LncRNA MEG3 acts a biomarker and regulates cell functions by targeting ADAR1 in colorectal cancer

BACKGROUND Colorectal cancer (CRC) is the third most prevalent malignancy and has the fourth highest global cancer mortality rate. Early diagnosis and prompt medical attention can improve quality of life and the prognosis of CRC patients. Accumulating evidence reveals that long non-coding RNAs (lncRNAs) function as oncogenes or anti-oncogenes, as well as biomarkers in various cancers. AIM To investigate the levels and molecular mechanism of the lncRNA maternally expressed gene 3 (MEG3) in CRC. METHODS The levels of lncRNA MEG3 in CRC tissue, serum and cell line samples were explored via qRT-PCR. The relationship between MEG3 levels and clinicopathological features in CRC was investigated. The diagnostic and prognostic values of serum MEG3 levels were analyzed with ROC curves and KaplanMeier survival curves, respectively. RESULTS Significant decreased levels of MEG3 existed in CRC tissue, cell lines and serum. CRC patients with down-regulated serum MEG3 levels had larger tumor sizes, and advanced clinical stages. The sensitivity and specificity of serum MEG3 levels in CRC detection was 0.667 and 0.875, respectively. Tumor size, T stages, and serum MEG3 levels are indie factors that produce an effect on CRC patients' prognosis. KaplanMeier survival curves suggested that CRC patients with high levels of MEG3 had a remarkably better overall survival rate. CONCLUSION LncRNA MEG3 is down-regulated in CRC, and regulates cell functions by targeting adenosine deaminase’s effect on RNA 1 in CRC.

Effect of Yuchang suppository on PPAR γ/NF-KB signaling pathway in rats with ulcerative colitis

Objective: To investigate the therapeutic effect of Yuchang suppository on experimental ulcerative colitis rats and the effect of PPARγ/NF-KB signaling pathway. Methods: The UC rat model was replicated by 2.4.6-trinitrobenzenesulfonic acid (TNBS)/ethanol method and randomly divided into normal control group, model group, high-dose group of Yuchang suppository, middle dose group , Low-dose group and sulfasalazine group, 8 rats in each group. After 14 days of treatment, the general condition of the rats was observed and the disease activity index was scored. The changes of serum TNF-α, IL-1β and IL-6 levels were detected by ELISA. The colonic mucosal injury index (CMDI) score and colon were observed by colon specimens. Histopathological changes;RT-PCR and Western blot were used to detect the expression of PPARγ and NF-KB genes and proteins in colon tissue. Results: Compared with the normal control group, the DAI and CMDI scores in the model group and the levels of serum TNF-α, IL-1β, IL-6, NF-κB p65 mRNA and protein in colon tissue were significantly increased, PPAR. - γ mRNA and protein expression levels were significantly reduced. The levels of IL-1β, IL-6, TNF-α and the expression of NF-κB p65 mRNA and protein in colon tissue were lower than those in the model group, and the expression levels of PPARγ mRNA and protein were high. In the model group. In the improvement of NF-κBp65 and PPAR-γ gene and protein expression in rat colonic mucosa, the high-dose group and the middle-dose group were significantly better than the low-dose group. Conclusion:Yuchang suppository can significantly improve the symptoms and histopathology of UC model rats. The mechanism may be through activation of PPARγ, blocking the activation of NF-κB signaling pathway, and down-regulating inflammatory factors such as TNF-α and IL-1β. The expression is such that the inflammatory response is alleviated or eliminated, thereby achieving the therapeutic effect of UC.

3D anatomy and flow dynamics of net-depositional cyclic steps on the world’s largest submarine fan:a joint 3D seismic and numerical approach

Supercritical flows are ubiquitous in natural environments;however,there is rare 3D anatomy of their deposits.This study uses high-quality 3D seismic datasets from the world’s largest submarine fan,Bengal Fan,to interpret 3D architectures and flow processes of Pliocene undulating bedforms that were related to supercritical flows.Bengal undulating bedforms as documented in this study were developed in unconfined settings,and are seismically imaged as strike-elongated,crescentic bedforms in plan view and as rhythmically undulating,upstream migrating,erosive,discontinuous reflections in section view.Their lee sides are overall 3 to 4 times steeper(0.280 to 1.19°in slope)and 3 to 4 times shorter(117 to 419 m in length)than their stoss flanks and were ascribed to faster(high flow velocities of 2.70 to 3.98 m/s)supercritical flows(Froude numbers of 1.53 to 2.27).Their stoss sides,in contrast,are overall 3 to 4 times gentler(0.120 to 0.270 in slope)and 3 to 4 times longer(410 to 1139 m in length)than their lee flanks and were related to slower(low velocities of 2.35 to 3.05 m/s)subcritical flows(Froude numbers of 0.58 to 0.97).Bengal wave-like features were,thus,created by supercriticalto-subcritical flow transformations through internal hydraulic jumps(i.e.,cyclic steps).They have crests that are positive relative to the surrounding region of the seafloor,suggesting the predominant deposition of draping sediments associated with net-depositional cyclic steps.Turbidity currents forming Bengal wave-like features were,thus,dominated by deposition,resulting in net-depositional cyclic steps.Sandy deposits associated with Bengal net-depositional cyclic steps are imaged themselves as closely spaced,strike-elongated high RMS-attribute patches,thereby showing closely spaced,long and linear,strike-elongated distribution patterns.

Comparative Study on the Infrared Radiance Field of Building Surface under Sunshine Conditions and Rainfall Conditions

The Monte Carlo method and the region method are combined in this paper,where a high-rise building in Harbin were taken for example to investigate the infrared radiation field of building surface.The calculation models of temperature field and radiation transfer coefficient on the exterior surface were established.Through the self-built Bidirectional Reflectance Distribution Function(BRDF)experimental device,the BRDFs of moorstone,aluminum plate,coated glass and Ethylene-Propylene-Diene Monomer(EPDM)on the surfaces under dry and wet conditions were measured at different incidence angles with respective laser wavelengths of0.6328μm and 1.34μm.For the two wavelengths,the reflection ratios of the material surfaces under dry and wet conditions were calculated respectively.Based on some proper simplifications of the background condition,the simulation analysis of the radiation field of building surface was carried out according to the wetness theory and the measured data.Taking the situation at 9:00 a.m.on the day of summer solstice as an example,this paper made relevant quantitative calculation for the solar radiation,the self radiation of the surface units,the radiating projection between the surface units,and the general infrared radiation of the building surface.Comparisons on infrared radiance field of the building surface were obtained under cloud-free sunshine conditions and rainfall conditions respectively,and the rationality of the results was discussed.

Clinical experience of Professor Xie Jingri in the deficiency-cold of spleen and stomach type of complex peptic ulcer

Objective:To summarize Professor Xie's clinical experience in treating the deficiency cold of spleen and stomach type of complex peptic ulcer.Methods:through the observation of the curative effect of the patients with the deficiency cold of spleen and stomach type of complex peptic ulcer in the outpatient department of liver,spleen and stomach department of our hospital,the dialectical application and clinical experience of Professor Xie Jingri's self-made prescription in clinical practice were summarized.Results:Professor Xie Jingri's self-made prescription can effectively improve the clinical symptoms of patients with the deficiency cold of spleen and stomach type of complex peptic ulcer,and through individual syndrome differentiation and treatment,make the self-made prescription vary from person to person,clinical addition and subtraction,so as to further improve the clinical efficacy of patients.Conclusion:Professor Xie Jingri's self-made prescription is effective in treating complex peptic ulcer of spleen stomach deficiency cold type,which is worthy of further clinical application and promotion.