Decorative fishery is an important part of recreational fisheries,Hainan has the potential to develop decorative fishery.In this paper,on the basis of the deep-going survey on the current situation of decorative fishe...Decorative fishery is an important part of recreational fisheries,Hainan has the potential to develop decorative fishery.In this paper,on the basis of the deep-going survey on the current situation of decorative fishery development in Hainan,the main questions of decorative fish cultivation and market were discussed.At the same time,many suggestions were brought up,such as exploiting the biological resources of Hainan Island,strengthening scientific research efforts,introducing new varieties of ornamental species,strengthening industrial guidance and market management,etc.,which will benefit the rational utilization of tropical biological resources and increase the whole competitive advantage of decorative fishery in Hainan Province.展开更多
The integrity of retinal ganglion cells is tightly associated with diabetic macular degeneration that leads to damage and death of retinal ganglion cells,affecting vision.The major clinical treatments for diabetic mac...The integrity of retinal ganglion cells is tightly associated with diabetic macular degeneration that leads to damage and death of retinal ganglion cells,affecting vision.The major clinical treatments for diabetic macular edema are anti-vascular endothelial growth factor drugs and laser photocoagulation.However,although the macular thickness can be normalized with each of these two therapies used alone,the vision does not improve in many patients.This might result from the incomplete recovery of retinal ganglion cell injury.Therefore,a prospective,non-randomized,controlled clinical trial was designed to investigate the effect of anti-vascular endothelial growth factor drugs combined with laser photocoagulation on the integrity of retinal ganglion cells in patients with diabetic macular edema and its relationship with vision recovery.In this trial,150 patients with diabetic macular edema will be equally divided into three groups according to therapeutic methods,followed by treatment with anti-vascular endothelial growth factor drugs,laser photocoagulation therapy,and their combination.All patients will be followed up for 12 months.The primary outcome measure is retinal ganglion cell-inner plexiform layer thickness at 12 months after treatment.The secondary outcome measures include retinal ganglion cell-inner plexiform layer thickness before and 1,3,6,and 9 months after treatment,retinal nerve fiber layer thickness,best-corrected visual acuity,macular area thickness,and choroidal thickness before and 1,3,6,9,and 12 months after treatment.Safety measure is the incidence of adverse events at 1,3,6,9,and 12 months after treatment.The study protocol hopes to validate the better efficacy and safety of the combined treatment in patients with diabetic macula compared with the other two monotherapies alone during the 12-month follow-up period.The trial is designed to focus on clarifying the time-effect relationship between imaging measures related to the integrity of retinal ganglion cells and best-corrected visual acuity.The trial protocol was approved by the Medical Ethics Committee of the Affiliated Hospital of Beihua University with approval No.(2023)(26)on April 25,2023,and was registered with the Chinese Clinical Trial Registry(registration number:ChiCTR2300072478,June 14,2023,protocol version:2.0).展开更多
Proppant transport within fractures is one of the most critical tasks in oil,gas and geothermal reservoir stimulation,as it largely determines the ultimate performance of the operating well.Proppant transport in rough...Proppant transport within fractures is one of the most critical tasks in oil,gas and geothermal reservoir stimulation,as it largely determines the ultimate performance of the operating well.Proppant transport in rough fracture networks is still a relatively new area of research and the associated transport mechanisms are still unclear.In this study,representative parameters of rough fracture surfaces formed by supercritical CO_(2) fracturing were used to generate a rough fracture network model based on a spectral synthesis method.Computational fluid dynamics(CFD)coupled with the discrete element method(DEM)was used to study proppant transport in this rough fracture network.To reveal the turning transport mechanism of proppants into branching fractures at the intersections of rough fracture networks,a comparison was made with the behavior within smooth fracture networks,and the effect of key pumping parameters on the proppant placement in a secondary fracture was analyzed.The results show that the transport behavior of proppant in rough fracture networks is very different from that of the one in the smooth fracture networks.The turning transport mechanisms of proppant into secondary fractures in rough fracture networks are gravity-driven sliding,high velocity fluid suspension,and fracture structure induction.Under the same injection conditions,supercritical CO_(2)with high flow Reynolds number still has a weaker ability to transport proppant into secondary fractures than water.Thickening of the supercritical CO_(2)needs to be increased beyond a certain value to have a significant effect on proppant carrying,and under the temperature and pressure conditions of this paper,it needs to be increased more than 20 times(about 0.94 m Pa s).Increasing the injection velocity and decreasing the proppant concentration facilitates the entry of proppant into the branching fractures,which in turn results in a larger stimulated reservoir volume.The results help to understand the proppant transport and placement process in rough fracture networks formed by reservoir stimulation,and provide a theoretical reference for the optimization of proppant pumping parameters in hydraulic fracturing.展开更多
Diagnosing multi-stage diseases typically requires doctors to consider multiple data sources,including clinical symptoms,physical signs,biochemical test results,imaging findings,pathological examination data,and even ...Diagnosing multi-stage diseases typically requires doctors to consider multiple data sources,including clinical symptoms,physical signs,biochemical test results,imaging findings,pathological examination data,and even genetic data.When applying machine learning modeling to predict and diagnose multi-stage diseases,several challenges need to be addressed.Firstly,the model needs to handle multimodal data,as the data used by doctors for diagnosis includes image data,natural language data,and structured data.Secondly,privacy of patients’data needs to be protected,as these data contain the most sensitive and private information.Lastly,considering the practicality of the model,the computational requirements should not be too high.To address these challenges,this paper proposes a privacy-preserving federated deep learning diagnostic method for multi-stage diseases.This method improves the forward and backward propagation processes of deep neural network modeling algorithms and introduces a homomorphic encryption step to design a federated modeling algorithm without the need for an arbiter.It also utilizes dedicated integrated circuits to implement the hardware Paillier algorithm,providing accelerated support for homomorphic encryption in modeling.Finally,this paper designs and conducts experiments to evaluate the proposed solution.The experimental results show that in privacy-preserving federated deep learning diagnostic modeling,the method in this paper achieves the same modeling performance as ordinary modeling without privacy protection,and has higher modeling speed compared to similar algorithms.展开更多
The plant hormone auxin plays a critical role in regulating plant growth and development. Recent advances have been made in the understanding of auxin response pathways, primarily by the characterization of auxin resp...The plant hormone auxin plays a critical role in regulating plant growth and development. Recent advances have been made in the understanding of auxin response pathways, primarily by the characterization of auxin response mutants in Arabidopsis. In addition, microRNAs (miRNAs) have been shown to be critical regulators of genes important for normal plant development and physiology. However, little is known about possible interactions between miRNAs and hormonal signaling during normal development. Here we show that an Arabidopsis microRNA, miR167, which has a complementary sequence to a portion of the A UXINRESPONSE FACTOR6 (ARF6) and ARF8 mRNAs, can cause transcript degradation for ARF8, but not for ARF6. We report phenotypic characterizations of 35S::MIR167b transgenic lines, and show that severe 35S::MIR167b transgenic lines had phenotypes similar to those of an arf6 arf8 double mutant. The transgenic phenotypes suggest that miR167 may repress ARF6 at the level of translation. We demonstrate that the transgenic plants are defective in all four whods of floral organs. In the transgenic flowers, filaments were abnormally short, anthers could not properly release pollen, and pollen grains did not germinate. Our results provide an important link between the miRNA-mediated regulatory pathway of gene expression and the auxin signaling network promoting plant reproductive development.展开更多
Molecular and genetic characterizations of mutants have led to a better understanding of many developmental processes in the model system Arabidopsis thaliana. However, the leaf development that is specific to plants ...Molecular and genetic characterizations of mutants have led to a better understanding of many developmental processes in the model system Arabidopsis thaliana. However, the leaf development that is specific to plants has been little studied. With the aim of contributing to the genetic dissection of leaf development, we have performed a large-scare screening for mutants with abnormal leaves. Among a great number of leaf mutants we have generated by T-DNA and transposon tagging and ethylmethae sulfonate (EMS) mutagenesis, four independent mutant lines have been identified and studied genetically. Phenotypes of these mutant lines represent the defects of four novel nuclear genes designated LL1 (LOTUS LEAF 1), LL2 (LOTUS LEAF 2), URO (UPRIGHT ROSETTE), and EIL (ENVIRONT CONDITION INDUCED LESION). The phenotypic analysis indicates that these genes play important roles during leaf development. FOr the further genetic analysis of these genes and the map-based cloning of LL1 and LL2, we have mapped these genes to chromosome regions with an efficient and rapid mapping method.展开更多
Objective:To investigate the mechanism of expression and significance of vascular endothelial growth factor(VEGF) and p53 in degenerate intervertebral disc tissue.Methods:Pathological sections collected from 156 patie...Objective:To investigate the mechanism of expression and significance of vascular endothelial growth factor(VEGF) and p53 in degenerate intervertebral disc tissue.Methods:Pathological sections collected from 156 patienls with lumbar disc herniation after surgery were tested by immunohistochemistry method,for evaluation of the expression of VEGF and p53 in degenerate intervertebral disc tissue.Results:98 cases(62.8%) with vascular infiltration phenomenon are found,and positive rates of VEGF and p53 in degenerate intervertebral disc tissue are 73.42% (116/156) and 58.97%(92/156);co-expression rate is 53.2%(83/l56);the expression rates of VEFG and p53 are significantly higher in the tissue with blood vessel infiltration than in the tissue without infiltration;there is a close relationship of VEGF with p53.Conclusions:VEGF and p53 gene synergetic express in degenerate intervertebral disc tissue,working together in neovascularization and infiltration,and accelerating intervertebral disc tissue degeneration.展开更多
Tight sands are abundant in nanopores leading to a high capillary pressure and normally a low fluid injectivity.As such,spontaneous imbibition might be an effective mechanism for improving oil recovery from tight sand...Tight sands are abundant in nanopores leading to a high capillary pressure and normally a low fluid injectivity.As such,spontaneous imbibition might be an effective mechanism for improving oil recovery from tight sands after fracturing.The chemical agents added to the injected water can alter the interfacial properties,which could help further enhance the oil recovery by spontaneous imbibition.This study explores the possibility of using novel chemicals to enhance oil recovery from tight sands via spontaneous imbibition.We experimentally examine the effects of more than ten different chemical agents on spontaneous imbibition,including a cationic surfactant(C12 TAB),two anionic surfactants(0242 and 0342),an ionic liquid(BMMIM BF4),a high pH solution(NaBO2),and a series of house-made deep eutectic solvents(DES3-7,9,11,and 14).The interfacial tensions(IFT)between oil phase and some chemical solutions are also determined.Experimental results indicate that both the ionic liquid and cationic surfactant used in this study are detrimental to spontaneous imbibition and decrease the oil recovery from tight sands,even though cationic surfactant significantly decreases the oil-water IFT while ionic liquid does not.The high pH NaBO2 solution does not demonstrate significant effect on oil recovery improvement and IFT reduction.The anionic surfactants(O242 and O342)are effective in enhancing oil recovery from tight sands through oil-water IFT reduction and emulsification effects.The DESs drive the rock surface to be more water-wet,and a specific formulation(DES9)leads to much improvement on oil recovery under counter-current imbibition condition.This preliminary study would provide some knowledge about how to optimize the selection of chemicals for improving oil recovery from tight reservoirs.展开更多
Objective: Germline alterations in the breast cancer susceptibility genes type 1 and 2, BRCA1 and BRCA2, predispose individuals to hereditary cancers, including breast, ovarian, prostate, pancreatic, and stomach cance...Objective: Germline alterations in the breast cancer susceptibility genes type 1 and 2, BRCA1 and BRCA2, predispose individuals to hereditary cancers, including breast, ovarian, prostate, pancreatic, and stomach cancers.Accumulating evidence suggests inherited genetic susceptibility to lung cancer.The present study aimed to survey the prevalence of pathogenic germline BRCA mutations(gBRCAm) and explore the potential association between gBRCAm and disease onset in Chinese advanced non-small cell lung cancer(NSCLC) patients.Methods: A total of 6,220 NSCLC patients were screened using capture-based ultra-deep targeted sequencing to identify patients harboring germline BRCA1/2 mutations.Results: Out of the 6,220 patients screened, 1.03%(64/6,220) of the patients harbored the pathogenic gB RCAm, with BRCA2 mutations being the most predominant mutations(49/64, 76.5%).Patients who developed NSCLC before 50 years of age were more likely to carry gBRCAm(P = 0.036).Among the patients harboring classic lung cancer driver mutations, those with concurrent gBRCAm were significantly younger than those harboring the wild-type gBRCA(P = 0.029).By contrast, the age of patients with or without concurrent gBRCAm was comparable to those of patients without the driver mutations(P = 0.972).In addition, we identified EGFR-mutant patients with concurrent gBRCAm who showed comparable progression-free survival but significantly longer overall survival(P = 0.002) compared to EGFR-mutant patients with wild-type germline BRCA.Conclusions: Overall, our study is the largest survey of the prevalence of pathogenic gBRCAm in advanced Chinese NSCLC patients.Results suggested a lack of association between germline BRCA status and treatment outcome of EGFR-TKI.In addition,results showed a positive correlation between pathogenic gB RCAm and an early onset of NSCLC.展开更多
Underwater vehicles are being emphasized as highly integrated and intelligent devices for a significant number of oceanic operations. However, their precise operation is usually hindered by disturbances from a tether ...Underwater vehicles are being emphasized as highly integrated and intelligent devices for a significant number of oceanic operations. However, their precise operation is usually hindered by disturbances from a tether or manipulator because their propellers are unable to realize a stable suspension. A dynamic multi-body model-based adaptive controller was designed to allow the controller of the vehicle to observe and compensate for disturbances from a tether or manipulator. Disturbances, including those from a tether or manipulator, are deduced for the observation of the controller. An analysis of a tether disturbance covers the conditions of the surface, the underwater area, and the vehicle end point. Interactions between the vehicle and manipulator are mainly composed of coupling forces and restoring moments.To verify the robustness of the controller, path-following experiments on a streamlined autonomous underwater vehicle experiencing various disturbances were conducted in Song Hua Lake in China. Furthermore,path-following experiments for a tethered open frame remote operated vehicle were verified for accurate cruising with a controller and an observer, and vehicle and manipulator coordinate motion control during the simulation and experiments verified the effectiveness of the controller and observer for underwater operation. This study provides instructions for the control of an underwater vehicle experiencing disturbances from a tether or manipulator.展开更多
Objective:To explore the genetic changes in the progression of castration-resistant prostate cancer(CRPC)and neuroendocrine prostate cancer(NEPC)and the reason why these cancers resist existing therapies.Methods:We em...Objective:To explore the genetic changes in the progression of castration-resistant prostate cancer(CRPC)and neuroendocrine prostate cancer(NEPC)and the reason why these cancers resist existing therapies.Methods:We employed our CRPC cell line microarray and other CRPC or NEPC datasets to screen the target gene NEIL3.Lentiviral transfection and RNA interference were used to construct overexpression and knockdown cell lines.Cell and animal models of radiotherapy were established by using a medical electron linear accelerator.Flow cytometry was used to detect apoptosis or cell cycle progression.Western blot and qPCR were used to detect changes in the protein and RNA levels.Results:TCGA and clinical patient datasets indicated that NEIL3 was downregulated in CRPC and NEPC cell lines,and NEIL3 was correlated with a high Gleason score but a good prognosis.Further functional studies demonstrated that NEIL3 had no effect on the proliferation and migration of PCa cells.However,cell and animal radiotherapy models revealed that NEIL3 could facilitate the radiotherapy sensitivity of PCa cells,while loss of NEIL3 activated radiotherapy resistance.Mechanistically,we found that NEIL3 negatively regulated the expression of ATR,and higher NEIL3 expression repressed the ATR/CHK1 pathway,thus regulating the cell cycle.Conclusions:We demonstrated that NEIL3 may serve as a diagnostic or therapeutic target for therapy-resistant patients.展开更多
The water pollution situation in Balihe Lake, the biggest tributary of Shaying River Basin in Anhui Province, China, has brought a huge pressure on the improvement of water quality in Huai River. On October 16th, 2017...The water pollution situation in Balihe Lake, the biggest tributary of Shaying River Basin in Anhui Province, China, has brought a huge pressure on the improvement of water quality in Huai River. On October 16th, 2017, 11 major pollution indexes were observed at 15 sampling points in Balihe Lake. Based on the data experimentally measured, the water quality in Balihe Lake was analyzed utilizing the Principal Component Analysis (PCA) of SPSS. The result suggested that the major components were oxygenated pollutants, water eutrophication pollutants and ammonia nitrogen, in which oxygenated pollutants played a dominant role. In addition, the upper part of Balihe Lake suffered serious situation and needed a focus on oxygenated pollutants.展开更多
The number of mobile application services is showing an explosive growth trend,which makes it difficult for users to determine which ones are of interest.Especially,the new mobile application services are emerge conti...The number of mobile application services is showing an explosive growth trend,which makes it difficult for users to determine which ones are of interest.Especially,the new mobile application services are emerge continuously,most of them have not be rated when they need to be recommended to users.This is the typical problem of cold start in the field of collaborative filtering recommendation.This problem may makes it difficult for users to locate and acquire the services that they actually want,and the accuracy and novelty of service recommendations are also difficult to satisfy users.To solve this problem,a hybrid recommendation method for mobile application services based on content feature extraction is proposed in this paper.First,the proposed method in this paper extracts service content features through Natural Language Processing technologies such as word segmentation,part-of-speech tagging,and dependency parsing.It improves the accuracy of describing service attributes and the rationality of the method of calculating service similarity.Then,a language representation model called Bidirectional Encoder Representation from Transformers(BERT)is used to vectorize the content feature text,and an improved weighted word mover’s distance algorithm based on Term Frequency-Inverse Document Frequency(TFIDF-WMD)is used to calculate the similarity of mobile application services.Finally,the recommendation process is completed by combining the item-based collaborative filtering recommendation algorithm.The experimental results show that by using the proposed hybrid recommendation method presented in this paper,the cold start problem is alleviated to a certain extent,and the accuracy of the recommendation result has been significantly improved.展开更多
In the published article1,the affiliation for the first author,Xingshcng Hu,is"Department of Medical Oncology,Cancer Hospital,Chinese Academy of Medical Sciences",we would like to update it to"Departmen...In the published article1,the affiliation for the first author,Xingshcng Hu,is"Department of Medical Oncology,Cancer Hospital,Chinese Academy of Medical Sciences",we would like to update it to"Department of Medical Oncology,National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing,100021,China".We apologize for the errors and for any confusion it may have caused.展开更多
基金Supported by the Natural Science Foundation of Hainan Province(311056)Key Scientific and Technological Project of Sanya City,Hainan Province(2010YF09)Key Laboratory Project of Sanya City,Hainan Province(L1222)
文摘Decorative fishery is an important part of recreational fisheries,Hainan has the potential to develop decorative fishery.In this paper,on the basis of the deep-going survey on the current situation of decorative fishery development in Hainan,the main questions of decorative fish cultivation and market were discussed.At the same time,many suggestions were brought up,such as exploiting the biological resources of Hainan Island,strengthening scientific research efforts,introducing new varieties of ornamental species,strengthening industrial guidance and market management,etc.,which will benefit the rational utilization of tropical biological resources and increase the whole competitive advantage of decorative fishery in Hainan Province.
基金supported by Science and Technology Research Project of Jilin Provincial Department of Education,No.JJKH20220072KJ(to XL)Science and Technology Development Program of Jilin Province,No.20200201495JC(to YL)。
文摘The integrity of retinal ganglion cells is tightly associated with diabetic macular degeneration that leads to damage and death of retinal ganglion cells,affecting vision.The major clinical treatments for diabetic macular edema are anti-vascular endothelial growth factor drugs and laser photocoagulation.However,although the macular thickness can be normalized with each of these two therapies used alone,the vision does not improve in many patients.This might result from the incomplete recovery of retinal ganglion cell injury.Therefore,a prospective,non-randomized,controlled clinical trial was designed to investigate the effect of anti-vascular endothelial growth factor drugs combined with laser photocoagulation on the integrity of retinal ganglion cells in patients with diabetic macular edema and its relationship with vision recovery.In this trial,150 patients with diabetic macular edema will be equally divided into three groups according to therapeutic methods,followed by treatment with anti-vascular endothelial growth factor drugs,laser photocoagulation therapy,and their combination.All patients will be followed up for 12 months.The primary outcome measure is retinal ganglion cell-inner plexiform layer thickness at 12 months after treatment.The secondary outcome measures include retinal ganglion cell-inner plexiform layer thickness before and 1,3,6,and 9 months after treatment,retinal nerve fiber layer thickness,best-corrected visual acuity,macular area thickness,and choroidal thickness before and 1,3,6,9,and 12 months after treatment.Safety measure is the incidence of adverse events at 1,3,6,9,and 12 months after treatment.The study protocol hopes to validate the better efficacy and safety of the combined treatment in patients with diabetic macula compared with the other two monotherapies alone during the 12-month follow-up period.The trial is designed to focus on clarifying the time-effect relationship between imaging measures related to the integrity of retinal ganglion cells and best-corrected visual acuity.The trial protocol was approved by the Medical Ethics Committee of the Affiliated Hospital of Beihua University with approval No.(2023)(26)on April 25,2023,and was registered with the Chinese Clinical Trial Registry(registration number:ChiCTR2300072478,June 14,2023,protocol version:2.0).
基金the support from the National Key Research and Development Program of China(Grant No.2022YFE0137200)the Natural Science Basic Research Program of Shaanxi Province,China(Program No.2024JC-YBQN-0381,2023JC-QN-0403)+2 种基金the Natural Science Basic Research Program of Shaanxi Province,China(Program No.2022JC-37)the Innovation Capability Support Program of Shaanxi(Program No.2023-CX-TD31)the Funded by Open Foundation of Shaanxi Key Laboratory of Carbon Dioxide Sequestration and Enhanced Oil Recovery,and the Youth Innovation Team of Shaanxi Universities。
文摘Proppant transport within fractures is one of the most critical tasks in oil,gas and geothermal reservoir stimulation,as it largely determines the ultimate performance of the operating well.Proppant transport in rough fracture networks is still a relatively new area of research and the associated transport mechanisms are still unclear.In this study,representative parameters of rough fracture surfaces formed by supercritical CO_(2) fracturing were used to generate a rough fracture network model based on a spectral synthesis method.Computational fluid dynamics(CFD)coupled with the discrete element method(DEM)was used to study proppant transport in this rough fracture network.To reveal the turning transport mechanism of proppants into branching fractures at the intersections of rough fracture networks,a comparison was made with the behavior within smooth fracture networks,and the effect of key pumping parameters on the proppant placement in a secondary fracture was analyzed.The results show that the transport behavior of proppant in rough fracture networks is very different from that of the one in the smooth fracture networks.The turning transport mechanisms of proppant into secondary fractures in rough fracture networks are gravity-driven sliding,high velocity fluid suspension,and fracture structure induction.Under the same injection conditions,supercritical CO_(2)with high flow Reynolds number still has a weaker ability to transport proppant into secondary fractures than water.Thickening of the supercritical CO_(2)needs to be increased beyond a certain value to have a significant effect on proppant carrying,and under the temperature and pressure conditions of this paper,it needs to be increased more than 20 times(about 0.94 m Pa s).Increasing the injection velocity and decreasing the proppant concentration facilitates the entry of proppant into the branching fractures,which in turn results in a larger stimulated reservoir volume.The results help to understand the proppant transport and placement process in rough fracture networks formed by reservoir stimulation,and provide a theoretical reference for the optimization of proppant pumping parameters in hydraulic fracturing.
基金funded by the National Natural Science Foundation,China(No.62172123)the Key Research and Development Program of Heilongjiang(Grant No.2022ZX01A36)+1 种基金the Special Projects for the Central Government to Guide the Development of Local Science and Technology,China(No.ZY20B11)the Harbin Manufacturing Technology Innovation Talent Project(No.CXRC20221104236).
文摘Diagnosing multi-stage diseases typically requires doctors to consider multiple data sources,including clinical symptoms,physical signs,biochemical test results,imaging findings,pathological examination data,and even genetic data.When applying machine learning modeling to predict and diagnose multi-stage diseases,several challenges need to be addressed.Firstly,the model needs to handle multimodal data,as the data used by doctors for diagnosis includes image data,natural language data,and structured data.Secondly,privacy of patients’data needs to be protected,as these data contain the most sensitive and private information.Lastly,considering the practicality of the model,the computational requirements should not be too high.To address these challenges,this paper proposes a privacy-preserving federated deep learning diagnostic method for multi-stage diseases.This method improves the forward and backward propagation processes of deep neural network modeling algorithms and introduces a homomorphic encryption step to design a federated modeling algorithm without the need for an arbiter.It also utilizes dedicated integrated circuits to implement the hardware Paillier algorithm,providing accelerated support for homomorphic encryption in modeling.Finally,this paper designs and conducts experiments to evaluate the proposed solution.The experimental results show that in privacy-preserving federated deep learning diagnostic modeling,the method in this paper achieves the same modeling performance as ordinary modeling without privacy protection,and has higher modeling speed compared to similar algorithms.
文摘The plant hormone auxin plays a critical role in regulating plant growth and development. Recent advances have been made in the understanding of auxin response pathways, primarily by the characterization of auxin response mutants in Arabidopsis. In addition, microRNAs (miRNAs) have been shown to be critical regulators of genes important for normal plant development and physiology. However, little is known about possible interactions between miRNAs and hormonal signaling during normal development. Here we show that an Arabidopsis microRNA, miR167, which has a complementary sequence to a portion of the A UXINRESPONSE FACTOR6 (ARF6) and ARF8 mRNAs, can cause transcript degradation for ARF8, but not for ARF6. We report phenotypic characterizations of 35S::MIR167b transgenic lines, and show that severe 35S::MIR167b transgenic lines had phenotypes similar to those of an arf6 arf8 double mutant. The transgenic phenotypes suggest that miR167 may repress ARF6 at the level of translation. We demonstrate that the transgenic plants are defective in all four whods of floral organs. In the transgenic flowers, filaments were abnormally short, anthers could not properly release pollen, and pollen grains did not germinate. Our results provide an important link between the miRNA-mediated regulatory pathway of gene expression and the auxin signaling network promoting plant reproductive development.
基金supported by a grant from the Chinese Academy of Sciences,KJ951-B1-604 and a National Distinguished Young Scholar Award to Hai HUANG.
文摘Molecular and genetic characterizations of mutants have led to a better understanding of many developmental processes in the model system Arabidopsis thaliana. However, the leaf development that is specific to plants has been little studied. With the aim of contributing to the genetic dissection of leaf development, we have performed a large-scare screening for mutants with abnormal leaves. Among a great number of leaf mutants we have generated by T-DNA and transposon tagging and ethylmethae sulfonate (EMS) mutagenesis, four independent mutant lines have been identified and studied genetically. Phenotypes of these mutant lines represent the defects of four novel nuclear genes designated LL1 (LOTUS LEAF 1), LL2 (LOTUS LEAF 2), URO (UPRIGHT ROSETTE), and EIL (ENVIRONT CONDITION INDUCED LESION). The phenotypic analysis indicates that these genes play important roles during leaf development. FOr the further genetic analysis of these genes and the map-based cloning of LL1 and LL2, we have mapped these genes to chromosome regions with an efficient and rapid mapping method.
文摘Objective:To investigate the mechanism of expression and significance of vascular endothelial growth factor(VEGF) and p53 in degenerate intervertebral disc tissue.Methods:Pathological sections collected from 156 patienls with lumbar disc herniation after surgery were tested by immunohistochemistry method,for evaluation of the expression of VEGF and p53 in degenerate intervertebral disc tissue.Results:98 cases(62.8%) with vascular infiltration phenomenon are found,and positive rates of VEGF and p53 in degenerate intervertebral disc tissue are 73.42% (116/156) and 58.97%(92/156);co-expression rate is 53.2%(83/l56);the expression rates of VEFG and p53 are significantly higher in the tissue with blood vessel infiltration than in the tissue without infiltration;there is a close relationship of VEGF with p53.Conclusions:VEGF and p53 gene synergetic express in degenerate intervertebral disc tissue,working together in neovascularization and infiltration,and accelerating intervertebral disc tissue degeneration.
基金support provided through a NSERC Discovery Grant(No:RES0011227)to T.Babadaglia NSERC Discovery Grant(No:NSERC RGPIN 05394)to H.Li+3 种基金financial supports provided by National Natural Science Foundation of China(No:51874240)National Science and Technology Major Project(No:2016ZX05047003-004)the Key Laboratory Fund of Education Department of Shaanxi Province(No:15JS086)Ph.D.Scholarship from the China Scholarship Council(CSC)(201806450029)for the financial support.
文摘Tight sands are abundant in nanopores leading to a high capillary pressure and normally a low fluid injectivity.As such,spontaneous imbibition might be an effective mechanism for improving oil recovery from tight sands after fracturing.The chemical agents added to the injected water can alter the interfacial properties,which could help further enhance the oil recovery by spontaneous imbibition.This study explores the possibility of using novel chemicals to enhance oil recovery from tight sands via spontaneous imbibition.We experimentally examine the effects of more than ten different chemical agents on spontaneous imbibition,including a cationic surfactant(C12 TAB),two anionic surfactants(0242 and 0342),an ionic liquid(BMMIM BF4),a high pH solution(NaBO2),and a series of house-made deep eutectic solvents(DES3-7,9,11,and 14).The interfacial tensions(IFT)between oil phase and some chemical solutions are also determined.Experimental results indicate that both the ionic liquid and cationic surfactant used in this study are detrimental to spontaneous imbibition and decrease the oil recovery from tight sands,even though cationic surfactant significantly decreases the oil-water IFT while ionic liquid does not.The high pH NaBO2 solution does not demonstrate significant effect on oil recovery improvement and IFT reduction.The anionic surfactants(O242 and O342)are effective in enhancing oil recovery from tight sands through oil-water IFT reduction and emulsification effects.The DESs drive the rock surface to be more water-wet,and a specific formulation(DES9)leads to much improvement on oil recovery under counter-current imbibition condition.This preliminary study would provide some knowledge about how to optimize the selection of chemicals for improving oil recovery from tight reservoirs.
基金supported by grant from the National Natural Science Foundation of China (Grant No.81502699)
文摘Objective: Germline alterations in the breast cancer susceptibility genes type 1 and 2, BRCA1 and BRCA2, predispose individuals to hereditary cancers, including breast, ovarian, prostate, pancreatic, and stomach cancers.Accumulating evidence suggests inherited genetic susceptibility to lung cancer.The present study aimed to survey the prevalence of pathogenic germline BRCA mutations(gBRCAm) and explore the potential association between gBRCAm and disease onset in Chinese advanced non-small cell lung cancer(NSCLC) patients.Methods: A total of 6,220 NSCLC patients were screened using capture-based ultra-deep targeted sequencing to identify patients harboring germline BRCA1/2 mutations.Results: Out of the 6,220 patients screened, 1.03%(64/6,220) of the patients harbored the pathogenic gB RCAm, with BRCA2 mutations being the most predominant mutations(49/64, 76.5%).Patients who developed NSCLC before 50 years of age were more likely to carry gBRCAm(P = 0.036).Among the patients harboring classic lung cancer driver mutations, those with concurrent gBRCAm were significantly younger than those harboring the wild-type gBRCA(P = 0.029).By contrast, the age of patients with or without concurrent gBRCAm was comparable to those of patients without the driver mutations(P = 0.972).In addition, we identified EGFR-mutant patients with concurrent gBRCAm who showed comparable progression-free survival but significantly longer overall survival(P = 0.002) compared to EGFR-mutant patients with wild-type germline BRCA.Conclusions: Overall, our study is the largest survey of the prevalence of pathogenic gBRCAm in advanced Chinese NSCLC patients.Results suggested a lack of association between germline BRCA status and treatment outcome of EGFR-TKI.In addition,results showed a positive correlation between pathogenic gB RCAm and an early onset of NSCLC.
基金Supported by National Natural Science Foundation of China(Grant Nos.5129050,51579053,61633009)Major National Science and Technology Project of China(Grant No.2015ZX01041101)Key Basic Research Project of "Shanghai Science and Technology Innovation Plan" of China (Grant No.15JC1403300)
文摘Underwater vehicles are being emphasized as highly integrated and intelligent devices for a significant number of oceanic operations. However, their precise operation is usually hindered by disturbances from a tether or manipulator because their propellers are unable to realize a stable suspension. A dynamic multi-body model-based adaptive controller was designed to allow the controller of the vehicle to observe and compensate for disturbances from a tether or manipulator. Disturbances, including those from a tether or manipulator, are deduced for the observation of the controller. An analysis of a tether disturbance covers the conditions of the surface, the underwater area, and the vehicle end point. Interactions between the vehicle and manipulator are mainly composed of coupling forces and restoring moments.To verify the robustness of the controller, path-following experiments on a streamlined autonomous underwater vehicle experiencing various disturbances were conducted in Song Hua Lake in China. Furthermore,path-following experiments for a tethered open frame remote operated vehicle were verified for accurate cruising with a controller and an observer, and vehicle and manipulator coordinate motion control during the simulation and experiments verified the effectiveness of the controller and observer for underwater operation. This study provides instructions for the control of an underwater vehicle experiencing disturbances from a tether or manipulator.
基金supported by the National Natural Science Foundation of China(Grant Nos.81672550 and 81974395)Guangdong Basic and Applied Basic Research Foundation(Grant No.2019A1515011437)+4 种基金Guangzhou Science and Technology Cooperation Program(Foreign Research and Development Cooperation)(Grant No.201807010087)Clinical Research 5010 Program of Sun Yat-sen University(Grant No.2019005)Sun Yat-Sen Clinical Research and Cultivation Project of Sun Yat Sen University(Grant No.201702)to Hai Huangsupported by the National Natural Science Foundation of China(Grant No.81702527)supported by the China Scholarship Council(Grant No.201906380075).
文摘Objective:To explore the genetic changes in the progression of castration-resistant prostate cancer(CRPC)and neuroendocrine prostate cancer(NEPC)and the reason why these cancers resist existing therapies.Methods:We employed our CRPC cell line microarray and other CRPC or NEPC datasets to screen the target gene NEIL3.Lentiviral transfection and RNA interference were used to construct overexpression and knockdown cell lines.Cell and animal models of radiotherapy were established by using a medical electron linear accelerator.Flow cytometry was used to detect apoptosis or cell cycle progression.Western blot and qPCR were used to detect changes in the protein and RNA levels.Results:TCGA and clinical patient datasets indicated that NEIL3 was downregulated in CRPC and NEPC cell lines,and NEIL3 was correlated with a high Gleason score but a good prognosis.Further functional studies demonstrated that NEIL3 had no effect on the proliferation and migration of PCa cells.However,cell and animal radiotherapy models revealed that NEIL3 could facilitate the radiotherapy sensitivity of PCa cells,while loss of NEIL3 activated radiotherapy resistance.Mechanistically,we found that NEIL3 negatively regulated the expression of ATR,and higher NEIL3 expression repressed the ATR/CHK1 pathway,thus regulating the cell cycle.Conclusions:We demonstrated that NEIL3 may serve as a diagnostic or therapeutic target for therapy-resistant patients.
文摘The water pollution situation in Balihe Lake, the biggest tributary of Shaying River Basin in Anhui Province, China, has brought a huge pressure on the improvement of water quality in Huai River. On October 16th, 2017, 11 major pollution indexes were observed at 15 sampling points in Balihe Lake. Based on the data experimentally measured, the water quality in Balihe Lake was analyzed utilizing the Principal Component Analysis (PCA) of SPSS. The result suggested that the major components were oxygenated pollutants, water eutrophication pollutants and ammonia nitrogen, in which oxygenated pollutants played a dominant role. In addition, the upper part of Balihe Lake suffered serious situation and needed a focus on oxygenated pollutants.
基金Project supported by the National Natural Science Foundation,China(No.62172123)the Postdoctoral Science Foundation of Heilongjiang Province,China(No.LBH-Z19067)+1 种基金the special projects for the central government to guide the development of local science and technology,China(No.ZY20B11)the Natural Science Foundation of Heilongjiang Province,China(No.QC2018081).
文摘The number of mobile application services is showing an explosive growth trend,which makes it difficult for users to determine which ones are of interest.Especially,the new mobile application services are emerge continuously,most of them have not be rated when they need to be recommended to users.This is the typical problem of cold start in the field of collaborative filtering recommendation.This problem may makes it difficult for users to locate and acquire the services that they actually want,and the accuracy and novelty of service recommendations are also difficult to satisfy users.To solve this problem,a hybrid recommendation method for mobile application services based on content feature extraction is proposed in this paper.First,the proposed method in this paper extracts service content features through Natural Language Processing technologies such as word segmentation,part-of-speech tagging,and dependency parsing.It improves the accuracy of describing service attributes and the rationality of the method of calculating service similarity.Then,a language representation model called Bidirectional Encoder Representation from Transformers(BERT)is used to vectorize the content feature text,and an improved weighted word mover’s distance algorithm based on Term Frequency-Inverse Document Frequency(TFIDF-WMD)is used to calculate the similarity of mobile application services.Finally,the recommendation process is completed by combining the item-based collaborative filtering recommendation algorithm.The experimental results show that by using the proposed hybrid recommendation method presented in this paper,the cold start problem is alleviated to a certain extent,and the accuracy of the recommendation result has been significantly improved.
文摘In the published article1,the affiliation for the first author,Xingshcng Hu,is"Department of Medical Oncology,Cancer Hospital,Chinese Academy of Medical Sciences",we would like to update it to"Department of Medical Oncology,National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing,100021,China".We apologize for the errors and for any confusion it may have caused.