilla was maintained,the patient was repositioned to a prone position and standard endoscopic sphincterotomy and endoscopic papillary balloon dilatation procedures were conducted.ERCP was performed successfully and relevant complications did not occur in this patient.We also present a review of the literature published between 1985 and 2014in the Pub Med and EMBASE databases.There were eight published cases during this period,with one each from America,Finland,India,Italy,South Korea and Pakistan,and two from Spain.Our case is the first reported in China.
文摘The effects of BCG PSN on T cell subsets and cytokines in vernal conjunctivitis were observed. The level of total IgE was quantitatively determined before and after treatment with BCG PSN by allergen diagnostic instrument in vitro . The content of T cell subsets of peripheral blood and cytokine were determined by using indirect immune fluorescence method, and IL 4 and INF γ were quantified by ELISA. The results showed that the level of total IgE was substantially reduced ( P <0.01) after treatment in the BCG PSN group. Meanwhile, CD + 8 was decreased, CD + 4 and CD + 4/CD + 8 ratio elevated with significant differences ( P <0.05) as compared with pre treatment results. The changes in total IgE, CD + 8 ,CD + 4 and CD + 4/CD + 8 ratio after treatment also presented significant differences ( P <0.05) between BCG PSN group and routine treatment group. The level of IL 4 in serum declined ( P <0.05) after treatment in the BCG PSN group, and INF γ went up ( P <0 05). IL 4 and INF γ in serum showed significant differences ( P <0.05) between two groups after treatment. It is concluded that BCG PSN has a bi directional immunoregulating effect. It can bring CD + 4 and CD + 8 into homeostasis, thereby preventing the occurrence of anaphylaxis. At the same time, BCG PSN can restrain Th 2, decrease the synthesis of IL 4, switch the balance of Th l/Th 2 to Th 1 side, boost up the predominance of Th 1 relatively, which is propitious to perennial stabilization and recovery of vernal conjunctivitis.
文摘AIM: To assess the safety and feasibility of peroral esophageal myotomy(POEM) in patients with achalasia.METHODS: From January 2012 to March 2014, 50 patients(28 men, 22 women; mean age: 42.8 years, range: 14-70 years) underwent POEM. Pre- and postoperative symptoms were quantified using the Eckardt scoring system. Barium swallow and esophagogastroscopy were performed before and after POEM, respectively. Esophageal motility was evaluated in all patients, both preoperatively and one month after POEM treatment, using a high-resolution manometry system. Manometry data, Eckardt scores, lower esophageal sphincter pressure and barium swallow results were used to evaluate the effect of the procedure.RESULTS: POEM was successfully completed for all patients. The mean procedure time was 55.4 ± 17.3 min and the mean total length of myotomy of the circular esophagus was 10.5 ± 2.6 cm. No specific complications occurred, with the exception of two patients that developed as ymptomatic pneumomediastinum and subcutaneous emphysema. Clinical improvement in symptoms was achieved in all patients. Approximately 77.5% of patients experienced weight gain 6 mo after POEM, with an average of 4.78 kg(range: 2-15 kg). The lower esophageal sphincter resting pressure, four second integrated relaxation pressure and Eckardt scores were all significantly reduced after POEM(Ps < 0.05). A small segment of proximal esophageal peristalsis appeared postoperatively in two patients, but without normal esophageal peristalsis. The average diameter of the esophageal lumen decreased significantly from 4.39 to 3.09 cm(P < 0.01). CONCLUSION: POEM can relieve achalasia symptoms, improve gastroesophageal junction relaxation and restore esophageal body motility function, but not normal esophageal peristalsis.
文摘We identified a novel gene ST13 from a subtraetive eDNA library of normal intestinal mueosa in 1993, more studies showed that ST13 was a co-chaperone of Hsp70s. Recently we detected the ST13 gene expression in tumor tissue and adjacent normal tissue of the same colorectal cancer patient and investigated if the ST13 gene expression might have any prognostic value. Analysis was performed at molecular level by reverse transcription-PCR using real-time detection method. We measured two genes simultaneously, ST13 as the target gene and glyceraldehydes-3-phosphate dehydrogenase as a reference gene, in primary colorectal tumor specimens and tumor-adjacent normal mucosa specimens from 50 colorectal cancer patients. The expression levels of the ST13 gene were significantly decreased in primary tumors compared with adjacent mucosa （P〈0.05）. But there were no significant differences in the expression of ST13 as compared depth, lymph node metastasis and disease-specific survival. with different Dukes＇ stage, tumor differentiation grade, invasion
文摘Summary: The efficiency of cold storage red blood cells (CSRBC) or whole blood at -80 ℃ used in 27 Rh(D) negative patients during surgical operation was reported. The Rh(D) negative patients received the transfusion of CSRBC or whole blood stored at -80 ℃ for 180 to 360 days. The changes in the indexes, such as blood TB, DB, K +, Na +, BUN, Cr, urine protein (URPO), UOB, Hb, HCT, serum total protein, relative to hemolytic reaction and blood volume before and after transfusion were observed. The results showed that after transfusion of CSRBC or whole blood 27 cases were negative for urine protein and UOB, and the levels of BUN and Cr were normal (P>0.05). Blood TB, DB, Hb, and HCT were increased, while pH, blood K + and blood Na + was normal with the difference being not significant before and after operation (P>0.05). Plasma protein was decreased, but there was no significant difference before and after operation (P>0.05). It was suggested that CSRBC or whole blood at -80 ℃ could be safely infused to the Rh(D) negative patients without side effects during the surgical operation.
文摘Entering 21st century, knowledge has already become the most important strategic resource for enterprises. The key factors of keeping continuous competitive advantages for an enterprise are the abilities of knowledge management and knowledge innovations. Enterprise knowledge management is a new subject with great theoretical meaning and actual meaning. From a new angle, guided by systematic viewpoint, this paper analyses deeply and explores synthetically enterprise knowledge management as a complicated system problem by doing the following:Defining the implication of enterprise knowledge management;Analyzing three kinds of the key elements in enterprise knowledge management and the interaction relations among the elements;Proposing and probing into the structure of enterprise knowledge management. Considers the structure as the open dynamic network structure with multidimensional space. Through drawing the state chart of key elements, analyzes the functions of enterprise knowledge management; Proposing and studying the interaction mechanisms between three kinds of the key elements and enterprise knowledge management. On the basis of all above analyses, aiming at the conditions and problems of enterprise knowledge management in our country, this paper probes into the main countermeasures of knowledge management that should be adopted by the enterprises of our country.
基金Supported by the National Nature Science Foundation of China (No. 20176003)
文摘The supercritical antisolvent （SAS） process has been developed in recent years for the tormation of nanoand micro-particles. It is necessary to study the liquid phase volume expansion （LPVE） and find the relationships between the operating conditions and the LPVE in order to develop a practical method for determining the operation conditions and selecting an organic solvent for SAS process. The PR equation of state with vdW-1 mixing rule is used to calculate the LPVE for CO2/toluene, CO2/acetone and CO2/ethyl acetate systems, and the results show that the LPVE for each CO2/organic solvent system decreases as the temperature increases. The relationship between the LPVE and the solubility of CO2 in the liquid phase for CO2/organic solvent systems is investigated, and the results show that the LPVE is determined directly by the solubility of CO2 in the liquid phase, xCO2, and can be related to xCO2 independently. No matter what system of CO2/organic solvent is and how different the temperature is, the LPVEs have little difference as long as the solubility of CO2 in the liquid phase, xCO2, keeps constant. The lower temperature is always favorable to the SAS process. The higher the solubility of CO2 in an organic solvent under certain operation condition, the more suitable it is to the SAS process.
文摘A new method of detecting the vertical bearing capacity for single-pile with high strain is discussed in this paper. A heavy hammer or a small type of rocket is used to strike the pile top and the detectors are used to record vibra- tion graphs. An expression of higher degree of strain (deformation force) is introduced. It is testified theoretically that the displacement, velocity and acceleration cannot be obtained by simple integral acceleration and differential velocity when long displacement and high strain exist, namely when the pile phase generates a whole slip relative to the soil body. That is to say that there are non-linear relations between them. It is educed accordingly that the force P and displacement S are calculated from the amplitude of wave train and (dynamic) P-S curve is drew so as to determine the yield points. Further, a method of determining the vertical bearing capacity for single-pile is dis- cussed. A static load test is utilized to check the result of dynamic test and determine the correlative constants of dynamic-static P(Q)-S curve.
基金Funded by the Basic Research of Chongqing Municipal Education Commission(Grant No.KJ080825)Natural Science Research Fund for the Doctors of Chongqing Normal University(Grant No.08XLB015)
文摘The relationship between Compton scattered photon counts per unit mass and the content of a component in a binary powder mixture is deduced based on the Compton scattering theory.This relationship can be regarded linear after some approximations.The scattered photon counts per unit mass increases faster with the content when the difference of the atomic numbers of the two components in the binary powder mixture is larger.The relationship was verified with Compton scattering experiments on a series of Ni-Cu and Fe-C binary powder mixtures.The method of using this linear dependene of scattered photon counts on the content of a binary powder mixture to ascertain the component content in a binary mixture was introduced.
文摘With the rapid development of microelectronics and hardware,the use of ever faster micro processors and new architecture must be continued to meet tomorrow′s computing needs. New processor microarchitectures are needed to push performance further and to use higher transistor counts effectively.At the same time,aiming at different usages,the processor has been optimized in different aspects,such as high performace,low power consumption,small chip area and high security. SOC (System on chip)and SCMP (Single Chip Multi Processor) constitute the main processor system architecture.
