Objective:This study aims to analyze how changes in pathological diagnosis practice and molecular detection technology have affected clinical outcomes for colorectal cancer(CRC)patients in Fudan University Shanghai Ca...Objective:This study aims to analyze how changes in pathological diagnosis practice and molecular detection technology have affected clinical outcomes for colorectal cancer(CRC)patients in Fudan University Shanghai Cancer Center(FUSCC).Methods:This retrospective cohort study analyzed 21,141 pathologically confirmed CRC cases diagnosed at FUSCC from 2008 to 2020.Patients were divided into five groups for different analytical purposes:(1)the before vs.since 2014 groups to analyze the influence of the changes in the classification criteria of pT3 and pT4 staging on the survival of patients;(2)the partial vs.total mesorectal excision(TME)groups to analyze whether evaluation of completeness of the mesorectum have impact on the survival of patients;(3)the tumor deposit(TD)(+)N0 vs.TD(+)N1c groups to analyze the influence of the changes in the pN staging on the survival of patients with positive TD and negative regional lymph node metastasis(LNM);(4)the before vs.since 2013 groups to analyze the influence of the changes in the testing process of deficient mismatch repair on the survival of patients;and(5)the groups with vs.without RAS/BRAF gene mutation testing to analyze the influence of these testing on the survival of patients.Patients’clinicopathological parameters,including age at diagnosis,sex,tumor size,location,differentiation,mucinous subtype,TD,lymphovascular invasion,perineural invasion,tumor depth,LNM and distant metastasis,and tumor-node-metastasis(TNM)stage,were compared between groups.Kaplan-Meier analysis with log rank method was performed for patients’overall survival(OS)and disease-free survival(DFS)analyses.Results:In pathological reports,there were three parameter changes that impacted patient outcomes.Firstly,changes in the pT staging criteria led to a shift of the ratio of patients with stage pT3 to stage pT4 from 1:110.9 to 1:0.26.In comparison to patients admitted before 2014(n=4,754),a significant difference in prognosis between pT3 and pT4 stages was observed since 2014(n=9,965).Secondly,we began to evaluate the completeness of the mesorectum since 2016.As a result,91.0%of patients with low rectal cancer underwent TME(n=4,111)surgery,and patients with TME had significantly better OS compared with partial mesorectal excision(PME,n=409).Thirdly,we began to stage TD(+)LNM(-)as N1c since 2017.The results showed that N1c(n=127)but not N0(n=39)can improve the prognosis of patients without LNM and distal metastasis.In molecular testing,there have been three and five iterations of updates regarding mismatch repair(MMR)/microsatellite instability(MSI)status and RAS/BRAF gene mutation detection,respectively.The standardization of MMR status testing has sharply decreased the proportion of deficient MMR(dMMR)patients(from 32.5%to 7.4%)since 2013.The prognosis of patients underwent MMR status testing since 2013(n=867)were significantly better than patients before 2013(n=1,313).In addition,detection of RAS/BRAF gene mutation status(n=5,041)resulted in better DFS but not OS,for patients with stage I-III disease(n=16,557).Conclusion:Over the past few decades,updates in elements in pathological reports,as well as the development of standardized tests for MMR/MSI status and RAS/BRAF gene mutations have significantly improved patient outcomes.展开更多
X-ray observations play a crucial role in time-domain astronomy.The Einstein Probe(EP),a recently launched X-ray astronomical satellite,emerges as a forefront player in the field of time-domain astronomy and high-ener...X-ray observations play a crucial role in time-domain astronomy.The Einstein Probe(EP),a recently launched X-ray astronomical satellite,emerges as a forefront player in the field of time-domain astronomy and high-energy astrophysics.With a focus on systematic surveys in the soft X-ray band,EP aims to discover high-energy transients and monitor variable sources in the universe.To achieve these objectives,a quick and reliable classification of observed sources is essential.In this study,we developed a machine learning classifier for autonomous source classification using data from the EP-WXT Pathfinder—Lobster Eye Imager for Astronomy(LEIA)and EP-WXT simulations.The proposed Random Forest classifier,built on selected features derived from light curves,energy spectra,and location information,achieves an accuracy of approximately 95%on EP simulation data and 98%on LEIA observational data.The classifier is integrated into the LEIA data processing pipeline,serving as a tool for manual validation and rapid classification during observations.This paper presents an efficient method for the classification of X-ray sources based on single observations,along with implications of most effective features for the task.This work facilitates rapid source classification for the EP mission and also provides valuable insights into feature selection and classification techniques for enhancing the efficiency and accuracy of X-ray source classification that can be adapted to other X-ray telescope data.展开更多
The evaluation of the electricity market is crucial for fostering market construction and development.An accurate assessment of the electricity market reveals developmental trends,identifies operational issues,and con...The evaluation of the electricity market is crucial for fostering market construction and development.An accurate assessment of the electricity market reveals developmental trends,identifies operational issues,and contributes to stable and healthy market growth.This study investigated the characteristics of electricity markets in different provinces and synthesized a comprehensive set of evaluation indicators to assess market effectiveness.The evaluation framework,comprising nine indicators organized into two tiers,was constructed based on three aspects:market design,market efficiency,and developmental coordination.Furthermore,a novel fuzzy multi-criteria decision-making evaluation model for electricity market performance was developed based on the Fuzzy-BWM and fuzzy COPRAS methodologies.This model aimed to ensure both accuracy and comprehensiveness in market operation assessment.Subsequently,empirical analyses were conducted on four typical provincial-level electricity markets in China.The results indicate that Guangdong’s electricity market performed best because of its effective balance of stakeholder interests and adherence to contractual integrity principles.Zhejiang and Shandong ranked second and third,respectively,whereas Sichuan exhibited the poorest market performance.Sichuan’s electricity market must be improved in terms of market design,such that market players can obtain a fairly competitive environment.The sensitivity analysis of the constructed indicators verified the effectiveness of the evaluation model proposed in this study.Finally,policy recommendations were proposed to facilitate the sustainable development of China’s electricity markets with the objective of transforming them into efficient and secure markets adaptable to the evolution of novel power systems.展开更多
3,3′,5-Triiodo-L-thyronine(T3)is a key endocrine hormone in the human body that plays crucial roles in growth,development,metabolism,and immune function.Macrophages,the key regulatory cells within the immune system,e...3,3′,5-Triiodo-L-thyronine(T3)is a key endocrine hormone in the human body that plays crucial roles in growth,development,metabolism,and immune function.Macrophages,the key regulatory cells within the immune system,exhibit marked“heterogeneity”and“plasticity”,with their phenotype and function subject to modulation by local environmental signals.The interplay between the endocrine and immune systems is well documented.Numerous studies have shown that T3 significantly target macrophages,highlighting them as key cellular components in this interaction.Through the regulation of macrophage function and phenotype,T3 influences immune function and tissue repair in the body.This review comprehensively summarizes the regulatory actions and mechanisms of T3 on macrophages,offering valuable insights into further research of the immunoregulatory effects of T3.展开更多
BACKGROUND Cyclic vomiting syndrome(CVS)is a chronic functional gastrointestinal disorder involving the gut–brain interaction that is characterized by recurring episodes of nausea,vomiting,abdominal pain,and interspe...BACKGROUND Cyclic vomiting syndrome(CVS)is a chronic functional gastrointestinal disorder involving the gut–brain interaction that is characterized by recurring episodes of nausea,vomiting,abdominal pain,and interspersed complete normal periods.Superior mesenteric artery(SMA)syndrome(SMAS)is a vascular condition in which the horizontal portion of the duodenum is compressed due to a reduced angle between the aorta and the SMA.This condition presents with symptoms similar to CVS,posing challenges in distinguishing between the two and often resulting in misdiagnosis or inappropriate treatment.CASE SUMMARY A 20-year-old female patient presented with recurrent episodes of vomiting and experienced a persistent fear of vomiting for the past 2 years.She adopted conscious dietary restrictions,which led to severe malnutrition.Initially,she was diagnosed with SMAS,as revealed by computed tomography angiography.Despite efforts to increase the angle between the aorta and the SMA through weight gain,her vomiting did not improve.Finally,she was diagnosed with comorbidities including CVS,SMAS and anxiety disorder.She underwent comprehensive interventions,including enteral and parenteral nutritional supplementation,administration of antiemetic and anti-anxiety agents,and participation in mindfulness-based cognitive therapy.The patient eventually experienced a notable improvement in both body weight and clinical symptoms.CONCLUSION We present a rare case of CVS in an adult complicated with SMAS and propose additional treatment with nutritional support,pharmacological intervention,and psychotherapy.展开更多
In the article‘MicroRNA-329-3p inhibits the Wnt/β-catenin pathway and proliferation of osteosarcoma cells by targeting transcription factor 7-like 1’(Oncology Research,2024,Vol.32,No.3,pp.463−476.doi:10.32604/or.20...In the article‘MicroRNA-329-3p inhibits the Wnt/β-catenin pathway and proliferation of osteosarcoma cells by targeting transcription factor 7-like 1’(Oncology Research,2024,Vol.32,No.3,pp.463−476.doi:10.32604/or.2023.044085),there was an error in the compilation of Fig.8D.We have revised Fig.8D to correct this error.A corrected version of Fig.8 is provided.This correction does not change any results or conclusions of the article.We apologize for any inconvenience caused.展开更多
BACKGROUND： This study aimed to explore the effects of TNF-α on the expression of IP3R1 mRNA and protein in human mesangial cells （HMCs）, and to elucidate the mechanism of TNF-α relating to IP3R1 expression in th...BACKGROUND： This study aimed to explore the effects of TNF-α on the expression of IP3R1 mRNA and protein in human mesangial cells （HMCs）, and to elucidate the mechanism of TNF-α relating to IP3R1 expression in the occurrence of hepatorenal syndrome （HRS）.METHODS： HMCs were stimulated by tumor （TNF-α） with 100 ng/mL for different hours （2, 4, 8, and 24 hours）. The expression changes of IP3R1 mRNA and protein were detected by quantitative real-time polymerase chain reaction and immunoblotting. Several inhibitors including D609, U73122, PP1, safingol, rottlerin and non-radioactive protein kinase C （PKC） were used to examine the mechanism of signal transduction of TNF-α-regulated IP3R1 in HMCs.RESULTS： The levels of IP3R1 mRNA at 2 hours after TNF-α exposure were significantly enhanced and peaked at 8 hours in HMCs （P〈0.01）, then descended at 24 hours （P〈0.01）. The levels of IP3R1 protein at 4 hours after TNF-α exposure were obviously increased and peaked at 24 hours after TNF-α exposure （P〈0.01）. Compared to the control group, safingol （PKCa inhibitor） and D609 （phosphatidylcholine-specific phospholipase C inhibitor） significantly blocked the TNF-α- induced expression of IP3R1 mRNA （3.30±0.81 vs. 1.95±0.13, P〈0.05; 2.10±0.49, P〈0.01） and IP3R1 protein （3.09±0.13 vs. 1.86±0.39, P〈0.01; 1.98±0.02, P〈0.01）. TNF-α promoted PKCa activation with maximal PKCa phosphorylation that occurred 8 hours after stimulation measured by non-radioactive PKC assay, and the effect was markedly attenuated by pretreatment with D609 or safingol.CONCLUSION： TNF-α increased the expression of IP3R1 and this was mediated, at least in part, through the PC-PLC/PKCa signaling pathways in HMCs.展开更多
Solitary rectal ulcer syndrome(SRUS) is a rare benign condition, which can mimic many other diseases because of their similarities in clinical, endoscopic and histological features. Sessile serrated adenoma/polyp(SSA/...Solitary rectal ulcer syndrome(SRUS) is a rare benign condition, which can mimic many other diseases because of their similarities in clinical, endoscopic and histological features. Sessile serrated adenoma/polyp(SSA/p) is a premalignant lesion in the colon and rectum. The misdiagnosis of SSA/p in SRUS patients has been noted, but the case of SRUS arising secondarily to SSA/p has been rarely reported. We herein report the case of a 59-year-old man who presented with an ulcerative nodular lesion in the rectum, accompanied by the symptoms of blood and mucus in the feces, diarrhea and constipation. Magnetic resonance imagining revealed thickening of the rectal mucosa-submucosa. Histologically, the lesion was characterized by the hyperplastic lamina propria and diffusely serrated crypts. Further immunohistochemical staining showed the loss of HES1 and MLH1 expression in the epithelial cells in the serrated area. The patient with SRUS had histological changes of SSA/p, suggesting a potential of tumor transformation in certain cases. SRUS uncommonly accompanied by serrated lesions should at least be considered by pathologists and clinicians.展开更多
Background:In recent years,day surgery has gained widespread attention in the medical industry.This thesis focuses on three tertiary general hospitals in different regions in China where day surgery has been carried o...Background:In recent years,day surgery has gained widespread attention in the medical industry.This thesis focuses on three tertiary general hospitals in different regions in China where day surgery has been carried out earlier,is more developed,has a more comprehensive range of day surgery procedures,and has an annual volume of 10,000 or more day surgery cases.The study will provide hands-on experience for other medical institutions to carry out day surgery.Methods:67,337 valid items of data were retrieved from the Hospital Information Systems of the three hospitals.In this thesis,descriptive analysis was carried out on basic information about day surgery patients,including composition of patients’major diseases,hospitalization costs.Non-parametric tests were used to compare the data in each group,the median and interquartile range were used to show the trend in the concentration of the relevant indicators.The difference was considered statistically significant at P<0.05.Results:The largest proportion of day surgery patients were between the ages of 51 and 70.About 57.30%of day surgery patients paid for their medical care with the basic medical insurance of urban employees.Senile cataract,benign breast tumor,and endometrial polyp were the top three major diagnoses of day surgery patients.The average inpatient expenditure for day surgery patients was 8982.10 yuan,with consumables cost representing the highest proportion at approximately 46.01%of the total hospitalization cost,followed by treatment cost at around 31.01%of the total cost.Conclusions:Day surgery is more suitable for middle-aged patients and may not be the best choice for children and elderly patients over 80 years old.Most day surgery cases are elective surgeries for non-emergency situations.The distribution of day surgery patients is primarily concentrated in ophthalmic,breast,and reproductive system diseases,although each hospital has its own unique characteristics.The development of day surgery presents both economic and social benefits.展开更多
Seasonally frozen soil in alpine and subalpine zones in the mountains of Qinghai-Tibetan Plateau is particularly sensitive to global climate change. Therefore, a better understanding of the thermal properties of froze...Seasonally frozen soil in alpine and subalpine zones in the mountains of Qinghai-Tibetan Plateau is particularly sensitive to global climate change. Therefore, a better understanding of the thermal properties of frozen soil is crucial for predicting the responses of frozen soils to soil warming. In this study, thermal properties of frozen soil with different moisture contents under subzero temperature (0°C - 20°C) in an alpine forest in western Sichuan were analyzed by KD<sub>2</sub> Pro in its cooling and heating processes, respectively. Our results reveal that the soil apparent volumetric specific heat capacity (C<sub>v</sub>) and apparent thermal conductivity (K) under the same water content show similar response patterns to changing temperature lower than -2°C in both heating and cooling processes. Moreover, ice content of frozen soils can be well predicted by Logistic model in cooling and heating processes. The C<sub>v</sub> and K tend to increase along with increasing soil moisture contents. Remarkably, asymptotic characters of the value of C<sub>v</sub> and K are at the vicinity of the initial temperature of phase transitions, indicating that both C<sub>v</sub> and K are particularly sensitive to changing soil temperature at the range of -2°C to 0°C. Therefore, the widely distributed frozen soil layers with temperature above -2°C in alpine and subalpine zones over Qinghai-Tibetan Plateau are susceptible to the observed climate warming during cold season.展开更多
基金supported by the National Natural Science Foundation of China(No.51627802)the Inner Mongolia Science and Technology Revitalization Project,China(No.XM2020BT14)。
基金supported by National Natural Science Foundation of China(grant numbers:82273370,82202899,82172702,81972249,81902430,82002543,82002946,U1932145)Shanghai Clinical Science and Technology Innovation Project of Municipal Hospital(grant number:SHDC12020102)+5 种基金Natural Science Foundation of Shanghai(grant numbers:22ZR1413000,21ZR1414900)Artificial Intelligence Medical Hospital Cooperation Project of Xuhui District(grant number:2021-017)Shanghai Science and Technology Development Fund(grant number:19MC1911000)Shanghai Municipal Key Clinical Specialty(grant number:shslczdzk01301)Science and Technology Commission of Shanghai Municipality(grant number:18401933402)“Chenguang Program”supported by Shanghai Education Development Foundation and Shanghai Municipal Education Commission(grant number:20CG08).
文摘Objective:This study aims to analyze how changes in pathological diagnosis practice and molecular detection technology have affected clinical outcomes for colorectal cancer(CRC)patients in Fudan University Shanghai Cancer Center(FUSCC).Methods:This retrospective cohort study analyzed 21,141 pathologically confirmed CRC cases diagnosed at FUSCC from 2008 to 2020.Patients were divided into five groups for different analytical purposes:(1)the before vs.since 2014 groups to analyze the influence of the changes in the classification criteria of pT3 and pT4 staging on the survival of patients;(2)the partial vs.total mesorectal excision(TME)groups to analyze whether evaluation of completeness of the mesorectum have impact on the survival of patients;(3)the tumor deposit(TD)(+)N0 vs.TD(+)N1c groups to analyze the influence of the changes in the pN staging on the survival of patients with positive TD and negative regional lymph node metastasis(LNM);(4)the before vs.since 2013 groups to analyze the influence of the changes in the testing process of deficient mismatch repair on the survival of patients;and(5)the groups with vs.without RAS/BRAF gene mutation testing to analyze the influence of these testing on the survival of patients.Patients’clinicopathological parameters,including age at diagnosis,sex,tumor size,location,differentiation,mucinous subtype,TD,lymphovascular invasion,perineural invasion,tumor depth,LNM and distant metastasis,and tumor-node-metastasis(TNM)stage,were compared between groups.Kaplan-Meier analysis with log rank method was performed for patients’overall survival(OS)and disease-free survival(DFS)analyses.Results:In pathological reports,there were three parameter changes that impacted patient outcomes.Firstly,changes in the pT staging criteria led to a shift of the ratio of patients with stage pT3 to stage pT4 from 1:110.9 to 1:0.26.In comparison to patients admitted before 2014(n=4,754),a significant difference in prognosis between pT3 and pT4 stages was observed since 2014(n=9,965).Secondly,we began to evaluate the completeness of the mesorectum since 2016.As a result,91.0%of patients with low rectal cancer underwent TME(n=4,111)surgery,and patients with TME had significantly better OS compared with partial mesorectal excision(PME,n=409).Thirdly,we began to stage TD(+)LNM(-)as N1c since 2017.The results showed that N1c(n=127)but not N0(n=39)can improve the prognosis of patients without LNM and distal metastasis.In molecular testing,there have been three and five iterations of updates regarding mismatch repair(MMR)/microsatellite instability(MSI)status and RAS/BRAF gene mutation detection,respectively.The standardization of MMR status testing has sharply decreased the proportion of deficient MMR(dMMR)patients(from 32.5%to 7.4%)since 2013.The prognosis of patients underwent MMR status testing since 2013(n=867)were significantly better than patients before 2013(n=1,313).In addition,detection of RAS/BRAF gene mutation status(n=5,041)resulted in better DFS but not OS,for patients with stage I-III disease(n=16,557).Conclusion:Over the past few decades,updates in elements in pathological reports,as well as the development of standardized tests for MMR/MSI status and RAS/BRAF gene mutations have significantly improved patient outcomes.
基金supported by the National Key Research and Development Program of China(2022YFF0711500)National Natural Science Foundation of China(NSFC,grant Nos.12373110,12273077,12103070,and 12333004)+3 种基金the Strategic Priority Research Program of the Chinese Academy of Sciences(grant Nos.XDA15310300,XDB0550200,XDB0550100,and XDB0550000)supported by China National Astronomical Data Center(NADC)Chinese Virtual Observatory(China-VO)supported by Astronomical Big Data Joint Research Center,cofounded by National Astronomical Observatories,Chinese Academy of Sciences and Alibaba Cloud。
文摘X-ray observations play a crucial role in time-domain astronomy.The Einstein Probe(EP),a recently launched X-ray astronomical satellite,emerges as a forefront player in the field of time-domain astronomy and high-energy astrophysics.With a focus on systematic surveys in the soft X-ray band,EP aims to discover high-energy transients and monitor variable sources in the universe.To achieve these objectives,a quick and reliable classification of observed sources is essential.In this study,we developed a machine learning classifier for autonomous source classification using data from the EP-WXT Pathfinder—Lobster Eye Imager for Astronomy(LEIA)and EP-WXT simulations.The proposed Random Forest classifier,built on selected features derived from light curves,energy spectra,and location information,achieves an accuracy of approximately 95%on EP simulation data and 98%on LEIA observational data.The classifier is integrated into the LEIA data processing pipeline,serving as a tool for manual validation and rapid classification during observations.This paper presents an efficient method for the classification of X-ray sources based on single observations,along with implications of most effective features for the task.This work facilitates rapid source classification for the EP mission and also provides valuable insights into feature selection and classification techniques for enhancing the efficiency and accuracy of X-ray source classification that can be adapted to other X-ray telescope data.
文摘The evaluation of the electricity market is crucial for fostering market construction and development.An accurate assessment of the electricity market reveals developmental trends,identifies operational issues,and contributes to stable and healthy market growth.This study investigated the characteristics of electricity markets in different provinces and synthesized a comprehensive set of evaluation indicators to assess market effectiveness.The evaluation framework,comprising nine indicators organized into two tiers,was constructed based on three aspects:market design,market efficiency,and developmental coordination.Furthermore,a novel fuzzy multi-criteria decision-making evaluation model for electricity market performance was developed based on the Fuzzy-BWM and fuzzy COPRAS methodologies.This model aimed to ensure both accuracy and comprehensiveness in market operation assessment.Subsequently,empirical analyses were conducted on four typical provincial-level electricity markets in China.The results indicate that Guangdong’s electricity market performed best because of its effective balance of stakeholder interests and adherence to contractual integrity principles.Zhejiang and Shandong ranked second and third,respectively,whereas Sichuan exhibited the poorest market performance.Sichuan’s electricity market must be improved in terms of market design,such that market players can obtain a fairly competitive environment.The sensitivity analysis of the constructed indicators verified the effectiveness of the evaluation model proposed in this study.Finally,policy recommendations were proposed to facilitate the sustainable development of China’s electricity markets with the objective of transforming them into efficient and secure markets adaptable to the evolution of novel power systems.
文摘3,3′,5-Triiodo-L-thyronine(T3)is a key endocrine hormone in the human body that plays crucial roles in growth,development,metabolism,and immune function.Macrophages,the key regulatory cells within the immune system,exhibit marked“heterogeneity”and“plasticity”,with their phenotype and function subject to modulation by local environmental signals.The interplay between the endocrine and immune systems is well documented.Numerous studies have shown that T3 significantly target macrophages,highlighting them as key cellular components in this interaction.Through the regulation of macrophage function and phenotype,T3 influences immune function and tissue repair in the body.This review comprehensively summarizes the regulatory actions and mechanisms of T3 on macrophages,offering valuable insights into further research of the immunoregulatory effects of T3.
基金Supported by 1·3·5 Project for Disciplines of Excellence,West China Hospital,Sichuan University,No.ZYJC21004.
文摘BACKGROUND Cyclic vomiting syndrome(CVS)is a chronic functional gastrointestinal disorder involving the gut–brain interaction that is characterized by recurring episodes of nausea,vomiting,abdominal pain,and interspersed complete normal periods.Superior mesenteric artery(SMA)syndrome(SMAS)is a vascular condition in which the horizontal portion of the duodenum is compressed due to a reduced angle between the aorta and the SMA.This condition presents with symptoms similar to CVS,posing challenges in distinguishing between the two and often resulting in misdiagnosis or inappropriate treatment.CASE SUMMARY A 20-year-old female patient presented with recurrent episodes of vomiting and experienced a persistent fear of vomiting for the past 2 years.She adopted conscious dietary restrictions,which led to severe malnutrition.Initially,she was diagnosed with SMAS,as revealed by computed tomography angiography.Despite efforts to increase the angle between the aorta and the SMA through weight gain,her vomiting did not improve.Finally,she was diagnosed with comorbidities including CVS,SMAS and anxiety disorder.She underwent comprehensive interventions,including enteral and parenteral nutritional supplementation,administration of antiemetic and anti-anxiety agents,and participation in mindfulness-based cognitive therapy.The patient eventually experienced a notable improvement in both body weight and clinical symptoms.CONCLUSION We present a rare case of CVS in an adult complicated with SMAS and propose additional treatment with nutritional support,pharmacological intervention,and psychotherapy.
文摘In the article‘MicroRNA-329-3p inhibits the Wnt/β-catenin pathway and proliferation of osteosarcoma cells by targeting transcription factor 7-like 1’(Oncology Research,2024,Vol.32,No.3,pp.463−476.doi:10.32604/or.2023.044085),there was an error in the compilation of Fig.8D.We have revised Fig.8D to correct this error.A corrected version of Fig.8 is provided.This correction does not change any results or conclusions of the article.We apologize for any inconvenience caused.
基金supported by a grant from Health Bureauof Jiangxi Province
文摘BACKGROUND： This study aimed to explore the effects of TNF-α on the expression of IP3R1 mRNA and protein in human mesangial cells （HMCs）, and to elucidate the mechanism of TNF-α relating to IP3R1 expression in the occurrence of hepatorenal syndrome （HRS）.METHODS： HMCs were stimulated by tumor （TNF-α） with 100 ng/mL for different hours （2, 4, 8, and 24 hours）. The expression changes of IP3R1 mRNA and protein were detected by quantitative real-time polymerase chain reaction and immunoblotting. Several inhibitors including D609, U73122, PP1, safingol, rottlerin and non-radioactive protein kinase C （PKC） were used to examine the mechanism of signal transduction of TNF-α-regulated IP3R1 in HMCs.RESULTS： The levels of IP3R1 mRNA at 2 hours after TNF-α exposure were significantly enhanced and peaked at 8 hours in HMCs （P〈0.01）, then descended at 24 hours （P〈0.01）. The levels of IP3R1 protein at 4 hours after TNF-α exposure were obviously increased and peaked at 24 hours after TNF-α exposure （P〈0.01）. Compared to the control group, safingol （PKCa inhibitor） and D609 （phosphatidylcholine-specific phospholipase C inhibitor） significantly blocked the TNF-α- induced expression of IP3R1 mRNA （3.30±0.81 vs. 1.95±0.13, P〈0.05; 2.10±0.49, P〈0.01） and IP3R1 protein （3.09±0.13 vs. 1.86±0.39, P〈0.01; 1.98±0.02, P〈0.01）. TNF-α promoted PKCa activation with maximal PKCa phosphorylation that occurred 8 hours after stimulation measured by non-radioactive PKC assay, and the effect was markedly attenuated by pretreatment with D609 or safingol.CONCLUSION： TNF-α increased the expression of IP3R1 and this was mediated, at least in part, through the PC-PLC/PKCa signaling pathways in HMCs.
基金Supported by the National Natural Science Foundation of China,No.81101806Natural Science Foundation of Shanghai,No.17ZR1406500
文摘Solitary rectal ulcer syndrome(SRUS) is a rare benign condition, which can mimic many other diseases because of their similarities in clinical, endoscopic and histological features. Sessile serrated adenoma/polyp(SSA/p) is a premalignant lesion in the colon and rectum. The misdiagnosis of SSA/p in SRUS patients has been noted, but the case of SRUS arising secondarily to SSA/p has been rarely reported. We herein report the case of a 59-year-old man who presented with an ulcerative nodular lesion in the rectum, accompanied by the symptoms of blood and mucus in the feces, diarrhea and constipation. Magnetic resonance imagining revealed thickening of the rectal mucosa-submucosa. Histologically, the lesion was characterized by the hyperplastic lamina propria and diffusely serrated crypts. Further immunohistochemical staining showed the loss of HES1 and MLH1 expression in the epithelial cells in the serrated area. The patient with SRUS had histological changes of SSA/p, suggesting a potential of tumor transformation in certain cases. SRUS uncommonly accompanied by serrated lesions should at least be considered by pathologists and clinicians.
基金supporting data analysis related to the patient experience survey。
文摘Background:In recent years,day surgery has gained widespread attention in the medical industry.This thesis focuses on three tertiary general hospitals in different regions in China where day surgery has been carried out earlier,is more developed,has a more comprehensive range of day surgery procedures,and has an annual volume of 10,000 or more day surgery cases.The study will provide hands-on experience for other medical institutions to carry out day surgery.Methods:67,337 valid items of data were retrieved from the Hospital Information Systems of the three hospitals.In this thesis,descriptive analysis was carried out on basic information about day surgery patients,including composition of patients’major diseases,hospitalization costs.Non-parametric tests were used to compare the data in each group,the median and interquartile range were used to show the trend in the concentration of the relevant indicators.The difference was considered statistically significant at P<0.05.Results:The largest proportion of day surgery patients were between the ages of 51 and 70.About 57.30%of day surgery patients paid for their medical care with the basic medical insurance of urban employees.Senile cataract,benign breast tumor,and endometrial polyp were the top three major diagnoses of day surgery patients.The average inpatient expenditure for day surgery patients was 8982.10 yuan,with consumables cost representing the highest proportion at approximately 46.01%of the total hospitalization cost,followed by treatment cost at around 31.01%of the total cost.Conclusions:Day surgery is more suitable for middle-aged patients and may not be the best choice for children and elderly patients over 80 years old.Most day surgery cases are elective surgeries for non-emergency situations.The distribution of day surgery patients is primarily concentrated in ophthalmic,breast,and reproductive system diseases,although each hospital has its own unique characteristics.The development of day surgery presents both economic and social benefits.
文摘Seasonally frozen soil in alpine and subalpine zones in the mountains of Qinghai-Tibetan Plateau is particularly sensitive to global climate change. Therefore, a better understanding of the thermal properties of frozen soil is crucial for predicting the responses of frozen soils to soil warming. In this study, thermal properties of frozen soil with different moisture contents under subzero temperature (0°C - 20°C) in an alpine forest in western Sichuan were analyzed by KD<sub>2</sub> Pro in its cooling and heating processes, respectively. Our results reveal that the soil apparent volumetric specific heat capacity (C<sub>v</sub>) and apparent thermal conductivity (K) under the same water content show similar response patterns to changing temperature lower than -2°C in both heating and cooling processes. Moreover, ice content of frozen soils can be well predicted by Logistic model in cooling and heating processes. The C<sub>v</sub> and K tend to increase along with increasing soil moisture contents. Remarkably, asymptotic characters of the value of C<sub>v</sub> and K are at the vicinity of the initial temperature of phase transitions, indicating that both C<sub>v</sub> and K are particularly sensitive to changing soil temperature at the range of -2°C to 0°C. Therefore, the widely distributed frozen soil layers with temperature above -2°C in alpine and subalpine zones over Qinghai-Tibetan Plateau are susceptible to the observed climate warming during cold season.
基金This work was supported by key projects of National Natural Science Foundation of China(No.81830110)Heilongjiang Province key research and development plan(No.2022ZX02C04).
