Effects of different processing methods on the quality of Polygonatum cyrtonema Hua

Background:In this study,we explored the effects of different processing methods on the quality of Polygonatum cyrtonema Hua(PC),and the role of Huangjiu in the processing procedure.Methods:The sensory characteristics of the crude product,steamed product,and wine-processed product of PC were described.The colorimeter was used to analyze the chromatic values of three different processed products on PC.At the same time,the contents of the water extract and alcohol extract were measured separately.The content of three different processing Polygonatum Polysaccharide(PCP)was determined using 0.2%anthrone-sulfuric acid.The correlation difference between the chromatic values and chemical composition of different PC products was analyzed using various analytical methods.Results:The surface colors gradually deepened,the sweetness increased,the viscosity strengthened,and the tongue-numbing sensation disappeared after PC processing.The contents of extract and L^(*) gradually decreased from the crude to the steamed to the wine-processed product,consistent with the pattern of surface color alteration.While,E^(*)ab gradually increased.The content of PCP was crude product>wine-processed product>steamed product.The results of multivariate statistical analysis showed that the samples processed for crude,steamed,and wine-processed product were clustered into three classes.The correlation analysis showed that L^(*)and E^(*)ab were highly significant positively correlated with the content of PCP,and a*was significantly negatively correlated with the content of PCP.Conclusion:The results showed that the wine-processed product had the best quality.The internal quality of the PC was correlated with its characteristics and chromatic value.In this study,we investigated the internal and external quality of three different products of PC in order to provide a reference for further research on the impact of different processing methods on PC quality,the standardization of PC processing,and the role of Huangjiu in the processing of PC.

Study on the regulatory effect of liver X receptor in HEK293 cells by six main diterpene esters in Semen Euphorbiae

Background:To study the effects of the main diterpene esters in Euphorbia factor L_(1),L_(2),L_(3),L_(7a),L_(7b)and L_(8)on the transcriptional activity and protein expression of liver X receptor(LXR).Methods:The effect of the main diterpene ester components in Semen Euphorbiae on the viability of HEK293 cells were studied by MTT assay.The LXR-Luc plasmid vector was transfected into HEK293 cells and treated with Euphorbia factor L_(1),L_(2),L_(3),L_(7a),L_(7b)and L_(8)for 24 h.The effect of the main diterpene ester components of Semen Euphorbiae on LXR-Luc luciferase activity was investigated by dual luciferase reporter gene system,and the expression of LXRαprotein was detected by Western Blot.Results:Euphorbia factor L_(1),L_(2),L_(3),L_(7a),L_(7b)and L_(8)could significantly reduce the relative luciferase activity(RLU)of LXRα,and the expression level of LXRαprotein was significantly down-regulated.Conclusion:Euphorbia factor L_(1),L_(2),L_(3),L_(7a),L_(7b)and L_(8)can inhibit the expression of LXR protein level,which may be achieved by inhibiting the transcriptional activity of LXR.

Study on the processing technology of Polygonatum cyrtonema Hua decoction pieces based on softening method and drying method

Background:To optimize the steaming processing technology of Polygonatum cyrtonema Hua(PC)decoction pieces.Methods:The softening method and drying method of PC decoction pieces were studied with Polygonatum Polysaccharide(PCP),diosgenin combined extract as quantitative indexes.The process parameters such as softening time,drying temperature and drying time were determined,and the best processing technology of PC decoction pieces was optimized.Results:Among the three softening methods of PC,the infiltration method had the highest ranking,with an average comprehensive index of 0.9496,and the softening effect was the best.Among the three drying methods,the drying effect of the hot air drying method was the best,and the average comprehensive index was 0.8233.Conclusion:The infiltration method is the best softening method for PC decoction pieces,and the hot air drying method is the best drying method for PC decoction pieces.