目的利用360°全方向24和36声源测试设备,初步探讨健听中青年和健听老年前期-老年人水平声源定位特点。方法选取2021年4月至2021年9月中国人民解放军总医院耳鼻喉科收治的43例健听成年受试者为研究对象,其中男性22例,女性21例;根据...目的利用360°全方向24和36声源测试设备,初步探讨健听中青年和健听老年前期-老年人水平声源定位特点。方法选取2021年4月至2021年9月中国人民解放军总医院耳鼻喉科收治的43例健听成年受试者为研究对象,其中男性22例,女性21例;根据年龄分为中青年组(21~49岁)20例和老年前期-老年组(50~72岁)23例。两组分别给予纯音听阈测试、全方向24声源(间隔15°)和36声源(间隔10°)水平声源定位(sound localization,SL)能力评估。给声强度60 dB HL,给声刺激为1 kHz啭音,通过计算均方根误差(root mean square,RMS)、平均绝对误差(mean absolutely error,MAE)等评估受试者的声源定位能力。结果24声源老年前期-老年组MAE、RMS均值高于中青年组的MAE、RMS均值,差异有统计学意义(P<0.05);36声源老年前期-老年组MAE、RMS高于中青年组的MAE、RMS,差异无统计学意义(P>0.05)。24声源和36声源前场MAE和RMS均高于后场的MAE和RMS,前后场的MAE和RMS比较,差异有统计学意义(P<0.01);左右场的MAE、RMS比较,差异无统计学意义(P>0.05)。24声源前后混淆比例为7.73%,36声源前后混淆比例为15.42%;24声源和36声源均为正前方的声源定位准确度最差;老年前期-老年组前后混淆的比例高于中青年组,差异无统计学意义(P>0.05)。结论健听老年前期-老年人全方向24声源和36声源水平定位能力,相比健听中青年组有所下降。左右场的定位准确度高,前后场的定位准确度低,正前方定位准确度最低。全方向水平声源定位能力的测试结果与扬声器数量有关,且反应趋势具有一致性。展开更多
Background Plant hormones profoundly influence cotton growth,development,and responses to various stresses.Therefore,there is a pressing need for an efficient assay to quantify these hormones in cotton.In this groundb...Background Plant hormones profoundly influence cotton growth,development,and responses to various stresses.Therefore,there is a pressing need for an efficient assay to quantify these hormones in cotton.In this groundbreaking study,we have established QuEChERS-HPLC‒MS/MS method,for the simultaneous detection of multiple plant hormones in cotton leaves,allowing the analysis and quantification of five key plant hormones.Results Sample extraction and purification employed 0.1%acetic acid in methanol and C18 for optimal recovery of plant hormones.The method applied to cotton demonstrated excellent linearity across a concentration range of 0.05–1 mg・L−1,with linear regression coefficients exceeding 0.99.The limits of quantification(LOQs)were 20μg・kg−1 for GA3 and 5μg・kg−1 for the other four plant hormones.Recovery rates for the five plant hormones matrix spiked at levels of 5,10,100,and 1000μg・kg−1 were in the range of 79.07%to 98.97%,with intraday relative standard deviations(RSDs)ranging from 2.11%to 8.47%.The method was successfully employed to analyze and quantify the five analytes in cotton leaves treated with plant growth regulators.Conclusion The study demonstrates that the method is well-suited for the determination of five plant hormones in cotton.It exhibits excellent selectivity and sensitivity in detecting field samples,thus serving as a robust tool for indepth research into cotton physiology.展开更多
文摘目的利用360°全方向24和36声源测试设备,初步探讨健听中青年和健听老年前期-老年人水平声源定位特点。方法选取2021年4月至2021年9月中国人民解放军总医院耳鼻喉科收治的43例健听成年受试者为研究对象,其中男性22例,女性21例;根据年龄分为中青年组(21~49岁)20例和老年前期-老年组(50~72岁)23例。两组分别给予纯音听阈测试、全方向24声源(间隔15°)和36声源(间隔10°)水平声源定位(sound localization,SL)能力评估。给声强度60 dB HL,给声刺激为1 kHz啭音,通过计算均方根误差(root mean square,RMS)、平均绝对误差(mean absolutely error,MAE)等评估受试者的声源定位能力。结果24声源老年前期-老年组MAE、RMS均值高于中青年组的MAE、RMS均值,差异有统计学意义(P<0.05);36声源老年前期-老年组MAE、RMS高于中青年组的MAE、RMS,差异无统计学意义(P>0.05)。24声源和36声源前场MAE和RMS均高于后场的MAE和RMS,前后场的MAE和RMS比较,差异有统计学意义(P<0.01);左右场的MAE、RMS比较,差异无统计学意义(P>0.05)。24声源前后混淆比例为7.73%,36声源前后混淆比例为15.42%;24声源和36声源均为正前方的声源定位准确度最差;老年前期-老年组前后混淆的比例高于中青年组,差异无统计学意义(P>0.05)。结论健听老年前期-老年人全方向24声源和36声源水平定位能力,相比健听中青年组有所下降。左右场的定位准确度高,前后场的定位准确度低,正前方定位准确度最低。全方向水平声源定位能力的测试结果与扬声器数量有关,且反应趋势具有一致性。
基金National Key R&D Program of China(2022YFD1400300)Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural SciencesChina Agriculture Research System.
文摘Background Plant hormones profoundly influence cotton growth,development,and responses to various stresses.Therefore,there is a pressing need for an efficient assay to quantify these hormones in cotton.In this groundbreaking study,we have established QuEChERS-HPLC‒MS/MS method,for the simultaneous detection of multiple plant hormones in cotton leaves,allowing the analysis and quantification of five key plant hormones.Results Sample extraction and purification employed 0.1%acetic acid in methanol and C18 for optimal recovery of plant hormones.The method applied to cotton demonstrated excellent linearity across a concentration range of 0.05–1 mg・L−1,with linear regression coefficients exceeding 0.99.The limits of quantification(LOQs)were 20μg・kg−1 for GA3 and 5μg・kg−1 for the other four plant hormones.Recovery rates for the five plant hormones matrix spiked at levels of 5,10,100,and 1000μg・kg−1 were in the range of 79.07%to 98.97%,with intraday relative standard deviations(RSDs)ranging from 2.11%to 8.47%.The method was successfully employed to analyze and quantify the five analytes in cotton leaves treated with plant growth regulators.Conclusion The study demonstrates that the method is well-suited for the determination of five plant hormones in cotton.It exhibits excellent selectivity and sensitivity in detecting field samples,thus serving as a robust tool for indepth research into cotton physiology.
